scholarly journals ¿Qué hay ahí? Similitudes y diferencias de la microbiota del alga roja Chondracanthus chamissoi de Ancón y Paracas

2020 ◽  
Author(s):  
◽  
Eiji Leandro Pereira Gil ◽  
◽  
Flor de Rosa María Vigo Saavedra
Keyword(s):  
16S Rrna ◽  
Base Space ◽  
Chondracanthus Chamissoi ◽  
Omega 6 ◽  
Seriola Lalandi ◽  
Pseudoalteromonas Rubra

La gran actividad metabólica que caracteriza la microbiota permite mantener una relación de simbiosis con el ser humano, impactando de forma positiva en el desarrollo vital de ambos. Algunos alimentos aportan a la microbiota, entre estos se encuentran los fermentados, verduras, frutas y algas. La biota de diferentes algas ha sido tipificada, sin embargo no se habían realizado estudios del alga roja más consumida en Perú, Chondracanthus chamissoi (Yuyo). En este estudio se tipificó la microbiota de C. chamissoi Ancón y Paracas, las cuales fueron extraídas del mar, compradas del mercado del lugar, además se muestreó agua de mar de Ancón (Lima) y Paracas (Ica) mediante secuenciación de nueva generación (NGS, por sus siglas en inglés) del gen 16S rRNA. Como resultado se obtuvo un total de 153 323 secuencias, las cuales fueron asignadas taxonómicamente en 19 filos, 41 clases, 90 órdenes, 193 familias, 484 géneros y 716 especies mediante la plataforma Base Space de Illumina. El filo más encontrado fue Proteobacterias seguido por Bacteroidetes y Cyanobacterias. Se encontraron 24 especies en común entre las muestras de alga. Además, se encontró un número elevado de especies presentes solo en muestras extraídas de los mercados correspondientes, indicando posible contaminación cruzada. Entre el 55,27%-58,87% de especies bacterianas encontradas resultaron no estar clasificadas. El análisis semi-cuantitativo permitió a su vez asignar la representación relativa de cada bacteria en las algas de Ancón y Paracas. Muchas especies que se encontraron en una abundancia significativa han sido previamente estudiadas, muchas de ellas implicadas en la industria alimentaria y/o salud de humanos y animales. Este es el caso de Psycrobacter namhaensis; empleada como probiótico para la tilapia del Nilo de manera exitosa. Lewinella marina es capaz de utilizar la celulosa como fuente de energía y así degradarla pudiendo ser útil en la industria cervecera. Pseudoalteromonas rubra posee poder probiótico al mejorar el cultivo de las larvas del pez dorado Seriola lalandi y además Loktanella sp. posee ácidos grasos omega 6, 7, 10. En conjunto, el presente estudio describe de manera exhaustiva la riqueza microbiana asociada al yuyo, importante componente de la gastronomía peruana.

scholarly journals Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales)

2012 ◽  
Vol 79 (5) ◽  
pp. 1590-1597 ◽  
Author(s):  
M. C. Stride ◽  
A. Polkinghorne ◽  
T. L. Miller ◽  
J. M. Groff ◽  
S. E. LaPatra ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Arctic Charr ◽  
Rrna Sequence ◽  
Content Type ◽  
New Family ◽  
Gill Lamellae ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi

ABSTRACTThree cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined forChlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the orderChlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “CandidatusPiscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35ChlamydiaandChlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the orderChlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as “CandidatusParilichlamydia carangidicola” and that the new family be known as “CandidatusParilichlamydiaceae.”

Protein-induced conformational changes in 16S rRNA during assembly of the Escherichia Coli 30S ribosomal subunit: A STEM study

1987 ◽  
Vol 45 ◽  
pp. 910-911
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
16S Rrna ◽  
Conformational Changes ◽  
Ribosomal Proteins ◽  
Structural Changes ◽  
Ribosomal Subunit ◽  
Compact Structure ◽  
E Coli ◽  
Freeze Dried ◽  
16S Rna ◽  
30S Subunit

The aim of this study is to understand the mechanism of 16S rRNA folding into the compact structure of the small 30S subunit of E. coli ribosome. The assembly of the 30S E. coli ribosomal subunit is a sequence of specific interactions of 16S rRNA with 21 ribosomal proteins (S1-S21). Using dedicated high resolution STEM we have monitored structural changes induced in 16S rRNA by the proteins S4, S8, S15 and S20 which are involved in the initial steps of 30S subunit assembly. S4 is the first protein to bind directly and stoichiometrically to 16S rRNA. Direct binding also occurs individually between 16S RNA and S8 and S15. However, binding of S20 requires the presence of S4 and S8. The RNA-protein complexes are prepared by the standard reconstitution procedure, dialyzed against 60 mM KCl, 2 mM Mg(OAc)2, 10 mM-Hepes-KOH pH 7.5 (Buffer A), freeze-dried and observed unstained in dark field at -160°.

