scholarly journals Efikasi Beberapa Agens Hayati Terhadap Penekanan Pertumbuhan Pyricularia grisea Secara In Vitro

2021 ◽  
Vol 23 (1) ◽  
pp. 31
Author(s):  
Shyntiya Ayu Lestari ◽  
Umi Kalsum ◽  
Evan Purnama Ramdan
Keyword(s):  
Negative Impact ◽  
Culture Method ◽  
Biological Agents ◽  
Biological Agent ◽  
Blast Disease ◽  
Pyricularia Grisea ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Chemical Pesticides

<p>The demand for rice as an important food crop in Indonesia is still constrained by the attack of blast disease caused by <em>Pyricularia grisea</em>. Controlling using synthetic chemical pesticides has a negative impact on the environment so that the use of biological agents is an alternative option. This study aims to determine the effectiveness of several biological agents against the pathogen <em>P. grisea</em> that causes blast disease in rice plants in vitro. The study used a completely randomized design (CRD) with 6 levels of treatment and was repeated 3 times using the dooble culture method. The results of the study showed that the inhibition of <em>P. grisea</em> was the highest in the treatment of <em>P. grisea</em> × <em>Trichoderma</em> sp. and <em>P. grisea</em> × <em>Gliocladium</em> sp. namely 67.04% and 51.85% compared to other treatments. The <em>P. polymyxa</em> and <em>P. fluorescence</em> treatments showed low inhibition, namely 23.70% and 28.89%. Biological agents <em>Trichoderma</em> sp. and <em>Gliocladium</em> sp. able to inhibit the growth of the fungus <em>P. grisea</em>. Each biological agent has a different percentage of inhibition in that caused by the inhibitory mechanism of the biological agent.</p>

scholarly journals Potensi Agen Hayati dalam Menghambat Pertumbuhan Phytium sp. secara In Vitro

2016 ◽  
Vol 17 (2) ◽  
pp. 138
Author(s):  
Liza Octriana
Keyword(s):  
Pathogenic Fungi ◽  
Culture Method ◽  
Biological Agents ◽  
Dual Culture ◽  
Central Laboratory ◽  
Tropical Fruit ◽  
Penicillium Sp ◽  
Randomized Design ◽  
Trichoderma Sp

<p>The Potential of Biological Agents to Inhibit Growth of Phytium sp. In Vitro. The study aimed at testing the potential of some antagonistic fungi isolated from durian seedlings media to inhibit growth of Phytium sp. Research was done at the Central Laboratory of Tropical Fruit Research Solok in July-September 2009 by using a complete randomized design with 5 treatments and 4 replications. Tests was conducted by dual culture method on PDA. The results showed that Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can inhibit growth of Phytium sp., with growth inhibition of 50, 49.5, 47, 48, and 38.3% respectively. Inhibition mecanism of Gliocladium sp., and Trichoderma sp. were competition, antibiosis, lisis, and parasitism, while Penicillium sp. was antibiosis. Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can be used as biological agents to control pathogenic fungi Phytium sp.</p><p> </p><p><strong>Abstrak</strong></p><p>Penelitian bertujuan untuk menguji potensi beberapa cendawan antagonis hasil isolasi dari media pembibitan durian dalam menghambat pertumbuhan Phytium sp. Penelitian dilakukan di Laboratorium Balai Penelitian Tanaman Buah Tropika Solok pada bulan Juli-September 2010. Penelitian disusun dalam rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan. Pengujian daya antagonis cendawan dilakukan dengan metode dual culture yang diinokulasikan pada media PDA. Hasil penelitian menunjukkan bahwa Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b., Aspergilus sp., dan Penicillium sp. dapat menghambat pertumbuhan Phytium sp. secara in vitro, dengan daya hambat masing-masing 50; 49,5; 47; 48; dan 38,3% secara berurutan. Mekanisme antagonis Gliocladium sp. dan Trichoderma sp. adalah kompetisi, antibiosis, lisis, dan parasitisme, sedangkan Penicillium sp. hanya bersifat antibiosis. Gliocladium sp., Trichoderma sp., Aspergilus sp., dan Penicillium sp. dapat digunakan sebagai agen hayati untuk mengendalikan cendawan patogen Phytium sp.</p>

