scholarly journals Etude De Quelques Proprietes Biologiques De Ocimum Gratissimum L., Une Lamiaceae Recoltee A Daloa (Côte d'Ivoire)

2018 ◽  
Vol 14 (3) ◽  
pp. 477
Author(s):  
Elisée Kporou Kouassi ◽  
Sitapha Ouattara ◽  
Cendrine Seguin ◽  
Sylvie Fournel ◽  
Benoit Frisch
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Antitumor Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Colorimetric Assay ◽  
Biological Properties ◽  
Test Results ◽  
Ocimum Gratissimum ◽  
T Lymphoma

Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therapeutic virtues. Scope: Evaluate the biological properties of the essential oil of this plant. Methods: Antitumor activity was assessed using the MTS colorimetric assay on six (06) cell lines, the antifungal activity was evaluated by double dilution slant method on four (04) strains of clinical fungi, the potential antioxidant was determined by the DPPH test. Results: The strongest cytotoxic activity was obtained on Jurkat (T lymphoma) with an IC50 = 80 μg / ml. The best antifungal activity was observed on C. albicans with MIC and FMC values obtained at 12.5 μg / ml. Antioxidant activity was evaluated at F = 187 ± 1.57 mM Trolox / ml or (I = 38 ± 0.74%). Conclusion: The essential oil has interesting biological properties like as cytotoxic, antifungal and antioxidant

The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

10.5219/1695 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1112-1119
Author(s):  
Hana Ďúranová ◽  
Veronika Valková ◽  
Lucia Galovičová ◽  
Jana Štefániková ◽  
Miroslava Kačániová
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Vapor Phase ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Wheat Bread ◽  
Citrus Reticulata ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF THE ESSENTIAL OIL OF CUMINUM CYMINUM SEEDS

AGROFOR ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Amina BESSEDIK
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Biological Properties ◽  
Bioactive Molecules ◽  
Natural Food ◽  
Cuminum Cyminum ◽  
Research Fields ◽  
Methanolic Extracts

Cuminum cyminum (in arabic: Kemoun) is an important source of therapeutic, cosmetic, bio-food and technological agents. Ancient civilizations used this plant for therapeutic purposes. Extracts from this plant are nowadays strongly used in the industrial and research fields, particularly for the medicinal, pharmacological and cosmetological purposes. Bioactive molecules extracted from Cuminum cyminum may express biocidal activities and prove to be good candidates for new antioxidants. The objective of the present work is to evaluate the biological properties of this plant, including antibacterial and antioxidant effects. The seeds of cumin are harvested at the wilaya of Biskra, and stored in a dry place until their use. They were identified at Hassiba Benbouali University of Chlef. Essential oil is obtained by hydro-distillation using a Clivenger type device (AFNOR, 2000). The obtained sample is stored in sealed bottles at low temperature (4°C) and away from light. The antibacterial effect is assessed by the disc method. The minimum inhibitory concentration (MIC) is determined by standard methods. The antioxidant activity is evaluated by the DPPH free radical trapping method of the methanolic extracts. The essential oil of C. cyminum shows a better activity against Gram+ strains compared to Gram- strains. The determination of MICs leads to the conclusion that its activity can be triggered at a very low concentration. The reaction with DPPH gives an interesting IC50. This testifies to the ability of this essential oil to reduce free radicals. Thanks to the antibacterial activities that we have highlighted, cumin can be considered as a palliative that could replace certain antibiotics. Moreover, its antioxidant activity allows us to use it as a natural food additive.

scholarly journals Thymol Chemotype Origanum vulgare L. Essential Oil as a Potential Selective Bio-Based Herbicide on Monocot Plant Species

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 595 ◽  
Author(s):  
Daniela Gruľová ◽  
Lucia Caputo ◽  
Hazem S. Elshafie ◽  
Beáta Baranová ◽  
Laura De Martino ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Plant Species ◽  
Pseudomonas Syringae ◽  
Penicillium Expansum ◽  
Chemical Pollution ◽  
Origanum Vulgare ◽  
Promising Source ◽  
Dicot Species

