scholarly journals Borehole water: a potential health risk to rural communities in South Africa

2018 ◽  
Vol 19 (1) ◽  
pp. 128-136 ◽  
Author(s):  
S. Taonameso ◽  
L. S. Mudau ◽  
A. N. Traoré ◽  
N. Potgieter
Keyword(s):  
Drinking Water ◽  
Rural Communities ◽  
Water Samples ◽  
Rural Areas ◽  
Total Coliform ◽  
Water Sources ◽  
Potential Health ◽  
Point Mapping ◽  
E Coli ◽  
Mapping Tool

Abstract Sporadic outbreaks of diarrhoea in children in the Vhembe rural areas could be an indication of contamination in drinking water sources. In areas where improved water sources are used, not all rural households experience the benefits of these improved water sources. Water samples were collected from boreholes in three wards in the Vhembe District to determine microbiological risks over a 5-month period. A Water Point Mapping tool was used to indicate the borehole distribution. Water samples were taken from each functional borehole and analysed for total coliform and Escherichia coli counts, electrical conductivity, pH and temperature. A multiplex PCR protocol was used for identification of pathogenic E. coli. A total of 125 boreholes were identified of which only 12 were functional. Seven boreholes tested positive for total coliforms and E. coli counts. Four boreholes (33.3%) tested positive for diarrhoeagenic E. coli. Fifty-eight percent (58%) of water samples were without health risks, 17% were low risk and 25% could cause infection according to the South African water quality standards. This study indicated the importance of the role of the Municipalities and the maintenance plans that need to ensure that all boreholes are functional and provide safe drinking water to the rural communities.

scholarly journals Drivers of microbiological quality of household drinking water – a case study in rural Ethiopia

2017 ◽  
Vol 16 (2) ◽  
pp. 275-288 ◽  
Author(s):  
Muhammed A. Usman ◽  
Nicolas Gerber ◽  
Evita H. Pangaribowo
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Rural Areas ◽  
Fecal Contamination ◽  
Microbiological Quality ◽  
Water Sources ◽  
Multiple Use ◽  
Water Safety ◽  
E Coli

Abstract This study aims at assessing the determinants of microbiological contamination of household drinking water under multiple-use water systems in rural areas of Ethiopia. For this analysis, a random sample of 454 households was surveyed between February and March 2014, and water samples from community sources and household storage containers were collected and tested for fecal contamination. The number of Escherichia coli (E. coli) colony-forming units per 100 mL water was used as an indicator of fecal contamination. The microbiological tests demonstrated that 58% of household stored water samples and 38% of protected community water sources were contaminated with E. coli. Moreover, most improved water sources often considered to provide safe water showed the presence of E. coli. The result shows that households' stored water collected from unprotected wells/springs had higher levels of E. coli than stored water from alternative sources. Distance to water sources and water collection containers are also strongly associated with stored water quality. To ensure the quality of stored water, the study suggests that there is a need to promote water safety from the point-of-source to point-of-use, with due considerations for the linkages between water and agriculture to advance the Sustainable Development Goal 6 of ensuring access to clean water for everyone.

scholarly journals Abundance of pathogenic Escherichia coli, Salmonella typhimurium and Vibrio cholerae in Nkonkobe drinking water sources

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Salmonella Typhimurium ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Water Sources ◽  
E Coli ◽  
Drinking Water Sources

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.

scholarly journals Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water

2014 ◽  
Vol 13 (2) ◽  
pp. 340-352 ◽  
Author(s):  
Andrée F. Maheux ◽  
Vanessa Dion-Dupont ◽  
Sébastien Bouchard ◽  
Marc-Antoine Bisson ◽  
Michel G. Bergeron ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Ease Of Use ◽  
Well Water ◽  
Positive Signal ◽  
Culture Methods ◽  
E Coli

The MI agar, Colilert®, Chromocult coliform® agar, and DC with BCIG agar chromogenic culture-based methods used to assess microbiological quality of drinking water were compared in terms of their ubiquity, sensitivity, ease of use, growth of atypical colonies and affordability. For ubiquity, 129 total coliform (representing 76 species) and 19 Escherichia coli strains were tested. Then, 635 1-L well water samples were divided into 100 mL subsamples for testing by all four methods. Test results showed that 70.5, 52.7, 36.4, and 23.3% of the non-E. coli total coliform strains and 94.7, 94.7, 89.5, and 89.5% of the 19 E. coli strains yielded a positive signal with the four methods, respectively. They also yielded a total coliform positive signal for 66.5, 51.7, 64.9, and 55.0% and an E. coli positive signal for 16.1, 14.8, 17.3, and 13.4% of the 635 well water samples tested, respectively. Results showed that Colilert® is the most expensive method tested in terms of reactants, yet it is the easiest to use. Large numbers of atypical colonies were also often observed on Chromocult coliform® and DC with BCIG, thereby challenging the target microorganism count. Thus, the MI agar method seems to be the best option for the assessment of drinking water quality.

