scholarly journals The Effectiveness of 2.5% NaOCl Irrigation and 17% EDTA against the Sealing Ability of Resin Paste

2020 ◽  
Vol 9 (2) ◽  
pp. 105
Author(s):  
Tamara Nitya A ◽  
Nanik Zubaidah ◽  
Moch Mudjiono

Background: Root canal irrigation seeks the elimination of bacteria and its products from the root canal. If debris is left in the root canal, it can prohibit adaptation between obturationmaterial and root canal wall. Therefore, it can decrease the sealing ability of obturation material. Purpose: To assess the effectiveness of 2.5% NaOCl and 17% EDTA on the apical sealing ability of resin obturation paste. Method: The roots of 18 human mandibular premolar teeth were selected and the root canal was prepared using a conventional technique. Samples were irrigated with 2.5% NaOCl, and17% EDTA, and sterile aquadest (Aqua Deastilata) as a control group. Samples were filled with gutta-percha and top seal. Samples were soaked in 2% methylene blue for 48 hours to measure the depth of microleakage. Result: The mean apical leakage after irrigation with 2.5% NaOCl; 17% EDTA; and sterile aquadest in the sequence were 0.92 mm; 3.6 mm; and 8.08 mm. Conclusion: 2.5% NaOClhas less depth of microleakage than 17% EDTA. However, the depth of microleakage cannot demonstrate the exact sealing ability of the resin paste due to some factors.

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Soo-Hyuk Lee ◽  
Soram Oh ◽  
Adel Saeed Al-Ghamdi ◽  
Ayman Omar Mandorah ◽  
Kee-Yeon Kum ◽  
...  

The objective of root canal obturation is to achieve a fluid-tight seal. Recently, GuttaFlow bioseal (GB), a root canal sealer composed of polydimethylsiloxane, gutta-percha particles, and bioactive glass ceramics, has been developed, to enhance the sealing ability of root canal filling material. The objective of this study was to assess the sealing ability of GB using a subnanoliter-scaled fluid-flow measuring device and to compare with that of AH Plus (AH). The fluid flow in root canal-filled teeth using either gutta-percha cone (GP) with AH (GAR; n = 10) or GP with GB (GBR; n = 10) and in GP inserted in AH blocks (GA; n = 10) or GP inserted in GB blocks (GB; n = 10) was measured. In addition, fluid flow in sealer blocks of AH (A; n = 10) and GB (B; n = 10), which served as negative controls, was measured. Root canal-filled teeth using GP without any sealer (GR) acted as positive controls (n = 10). The leakage was obtained by calculating the volume of moved water by time (s), after stabilization of the fluid flow was achieved. Statistical analysis was performed using the Kruskal–Wallis test and Mann–Whitney U-test with Bonferroni correction. A p value less than 0.00238 (0.05/21) was considered significantly different. The mean leakages (nL/s) in the groups are as follows: GAR, 0.0958 ± 0.0543; GBR, 0.0223 ± 0.0246; GA, 0.0644 ± 0.0803; GB, 0.0267 ± 0.0182; A, 0.0055 ± 0.0057; B, 0.0052 ± 0.005; and GR, 0.2892 ± 0.3018. The mean leakage in the GBR group was lower than that in the GAR group (p = 0.001), while the mean leakages in the GA and GB groups were not significantly different. GuttaFlow bioseal can be useful in single-cone obturation technique.