The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro

2012 ◽  
Vol 10 (2) ◽  
pp. 54-60
Author(s):  
IRMA PUTRI HAYANTI ◽  
RETNA BANDRIYATI AMIPUTRI ◽  
PRASWANTO PRASWANTO
Keyword(s):  
Shoot Growth ◽  
Organic Fertilizer ◽  
Centella Asiatica ◽  
Culture Method ◽  
Shoot Formation ◽  
Randomized Design ◽  
Leaf Formation ◽  
Medicinal Crop ◽  
Completely Randomized Design

Hayanti IP, Amiputri RB, Praswanto. 2012. The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro. Biofarmasi 10: 54-60. Gotu kola (Centella asiatica L.) is a medicinal crop, needed by local industry of pharmacy. Gotu kola in Indonesia is not enough to supply in market requisites. Tissue culture method was used to multiply gotu kola with a combination of medium from liquid organic fertilizer and pearl grass extract. The purposes of the research were to determine the effect of liquid organic fertilizer and pearl grass extract on the shoot growth of gotu kola in vitro, and to obtain the optimal concentration of liquid organic fertilizer and pearl grass extract combination on the shoot formation of gotu kola in vitro. The research was conducted in November 2010 to June 2011 in Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, Surakarta. The experimental design used was a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was liquid organic fertilizer concentrations, i.e. 0 mL/L, 2 mL/L, 4 mL/L and 8 mL/L. The second factor was pearl grass extract, i.e. 0 mL/L, 3 mL/L, 6 mL/L and 12 mL/L. Variables observed were the percentage of shoot formation, the time of shoot formation, the number of shoot, the length of shoot, the percentage of leaf formation, the time of leaf formation and the number of leaf. The result of the research showed that not all of treatments provided shoot formation. The combination of liquid organic fertilizer concentrations 0 mL/L and pearl grass extract 12 mL/L showed the best results, in which the average of length of shoot was 2.16 cm, the number of shoot was 8 and the number of leaf was 8.

scholarly journals Keefektifan Bioinsektisida Berbasis Cendawan Entomopatogen Talaromyces pinophilus dan Minyak Nabati terhadap Hama Penggerek Buah Kopi

2020 ◽  
Vol 7 (2) ◽  
pp. 93
Author(s):  
Khaerati Khaerati ◽  
Gusti Indriati ◽  
Edi Wardiana
Keyword(s):  
Vegetable Oil ◽  
Biological Agents ◽  
Carrier Material ◽  
Randomized Design ◽  
Coffee Plants ◽  
Completely Randomized Design ◽  
And Control ◽  
Crop Research ◽  
Talaromyces Pinophilus

<em>The coffee berry borer (CBB) Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), is one of the main pests that cause yield losses in coffee plants. This pest directly attacks the coffee berries, both immature and ripe. The entomopathogenic fungi is one of the potential biological agents for CBB, environmentally friendly and does not kill non-target organisms. The study was conducted at the Integrated Laboratory, Industrial and Beverage Crop Research Institute, from June to October 2018. The aim of this study was to examine the effectiveness of bioinsecticide based on entomopathogenic fungus of Talaromyces pinophilus (EFTP) and vegetable oil on CBB. The completely randomized design with 31 treatments and 3 replications was used in this study. The 31<sup>th</sup> of treatments consisted of 9 formulas of bioinsecticide based on EFTP and vegetable oil, control-positive (bioinsecticide based on EFTP and water), and control- negative (Klorpirifos insecticide). Variables observed were the activities of EFTP enzyme, effectiveness of vegetable oil as a carrier material, mortality of CBB beetles, percentage of CBB attacks, percentage of hollow berries, and number of holes per 10 coffee berries. Results showed that the formula of bioinsecticide based on EFTP produce the chitinase and protease enzymes which were potential as biological agents to control CBB in vitro. Soybean oil is the most effective as a carrier material in formulating EFTP. The formula of S69MK30 and S79MK20 in concentration of 7.50% respectively have the highest potential as biological agents to control CBB. Other potential formula is S69MKT30 in concentration of 7.50%.</em>

