Effect of Aqueous Extract of Launaea acanthodes on Testicular Tissue and Sperm Parameters in Alloxan-Induced Diabetic Rats

AbstractStructural and physiological changes in sperm and semen parameters reduce fertility in diabetic patients. Securigera Securidaca (S. Securidaca) seed is a herbal medicine with hypoglycemic, antioxidant, and anti-hypertensive effects. The question now is whether this herbal medicine improves fertility in diabetic males. The study aimed to evaluate the effects of hydroalcoholic extract of S. Securidaca seeds (HESS), glibenclamide and a combination of both on fertility in hyperglycemic rats by comparing histological and some biochemical changes in testicular tissue and sperm parameters. The treatment protocol included administration of three doses of HESS and one dose of glibenclamide, as well as treatment with both in diabetic Wistar diabetic rats and comparison of the results with untrated groups. The quality of the testicular tissue as well as histometric parameters and spermatogenesis indices were evaluated during histopathological examination. Epididymal sperm analysis including sperm motility, viability, abnormalities, maturity, and chromatin structure were studied. The effect of HESS on the expression of LDH and FGF21 genes and tissue levels of glycogen, lactate, and total antioxidant capacity in testicular tissue was investigated and compared with glibenclamide. HESS improved sperm parameters in diabetic rats but showed little restorative effect on damaged testicular tissue. In this regard, glibenclamide was more effective than the highest dose of HESS and its combination with HESS enhanced its effectiveness so that histological tissue characteristics and sperm parameters were were comparable to those of healthy rats. The expression level of testicular FGF21 gene increased in diabetic rats, which intensified after treatment with HESS as well as glibenclamide. The combination of HESS and glibenclamide restored the expression level of testicular LDH gene, as well as tissue storage of glycogen, lactate and LDH activity, and serum testosterone to the levels near healthy control. S. Securidaca seeds can be considered as an effective supplement in combination with hypoglycemic drugs to prevent infertility complications in diabetes.

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In vivo assessment of hypoglycaemic and antioxydant activities of aqueous extract of Terminalia superba in alloxan-diabetic rats

Planta Medica ◽

10.1055/s-0029-1234718 ◽

2009 ◽

Vol 75 (09) ◽

Author(s):

NEC Momo ◽

EJ Oben

Keyword(s):

Aqueous Extract ◽

Diabetic Rats ◽

Terminalia Superba ◽

In Vivo Assessment

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Attenuation of oxidative stress in hepatic and pancreatic tissues of STZ-induced diabetic rats treated with aqueous extract of Vochysia rufa

Planta Medica ◽

10.1055/s-0034-1394539 ◽

2014 ◽

Vol 80 (16) ◽

Cited By ~ 2

Author(s):

NM De Gouveia ◽

IB Moraes ◽

RMF Sousa ◽

MB Neto ◽

AV Mundim ◽

...

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Diabetic Rats

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Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200513081312 ◽

2020 ◽

Vol 20 (9) ◽

pp. 1504-1513 ◽

Cited By ~ 1

Author(s):

Ayoub Amssayef ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Glucose Tolerance ◽

Oral Administration ◽

Beneficial Effect ◽

Aqueous Extract ◽

Aerial Part ◽

Diabetic Rats ◽

Histopathological Analysis ◽

Phytochemical Screening ◽

Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

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Mentha pulegium Aqueous Extract Exhibits Antidiabetic and Hepatoprotective Effects in Streptozotocin-Induced Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530318666181005102247 ◽

2019 ◽

Vol 19 (3) ◽

pp. 292-301

Author(s):

Omar Farid ◽

Naoufel Ali Zeggwagh ◽

Fadwa EL Ouadi ◽

Mohamed Eddouks

Keyword(s):

