Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas

This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.

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HISTOPATHOLOGICAL OF DIABETES MELLITUS WHITE RAT TESTICLE AFTER GIVEN MORINGA LEAF EXTRACT

Buletin Veteriner Udayana ◽

10.24843/bulvet.2018.v10.i02.p11 ◽

2018 ◽

pp. 176

Author(s):

Raodatul Jannah ◽

Ni Luh Eka Setiasih ◽

Putu Suastika

Keyword(s):

Diabetes Mellitus ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Moringa Oleifera ◽

White Rats ◽

Moringa Oleifera Leaves ◽

Rat Strain ◽

Testis Structure ◽

Moringa Leaf Extract

This study aimed to determine changes in testicular histopathological of the rat strain wistar diabetes mellitus experienced. This study used 24 white rats (Rattus norvegicus) strain Wistar which were divided into six groups: administration of Moringa oleifera leaves P1 (extract dose 0 mg/kg bw) , P2 (100 mg/kg bw) , P3 (200 mg/kg bw), P4 (300 mg/kg bw) , P5 (400 mg/kg bw) and P6 ( 500 mg/kg bw) . In the end of administration, the diabetic Wistar rats were sacrificed and their testes were taken to make the histopathological preparations using haematoxylin and eosin staining. The observed variables are fat degeneration and necrosis. The results show that moringa leaves can improve histopathology testis structure of diabetes mellitus white rats. Extract of Moringa leaves (Moringa oleifera) dose of 400 mg/kg body weight has the best effect repair damage to the histopathological testis.

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Ekstrak Daun Kelor (Moringa Oleifera Lamk) dalam Menurunkan Kadar Glukosa Darah pada Tikus (Rattus novergicus)

bionature ◽

10.35580/bionature.v21i1.13921 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Mursito, S. Mursito, S. ◽

Bialangi Lilies ◽

Hayyatun Mawaddah

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Moringa Oleifera ◽

Biology Education ◽

Study Program ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Independent Variable ◽

Moringa Leaf Extract

Abstract. The purposes of this study were: 1) obtain information on the benefits of Moringa leaf extract which has the effectiveness in reducing blood glucose levels. specifically on Rattus norvegicus, 2) obtaining information about the concentration of Moringa oleifera leaf extract) has effectiveness in reducing blood glucose levels, specifically on Rattus norvegicus. Research conducted in the Laboratory of Biology Education Study Program at the Tadulako University FKIP in May until August 2018 and is an experimental study using the RAL method. The independent variable In this study was Moringa oleifera leaf extract, and the dependent variable was the glucose level on Rattus Norvegicus. Data analysis in this research was carried out using, analysis of quantitative Anava data with the help of SPSS Version18. Significant results of an analysis of variance (ANAVA) obtained a significance value of 0,000<α (0.05), which means rejecting Ho and accepting H1. Thus it can be explained that Moringa leaf extract has a significant effect on the reduction in blood glucose levels in rats (Rattus governors). When seen in the results of the analysis of the advanced test using the Duncan test, the best concentration in reducing glucose levels is on the M4 treatment, with Duncan test results that are different from the other, amounting to 113.40. Keywords: moringa oleifera lamk, blood glucose, rattus novergicus.

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UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.30015 ◽

2020 ◽

Vol 9 (3) ◽

pp. 325

Author(s):

Alyah A.P Tamimi ◽

Edwin De Queljoe ◽

Jainer P. Siampa

Keyword(s):

Rattus Norvegicus ◽

Analgesic Effect ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Negative Control ◽

