Evaluation of testicular glycogen storage, FGF21 and LDH expression and physiological parameters of sperm in hyperglycemic rats treated with hydroalcoholic extract of Securigera Securidaca seeds, and Glibenclamide

AbstractStructural and physiological changes in sperm and semen parameters reduce fertility in diabetic patients. Securigera Securidaca (S. Securidaca) seed is a herbal medicine with hypoglycemic, antioxidant, and anti-hypertensive effects. The question now is whether this herbal medicine improves fertility in diabetic males. The study aimed to evaluate the effects of hydroalcoholic extract of S. Securidaca seeds (HESS), glibenclamide and a combination of both on fertility in hyperglycemic rats by comparing histological and some biochemical changes in testicular tissue and sperm parameters. The treatment protocol included administration of three doses of HESS and one dose of glibenclamide, as well as treatment with both in diabetic Wistar diabetic rats and comparison of the results with untrated groups. The quality of the testicular tissue as well as histometric parameters and spermatogenesis indices were evaluated during histopathological examination. Epididymal sperm analysis including sperm motility, viability, abnormalities, maturity, and chromatin structure were studied. The effect of HESS on the expression of LDH and FGF21 genes and tissue levels of glycogen, lactate, and total antioxidant capacity in testicular tissue was investigated and compared with glibenclamide. HESS improved sperm parameters in diabetic rats but showed little restorative effect on damaged testicular tissue. In this regard, glibenclamide was more effective than the highest dose of HESS and its combination with HESS enhanced its effectiveness so that histological tissue characteristics and sperm parameters were were comparable to those of healthy rats. The expression level of testicular FGF21 gene increased in diabetic rats, which intensified after treatment with HESS as well as glibenclamide. The combination of HESS and glibenclamide restored the expression level of testicular LDH gene, as well as tissue storage of glycogen, lactate and LDH activity, and serum testosterone to the levels near healthy control. S. Securidaca seeds can be considered as an effective supplement in combination with hypoglycemic drugs to prevent infertility complications in diabetes.

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Curcumin Along With Fe3O4 Nanoparticles Improved Sperm Parameters In Rats With Testicular Ischemia

Galen Medical Journal ◽

10.31661/gmj.v10i0.2034 ◽

2021 ◽

Vol 10 ◽

pp. e2034

Author(s):

Shabnam Zarei Moradi ◽

Seyed Abdolhamid Angaji ◽

Mitra Salehi ◽

Mehrdad Hashemi

Keyword(s):

Fe3o4 Nanoparticles ◽

Sperm Concentration ◽

Concomitant Administration ◽

Testicular Tissue ◽

Sperm Parameters ◽

Male Rats ◽

Semen Parameters ◽

Control Group ◽

Fe3o4 Nps ◽

Testicular Ischemia

Background: Ischemic/reperfusion (I/R) in testicular tissue is one reason for the worldwide increase in male infertility. In the present study, we assessed the effects of curcumin and Fe3O4 nanoparticles (NPs) on sperm parameters in rats with I/R damage. Materials and Methods: Forty-eight adult male rats were divided into two groups (n=24 per group): control and torsion/detorsion. The control and torsion/detorsion groups were divided into four subgroups include sham, Fe3O4 NPs, curcumin, and Fe3O4 NPs+curcumin. After the rats were sacrificed, semen was collected from their epididymal tissues to assess sperm viability, motility, concentration, and morphology. Results: Curcumin significantly improved viability, motility, and normal sperm morphology in rats with I/R damage compared to the control group; however, it did not have a significant effect on sperm concentration (P<0.001). Fe3O4 NPs alone decreased all sperm parameters in the control and I/R rats (P<0.001). However, concomitant administration of Fe3O4 nanoparticles with curcumin significantly improved sperm parameters in rats with I/R damage (P<0.001). Conclusion: The increase in all semen parameters in the experimental groups with concomitant use of Fe3O4 NPs plus curcumin indicated that green synthesis of NPs could be recommended for future clinical studies.

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Effect of Aqueous Extract of Launaea acanthodes on Testicular Tissue and Sperm Parameters in Alloxan-Induced Diabetic Rats

Quarterly of Horizon of Medical Sciences ◽

10.18869/acadpub.hms.21.1.21 ◽

2015 ◽

Vol 21 (1) ◽

pp. 21-29 ◽

Cited By ~ 1

Author(s):

Rahele Rohbarian ◽

Heshmat SepehriMoghadam ◽

Seyed Damoon Sadooghi ◽

◽

...