2019 ◽  
Vol 10 (4) ◽  
pp. 2869-2874
Author(s):  
Dennis D ◽  
Winda Fatika Sari ◽  
Trimurni Abidin ◽  
Widi Prasetia

Factor that should be considered in the use of irrigation is the loss of the smear layer on root canal walls. Therefore the prepared root canal can be irrigated using materials, such as EDTA 17% and 2,5% NaOCl and of extract Acanthus ilicifolius 7,5% and 10%. To determine the potential of extract Acanthus ilicifolius leaves as an alternative of root canal irrigation in removing smear layer at a concentratiom of 7,5% and 10%. The type of this study is a laboratory experimental with post-test only control group design. The total sample is 24 teeth samples that have been prepared and irrigated consisting of four groups. 6 samples for irrigation 7,5% extract Acanthus ilicifolius, 6 samples for irrigation 10% extract Acanthus ilicifolius, 6 positive control samples for combination 2,5% NaOCl and EDTA 17%, and 6 negative control samples. Shooting tool used to observe the cleanliness level in the walls of the root canal is a Scanning Electron Microscope (SEM). Data were analyzed using Kappa statistics, followed by Kruskal wallis analysis test and continued with Mann-Whitney analysis. Acanthus ilicifolius 7.5% and 10% extracts can remove the smear layer when compared with the negative control group. The combination of 2.5% NaOCl and 17% EDTA is better in smear layer removal compared to 7.5% and 10% Acanthus ilicifolius extracts. Acanthus ilicifolius was able to clean the smear layer on the root canal and can be used as an alternative root canal irrigation.


2020 ◽  
Vol 10 (2) ◽  
pp. 44
Author(s):  
Nia Pramais Octaviani ◽  
Latief Mooduto ◽  
Achmad Sudirman

Background: Fusobacterium nucleatum is a common bacterial in root canal with pulp necrosis and periradicular lesion. A way to eliminate these bacteria from root canal is by root canal irrigation. Root canal irrigation materials that are widely used nowadays has many shortcomings. The pericarp of mangosteen (Garcinia mangostana L) has antibacterial potency. Therefore mangosteen pericarp can be an alternative material which could inhibit and bactericidal function to Fusobacterium nucleatum. Purpose: The aim of this study was to determine the antibacterial potency of mangosteen pericarp extract (Garcinia Mangostana L.) against Fusobacterium nucleatum. Methods: This study was laboratory experimental with pos-test only control group design. A microdilution method was used to determine minimum inhibitory concentration and minimum bactericidal concentration by colony counting bacteriae in Tryptone Soya Agar (TSA) media with drop plate technique. Growth of bacterial colonies in TSA is calculated manually in colony forming unit (CFU/ml). Results: Bacterial colonies growth at concentration 0.78% was 90% less than positive control group and there were no bacterial colonies growth at concentration 0.975%. Conclusion: The Minimum Inhibitory Concentration (MIC) of mangosteen pericarp against Fusobacterium nucleatum was at 0,78% concentration and the Minimum Bactericidal Concentration (MBC) was at 0.975% concentration.


Polymers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 3482
Author(s):  
Nidambur Vasudev Ballal ◽  
Amal Roy ◽  
Matthias Zehnder

The conditioning of the root canal wall during chemo-mechanical root canal treatment differentially affects the adhesion of root canal sealers. This investigation evaluated the impact of sodium hypochlorite (NaOCl) concentration as used in a root canal irrigation concept called continuous chelation, with 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) contained in the NaOCl solution that is applied. Fourier-transform infrared spectra of the dentinal wall were gathered. The consequential effects on push-out bond strength of an epoxy resin (AH Plus) versus a hydraulic CaSi sealer (BioRoot RCS) were assessed. Single-rooted extracted human teeth were used and irrigated with pure NaOCl at a concentration of 0% (physiological saline), 2.5%, or 5.25%. Dual Rinse HEDP (9%) was added to the solutions, or not added for further control. Pure NaOCl solutions caused a decrease in the amide III: phosphate ratios, which was counter-acted by the addition of HEDP. It was observed that the adhesion of the epoxy resin sealer under investigation was negatively affected by this NaOCl deproteinization of the canal wall in a dose-dependent manner, while the opposite was observed with the CaSi sealer. HEDP when used in conjunction with NaOCl was beneficial for the adhesion of both sealers.


