scholarly journals Phenolic Content and Antimicrobial activities of Date Palm (Phoenix dactylifera L.) Fruits and Leaves

Food Biology ◽  
2017 ◽  
Vol 6 ◽  
pp. 11 ◽  
Author(s):  
Hajira A. Qadoos ◽  
Hend S. Dhafari ◽  
Dalal A. Al Marzooqi ◽  
Al yzyah I. Yaqoubi ◽  
Alagappan Kumarappan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Date Palm ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Bacterial Species ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Palm Fruit

<p>This study aimed to investigate total phenolic content (TPC) and antibacterial activities of different extracts recovered from Date Palm fruit and leaves using various solvents, including methanol, ethanol and water. The results showed that the highest phenolic content was found in Leaf Methanol extract, followed by Leaf Ethanol extract, and then followed by Seed Methanol extract (74.4 mg/g, 67.3 mg/g, and 64.7 mg/g respectively). Whereas, the date methanol extract had the lowest phenolic content (5 mg/g). The study was also scrutinized to find the antibacterial inhibitory property in the leaves and fruits of date palm against four bacterial species (<em>E.coli</em>, <em>Staphylococcus aureus</em>, <em>Bacillus subtilis</em>, and <em>Pseudomonas aeruginosa</em>) by using the well diffusion method and minimum inhibitory concentration (MIC). Date leaves extracts showed inhibitory effect on the growth of <em>Staphylococcus aureu</em>s and <em>Bacillus subtilis</em> and resistance to <em>Pseudomonas aeruginosa</em> and <em>E.coli</em>. MIC of methanol and ethanol (70%) leaves extract were 100 mg/ml and 250mg/ml for <em>S. aureus</em> and <em>B. subtilis</em> respectively, however, all the bacteria have resistance to date fruits extracts.</p>

Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

2021 ◽  
Vol 2 (1) ◽  
pp. 23-33
Author(s):  
Abdul Aziz ◽  
Veggy Nadya Yuliawan ◽  
Paula Mariana Kustiawan
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Polar Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Content ◽  
Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2  mm ± 1.5) at  200µg/mL concentration.

scholarly journals In-Vitro Studies on Antitumour and Antimicrobial Activities of Methanolic Kernel Extract of Mangifera Indica L. Cultivar Banganapalli

2019 ◽  
Vol 12 (1) ◽  
pp. 357-362 ◽  
Author(s):  
N. Venkata Raju ◽  
Karuganti Sukumar ◽  
G. Babul Reddy ◽  
P. K. Pankaj ◽  
Muralitharan G. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Mangifera Indica ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Mangifera indica L. belongs to the family Anacardiaceae and is considered as “King of all Fruits”. Mango kernels are discarded as waste after the industrial processing and it has several proven medicinal benefits. Attempts were made to study its antitumour and antimicrobial activities. In the current research work, 15 local cultivars of Mangifera indica L. were collected with a motto to screen the best cultivar having high total phenolic content, flavonoid content, antitumour property and antimicrobial activity. Banganapalli cultivar of mango showed high total phenolic content and total flavonoid content i.e. 63.5±1.1 mg GAE/g and 16.7±0.5 mg quercetin/g followed by Royal special cultivar (TPC-58.7±0.6 mg GAE/g TFC-16.2±0.6 mg quercetin/g). Mangifera indica L. cultivar Banganapalli which showed highest total phenolic content and total flavonoid content was screened for its antitumour and antimicrobial properties. Antitumour property was tested by using potato disc assay which recorded 40.12% tumour inhibition percentage. Antimicrobial activity was assessed by agar diffusion method by taking 3 test microorganisms viz. Bacillus subtilis subsp. subtilis DSM 10, Staphylococcus aureus MTCC 737 and Escherichia coli MTCC 46. The measured area of inhibition is around Staphylococcus aureus MTCC 737 in 8.5±0.3 mm followed by E.coli MTCC 46 (8.2±0.3 mm) and Bacillus subtilis sub subtilis (6.6±0.5 mm). The present study showed that the mango kernels which were generally discarded as waste has antitumour and antibacterial properties and further studies need to be carried out.

scholarly journals Antioxidant and Antibacterial Activity of Methanol Extract of Momordica Balsamina

2018 ◽  
Vol 10 (2) ◽  
pp. 7
Author(s):  
Sajini Souda ◽  
Saramma George ◽  
Naledi Mannathoko ◽  
Irene Goercke ◽  
Kelvin Chabaesele
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Zones Of Inhibition ◽  
Gram Negative Organisms