scholarly journals Quality and Safety of Rural Community Drinking Water Sources in Guto Gida District, Oromia, Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Motuma Tessema Abegaz ◽  
Mulissa Jida Midekssa
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Rural Community ◽  
Water Samples ◽  
Water Source ◽  
Total Coliform ◽  
Water Sources ◽  
Iron Manganese ◽  
Dug Well

The quality of drinking water has always been a major public health concern, especially in developing countries where access to improved water supply and sanitation is very low. This study aimed to assess the bacteriological and physicochemical quality of rural community drinking water sources in the Guto Gida district. A cross-sectional study was conducted in selected rural areas of the district from January to June 2016. Water samples were collected from four types of sources (protected dug well, open dug well, protected spring, and open spring) found in 8 locations of the study area. The membrane filtration technique was employed to determine the total coliform and faecal coliform load of the samples. The physicochemical characteristics such as total dissolved solid (TDS), pH, electrical conductivity (EC), turbidity, temperature, color, iron, manganese, lead, fluoride, zinc, sulphate, nitrate, and phosphate were analyzed following the American Public Health Association and WHO standard protocols. Our results revealed that 90.6% and 87.5% of water samples were positive for total coliform and faecal coliform, respectively. Thus, the majority of the studied water sources could be classified as polluted with respect to coliform load. Our results also have shown that most of the water sources showed marginally tolerable quality with respect to color, EC, TDS, turbidity, nitrate, sulphate, and phosphate. However, the protected sources had poor quality in zinc, lead, iron, manganese, and pH with values above the permissible levels. Thus, the drinking water source quality of the study areas requires appropriate interventions such as improving the existing water source infrastructure and access to sanitation services.

scholarly journals A comparison between minerals-modified glutamate medium and lauryl tryptose lactose broth for the enumeration of Escherichia coli and coliform organisms in water by the multiple tube method

1980 ◽  
Vol 85 (1) ◽  
pp. 35-49 ◽  
Author(s):  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Total Coliform ◽  
Faecal Contamination ◽  
E Coli ◽  
Chlorinated Drinking Water ◽  
Laboratory Trial ◽  
Gas Reactions ◽  
Better Than

SummaryIn a multi-laboratory trial, minerals-modified glutamate medium (MMGM) was compared with lauryl tryptose lactose broth (LTLB) in the multiple tube method for the enumeration of coliform organisms, including Escherichia coli, in water. Samples of raw and chlorinated waters yielded a total of 2313 positive tube-reactions with MMGM and 2174 with LTLB. These were interpreted either as E. coli; other coliform organisms; or as false positive reactions. The results at first reading (18 or 24 h) and at 48 h have been analysed statistically in terms of (i) most probable numbers of coliform organisms; (ii) positive reactions and their interpretation; and (iii) whether or not the sample yielded any E. coli or other coliform organisms. All three analyses indicated the same trends. For the detection of E. coli in raw waters LTLB was better than MMGM at 18–24 h, but MMGM was better at 48 h with waters containing small numbers of coliform organisms; for raw waters with greater numbers of organisms, both media performed equally well. Analysis of a subset of samples read at both 18 and 24 h indicated that the superiority of LTLB over MMGM with raw waters disappeared by 24 h. For chlorinated waters, LTLB yielded more positive gas reactions at 18–24 h, but fewer of these were E. coli than with MMGM; at 48 h MMGM was clearly better than LTLB for total coliform organisms including E. coli – especially if the numbers were small. MMGM therefore remains the medium of choice for the detection of E. coli as an indicator of faecal contamination of chlorinated drinking water supplies. It is also better for the detection of small numbers of E. coli in other waters.

scholarly journals Concentration Detection of the E. coli Bacteria in Drinking Water Treatment Plants through an E-Nose and a Volatiles Extraction System (VES)

Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 774
Author(s):  
Jeniffer Carrillo-Gómez ◽  
Cristhian Durán-Acevedo ◽  
Ramón García-Rico
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Water Samples ◽  
Drinking Water Treatment ◽  
Extraction System ◽  
Water Quality Control ◽  
Potential Health ◽  
E Coli ◽  
Water Treatment Plants

