Determination of the Toxicity Cause by Trace Metals on Zebrafish (Danio rerio) Embryo

Water quality deterioration due to inorganic and organic pollutant is a serious issue and the presence of toxic trace metals cause a serious threat to the aquatic ecosystem. Fish embryos have gained interest in risk assessment because of their high sensitivity to pollutants and the ecological relevance. This study investigated the acute toxicity effect of trace metals Arsenic (As), Cadmium (Cd), Mercury (Hg), Lead (Pb), Copper (Cu) and Zinc (Zn) on zebrafish (Danio rerio) embryo. Embryos were exposed to ten different concentrations of individual trace metals and lethality rate was recorded at 24, 48, 72 and 96 hours based on the coagulation of fertilized egg, lack of somite formation, lack of detachment of the tail and lack of heart beat. The results indicated a significant difference between the control and trace metal treated embryo (P< 0.05) and higher mortality rate along the increase of trace metal concentration. Along with the increase of exposure time for Cu, Zn and As, the mortality rate became slower. Sub-lethal and teratogenic deformities such as growth retardation, lack of tail development, lack of eye lens (placode), yolk sac edema, pericardial edema, hemorrhages, shrinkage of chorion and scoliosis were observed in most of the trace metal treated embryos. The results showed the toxic effects to aquatic biota due to trace metals emphasizing the usefulness of zebrafish embryo model for integrated biological hazard assessment.

Download Full-text

Analysis of Trace Metal Contamination in Pahang River and Kelantan River, Malaysia

MATEC Web of Conferences ◽

10.1051/matecconf/201926604003 ◽

2019 ◽

Vol 266 ◽

pp. 04003 ◽

Cited By ~ 1

Author(s):

Norpadzlihatun Manap ◽

Kavitha Sandirasegaran ◽

Noor Shahifah Syahrom ◽

Amnorzahira Amir

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Metal Contamination ◽

Sediment Quality ◽

Primary Objective ◽

Trace Metal Concentration ◽

Trace Metal Contamination ◽

Quality Guidelines ◽

Fish Samples ◽

Negative Impacts

The primary objective of this study is to determine trace metal contamination in environmental samples obtained from Pahang River and Kelantan River, Malaysia which may help to identify the risk of sustainable dredging in these areas. This research also proceeds to compare the trace metal concentration with the National Water Quality Standards of Malaysia, Interim Canadian Sediment Quality Guidelines and Malaysian Food Act 1983 to determine its limits and risks. Samples of water, sediment, snails and fishes were collected and analyzed for As, Cu, Cd, Cr, Fe, Pb, Ni, Mn, and Hg by using atomic absorption spectrophotometer. It was found that the concentration of trace metals namely As, Cu, Cd, Cr, Pb, Ni, and Hg in river water, sediment, snail and fish samples in Pahang River were lower than the maximum allowable limits, except for Fe and Mn. In Kelantan River, the concentration of trace metals indicating that it is contaminated with Fe, Mn, Pb, Cr, Cu, Hg, and As as all trace metals exceeded the maximum allowable limits. Negative impacts may arise, and the river may contaminate more in future if there is no proper management to tackle this issue during execution of dredging activities.

Download Full-text

Characteristics of Atmospheric Metalliferous Particles during Large-Scale Fireworks in Korea

Advances in Meteorology ◽

10.1155/2015/423275 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 3

Author(s):