<em>Momordica balsamina </em>is considered as a miracle herb used in African traditional medicine due to its tremendous medicinal and nutritional properties. In this present study we evaluated the antioxidant and antimicrobial activities of the methanol extract of <em>Momordica balsamina </em>(MEMB). Phytochemical screening followed by thin layer chromatography and spectrophotometrydone to measure the radical scavenging activity of 2,2-diphenyl-l-picrylhydrazyl (DPPH). Total phenolic content and total antioxidant activity of MEMB was determined. Zones of inhibition, minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) were measured by agar diffusion and liquid broth dilution assays to assess the antimicrobial activities of MEMB. Phytochemicals isolated were flavonoids, tannins, coumarins, terpenoids and phenols. The antioxidant potential and antimicrobial activity of MEMB can be attributed to the total phenolic content and other bioactive phytochemicals.MEMB showed antimicrobicidal activity more against Gram positive than Gram negative organisms. <em>S.agalactiae,S.aureus</em> (ATCC 25923) and <em>L.monocytogenes</em> were more susceptible than <em>Proteus mirabilis</em>, <em>E.coli, K.pneumoniae, P.aeruginosa or S. typhimurium. </em>MEMB also showed antifungal activity against <em>C.albicans</em> species though at a higher concentration[MIC - 0.938mg/ml and MBC-1.875mg/ml]. MEMB can therefore be considered as a potential medication in the management of infectious diseases.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

scholarly journals Total Phenolic Content of Methanol Extract from Buni Fruits (Antidesma bunius L.) Water

2020 ◽  
Vol 1655 ◽  
pp. 012029
Author(s):  
M. Yasser ◽  
Mohamad Rafi ◽  
Wulan Tri Wahyuni ◽  
Andi Muhamad Iqbal Akbar Asfar ◽  
Setyo Erna Widiyanti
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic

scholarly journals Evaluation of Bioactivities of Gouania tiliaefolia Lam., an Indigenous Traditional Medicinal Plant of Bangladesh

2019 ◽  
Vol 22 (2) ◽  
pp. 200-207
Author(s):  
Tufael Ahmed ◽  
Rifat Khan ◽  
Nafisa Tabassum ◽  
Fahima Aktar ◽  
Mohammad Kaisarul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Crude Methanol Extract

The crude methanol extract of Gouania tiliaefolia Lam. was partitioned by the modified Kupchan method and the fractions were evaluated for total phenolic content, antioxidant, cytotoxic, thrombolytic, hypotonic and heat-induced membrane stabilizing activities. The petroleum ether soluble fraction (PESF) and methanol extract (ME) showed the highest phenolic content of 78.30 ±1.60 mg and 70.37 ± 0.84 mg, respectively, which were expressed in gallic acid equivalent (GAE). Similar trends were observed in case of anti-oxidant and cytotoxic activities, where the PESF possessed the highest free radical scavenging activity and brine shrimp lethality (IC50 = 2.88 ± 0.02 μg/ml, LC50 = 2.59 ± 0.14 μg/ml), followed by ME (IC50 = 4.79 ± 0.17 μg/ml, LC50 = 3.38 ± 0.08 μg/ml) and CSF (IC50 = 37.51 ± 0.96 μg/ml, LC50 = 73.55 ± 0.26 μg/ml). In case of assays for thrombolytic and membrane stabilizing activities, all extractives showed insignificant results compared to the respective standards. The crude methanol extract of G. tiliaefolia was used to examine the in-vivo analgesic (central and peripheral), antidiarrheal and antidiabetic activities in Swiss albino mice. In case of castor oil induced diarrhea, the ME gave better reduction of diarrhea by 71.43% (at 400 mg/kg-body weight) compared to loperamide (64.29%). Antidiabetic activity was evaluated by oral glucose tolerance test and the ME showed 71.42% and 75.39% reduction of blood glucose at doses 200 and 400 mg/kg-body weight, respectively when compared with the standard glibenclamide that reduced blood glucose by 66.17%. The central- and peripheral-analgesic activity was evaluated by the tail-flick test and acetic acid induced writhing test, respectively. In both the cases, ME demonstrated dose-dependent analgesic activity compared to the standards. Bangladesh Pharmaceutical Journal 22(2): 200-207, 2019

Evaluation of Solvent Extraction Process for Asparagus racemosus Root Extract through the Determination of its Phenolic Content and Antioxidant Activity Assay

2021 ◽  
pp. 5108-5114
Author(s):  
Monika D. Bhalodiya ◽  
Jayant R. Chavda ◽  
Nilesh K Patel ◽  
Ajay I. Patel ◽  
Ravi A. Manek
Keyword(s):  
Methanol Extract ◽  
Phenolic Content ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Asparagus Racemosus ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Aqueous Methanol ◽  
Antioxidant Assay ◽  
Saponin Content