Keyword(s):

Aqueous Extract ◽

Testicular Tissue ◽

Diabetic Rats ◽

Sperm Parameters

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Evaluation of Sclerorhachis leptoclada hydroalcoholic extract on glucose and cholesterol levels, triglyceride, Serum HDL and LDL in male diabetic rats

Journal of Birjand University of Medical Sciences ◽

10.32592/jbirjandunivmedsci.2020.27.4.103 ◽

2020 ◽

pp. 344-354

Keyword(s):

Group Treatment ◽

Density Lipoprotein ◽

Serum Levels ◽

Diabetic Control ◽

Diabetic Rats ◽

The Body ◽

Control Group ◽

Antioxidant Compounds ◽

Diabetic Patients ◽

Hydroalcoholic Extract

Background and Aim: Diabetes is a metabolic disorder in the body. Sclerorhachis leptoclada belongs to the chicory family, is used to treat aids and cancer due to the antioxidant compounds in the root. Therefore, in this study, the effect of hydroalcoholic extract of Sclerorhachis leptoclada on glucose and cholesterol, triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL) levels in diabetic rats was investigated. Materials and Methods: In this experimental study, 60 male Wistar rats were randomly divided into 10 groups of 6: Control group, treatment groups 1, 2, and 3 (healthy, treated with 150 mg/kg and 300 and 600 extracts), Diabetic control group, experimental groups 1, 2 and 3 (diabetic, treated with doses of 150 mg/kg and 300 and 600 extracts), Positive control group (diabetic+glibenclamide), The healthy group (healthy+glibenclamide) was divided. After one month of gavage, blood samples were taken from the rats, and glucose, urea, creatinine, and albumin levels were measured. A comparison was made between the effect of hydroalcoholic extract of Sclerorhachis leptoclada and the common drug glibenclamide and the results of the groups were compared by using t-test and ANOVA. Results: The results showed that the administration of hydroalcoholic extract of Sclerorhachis leptoclada can significantly reduce glucose levels in experimental groups (diabetic+extract at doses of 150, 300, 600) compared with the diabetic group (p<0.001) Also, the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic patients causes a significant reduction in triglyceride, HDL and LDL levels in diabetic rats and has hypoglycemic effects. Conclusion: The results of this study showed that the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic rats significantly reduces serum levels of glucose, triglyceride and creatinine in diabetic rats and has hypoglycemic effects

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Effects of Securigera Securidaca Seed Extract, Alone and in combination with Glibenclamide, on Circulating Levels of Pro-Angiogenic/Anti-Angiogenic Biomarkers in Hyperglycemic Rats

10.21203/rs.3.rs-52508/v1 ◽

2020 ◽

Author(s):

Shahin Alizadeh-Fanalou ◽

Mohammad Babaei ◽

Elham Bahreini

Keyword(s):

Side Effects ◽

Combination Therapy ◽

Diabetes Complications ◽

Serum Levels ◽

Diabetic Rats ◽

Angiogenic Factors ◽

Herbal Extract ◽

Diabetic Patients ◽

Angiogenic Biomarkers ◽

Securigera Securidaca

Abstract Background: Many diabetic patients use herbal medicines in addition to their mainstream treatments. Plants contain a well-known and unknown set of compounds that may exacerbate or improve diabetes complications. Thus, the side effects of these herbs should be known before prescribing. The aim of the study is to investigate the effects of hydroalcoholic extract of Securigera securidaca (L.) Degen & Dorfl (S. securidaca) seed (HESS) on angiogenesis/anti-angiogenesis balance in Streptozotocin (STZ)-induced diabetic rats, alone and in combination with glibenclamide. Methods: The groups involved in this animal study included diabetic and healthy control groups, groups treated with three doses of HESS, group treated with glibenclamide, and groups received combination therapy. Serum samples were taken and analyzed for the levels of angiogenic/ anti-angiogenic biomarkers.Results: Induction of diabetes increased serum levels of angiogenic agents and decreased circulating anti-angiogenic factors. The herbal extract, even with the highest dose, had little effects on the blood levels of the tested biomarkers except with TGF-β. Glibenclamide was more effective than the highest dose of HESS in preventing the increase in serum levels of angiogenic factors and in inhibiting the decrease in anti-angiogenic agents in diabetic rats. Combination therapy with the highest dose of HESS partly enhanced the glibenclamide effects.Conclusions: Although glibenclamide was more effective than the highest dose of HESS used in this study in preventing changes in serum concentrations of angiogenic/ anti-angiogenic biomarkers in the diabetic animals, this study show that S. securidaca has no side effects on diabetes complications caused by vascular disorders and neovascularization, and still it can be used as a herbal supplement with the standard drug.