Zang-Ho Shon ◽

Ju-Hee Jeong ◽

Yoo-Keun Kim

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Large Scale ◽

Background Level ◽

Trace Metal Concentration ◽

Urban Background ◽

Ambient Concentration ◽

Local Background ◽

The Impact ◽

Concentration Levels

The effect of large-scale firework events on urban background trace metal concentrations was investigated using 24 hr data collected over 3 days at three sites in Busan Metropolitan City, Republic of Korea, during the falls (Oct.) of 2011–2013. The firework events increased local background concentrations of trace metals as follows: K (1.72 times), Sr (2.64 times), As (2.86 times), Pb (2.91 times), and Al (5.44 times). The levels of some metals did not always drop to background level one day after the firework event. The contribution of fireworks to trace metal concentration levels (and emissions) for 2011 event was negligible compared to 2012 and 2013 events due to different meteorological conditions (precipitation). In addition, the impact of firework events on the ambient concentration levels of trace metals was likely to be different depending on their chemical speciation. The impact of firework events in Busan on urban air quality (trace metal) was less intense compared to other similar festivals worldwide. The largest emission of trace metals and elements from firework burning was represented by K (128–164 kg), followed by Pb, Cd, Cu, Mg, Ba, As, Al, Ga, Co, and Na.

Download Full-text

Response of diffusive equilibrium in thin films (DET) and diffusive gradients in thin films (DGT) trace metal profiles in sediments to phytodetritus mineralisation

Environmental Chemistry ◽

10.1071/en11075 ◽

2012 ◽

Vol 9 (1) ◽

pp. 41 ◽

Cited By ~ 10

Author(s):

Yue Gao ◽

Martine Leermakers ◽

Annelies Pede ◽

Aurelie Magnier ◽

Koen Sabbe ◽

...

Keyword(s):

Thin Films ◽

Trace Metals ◽

Trace Metal ◽

Phytoplankton Bloom ◽

Marine Ecosystem ◽

Trace Metal Concentration ◽

Water Interface ◽

Overlying Water ◽

Study Laboratory ◽

Diffusive Gradients

Environmental contextContaminated sediments can have a large and lasting effect on marine ecosystems. It was discovered that significant amounts of pollutants, especially arsenic, were released from contaminated sediments during a phytoplankton bloom in the Belgian Continental Zone. Once released to the water column, these pollutants can accumulate up marine food chains and be a source of contaminants to humans. AbstractField data from the Belgian Continental Zone showed elevated trace metal concentrations at the sediment–water interface after the occurrence of a phytoplankton bloom. In the present study, laboratory incubation experiments were used to investigate the effect of the phytodetritus remineralisation process on the release of trace metals from contaminated muddy sediments. This remineralisation process was followed by the measurement of chlorophyll-a and dissolved organic carbon levels in the top sediment layers. Two gel techniques, diffusive equilibrium in thin films (DET) and diffusive gradients in thin films (DGT), were used to assess vertical metal profiles in the sediment pore waters and to calculate the metal effluxes. These metal effluxes compared very well with the trace metal concentration variations in the overlying water of the sediment. Much higher effluxes of Mn, Co and As were observed after 2 days of incubation in the microcosms which received additions of phytodetritus. This trend gradually decreased after 7 days of incubation, suggesting that the elevated efflux of trace metals was proportional to the quantity of phytodetritus mineralised at the sediment–water interface. The release of large amounts of toxic elements from the sediments after phytoplankton blooms can therefore potentially affect the marine ecosystem in the Belgian Continental Zone.

Download Full-text

Serum trace metal association with response to erythropoiesis stimulating agents in incident and prevalent hemodialysis patients

Scientific Reports ◽

10.1038/s41598-020-77311-8 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Michael E. Brier ◽

Jessica R. Gooding ◽

James M. Harrington ◽

Jason P. Burgess ◽

Susan L. McRitchie ◽

...