Water quality control remains an important topic of public health since some diseases, such as diarrhea, hepatitis, and cholera, are caused by its consumption. The microbiological quality of drinking water relies mainly on monitoring of Escherichia coli, a bacteria indicator which serves as an early sentinel of potential health hazards for the population. In this study, an electronic nose coupled to a volatile extraction system (was evaluated for the detection of the emitted compounds by E. coli in water samples where its capacity for the quantification of the bacteria was demonstrated). To achieve this purpose, the multisensory system was subjected to control samples for training. Later, it was tested with samples from drinking water treatment plants in two locations of Colombia. For the discrimination and classification of the water samples, the principal component analysis method was implemented obtaining a discrimination variance of 98.03% of the measurements to different concentrations. For the validation of the methodology, the membrane filtration technique was used. In addition, two classification methods were applied to the dataset where a success rate of 90% of classification was obtained using the discriminant function analysis and having a probabilistic neural network coupled to the cross-validation technique (leave-one-out) where a classification rate of 80% was obtained. The application of this methodology achieved an excellent classification of the samples, discriminating the free samples of E. coli from those that contained the bacteria. In the same way, it was observed that the system could correctly estimate the concentration of this bacteria in the samples. The proposed method in this study has a high potential to be applied in the determination of E. coli in drinking water since, in addition for estimating concentration ranges and having the necessary sensitivity, it significantly reduces the time of analysis compared to traditional methods.

scholarly journals Diarrhoeal Disease in Relation to Possible Household Risk Factors in South African Villages

2018 ◽  
Vol 15 (8) ◽  
pp. 1665 ◽  
Author(s):  
Thandi Kapwata ◽  
Angela Mathee ◽  
Wouter le Roux ◽  
Caradee Wright
Keyword(s):  
Risk Factors ◽  
Drinking Water ◽  
Rural Communities ◽  
Rural Areas ◽  
Diarrhoeal Disease ◽  
Total Coliform ◽  
Personal Hygiene ◽  
Household Level ◽  
Guideline Values

Diarrhoeal disease is a significant contributor to child morbidity and mortality, particularly in the developing world. Poor sanitation, a lack of personal hygiene and inadequate water supplies are known risk factors for diarrhoeal disease. Since risk factors may vary by population or setting, we evaluated the prevalence of diarrhoeal disease at the household level using a questionnaire to better understand household-level risk factors for diarrhoea in selected rural areas in South Africa. In a sub-sample of dwellings, we measured the microbial quality of drinking water. One in five households had at least one case of diarrhoea during the previous summer. The most widespread source of drinking water was a stand-pipe (inside yard) (45%) followed by an indoor tap inside the dwelling (29%). Storage of water was common (97%) with around half of households storing water in plastic containers with an opening large enough to fit a hand through. After adjusting for confounders, the occurrence of diarrhoea was statistically significantly associated with sourcing water from an indoor tap (Adjusted Odds Ratio (AOR): 2.73, 95% CI: 2.73, 1.14–6.56) and storing cooked/perishable food in non-refrigerated conditions (AOR: 2.17, 95% CI: 2.17, 1.44–3.26). The highest total coliform counts were found in water samples from kitchen containers followed by stand-pipes. Escherichia coli were most often detected in samples from stand-pipes and kitchen containers. One in four households were at risk of exposure to contaminated drinking water, increasing the susceptibility of the study participants to episodes of diarrhoea. It is imperative that water quality meets guideline values and routine monitoring of quality of drinking water is done to minimise diarrhoea risk in relevant rural communities. The security of water supply in rural areas should be addressed as a matter of public health urgency to avoid the need for water storage.

scholarly journals Bacteriological assessment of dug well water in rural areas of Bangladesh

2020 ◽  
Vol 69 (7) ◽  
pp. 720-732
Author(s):  
Md. Atikul Islam ◽  
Md. Ali Akber ◽  
M. M. Majedul Islam ◽  
Md. Azharul Islam ◽  
Mukta Dutta
Keyword(s):  
Rural Communities ◽  
Rural Areas ◽  
Total Coliform ◽  
Dry Season ◽  
Risk Category ◽  
Wet Season ◽  
Government Organization ◽  
E Coli ◽  
Dug Wells ◽  
Dug Well

