scholarly journals HISTOPATHOLOGICAL OF DIABETES MELLITUS WHITE RAT TESTICLE AFTER GIVEN MORINGA LEAF EXTRACT

2018 ◽  
pp. 176
Author(s):  
Raodatul Jannah ◽  
Ni Luh Eka Setiasih ◽  
Putu Suastika
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
White Rats ◽  
Moringa Oleifera Leaves ◽  
Rat Strain ◽  
Testis Structure ◽  
Moringa Leaf Extract

This study aimed to determine changes in testicular histopathological of the rat strain wistar diabetes mellitus experienced. This study used 24 white rats (Rattus norvegicus) strain Wistar which were divided into six groups: administration of Moringa oleifera leaves P1 (extract dose 0 mg/kg bw) , P2 (100 mg/kg bw) , P3 (200 mg/kg bw), P4 (300 mg/kg bw) , P5 (400 mg/kg bw)  and P6 ( 500 mg/kg bw) . In the end of administration, the diabetic Wistar rats were sacrificed and their testes were taken to make the histopathological preparations using haematoxylin and eosin staining. The observed variables are fat degeneration and necrosis. The results show that moringa leaves can improve histopathology testis structure of diabetes mellitus white rats. Extract of Moringa leaves (Moringa oleifera) dose of 400 mg/kg body weight has the best effect repair damage to the histopathological testis.

scholarly journals Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas

2021 ◽  
Vol 10 (3) ◽  
pp. 65
Author(s):  
Arinda Octavia ◽  
Budi Utomo ◽  
Sri Pantja Madyawati ◽  
Tri Wahyu Suprayogi ◽  
Agus Sunarso ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
White Rats ◽  
Moringa Leaf Extract ◽  
Moringa Oleifera Lam

 This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.

scholarly journals Gambaran histopatologik hati tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Satriani Syahrin ◽  
Carla Kairupan ◽  
Lily Loho
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Moringa Leaf Extract

Abstract: Moringa (Moringa oleifera) is a traditional plant, which is known to treat liver disease. The effect of moringa is related to the antioxidant activity of its compounds such as quercetin and flavonoid silymarin which are useful as liver protector. Carbon tetraclorida is a hepatotoxic substance that is usually used as an inducer of liver damage in studies related to hepatoprotector activity of a substance. This study aimed to describe liver histopathological features of Wistar rats fed with moringa leaf extract after CCl4 induction. This was an experimental study using 24 Wistar rats (Rattus norvegicus) which were divided into 4 groups. Group A, the negative control, was terminated at day 6th, and the other groups (B, C, and D) were induced with CCl4 0.05 cc/day for 5 days. After CCl4 induction, group B were terminated at day 6th; group C was treated with moringa leaf extract 100 mg/day for 5 days and was terminated at day 11th; group D received no treatment for 5 days and was terminated at day 11th. The results showed that groups induced with CCl4 for 5 days showed the presence of inflammatory cells and fatty cells. The groups treated with moringa leaf extract 100mg/day for 5 days after CCl4 induction 0.05 cc/day exhibited regeneration of liver cells in nearly all lobules. Conclusion: Administration of moringa leaf extract of 100mg/day could accelerate liver cell regeneration of Wistar rats after induction of CCl4 0.05cc/day.Keywords: moringa leaf extract, carbon tetrachloride, histopathologogical image of liverAbstrak: Kelor merupakan tanaman tradisional yang diketahui dapat mengobati penyakit hati. Khasiat obat tanaman kelor dihubungkan dengan kandungan senyawa kimia quercetin dan silymarin golongan flavonoid dengan aktivitas antioksidan yang dapat melindungi dan mengobati kerusakan hati. Karbon tetraklorida merupakan zat hepatotoksik yang lazim dipakai sebagai penginduksi kerusakan hati dalam pengujian aktivitas hepatoprotektor suatu zat. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik hati tikus wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi CCl4. Jenis penelitian ini ialah eksperimental menggunakan 24 ekor tikus Wistar spesies Rattus norvegicus yang dibagi dalam 4 kelompok. Kelompok A merupakan kelompok kontrol negatif, diterminasi pada hari ke-6; kelompok B,C, dan D (kelompok perlakuan) diberi CCl4 dengan dosis 0,05 cc/hari selama 5 hari. Setelah pemberian CCl4, kelompok B langsung diterminasi pada hari ke-6; kelompok C diberi ekstrak daun kelor 100 mg/hari selama 5 hari, diterminasi pada hari ke-11; kelompok D tidak diberi perlakuan selama 5 hari, diterminasi pada hari ke-11. Hasil penelitian menunjukkan bahwa kelompok tikus yang diinduksi CCl4 selama 5 hari secara histopatologik memperlihatkan sel-sel radang dan perlemakan sel. Kelompok tikus yang diberi ekstrak daun kelor 100 mg/hari selama 5 hari setelah diinduksi CCl4 0,05 cc/hari menunjukkan regenerasi sel hati, hampir di seluruh lobuli hati. Simpulan: Pemberian ekstrak daun kelor 100 mg/hari setelah induksi CCl4 0,05 cc/hari dapat mempercepat regenerasi sel hati tikus Wistar yang mengalami cedera akibat CCl4.Kata kunci: ekstrak daun kelor, karbon tetraklorida, gambaran histopatologik hati

scholarly journals Oral L-Arginine lower neovascularization and the number of fibroblasts but failed to increase nitric oxide and epithelialization in the healing process of male white diabetic wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 24
Author(s):  
Fenni Liem ◽  
Wimpie Pangkahila ◽  
I Wayan Weta
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Healing Process ◽  
Intervention Group ◽  
Control Group ◽  
White Rats ◽  
Nitric Oxide Level ◽  
The Mean