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Dose Response ◽

Inductively Coupled Plasma ◽

High Sensitivity ◽

Hemodialysis Patients ◽

Chronic Hemodialysis ◽

Drug Dosing ◽

Negatively Associated ◽

Protein Levels

AbstractAlterations in hemodialysis patients’ serum trace metals have been documented. Early studies addressing associations levels of serum trace metals with erythropoietic responses and/or hematocrit generated mixed results. These studies were conducted prior to current approaches for erythropoiesis stimulating agent (ESA) drug dosing guidelines or without consideration of inflammation markers (e.g. hepcidin) important for regulation of iron availability. This study sought to determine if the serum trace metal concentrations of incident or chronic hemodialysis patients associated with the observed ESA response variability and with consideration to ESA dose response, hepcidin, and high sensitivity C-reactive protein levels. Inductively-coupled plasma-mass spectrometry was used to measure 14 serum trace metals in 29 incident and 79 prevalent dialysis patients recruited prospectively. We compared these data to three measures of ESA dose response, sex, and dialysis incidence versus dialysis prevalence. Hemoglobin was negatively associated with ESA dose and cadmium while positively associated with antimony, arsenic and lead. ESA dose was negatively associated with achieved hemoglobin and vanadium while positively associated with arsenic. ESA response was positively associated with arsenic. Vanadium, nickel, cadmium, and tin were increased in prevalent patients. Manganese was increased in incident patients. Vanadium, nickel, and arsenic increased with time on dialysis while manganese decreased. Changes in vanadium and manganese were largest and appeared to have some effect on anemia. Incident and prevalent patients’ chromium and antimony levels exceeded established accepted upper limits of normal.

Download Full-text

Trace metals and the productivity of shelf waters off north west Australia

Marine and Freshwater Research ◽

10.1071/mf9840505 ◽

1984 ◽

Vol 35 (5) ◽

pp. 505 ◽

Cited By ~ 15

Author(s):

D Mackey

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Nutrient Enrichment ◽

High Performance ◽

Copper Ions ◽

Total Concentration ◽

Trace Metal Concentration ◽

North West ◽

Complexing Capacity ◽

Fluorescence Measurements

Oceanographic measurements on the North West Shelf of Australia indicated that nutrient enrichment was derived from a combination of terrestrial run-off and interaction with the sediments. There was no evidence for upwelling. In situ fluorescence measurements suggested that the phytoplankton biomass was low and did not increase in regions of nutrient enrichment. On the continental shelf, the concentrations of copper, nickel and cadmium were typical of unpolluted waters and by using a reverse- phase high performance liquid chromatography absorbent (SEP-PAK), about 20% of the copper was found to be organically bound with smaller amounts of organically bound nickel, zinc and iron and even less organically bound cadmium, chromium and manganese. The copper-complexing capacity of the surface waters (5-10 nM) was comparable to the total concentration of trace metals liable to form organic complexes so that the trace metal speciation may change markedly with small changes in trace metal concentration or complexing capacity. Calculations show that the concentration of free copper ions is too low to have any adverse affects on the biota and it is suggested that the productivity of the water may be affected by the limited bioavailability of essential trace metals.

Download Full-text

Assessment of trace metal in macroalgae and sediment of the Sundarban mangrove estuary

Applied Water Science ◽

10.1007/s13201-019-1134-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Ayesha Siddiqua Asha ◽

A. S. M. Saifullah ◽

Md. Galal Uddin ◽

Md. Shemul Sheikh ◽

Muhammad Jasim Uddin ◽

...

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Significant Positive Correlation ◽