Blood Glucose ◽

Glucose Tolerance ◽

Aqueous Extract ◽

Morphometric Analysis ◽

Diabetic Rats ◽

Mentha Pulegium ◽

Glucose Levels ◽

Histological Sections ◽

Blood Glucose Levels ◽

Liver And Pancreas

Objective: The aim of this work was to evaluate the antihyperglycemic activity of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rat. The glucose tolerance was evaluated in normal rats. Moreover, the histological sections and morphometric analysis at the liver and pancreas have been carried out in this investigation both in normal and STZ-diabetic rats. Methods: The effect of A.P.A.E of M. pulegium (20 mg/kg) on blood glucose levels was investigated in normal and diabetic rats (n=6). Histopathological changes in liver and pancreas were examined under phase contrast microscope and a preliminary screening for various bioactive constituents was realized according to standard methods. Key Findings: Both single and repeated oral administration of A.P.A.E (20 mg/kg) caused a significant reduction in blood glucose levels in STZ-diabetic rats (p<0.0001). The morphometric analysis and histological sections realized in pancreas and liver have showed the beneficial effect of the A.P.A.E in cellular population. According to oral glucose tolerance test (OGTT), the aqueous extract has revealed an improvement of glucose tolerance in normal rat. Furthermore, the preliminary phytochemical screening of A.P.A.E of M. pulegium has demonstrated the presence of various metabolite compounds including polyphenols, flavonoids, terpenoids tannins, cyanidins, sesquiterpenes, and glycosides. Conclusion: We conclude that the A.P.A.E of M. pulegium (20 mg/kg) exhibits a potent antihyperglycemic activity in STZ diabetic rats.

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Inheritable testicular metabolic memory of high-fat diet causes transgenerational sperm defects in mice

Scientific Reports ◽

10.1038/s41598-021-88981-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Luís Crisóstomo ◽

Ivana Jarak ◽

Luís P. Rato ◽

João F. Raposo ◽

Rachel L. Batterham ◽

...

Keyword(s):

Sperm Quality ◽

Feeding Habits ◽

Reproductive Function ◽

Principal Component ◽

Testicular Tissue ◽

Sperm Parameters ◽

Metabolic Memory ◽

High Fat ◽

And Function ◽

The Impact

AbstractThe consumption of energy-dense diets has contributed to an increase in the prevalence of obesity and its comorbidities worldwide. The adoption of unhealthy feeding habits often occurs at early age, prompting the early onset of metabolic disease with unknown consequences for reproductive function later in life. Recently, evidence has emerged regarding the intergenerational and transgenerational effects of high-fat diets (HFD) on sperm parameters and testicular metabolism. Hereby, we study the impact of high-fat feeding male mice (F0) on the testicular metabolome and function of their sons (F1) and grandsons (F2). Testicular content of metabolites related to insulin resistance, cell membrane remodeling, nutritional support and antioxidative stress (leucine, acetate, glycine, glutamine, inosine) were altered in sons and grandsons of mice fed with HFD, comparing to descendants of chow-fed mice. Sperm counts were lower in the grandsons of mice fed with HFD, even if transient. Sperm quality was correlated to testicular metabolite content in all generations. Principal Component Analysis of sperm parameters and testicular metabolites revealed an HFD-related phenotype, especially in the diet-challenged generation and their grandsons. Ancestral HFD, even if transient, causes transgenerational “inherited metabolic memory” in the testicular tissue, characterized by changes in testicular metabolome and function.

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Antidiabetic and antihyperlipidemic effect of aqueous extract of Parquetina nigrescens in streptozotocin–nicotinamide induced type 2 diabetic rats

Heliyon ◽

10.1016/j.heliyon.2021.e07363 ◽

2021 ◽

pp. e07363

Author(s):

Fatimoh Idowu Ojuade ◽

Olufunke Esan Olorundare ◽

Olatunde Akanbi ◽

Saheed Olanrewaju Afolabi ◽

Anoka Njan

Keyword(s):

Aqueous Extract ◽

Diabetic Rats ◽

Type 2 Diabetic

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Effects of ascorbic acid on spermatogenesis and sperm parameters in diabetic rats

Cell and Tissue Research ◽

10.1007/s00441-017-2660-6 ◽

2017 ◽

Vol 370 (2) ◽

pp. 305-317 ◽

Cited By ~ 10

Author(s):

M. Verónica Aguirre-Arias ◽

Victoria Velarde ◽

Ricardo D. Moreno

Keyword(s):