2019 ◽  
Vol 52 (3) ◽  
pp. 122
Author(s):  
Nirawati Pribadi ◽  
Karlina Samadi ◽  
Meliavita N. K. Astuti ◽  
Hendy J. Kurniawan ◽  
Adelina K. Tandadjaja ◽  
...  

Background: The smear layer is attached to dentine and occludes the orifice and, consequently, must be removed in order to improve the success of inroot canal treatment. The ideal irrigation material removes both the organic and inorganic smear layers. Ethylene diamine tetra-acetic acid (EDTA) is one of the most commonly used root canal irrigation materials, but removes only inorganic smear layer. To overcome this problem, Pineapple (Ananas comosus L. Merr.) peel extract, which contains saponins, bromelain, polyphenol and flavonoid, is used during root canal irrigation. Purpose: The study aimed to analyze the difference in smear layer removal between the use of 6.25% pineapple peel extract and 17% EDTA. Methods: 27 samples of mandibular premolar teeth with straight root canals were divided randomly into three groups (n = 9) and subsequently prepared using protaper. Irrigation was performed on the control group (aquadest), group I (17% EDTA) and group II (6.25% pineapple peel extract). The samples were dried, temporarily compressed and cut horizontally from the apical to the coronal. Samples were fixed with holder before the smear layer was observed through a scanning electron microscope (SEM). The resulting data was analyzed by means of an ANOVA test. Results: The highest score of root canal hygiene was recorded by group II, followed by group I and, finally, the control group. There were significant differences between the groups (p< 0.000). Conclusion: 6.25% pineapple peel extract produces a higher smear layer removal effect than 17%EDTA on the apical 1/3 of the root canal.


2019 ◽  
Vol 8 (1) ◽  
pp. 42
Author(s):  
Tabita Kusumawardhani ◽  
Sukaton Sukaton ◽  
Achmad Sudirman

Background. Bacteria is main etiologycal factor of pulpo-periapical disease. Therefore, eliminating bacteria is the important part on root canal treatment. It can be obtained by using irrigation agent that has antibacterial effect and endodontic sterilization agent. Beside of its antibacterial effect, the ideal irrigation agent should has an ability to remove necrotic tissue and less toxic. Propolis is well known as a natural substain that has antibacterial effect and less toxicity. So, it has a potential use as irrigation agent compared with common root canal irrigation agent, sodium hypochloryte. Purpose. The aim of this study was to observed the difference of antibacterial effect between propolis solution and sodium hypochlorite. Method. Nine samples of Streptococcus viridans were prepared and each sample devided into three parts namely group P (propolis solution), group N (sodium hypochlorite), and control group S (saline). Each group was treated with 0,01 cc agent of root canal irrigation, then was stored in incubator 370C for 24 hours. After that, inhibition zone diameter of  each group was observed.Result. The average of inhibition zone diameters in sodium hypochlorite’s group was 23,47 mm; in propolis solution’s group was 18,70 mm. There were significant difference of antibacterial effect between propolis solution and sodium hypochlorite, against Streptococcus viridans.  Conclussion. Antibacterial effect of sodium hypochlorite is higher than propolis solution, but we should consider the potential use of natural agent-propolis solution as root canal irrigation due to its antibacterial effect.


2016 ◽  
Vol 6 (2) ◽  
pp. 93
Author(s):  
Ranggi Hardian Nugro Astuti ◽  
Karlina Samadi ◽  
Eric Priyo Prasetyo

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml  Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.