Trace Metal Concentration ◽

Monsoon Period ◽

Period Analysis ◽

Positive Correlation ◽

Mangrove Estuary ◽

Zn And Cu In ◽

Sundarban Mangrove

AbstractThis study was carried out to assess the levels of trace metals (Fe, Zn, Mn and Cu) in the macroalgae and sediment of the Sundarban mangrove estuary in Bangladesh. In this connection, macroalgae, water and sediment samples were collected seasonally (pre-monsoon, monsoon and post-monsoon) for a year from the study area. The surface water of the estuary was found to be quite rich in NO3, PO4 and NH4, which is more than that of the sediment pore water. Trace elements were determined using an atomic absorption spectrophotometer. The amount of trace metals in the samples were found in the order of Fe > Mn > Zn > Cu. Enrichment factor was done for trace metals in sediment and showed non-crustal origin of Zn and Cu in the monsoon period. Analysis of variance revealed the seasonal variation of trace metal concentration in sediment (except Mn; p > 0.05) and macroalgae (except Zn; p > 0.05). Iron in sediment showed significant positive correlation both with Fe (r = 0.84, p < 0.05) and Mn (r = 0.87, p < 0.01) in macroalgae. Similarly, Cu in sediment demonstrated a significant positive correlation with Cu in macroalgae (r = 0.81, p < 0.01). The findings reveal a significant communication of metals in sediment and macroalgae.

Download Full-text

Evaluation of Trace Metal Profile inCymbopogon validusandHyparrhenia hirtaUsed as Traditional Herbs from Environmentally Diverse Region of Komga, South Africa

Journal of Analytical Methods in Chemistry ◽

10.1155/2016/9293165 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Babalwa Tembeni ◽

Opeoluwa O. Oyedeji ◽

Ikechukwu P. Ejidike ◽

Adebola O. Oyedeji

Keyword(s):

South Africa ◽

Trace Metals ◽

Trace Metal ◽

Metal Concentration ◽

Trace Metal Concentration ◽

Eastern Cape ◽

Metal Concentrations ◽

Herbal Products ◽

Plant Parts ◽

Permissible Limits

FAAS was used for the analysis of trace metals in fresh and dry plant parts ofCymbopogon validusandHyparrhenia hirtaspecies with the aim of determining the trace metals concentrations in selected traditional plants consumed in Eastern Cape, South Africa. The trace metal concentration (mg/kg) in the samples of dryCymbopogon validusleaves (DCVL) showed Cu of12.40±1.000; Zn of2.42±0.401; Fe of2.50±0.410; Mn of1.31±0.210; Pb of3.36±0.401 mg/kg, while the samples of freshHyparrhenia hirtaflowers (FHHF) gave Cu of9.77±0.610; Zn of0.70±0.200; Fe of2.11±0.200; Mn of1.15±0.080; Pb of3.15±0.100 mg/kg. Abundance of metal concentrations follows the order: Cu > Fe > Pb > Mn > Zn in the flower samples ofCymbopogon validusandHyparrhenia hirtaspecies. The concentrations of trace metals in both plant parts were below the permissible limits (PL) set by WHO. It is suggested that pharmacovigilance be carried out periodically to improve the quality, safety, and efficiency of various herbal products.

Download Full-text

Sewage sludge application on cultivated soils: effects on runoff and trace metal load

Scientia Agricola ◽

10.1590/s0103-90162009000300012 ◽

2009 ◽

Vol 66 (3) ◽

pp. 368-376 ◽

Cited By ~ 10

Author(s):

Marcelo Valadares Galdos ◽

Isabella Clerici De Maria ◽

Otávio Antonio de Camargo ◽

Sonia Carmela Falci Dechen

Keyword(s):

Trace Metals ◽

Sewage Sludge ◽

Trace Metal ◽

Agricultural Soils ◽

Trace Metal Concentration ◽

High Concentration ◽

Runoff Water ◽

Total Runoff ◽

Water And Sediment ◽

Sludge Application

The use of sewage sludge in agricultural soils as a macro and micronutrient source and as a soil conditioner has been one of the alternatives for its disposal. However, sewage sludge contains trace metals, which are potential sources of pollution. The goal of this study was to evaluate the effect of sewage sludge application on surface water contamination through runoff when it was applied in a soil cultivated with corn. The effect of sludge application on the concentration and load of copper, nickel and zinc and the volume of runoff water and sediment were evaluated. The experiment was set up in plots used to study erosion losses in Campinas, Sao Paulo State, Brazil. The soil is a clayey Rhodic Hapludox. Three treatments were studied: no sewage sludge, sewage sludge to supply the N required by the crop and twice that amount, with four replications. The water and sediment lost by runoff were measured after each rainfall, and sampled for chemical analysis. The volumes of water and sediment lost by runoff decreased after sewage sludge application. The waste application increased trace metal concentration in the runoff water and sediment, especially zinc, which was present in high concentration in the sewage sludge used. Nevertheless, the load of trace metals transported from the plot was mostly dependent on the total runoff volume. Most of the Cu, Zn and Ni losses were via sediment, and occurred in a few highly erosive rainfall events in the period studied.