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Hyperbaric Oxygen Therapy Dampens Inflammatory Cytokine Production and Does Not Worsen the Cardiac Function and Oxidative State of Diabetic Rats

Antioxidants ◽

10.3390/antiox8120607 ◽

2019 ◽

Vol 8 (12) ◽

pp. 607 ◽

Cited By ~ 2

Author(s):

Rita Benkő ◽

Zsuzsanna Miklós ◽

Viktor Antal Ágoston ◽

Katrine Ihonvien ◽

Csaba Répás ◽

...

Keyword(s):

Oxidative Stress ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Inflammatory Cytokine ◽

Oxygen Therapy ◽

Treatment Protocol ◽

Soft Tissue Injuries ◽

Diabetic Rats ◽

Aortic Tissue ◽

Diabetic Patients

Hyperbaric oxygen therapy (HBOT) is frequently used after soft tissue injuries and in diabetic patients with ulcerated wounds; however, its ability to increase oxidative stress casts doubts. Diabetes (DM) in male Wistar rats (N = 20) weighing 300 g were induced by a single dose of streptozotocin. Ten diabetics (DMHBOT) and 10 controls (CHBOT) underwent a one-hour long hyperbaric oxygen treatment protocol (2.5 bar) 12 times after the 3rd week of diabetes. Ten animals remained untreated. Eight weeks after diabetes induction, we measured the 24-hour blood glucose profile and cardiovascular function (sonocardiography and the relaxation ability of aortae). Malonyl-dialdehyde (MDA) and cytokine levels were measured in blood plasma. Poly(ADP-ribose) polymerase (PARP) activity was estimated in cardiac and aortic tissue. HBOT did not alter most of the cardiovascular parameters. PARylation in cardiac and aortic tissues, plasma MDA levels were elevated in diabetic rats. HBOT prevented the increase of MDA in diabetic animals. In addition, levels of the pro-inflammatory cytokine-induced neutrophil chemoattractant-1 (CINC-1) the levels of anti-inflammatory tissue inhibitor of metalloproteases-1 were not altered in diabetes or in hyperoxia. Our results suggest that HBOT does not increase long-term oxidative stress, and, similar to training, the TBARS products, nitrotyrosine formation and poly(ADP-ribosyl)ation may be eased as a result of hyperoxia.

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Prophylactic Use of Troxerutin Can Delay the Development of Diabetic Cognitive Dysfunction and Improve the Expression of Nrf2 in the Hippocampus on STZ Diabetic Rats

Behavioural Neurology ◽

10.1155/2018/8678539 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Songyun Zhang ◽

Lingling Yuan ◽

Lihui Zhang ◽

Caige Li ◽

Jie Li

Keyword(s):

Learning And Memory ◽

Cognitive Dysfunction ◽

Diabetic Rats ◽

Expression Level ◽

Morris Water Maze Test ◽

Control Group ◽

Diabetic Patients ◽

Related Factor ◽

Mda Content ◽

Prophylactic Use

Background. With the change in lifestyle and the aging population, the incidence of cognitive dysfunction in diabetes mellitus is rising sharply. Oxidative stress is an important mechanism in the development of diabetic cognitive dysfunction. Nuclear factor E2-related factor 2 (Nrf2) is the core transcription factor of antioxidative stress. Early prevention and treatment of diabetic cognitive dysfunction can reduce the incidence of dementia and improve the quality of life of diabetic patients. Aim. This study was aimed at determining effect of troxerutin on the development of cognitive dysfunction and the expression level of Nrf2 in the hippocampus of streptozotocin (STZ) diabetic rats, when used in the early preventive stage. Methods. An STZ-induced diabetic rat model was established (n=30), and the animals were randomly divided into 2 groups: diabetic control group (DC, n=15) and diabetic troxerutin intervention group (DT, n=15). Another 10 normoglycemic rats were put into a normal control group (NC, n=10). While the DT group was injected with troxerutin (60 mg/kg), the DC group and the NC group were injected with physiological saline for 12 weeks daily. Learning and memory behaviors were tested using the Morris water maze test. The superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, mRNA level, and protein level of Nrf2 were measured. Data were collected and analyzed by the statistical software package SPSS 19.0, which included one-way analysis of variance with completely randomized design. Results. Learning and memory levels were significantly improved in the DT group compared with the DC group. Moreover, in the DT group, the expression level of Nrf2 in the hippocampus was increased, activity of SOD was elevated, and MDA content was decreased. Conclusion. Prophylactic use of troxerutin delays the development of diabetic cognitive dysfunction and increases the expression level of Nrf2 in the hippocampus of STZ diabetic rats.

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