Searching for new bio-based herbicides is crucial for decreasing chemical pollution, protecting the environment, and sustaining biodiversity. Origanum vulgare is considered a promising source of essential oil with herbicidal effect. The mode of action is not known. The present study focused on (1) comparison of phytotoxic activity of Origanum vulgare EO on monocot (Triticum aestivum and Hordeum vulgare) and dicot species (Lepidium sativum and Sinapis alba); (2) and evaluating other antimicrobial biological activities against phytopatogen bacteria (Clavibacter michiganensis, Pseudomonas syringae pv. phaseolicola, Pseudomonas savastanoi, and Xanthomonas campestris); antifungal activity against Monilinia fructicola, Aspergillus niger, Penicillium expansum, and Botrytis cinerea; cytotoxic activity and antioxidant activity. According to the GC/MS analyses, the EO belongs to the thymol chemotype O. vulgare with its high content of thymol (76%). Germination of all four species was not influenced by EO. The phytotoxic effect was statistically significant in the monocot species, while in the dicot species the opposite was observed—a stimulation effect, which was also statistically significant. Strong biological activity of O. vulgare EO was noted on all phytopatogen bacteria and fungi in the highest dose. Cytotoxic activity showed an IC50 = 50.5 μg/mL. Antioxidant activity showed an IC50 = 106.6 μg/mL after 45 min experimental time. Based on the presented results, it is possible to conclude that thymol chemotype O. vulgare essential oil could be potentially used as a herbicide with selective effects on monocot plant species.

scholarly journals Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity

2020 ◽  
Vol 13 (12) ◽  
pp. 479
Author(s):  
Márcio Rodrigues ◽  
Ana Clara Lopes ◽  
Filipa Vaz ◽  
Melanie Filipe ◽  
Gilberto Alves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Scientific Information ◽  
Biological Properties ◽  
Fusarium Spp ◽  
Candida Spp ◽  
Pharmaceutical Industries

Thymus mastichina has the appearance of a semishrub and can be found in jungles and rocky lands of the Iberian Peninsula. This work aimed to review and gather available scientific information on the composition and biological properties of T. mastichina. The main constituents of T. mastichina essential oil are 1,8-cineole (or eucalyptol) and linalool, while the extracts are characterized by the presence of flavonoids, phenolic acids, and terpenes. The essential oil and extracts of T. mastichina have demonstrated a wide diversity of biological activities. They showed antibacterial activity against several bacteria such as Escherichia coli, Proteus mirabilis, Salmonella subsp., methicillin-resistant and methicillin-sensitive Staphylococcus aureus, Listeria monocytogenes EGD, Bacillus cereus, and Pseudomonas, among others, and antifungal activity against Candida spp. and Fusarium spp. Additionally, it has antioxidant activity, which has been evaluated through different methods. Furthermore, other activities have also been studied, such as anticancer, antiviral, insecticidal, repellent, anti-Alzheimer, and anti-inflammatory activity. In conclusion, considering the biological activities reported for the essential oil and extracts of T. mastichina, its potential as a preservative agent could be explored to be used in the food, cosmetic, or pharmaceutical industries.

scholarly journals Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Lethicia B. Brandão ◽  
Lizandra L. Santos ◽  
Rosany L. Martins ◽  
Alex B. L. Rodrigues ◽  
Erica de M. Rabelo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Cytotoxic Activity ◽  
Artemia Salina ◽  
Stage Of Development ◽  
Tridax Procumbens ◽  
Third Stage ◽  
Low Toxicity ◽  
Larvicidal Bioassay

The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of Tridax procumbens leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using Artemia salina, the larvicidal bioassay was performed with larvae in the third stage of development of the Aedes aegypti mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and γ-terpinene being the main ones. It presented antioxidant activity with an IC50 of 194.51 μg mL−1, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against A. salina, with an LC50 of 1238.67 μg mL−1, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC50 = 79.0 μg mL−1 in 24 hours and LC50 of 69.15 μg mL−1 in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of T. procumbens presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to A. salina.

scholarly journals Antifungal Activity of the Essential Oils from Ocimum gratissimum L. Grown in Togo

2008 ◽  
Vol 1 (1) ◽  
pp. 164-171 ◽  
Author(s):  
Koffi Koba ◽  
P W Poutouli ◽  
Christine Raynaud ◽  
Komla Sanda
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Filamentous Fungi ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Minimum Fungicidal Concentration ◽  
Ocimum Gratissimum ◽  
Aerial Parts ◽  
In Vitro Antifungal Activity

The aerial parts of Ocimum gratissimum L. (Lamiaceae) harvested in Togo was steam-distilled and investigated for essential oil composition (GC and GC/MS) and in vitro antifungal activities. Thymol (31.79 %), p-cymene (15.57 %) and γ-terpinene (12.34 %) and were the major components of the oil. Other notable components identified in this oil were myrcene (6.94 %) and α-thujene (6.11 %).The in vitro antifungal activity was recorded with the minimum inhibitory concentrations (MICs) ranging from 80 to 150 µl.l-1, 150 to 500 µl.l-1  and from 100 to 150 µl.l-1 respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Likewise, on tested fungi the minimum fungicidal concentration (MFC) varied from 300 µl.l-1 to 500 µl.l-1, 500 to 700 µl.l-1 and from 250 to 300 µl.l-1, respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Keywords: O.gratissimum,  Antifungal, Essential oil; Thymol. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1131 

scholarly journals PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL STUDY OF CAPPARIS CARTILAGINEA DECNEFROM YEMEN