Efek Pemberian Daun Sirsak (Annona Muricata L.) Sebagai Insektisida Botani Terhadap Mortalitas Sitophilus oryzae

Agrologia ◽  
2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Debby Moniharapon ◽  
Maria Nindatu ◽  
Faustinus Sarbunan
Keyword(s):  
Mortality Rate ◽  
Negative Impact ◽  
Sitophilus Oryzae ◽  
Natural Sciences ◽  
Annona Muricata ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Chemical Pesticides

The use of pesticides botanists to be the solution due to the use of chemical pesticides in controlling pests unwise caused negative impact on the environment. This study aims to determine the effectiveness of the leaves of the Annona muricata on mortality of Sitophilus oryzae. Research conducted at the Laboratory of Taxonomy Department of Biology, Faculty of Mathematics and Natural Sciences Ambon. The treatments tested was the heavy leaves of the soursop, consists of five levels, namely 0 g, 20 g, 25 g, 30 g and 35 g, using a completely randomized design with four replications. The results showed that showed that the treatment leaves of the Annona muricata which is effective against Sitophilus oryzae mortality was 35 g with a mortality rate reached 78.31%.

scholarly journals Production of reserpine of rauwolfia serpentina [L] kurz ex benth through in vitro culture enriched with plant growth regulators of NAA and kinetin

2018 ◽  
Vol 7 (2.29) ◽  
pp. 274 ◽  
Author(s):  
Muhammad Subandi ◽  
Dikayan . ◽  
Efrin Firmansyah
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
High Performance ◽  
Culture Method ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Institute Of Technology ◽  
Design Weight

Rauwolfia serpentine [L] Kurz ex Benth is a valuable plant yielding alkaloid of reserpine. The aim of this study was to find the most effective way of reserpine production. Growth regulators {naphthalene acetic acid [NAA] and Kinetin}were applied to promote and to regulate the growth of explant of R. serpentine cultured in vitro. The study was conducted at the laboratory of Bandung Institute of Technology [ITB]. Nine formulations of NAA and Kinetin were the treatments that were repeated twice in completely randomized design. Weight of callus was measured at one, two, three and four weeks after induction of explant. Reserpine analysis was performed by High Performance Liquid Chromatography [HPLC]. The result showed that the best callus induction was the treatment of 2.5 ppm NAA + 2.5 ppm Kinetin, and the highest content of reserpine was in root organ [0.0021 g / L], and in callus [0.0021 g / L] at the age of 4 weeks after induction. There was revealed that in vitro culture method was more productive in producing reserpine compound than the conventional plantation of R serpentine.  Production of reserpine by callus culture was more effective and may be the basic for recommended further effort.  

scholarly journals The Effect of Gibberellic Acid (GA3) On The In Vitro Seed Germination of Manggosteen (Garcinia mangostana)

el–Hayah ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 144-151
Author(s):  
Andini Andini ◽  
Riska Aqidatud Dzaroini ◽  
Ruri Siti Resmisari
Keyword(s):  
Slow Growth ◽  
Culture Method ◽  
Ms Medium ◽  
Garcinia Mangostana ◽  
Randomized Design ◽  
Tissue Culture Method ◽  
Long Time ◽  
Completely Randomized Design ◽  
Seeds Germination