Test Material ◽

Control Group ◽

White Rats ◽

Wistar Strain ◽

Moringa Oleifera Lam

ABSTRACTAnalgesic medicinals are medicinals that can inhibit pain. Moringa leaves (Moringa oleifera Lam.) are known to contains alkaloid and flavonoid which have analgesic effects.The purpose of this study was to determine the analgesic effects of the ethanol extract of Moringa leaves on male white rats wistar strain (Rattus norvegicus). A total of 15 rats were divided into 5 treatment groups, namely the negative control group (CMC), positive control group (paracetamol), and the group of ethanol extract of Moringa leaves with different doses of 0.1 g, 0.2 g, and 0.4 g. Rat response was observed before giving test material and after giving test material from the 30th, 60th, 90th, and 120th minutes. Data were analyzed using ANOVA then continued with LSD test. The results showed that the ethanol extract of Moringa leaves with dosages of 0.1 g, 0.2 g, and 0.4 g had an analgesic effect on male white rats wistar strain. And among these three doses the best in providing analgesic effect is a dose of 0.4 g. Keywords: Analgesic, Moringa leaf (Moringa oleifera Lam.), Rattus norvegicus. ABSTRAKObat analgesik merupakan obat yang dapat menghambat rasa nyeri. Daun kelor (Moringa oleifera Lam.) diketahui mengandung alkaloid dan flavonoid yang memiliki efek analgesik. Tujuan penelitian ini untuk mengetahui adanya efek analgesik dari ekstrak etanol daun kelor (Moringa oleifera Lam.) pada tikus putih jantan galur wistar (Rattus norvegicus). Sebanyak 15 ekor tikus dibagi 5 kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok kontrol positif, dan kelompok ekstrak etanol daun kelor dengan dosis berbeda 0,1 g, 0,2 g, dan 0,4 g. Respon tikus diamati sebelum pemberian bahan uji dan setelah pemberian bahan uji dari menit ke 30, 60, 90, dan 120. Data dianalisis menggunakan ANOVA kemudian dilanjutkan uji LSD. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kelor dengan dosis 0,1 g 0,2 g, dan 0,4 g memiliki efek analgesik terhadap tikus putih jantan galur wistar. Dan diantara ketiga pemberian dosis tersebut yang paling baik dalam memberikan efek analgesik yaitu dosis 0,4 g. Kata kunci: Analgesik, Daun Kelor (Moringa oleifera Lam.), Rattus norvegicus.

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Gambaran histopatologik hati tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.13331 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Satriani Syahrin ◽

Carla Kairupan ◽

Lily Loho

Keyword(s):

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Moringa Oleifera ◽

Inflammatory Cells ◽

Negative Control ◽

Group A ◽

Group B ◽

Group D ◽

Moringa Leaf Extract

Abstract: Moringa (Moringa oleifera) is a traditional plant, which is known to treat liver disease. The effect of moringa is related to the antioxidant activity of its compounds such as quercetin and flavonoid silymarin which are useful as liver protector. Carbon tetraclorida is a hepatotoxic substance that is usually used as an inducer of liver damage in studies related to hepatoprotector activity of a substance. This study aimed to describe liver histopathological features of Wistar rats fed with moringa leaf extract after CCl4 induction. This was an experimental study using 24 Wistar rats (Rattus norvegicus) which were divided into 4 groups. Group A, the negative control, was terminated at day 6th, and the other groups (B, C, and D) were induced with CCl4 0.05 cc/day for 5 days. After CCl4 induction, group B were terminated at day 6th; group C was treated with moringa leaf extract 100 mg/day for 5 days and was terminated at day 11th; group D received no treatment for 5 days and was terminated at day 11th. The results showed that groups induced with CCl4 for 5 days showed the presence of inflammatory cells and fatty cells. The groups treated with moringa leaf extract 100mg/day for 5 days after CCl4 induction 0.05 cc/day exhibited regeneration of liver cells in nearly all lobules. Conclusion: Administration of moringa leaf extract of 100mg/day could accelerate liver cell regeneration of Wistar rats after induction of CCl4 0.05cc/day.Keywords: moringa leaf extract, carbon tetrachloride, histopathologogical image of liverAbstrak: Kelor merupakan tanaman tradisional yang diketahui dapat mengobati penyakit hati. Khasiat obat tanaman kelor dihubungkan dengan kandungan senyawa kimia quercetin dan silymarin golongan flavonoid dengan aktivitas antioksidan yang dapat melindungi dan mengobati kerusakan hati. Karbon tetraklorida merupakan zat hepatotoksik yang lazim dipakai sebagai penginduksi kerusakan hati dalam pengujian aktivitas hepatoprotektor suatu zat. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik hati tikus wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi CCl4. Jenis penelitian ini ialah eksperimental menggunakan 24 ekor tikus Wistar spesies Rattus norvegicus yang dibagi dalam 4 kelompok. Kelompok A merupakan kelompok kontrol negatif, diterminasi pada hari ke-6; kelompok B,C, dan D (kelompok perlakuan) diberi CCl4 dengan dosis 0,05 cc/hari selama 5 hari. Setelah pemberian CCl4, kelompok B langsung diterminasi pada hari ke-6; kelompok C diberi ekstrak daun kelor 100 mg/hari selama 5 hari, diterminasi pada hari ke-11; kelompok D tidak diberi perlakuan selama 5 hari, diterminasi pada hari ke-11. Hasil penelitian menunjukkan bahwa kelompok tikus yang diinduksi CCl4 selama 5 hari secara histopatologik memperlihatkan sel-sel radang dan perlemakan sel. Kelompok tikus yang diberi ekstrak daun kelor 100 mg/hari selama 5 hari setelah diinduksi CCl4 0,05 cc/hari menunjukkan regenerasi sel hati, hampir di seluruh lobuli hati. Simpulan: Pemberian ekstrak daun kelor 100 mg/hari setelah induksi CCl4 0,05 cc/hari dapat mempercepat regenerasi sel hati tikus Wistar yang mengalami cedera akibat CCl4.Kata kunci: ekstrak daun kelor, karbon tetraklorida, gambaran histopatologik hati