2018 ◽  
Vol 10 (6) ◽  
pp. 38
Author(s):  
Bushra Abdulkarim Moharram ◽  
Hassan M. Al-mahbashi ◽  
Riyadh Saif Ali ◽  
Faten Ali Aqlan
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Human Lung ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Paw Edema ◽  
Anti Inflammatory

Objective: To investigate phytochemicals and biological activities of Capparis cartilaginea extracts.Methods: The methanolic extracts of leaves, stem and twigs of C. cartilaginea were screened for their phytochemicals. The essential oil of the leaves was hydrodistilled by a Clevenger apparatus and analyzed by gas chromatography-mass spectrometry (GC-MS). The leaves extract of C. cartilaginea was evaluated for its anti-inflammatory effect, using formalin-induced paw edema. The leaves, stem and twig extracts were assessed for their antioxidant activity, using free radical scavenging assay, cytotoxic activity, using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and antibacterial activity, using the microdilution method.Results: All extracts of C. cartilaginea contained alkaloids, carbohydrates, protein, coumarin, phytosterols, bitter principles, phenols and tannins. The essential oil of the leaves was mainly composed of isopropyl isothiocyanate (69.4%), butane,1-isothiocyanate (26.97%) and isobutyl isothiocyanate (3.26%). The leaves extract at doses of 200 and 400 mg/kg, significantly inhibited paw edema at the 3rd h (49.1%, 54.0%, respectively) and this effect was comparable to that of diclofenac (58.87%). The leaves extract showed the highest antioxidant activity with IC50 value of 91.71 µg/ml. The twigs extract exhibited the highest cytotoxic activity against human lung carcinoma (A549) with IC50 of 57.5 µg/ml. The leaves and stem extracts exhibited antibacterial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 5.0 mg/ml. Conclusion: The leaves extract of C. cartilaginea is a potential source of bioactive compounds that could have a role in anti-inflammation. Twigs extract of the C. cartilaginea possesses a potential cytotoxic effect on human lung cell line.

scholarly journals An in Vitro Study on Antifungal Properties, Total Polyphenolic Content and Antioxidant Activity of Different Parts of Selected Fruit Trees

2020 ◽  
Vol 71 (4) ◽  
pp. 355-363
Author(s):  
Derya Ustaömer ◽  
Elif Topaloğlu ◽  
Bilge Yilmaz ◽  
Hüseyin Serencam ◽  
İlhan Deniz
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Water Extract ◽  
Fruit Trees ◽  
Hot Water ◽  
Methanol Extracts ◽  
Hot Water Extract ◽  
Polyphenolic Content ◽  
Total Polyphenolic Content

The purpose of this study was to investigate the antifungal properties, total polyphenolic content and antioxidant activity of sapwood, heartwood and bark parts obtained from three different fruit trees; olive (Olea europaea L.), loquat (Eriobotrya japonica Lindl.) and date plum (Diospyros lotus L.) grown in Turkey. Firstly, the hydrodistillation products (essential oil, hydrosol, hot-water extract) were acquired from these parts of selected trees, and methanol and ethanol extracts of the same samples were also prepared for the analysis. The antifungal activities of essential oil, hydrosol, hot-water extract, ethanol and methanol extracts were determined against wood-rot fungus Coniophora puteana. In addition, polyphenol contents and antioxidant activities of ethanol and methanol extracts were investigated. According to the results, generally, the essential oil of the parts of three fruit trees was found to inhibit the fungal growth. All hot water extracts and hydrosols of these trees showed no antifungal activity against C. puteana. It was found that olive tree had higher antifungal activity than loquat and date plum trees. The methanol extract of loquat bark gave the highest total phenolic content, total flavonoid content and condensed tannin content compared to parts of the other trees. Furthermore, the highest total polyphenol content and antioxidant activity among three fruit trees were determined in ethanol and methanol extracts of the bark and heartwood of loquat tree. Based on these findings, it can be concluded that the parts of studied fruit trees have less or more antifungal and antioxidant activity depending on the experimental parameters, and therefore they can be evaluated as alternative natural antifungal and antioxidant sources.

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