2021 ◽  
Vol 6 (2) ◽  
pp. 201
Author(s):  
Sherli Diana ◽  
Rizki Sri Yuliati ◽  
Bayu Indra Sukmana

Background: The success or failure of root canal treatment is influenced by anatomical factors of the root canal, bacterial infection that causes inadequate hygiene, formation and filling of root canals, iatrogenic factors and several other factors. The average success of root canal treatment is 86-95%, while the failure of canal treatment ranges from 5-14%. Root canal re-treatment can be performed in the event of failure of root canal treatment. The method that can be used is gutta percha solvent. The gutta percha solvents used were Chloroform, Eucalyptol, Orange Oil, and Xylene, but chloroform has been categorized as a carcinogenic substance. Guta percha solvent can cause the intercrystalline distance to be larger, so that it can damage the apical density at obturation. This can create a gap between the gutta percha and the sealer which can cause apical leakage. Objective: To compare the value of apical leakage after root canal re-treatment between gutta percha solvent eucalyptol and orange oil. Method: The research method used is True Experimental with post-test only with control group design. A minimum sample of 8 teeth in a group. Total samples from all groups were 24 teeth. The teeth were stained using methylene blue and measured using stereomycroscopes. Results: the highest mean apical leakage was eucalyptol (4.55±2.59), xylene (2.77±1.94), and the lowest was citrus oil (2.20 ±1.06). The results of One-Way ANOVA test showed that there were no significant differences between orange oil, eucalyptol, and xylene (positive control). Conclusion: Eucalyptol has a higher average apical leakage value compared to orange oil and Xylene after root canal re-treatment as a gutta percha solvent. There was no significant difference between orange oil, Eucalyptol, and Xylene.Keywords: Apical leakage, eucalyptol, orange oil


2007 ◽  
Vol 54 (3) ◽  
pp. 160-169
Author(s):  
Goran Stojilkovic ◽  
Jovanka Gasic ◽  
Jelena Dakovic

Introduction: Root canal irrigation plays an important part in the endodontic therapy. Inadequate irrigation or instrumentation without irrigation increase the accumulation of debris on the cutting surfaces of instruments as well as debris and smear layer on root canal walls. Aim: The aim of this study was to evaluate smear layer removal after irrigation with saline, 5% NaOCl, 15% EDTA and the combination of 15% EDTA and 5% NaOCl. Materials and Methods: The study was conducted at the Clinic for Dentistry and Institute for biomedical research of the School of Medicine in Nis. Twenty five premolars, extracted from orthodontic reasons, were allocated to 5 groups (A-E) of 5 teeth. Root canals of all teeth were instrumented using hand K files. Samples from Group A were instrumented without irrigation (control group). In Group B, the irrigation was done using saline, in Group C 5% NaOCl, in Group D 15% EDTA and in Group E the combination of 15% EDTA and 5% NaOCl. After the treatment, all samples were prepared for scanning electron microscopy (SEM). Central parts of root canals were examined and photomicrographs were taken at different magnification. The amount of debris and smear layer was quantified and the data were statistically analyzed. Results: All four treatment modalities (B, C, D, E) induced changes in the appearance of dentinal walls and the amount of debris and the smear layer compared to the control group (A). The difference between the control group and Group B was statistically significant (p<0.01) and highly significant between the control group and Groups C, D and E (p<0.0001). The combined use of 15% EDTA and 5% NaOCl for irrigation and 1 min final rinse resulted in a complete elimination of the smear layer; the use of saline, 5% NaOCl or 15% EDTA alone failed to remove the debris and smear layer completely. Conclusion: The use of singular solutions for root canal irrigation significantly reduces the amount of debris and the smear layer whereas the combined use of NaOCl and EDTA results in the complete elimination of the smear layer from root canal walls.


2015 ◽  
Vol 61 (3) ◽  
pp. 250-253
Author(s):  
Paula Perlea ◽  
◽  
Cristina Coralia Nistor ◽  
Ioana Suciu ◽  
Liana Aminov ◽  
...  

The purpose of the study is to assess the apical sealing ability of the endodontic space using two types of canal filling techniques: vertical compaction of warm gutta-percha and AhPlus sealer compared with Resilon and RealSeal SE. Two sets of extracted human teeth were prepared with rotary instruments, each 20 teeth being obturated by the two techniques: the classical and the adhesive root canal filling. The apical leakage of the root canal filling made with Resilon and RealSeal SE was higher than the one with gutta-percha and AhPlus. The difference was statistically significant.


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