This study is aimed to evaluate extraction capacity of different solvents through determining total phenolic content, total flavonoid content, total saponin content, and an antioxidant assay of different solvent extracts (toluene, dichloromethane, chloroform, ethyl acetate, ethanol, methanol, water, 60% aqueous methanol, 60% aqueous ethanol and 60% aqueous acetone) of Asparagus racemosus (A. racemosus) root. The 60% aqueous methanol shows the highest extracting yield, in contrast, toluene shows the lowest yield. Highest total phenolic content (220.8±0.74mg GAE/g), total flavonoid content (219.3±0.64mg QCE/g), total tannin content (108.4±0.88mg GAE/g) were found from acetone extract. The methanol extract showed the highest saponin content (579.4±2.85mg ESE/g). Antioxidant potential determines by using various in vitro methodologies such as DPPH, FRAP, and ABTS assay. The lowest IC50 value was found in acetone extract and thus it exhibited the highest antioxidant activity. The highest correlation was found between phenolic content and the antioxidant assay. It seems that phenolic contents are responsible for free radicle scavenging activity. From the observation, it concluded that acetone extract rich with polyphenolic content and methanol extract shows the highest amount of saponin content.

Antimicrobial activities of different parts of two geographically distinct varieties of Jatropha curcas Linn. fruit in Pakistan

2021 ◽  
Vol 50 (2) ◽  
pp. 219-226
Author(s):  
Abdul Haq ◽  
Alam Khan ◽  
Zulfiqar Ali Malik ◽  
Mushtaq Ahmed ◽  
Samiullah Khan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Seed Coat ◽  
Jatropha Curcas ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Seed Kernel ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Antimicrobial activities of deoiled seed kernel (mechanically pressed), fruit coat and seed coat of Jatropha curcas Linn. collected from two regions (Bannu and Peshawar) of Pakistan were investigated. The antimicrobial activities were carried out against Klebsiella pneumoniae (ATCC 43816), Escherichia coli (ATCC 10536), Staphylococcus aureus (ATCC 6538), Bacillus subtilis (ATCC 6633) and two clinical fungal isolates Aspergillus fumigatus and Candida albicans using agar well diffusion method. The antibacterial activities of Peshawar sample were found to be higher than Bannu, against selected strains. While antifungal activities of both samples were similar. Highest zone of inhibition 31.5 ± 0.7 mm was exhibited by n-hexane extract of deoiled seed kernel of Peshawar sample against Bacillus subtilis (ATCC 6633). The minimum inhibitory concentration of ethanolic extracts of deoiled seed kernel and seed coat of Peshawar sample was 31.25 - 25 mg/ml. Whereas, minimum inhibitory concentration of ethanolic and n-hexane extracts of Bannu sample was 62.5 - 125 mg/ml. The results suggested that antimicrobial potential of J. curcas Linn. varied with geographical distribution. The investigation of different varieties of medicinal plants belonging to the same species will greatly enhance the chances of best pharmaceuticals discovery. Bangladesh J. Bot. 50(2): 219-226, 2021 (June)

scholarly journals Screening Of Antibacterial Activity Of Nepeta Ciliaris Benth. Against Respiratory Tract Pathogens

1970 ◽  
Vol 8 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Gautam Shiv Shankar ◽  
M Navneet ◽  
Kumar Sanjay ◽  
M Prabhat
Keyword(s):  
Respiratory Tract ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Bacterial Species ◽  
Positive Control ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Antibacterial Potential

The aim of present study was to evaluate the antibacterial potential of various extracts (petroleum ether, acetone, methanol and aqueous) of Nepeta ciliaris against selected respiratory tract pathogens. The extracts from the aerial parts of N. ciliaris at concentration of 200 mg/ml were screened against three gram-positive (Staphylococcus aureus MTCC 1144, Streptococcus pneumoniae MTCC 655 and Streptococcus pyogenes MTCC 442) and one gram-negative (Pseudomonas aeruginosa MTCC 2474) bacterial pathogens. The agar well diffusion method was adopted to examine antibacterial and minimum inhibitory concentration (MIC) values of most effective extracts against the susceptible bacteria. Erythromycin was used as positive control to determine the sensitivity of the strains. Out of the four bacterial species tested, S. pneumoniae was the most susceptible. The acetone extract exhibited maximum activity against all the tested microorganisms while methanol extract showed activity against P. aeruginosa. The MIC values ranged from 40 to 50 mg/ml for all the organisms. The N. ciliaris is potentially a good source of antimicrobial agents. DOI: http://dx.doi.org/10.3126/kuset.v8i1.6049 KUSET 2012; 8(1): 100-103

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

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