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Using design expert d-optimal for formula optimization of functional drink that enriched with moringa leaf extract (Moringa oleifera)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/759/1/012002 ◽

2021 ◽

Vol 759 (1) ◽

pp. 012002

Author(s):

M A Kahfi ◽

A N Sutisna ◽

H Ainia ◽

A R Cecep

Keyword(s):

Leaf Extract ◽

Moringa Oleifera ◽

Design Expert ◽

Functional Drink ◽

Moringa Leaf Extract

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Analysis Of Protein Levels With Giving Kelor Leaf Extract (Moringa Oleifera) On Soy Milk

JOURNAL FOR QUALITY IN PUBLIC HEALTH ◽

10.30994/jqph.v3i2.110 ◽

2020 ◽

Vol 3 (2) ◽

pp. 604-610

Author(s):

Fajar Nur Atmaja

Keyword(s):

Protein Content ◽

Quantitative Research ◽

Leaf Extract ◽

Moringa Oleifera ◽

Agricultural Product ◽

Soy Milk ◽

Safety Testing ◽

Protein Levels ◽

Food Quality And Safety ◽

Moringa Leaf Extract

Soy milk is a beverage product that is currently being favored by the people of Indonesia, to increase the protein content in soy milk needs to be substituted for other ingredients using Moringa Oleifera leaf extract. The purpose of this study was to analyze the organoleptic acceptability of soy milk by giving Moringa Oleifera leaf extract. This research is quantitative research with experimental research design using the True Experiment research method. This research was conducted in December 2018 until February 2019 at the Food Quality and Safety Testing Laboratory of the Agricultural Product Technology Department of the Faculty of Agriculture, University of Brawijaya Malang to test the protein content while the Organoleptic Test was carried out at STIKeS Satria Bhakti Nganjuk. The sample size is 20 panelists. The independent variable in this study was Moringa leaf extract while the protein content of soy milk. Data was collected using an organoleptic sensory test assessment table and a protein content test sheet using the Kjeldahl method. Data were analyzed using ANNOVA test with the results of H0 rejected and H1 accepted with a significant value <0.05 The results of ANOVA analysis get F count value of 105,405 and F table for testing 20 samples to get F table 4.35. So that it can be concluded that the addition of Moringa leaf extract 0% to 10% Significant and simultaneous organoleptic. Addition of Moringa Oleifera leaf extract to soy milk affected organoleptic acceptability and increased protein content of soy milk in accordance with the percentage of Moringa leaf extract levels added.

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