Mangosteen is considered as fruit of many benefits with great popularity, rendering it valuable as an export commodity. Unfortunately, it has a relatively slow growth  and long early fruiting time. Generative propagation of mangosteen by seed result in low-yielding productionof qualified seedswith recalcitrant nature which means it cannot be stored for a long time. Whereas the result of vegetative propagation using grafting, branch cuttings and budding has difficulty rooting out. Tissue culture method requires growth regulators to control organogenesis and morphogenesis so it can accelerate the growth of the mangosteen explants. This study was arrangedintwo- factor completely randomized design (CRD). The observed variables werethe strength of the medium (½ and 1 MS) and GA3 concentrations (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm and 1 ppm). The variatonson GA3 concentration (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm; 1 ppm) on  different strength MS medium (½ MS and 1 MS) did not significantly affect the growth of radicle and sooth in the  in vitro seeds germination of Mangosteen (Garcinia mangostana).

scholarly journals Pengaruh Kombinasi Pseudomonad Flouresen Dan Em4 Dalam Menghambat Pertumbuhan Blood Disease Bacteria (Bdb) Penyebab Penyakit Darah Tanaman Pisang Secara In Vitro

BioScience ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 70
Author(s):  
Maemunah Maemunah
Keyword(s):  
Inhibition Zone ◽  
Biological Agents ◽  
Antimicrobial Compounds ◽  
Clear Zone ◽  
Blood Disease ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Paper Disc ◽  
Range Test

Blood Disease Bacteria is one obstacle in cultivation of bananas. BDB can be controlled using biological agents. Biological agents that can be used to control of blood diseases are Pseudomonad fluorescent and microorganisms in EM4. Pseudomonad fluorescent used as biological agents because of its ability to produce antimicrobial compounds such as siderophores, antibiotics, volatile and cyanide. other than that, the EM4 microorganisms can produce antimicrobial against several bacterial pathogens. This study aims to determine the effect of combination Pseudomonad fluorescent and EM4 to inhibit the growth of BDB in vitro. This research was conducted in January 2016 in the Laboratory of Microbiology Department of Biology FMIPA UNP. Research using a completely randomized design (RAL) with 6 treatments and 3 replications. The study treatment is a combination of Pseudomonads fluorescent and EM4. The observation was done by measuring the clear zone formed around the paper disc. Data were analyzed using analysis of variance (ANOVA) and the test of Duncan New Multiple Range Test (DNMRT) at 5% level. The research proves that the combination resulted in inhibition zone around the paper disc. Inhibition zone shows that the combination inhibits the growth of BDB in vitro. Inhibition zone is formed as fluorescent Pseudomonads and microbes on EM4 produce secondary metabolites that can inhibit the growth of BDB.Key Word: Pseudomonad flourecent, BDB, EM4

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

PERBANDINGAN DAYA HAMBAT BAKTERI Pseudomonas fluorescens ASAL TOMAT, KEDELAI, DAN JAGUNG TERHADAP Ralstonia solanacearum SECARA IN VITRO

Agrotek ◽  
2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Aco Roni Kirihio ◽  
Ivonne Fitria Mariay ◽  
Cipta Meliala
Keyword(s):  
Pseudomonas Fluorescens ◽  
Filter Paper ◽  
Ralstonia Solanacearum ◽  
Randomized Design ◽  
Completely Randomized Design

<em>Inhibition of Pseudomonas fluorescens isolates the origin of tomato, soybean and corn against Ralstonia solanacearum tested using a completely randomized design (CRD).        P. fluorescens growth was measured at King's B medium by way of suspension antagonist put on filter paper of 0.5 cm in diameter. Inhibition of P. fluorescens is done by placing the antagonist suspension of 0.5 cm diameter filter paper on NA media that has been deployed R. solanacearum. The results showed that: (a) the growth of P. fluorescens origin of tomato, soybean and corn on King's B media were not significantly different, (b) the inhibition of P. fluorescens isolates against R. solanacearum not significantly different and, (c) the inhibition of isolates P. fluorescens origin of tomato, soybean and corn against R. solanacearum in vitro relatively strong</em>

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