Download Full-text

Trace metals in sediments from the Yarra River

Marine and Freshwater Research ◽

10.1071/mf9820761 ◽

1982 ◽

Vol 33 (5) ◽

pp. 761 ◽

Cited By ~ 18

Author(s):

M Ellaway ◽

BT Hart ◽

R Beckett

Keyword(s):

Trace Metals ◽

Trace Metal ◽

Metal Concentration ◽

Estuarine Sediments ◽

Trace Metal Concentration ◽

Metal Concentrations ◽

Lead And Zinc ◽

Sediment Fraction ◽

Copper Lead ◽

Yarra River

The distribution and phase association of iron, manganese, cadmium, copper, lead and zinc in bottom sediments taken from the freshwater, estuarine and bay regions of the Yarra River were investigated. The fraction of the estuarine sediments smaller than 20 �m contained substantially higher concentrations of cadmium, copper, lead and zinc than did the corresponding sediment fraction from the upper river and bay regions. Sequential chemical extraction revealed that most of the variability in the trace metal concentration of these sediments was associated with changes in the reducible phase. A substantial increase in trace metal concentrations occurred in going from river to estuarine sediments. For example, the lead concentration increased approximately eightfold (43-375 �g g-1), the zinc fourfold (122-447 �g g-1) and the copper almost twofold (64-106 �g g-1). This was attributed to coagulation and sedimentation of trace-metal- enriched iron and manganese oxides in the estuarine region. The subsequent decrease in metal concentrations in the bay sediments was due to either physical dilution of the contaminated sedlment with material of lower metal concentration or remobilizatlon of the trace metals from sediments deposited in the bay.

Download Full-text

Seasonal and Spatial Variation in Dissolved Heavy Metals in Liaodong Bay, China

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph19010608 ◽

2022 ◽

Vol 19 (1) ◽

pp. 608

Author(s):

Weijun Guo ◽

Jibing Zou ◽

Sihong Liu ◽

Xuewen Chen ◽

Xiangpeng Kong ◽

...

Keyword(s):

Heavy Metals ◽

Trace Metals ◽

Trace Metal ◽

Spatial Gradient ◽

Trace Metal Concentration ◽

Surface Seawater ◽

Liaodong Bay ◽

Pollution Levels ◽

Four Seasons ◽

Dissolved Heavy Metals

Spatial–seasonal variations in dissolved heavy metals in surface seawater were analyzed based on surveys at 87 sampling sites and water samples from six rivers across Liaodong Bay. The concentrations of copper (Cu), lead (Pb), cadmium (Cd), and zinc (Zn) had ranges of 0.20–40.00 (5.45 ± 5.67), 0.51–33.64 (4.68 ± 3.93), 0.03–13.47 (2.22 ± 2.01), and 0.50–80.09 μg/L (14.22 ± 16.32), respectively, throughout the four seasons of 2020. The trace metal concentration showed a spatial gradient of high to low from river to estuary and from inshore to offshore areas. A combination of pollution levels and marine sensitivity was employed to assess the pollution degree of the heavy metals. As a whole, the single pollution factors of trace metals in Liaodong Bay were ranged in the order Pb > Zn > Cu > Cd. The total pollution degree was relatively high in autumn and summer due to increased riverine inputs after the rainy season, while relatively low in spring and winter. These findings provide baseline data for future targeting policies to protect marine environments in Liaodong Bay.

Download Full-text