Imaging of ribosomal RNA-protein interactions by dark-field STEM

1989 ◽  
Vol 47 ◽  
pp. 250-251
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
S. Tumminia ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
Nucleic Acid ◽  
16S Rrna ◽  
Dark Field ◽  
Radius Of Gyration ◽  
Central Domain ◽  
The Core ◽  
16S Rna ◽  
30S Subunit ◽  
Core Particles

Success in protein-free deposition of native nucleic acid molecules from solutions of selected ionic conditions prompted attempts for high resolution imaging of nucleic acid interactions with proteins, not attainable by conventional EM. Since the nucleic acid molecules can be visualized in the dark-field STEM mode without contrasting by heavy atoms, the established linearity between scattering cross-section and molecular weight can be applied to the determination of their molecular mass (M) linear density (M/L), mass distribution and radius of gyration (RG). Determination of these parameters promotes electron microscopic imaging of biological macromolecules by STEM to a quantitative analytical level. This technique is applied to study the mechanism of 16S rRNA folding during the assembly process of the 30S ribosomal subunit of E. coli. The sequential addition of protein S4 which binds to the 5'end of the 16S rRNA and S8 and S15 which bind to the central domain of the molecule leads to a corresponding increase of mass and increased coiling of the 16S rRNA in the core particles. This increased compactness is evident from the decrease in RG values from 114Å to 91Å (in “ribosomal” buffer consisting of 10 mM Hepes pH 7.6, 60 mM KCl, 2 m Mg(OAc)2, 1 mM DTT). The binding of S20, S17 and S7 which interact with the 5'domain, the central domain and the 3'domain, respectively, continues the trend of mass increase. However, the RG values of the core particles exhibit a reverse trend, an increase to 108Å. In addition, the binding of S7 leads to the formation of a globular mass cluster with a diameter of about 115Å and a mass of ∽300 kDa. The rest of the mass, about 330 kDa, remains loosely coiled giving the particle a “medusa-like” appearance. These results provide direct evidence that 16S RNA undergoes significant structural reorganization during the 30S subunit assembly and show that its interactions with the six primary binding proteins are not sufficient for 16S rRNA coiling into particles resembling the native 30S subunit, contrary to what has been reported in the literature.

Assessment of the Dietary Intakes of 6- and 12-Month-Old Infants in Saudi Arabia

2017 ◽  
Vol 87 (1-2) ◽  
pp. 37-48
Author(s):  
Riyadh A. Alzaheb ◽  
Norah Alatawi ◽  
Khawla A. Daoud ◽  
Naema Altawil
Keyword(s):  
Vitamin D ◽  
Saudi Arabia ◽  
Age Groups ◽  
Dietary Intakes ◽  
Dietary Survey ◽  
Daily Energy ◽  
Omega 6 ◽  
24 Hour Recall ◽  
Adequate Nutrient ◽  
Selection Of

Abstract.Background: Establishing understanding of infants’ dietary intakes can support interventions to improve their diets and overall health. Because information on the dietary intakes of infants aged ≤12 months in Saudi Arabia is scarce, this study examined the diets of infants aged 6 and 12 months in Saudi Arabia and determined their main dietary sources of total energy and macronutrients. Methods: A crosssectional dietary survey employing a single 24-hour recall was performed between May and December 2015 with a sample of mothers of 278 healthy 6-month-old and 259 12-month-old infants. An analysis of the dietary intake data determined the nutrient intake adequacy and the percentage contributions of foods to energy and macronutrient intakes. Results: The respective mean daily energy intakes of the 6-month-old and 12-month-old infants in the study were 703 kcal and 929 kcal. Both age groups recorded adequate nutrient intakes, with the exception that the 6-month-olds’ mean vitamin D intake fell below the recommended Adequate Intake (AI), and the 12-month-olds’ intakes of omega-6 fatty acids and vitamin D were also below the AI, along with their iron intake which fell short of the Recommended Dietary Allowance (RDA). Conclusion: The data generated here will assist health professionals in planning interventions which aim to improve infants’ diets and to offer guidance to parents on the appropriate selection of food for their infants.

Peran DHA dalam Pencegahan Preeklampsia

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Herlambang
Keyword(s):  
Docosahexaenoic Acid ◽  
Omega 6

AbstrakDiet maternal diduga berperan dalam etiologi preeclampsia termasuk salahsatunya asam lemak tidak jenuh rantai panjang. Asam lemak tersebut dapat berupaomega-3 dan omega-6 yang dikonversikan menjadi AA dan DHA di dalam tubuhmanusia. AA (Asam Arakhidonat) dan DHA (Docosahexaenoic Acid) merupakankomponen penting dari fosfolipid membrane yang berperan dalam meregulasi fungsimembrane sel dan dapat mencegah preeclampsia dengan memodulasi inflamasi danfungsi vaskular yaitu mengurangi kadar tromboksan (TAX2) dan meningkatkanprostasiklin (PGI2) pada tubuh maternal.

Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

2015 ◽  
Vol 41 (1) ◽  
pp. 51-58
Author(s):  
Mohammad Shamimul Alam ◽  
Hawa Jahan ◽  
Rowshan Ara Begum ◽  
Reza M Shahjahan
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Fish Larva ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Valuable Insight ◽  
Multiple Sequence ◽  
Pcr Rflp ◽  
Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

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