Ascorbic Acid ◽

Diabetic Rats ◽

Sperm Parameters

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In Vivo Subacute Toxicity and Antidiabetic Effect of Aqueous Extract of Nigella sativa

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/8427034 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 7

Author(s):

Karima Bensiameur-Touati ◽

Ghouti Kacimi ◽

El-Mehdi Haffaf ◽

Sihem Berdja ◽

Souhila Aouichat-Bouguerra

Keyword(s):

Aqueous Extract ◽

Nigella Sativa ◽

Histological Study ◽

Diabetic Rats ◽

Antidiabetic Effect ◽

Subacute Toxicity ◽

Wide Range ◽

Liver And Pancreas ◽

Liver Tbars

Context. Nigella sativa seeds are usually used as traditional medicine for a wide range of therapeutic purposes. Objective. To investigate the subacute toxicity of NS aqueous extract and select its lowest dose to study its antidiabetic effect. Methods. 5 AqE.NS doses (2, 6.4, 21, 33, and 60 g/Kg) were daily administered to mice by gavage. Biochemical parameters measurements and histological study of the liver and the kidney were performed after 6 weeks of supplementation. Thereafter, and after inducing diabetes by alloxan, rats were treated by 2 g/Kg of AqE.NS during 8 weeks. Metabolic parameters were measured on sera. A horizontal electrophoresis of plasmatic lipoprotein was conducted. Glycogen, total lipids, and triglycerides were measured in the liver. TBARS were evaluated on adipose tissue, liver, and pancreas. Results. AqE.NS showed no variation in urea and albumin at the 5 doses, but hepatotoxicity from 21 g/Kg was confirmed by histopathological observations of the liver. In diabetic rats, AqE.NS significantly decreased glycemia, TG, T-cholesterol, LDL-c, and TBARS and showed a restored insulinemia and a significant increase in HDL-c. Results on the liver indicated a decrease in lipids and a possible glycogenogenesis. Conclusion. AqE.NS showed its safety at low doses and its evident antihyperglycemic, antihyperlipidemic, and antioxidant effect.

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Chenopodium quinoa Exhibits Antihyperglycemic Activity in Streptozotocin-Induced Diabetic Rats

Cardiovascular & Hematological Agents in Medicinal Chemistry ◽

10.2174/1871525719666210812094837 ◽

2021 ◽

Vol 19 ◽

Author(s):

Amine Azzane ◽

Ayou Amssayef ◽

Mohame Eddouks

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Histopathological Examination ◽

Chenopodium Quinoa ◽

Diabetic Rats ◽

Favorable Effect ◽

Antihyperglycemic Activity ◽

Glucose Levels ◽

Blood Glucose Levels

Aims: The aim of the study was to evaluate the antihyperglycemic effect of Chenopodium quinoa. Background: Chenopodium quinoa is a pseudocereal plant with several medicinal properties. Objective: The goal of this investigation was to determine the antihyperglycemic activity of Chenopodium quinoa in both normal and streptozotocin(STZ)-induced diabetic rats. Methods: In this study, the effect of the aqueous extract of Chenopodium quinoa seeds (AECQS) (60 mg/kg) on blood glucose levels was evaluated in both normal and diabetic rats after a single (6 hours) and repeated oral administration (7 days of treatment). The effect of this herb on glucose tolerance and lipid profile was also studied. Additionally, histopathological examination of liver was carried out using the Hematoxylin-Eosin method. Furthermore, the in vitro antioxidant activity as well as a preliminary phytochemical screening and quantification of some secondary metabolites (phenolic compounds, flavonoids and tannins) were performed according to standard methods. Results: AECQS produced a significant lowering effect on plasma glucose levels in STZ-induced diabetic rats. In addition, this extract exhibited a remarkable amelioration on hepatic histopathology in diabetic rats. In addition, the extract exerted a remarkable antioxidant activity which could be due to the presence of some compounds found in this herb. Conclusion: In conclusion, this study demonstrates that the aqueous extract of Chenopodium quinoa seeds has a favorable effect in controlling diabetes mellitus.

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