Abstract This study assessed the bacteriological quality of dug well waters from Jashore district – an arsenic affected area of Bangladesh. A total of 58 dug wells (42 installed by a government organization (GO) and 16 installed by a non-government organization (NGO)) were sampled in the dry and wet seasons. The samples were evaluated for total coliform (TC), faecal coliform (FC) and Escherichia coli (E. coli). Sanitary inspections of the surroundings of the GO-installed dug wells identified the sources of faecal contamination. Both the GO-installed and NGO-installed dug wells had bacterial contamination. The median concentrations of E. coli among the GO-installed and NGO-installed dug wells were, respectively, 41 and 21 cfu/100 ml in the wet season, and respectively 2 and <1 cfu/100 ml in the dry season. In the wet season, 24 and 31%, respectively, of the GO-installed and NGO-installed dug wells were in the high-risk category. All of the dug wells had higher disease burden in the wet season compared to the dry season. The findings suggest that drinking water from the dug wells is likely to pose health risks to the rural communities.

scholarly journals Evaluation of Multi-Drug Resistant E. coli from Drinking Water Sources in a Tertiary Institution

2020 ◽  
pp. 1-9
Author(s):  
Udoh I.P., Iloghalu ◽  
Iloghalu, Ijenwa Amarachi ◽  
Aladenika S.T.
Keyword(s):  
Drinking Water ◽  
Multidrug Resistance ◽  
Water Samples ◽  
Antimicrobial Agents ◽  
Water Sources ◽  
Bottled Water ◽  
Health Concern ◽  
Storage Tanks ◽  
E Coli ◽  
Drinking Water Sources

Infectious diseases control of recent is a major health concern globally due to high increase in number of microorganisms that are resistant to conventional antimicrobial agents. This study aimed at ascertaining the microbiological quality and multiple antibiotic resistance profile of E. coli strains isolated from different sources of drinking water. A total of 136 water samples from different drinking water sources, including the storage tanks (the school and the commercial storage tank), sachet and bottle water were obtained from University of Nigeria Enugu Campus and analyzed. Standard microbiological techniques were employed for bacteria isolation, identification and antibiogram. From the water samples collected 25 E. coli strains were isolated. The school storage tanks account for 60% of the isolates, while bottled water showed no growth. 92% of the E. coli isolated showed resistance to the tested antibiotics. Resistant were higher with Augmentin (64%), Chloramphenicol (48%) and Streptomycin 11 (44%) while most were sensitive to Tarivid and Perfloxacine (100%). Isolates from school storage water sources showed the highest resistance to Augmentin (76.5%) while those from commercial storage water sources showed the highest resistance to Streptomycin (66.7%). Out of the 23 (92%) antibiotics resistant E. coli isolates 18 (78.3%) were multidrug resistance (MDR). The school storage water sources had the highest number of MDR E. coli 14 (77.8%) followed by the commercial storage water sources 4 (22.2%), but the sachet and bottled water had no MDR E. coli. In conclusion, drinking water may potentially contribute to the source of multidrug resistance E. coli in this community

scholarly journals Assessment of microbial contamination of drinking water with total coliform bacteria and Escherichia coli in the Bitola region

2019 ◽  
Vol 65 (2) ◽  
pp. 23-32
Author(s):  
Marta Nedelkova ◽  
Angela Delova ◽  
Tanja Petreska Ivanovska ◽  
Zoran Zhivikj ◽  
Lidija Petrushevska-Tozi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Supply ◽  
Rural Areas ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Quality And Safety ◽  
E Coli ◽  
Total Coliform Bacteria

The aim of this paper is to analyze the presence of Escherichia coli (E. coli) and total coliform bacteria (CB) in the drinking water of urban and rural areas of the Bitola region, as indicators for water quality and safety. All water in urban area is chlorinated, while at the same time the water in rural areas is non-chlorinated. The samples were analyzed according to the international standard method of membrane filtration MKC EN ISO 9308-1:2015. In all examined samples of drinking water in urban area, presence of E. coli and CB was not detected as a result of the disinfection of the water. On contrary, in all tested samples of the water from the rural water supply, presence of E. coli and CB was confirmed. Significant increase in coliform bacterial counts probably weather-related was found in the period from April to September. In addition, in the third quarter including July, August, and September, in many measurements, E. coli as an indicator of faecal contamination was identified in drinking water. In accordance with these findings and in order to provide safe drinking water, it is necessary to modernize the water supply for the population in rural areas, to disinfect permanently the drinking water and to apply regular laboratory controls which are a basic pre-condition. Otherwise, inappropriate management of the water systems can cause serious decrease in the quality and safety of the drinking water associated with an increased risk of appearance of the infectious diseases in people and hydric epidemic. Key words: drinking water, Escherichia coli, coliform bacteria, membrane filtration

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