Introduction: Oral L-Arginine is a conditional essential amino acid that plays a role in wound healing in DM. The role of arginine in diabetic wounds is by enhancing blood circulation in the injured area and increasing oxygen supply to the wound tissue. The purpose of this study to prove the administration of oral L-Arginine toward vascularization status in wound healing of male white rats wistar diabetes mellitus. Methods:   A randomized posttest only control group study using with 36 diabetic induced wistar rats (Rattus Norvegicus) aged 2-3 months and weighing 180-200gram which then divided randomly into two groups. Nitric oxide level was measured on the third day and each group was then further divided into two groups for examination of neovascularization, fibroblasts and epithelialization on the seventh day and on the tenth day. Results: Administration oral L-Arginine failed to induce any significant change in Nitric Oxide level and wound gap closure. On the other hand, the results showed that the mean neovascularization was significantly different between the two groups on the 10th day (Control group vs intervention group: 4.22±1922 vs1.89±1364; p=0.009). In addition, the mean number of fibroblast at the 10th day was also significantly different (Control group vs intervention group: 74.11±28.57 vs 38.11±20.90; p=0.008). Conclusion: In conclusion, oral L-Arginine did not significantly affect nitric oxide and epithelialization while decreased neovascularization and the number of fibroblasts on day tenth in the healing process of male white rats diabetes mellitus

scholarly journals EFEKTIFITAS EKSTRAK DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP GAMBARAN HISTOPATOLOGI PANKREAS PADA TIKUS YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 11 (1) ◽  
pp. 15-24
Author(s):  
Ruswandono Wirjatmadja ◽  
Rondius Solfaine ◽  
Dian Ayu Kasrtikasari ◽  
Anisa Novia Wati
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Inflammatory Cells ◽  
Tithonia Diversifolia ◽  
The Moon ◽  
Male Wistar Rats ◽  
Made In

This study was conducted to determine the histopathological picture of the pancreas by induction of alloxan and extracts of the moon flower (tithonia diversifolia) in wistar rats (rattus norvegicus). A total of 24 male Wistar rats with 4 treatments were control P0, alloxan P1 with 1% CMC Na, P2 alloxan with 100mg / kgBB extract, alloxan P3 with 200mg / kgBB extract. Mice after said diabetes mellitus with a number> 135 mg / dL were then given treatment with 1% CMC Na on P1 and lunar leaf extract at P2 and P3, necropsy was carried out on the 15th day and the pancreatic organs were taken to be made in the form of preparations with coloring. HE, lesions observed Degeneration, Inflammatory Cells and Necrosis using a microscope, the results were analyzed using Kruskal wallis followed by Mann withney. The data obtained by degeneration in the kruskal wallis test were very significantly different because there were significant differences (P = 0.01), inflammation cells in the Kruskal wallis test were very significantly different because there were significant differences (P = 0.00), and for necrosis in the Kruskal wallis test significantly different because there are significant differences (0.02).

scholarly journals Ekstrak Daun Kelor (Moringa Oleifera Lamk) dalam Menurunkan Kadar Glukosa Darah pada Tikus (Rattus novergicus)

bionature ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Mursito, S. Mursito, S. ◽  
Bialangi Lilies ◽  
Hayyatun Mawaddah
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Biology Education ◽  
Study Program ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Independent Variable ◽  
Moringa Leaf Extract

Abstract. The purposes of this study were: 1) obtain information on the benefits of Moringa leaf extract which has the effectiveness in reducing blood glucose levels. specifically on Rattus norvegicus, 2) obtaining information about the concentration of Moringa oleifera leaf extract) has effectiveness in reducing blood glucose levels, specifically on Rattus norvegicus. Research conducted in the Laboratory of Biology Education Study Program at the Tadulako University FKIP in May until August 2018 and is an experimental study using the RAL method. The independent variable In this study was Moringa oleifera leaf extract, and the dependent variable was the glucose level on Rattus Norvegicus. Data analysis in this research was carried out using, analysis of quantitative Anava data with the help of SPSS Version18. Significant results of an analysis of variance (ANAVA) obtained a significance value of 0,000<α (0.05), which means rejecting Ho and accepting H1. Thus it can be explained that Moringa leaf extract has a significant effect on the reduction in blood glucose levels in rats (Rattus governors). When seen in the results of the analysis of the advanced test using the Duncan test, the best concentration in reducing glucose levels is on the M4 treatment, with Duncan test results that are different from the other, amounting to 113.40. Keywords: moringa oleifera lamk, blood glucose, rattus novergicus.

scholarly journals The potential effect of Moringa oleifera leaves extract on vascular endothelial growth factor expression in Wistar rat oral cancer cells

2019 ◽  
Vol 52 (2) ◽  
pp. 71
Author(s):  
Dwicha Rahma Nuriska Hartono ◽  
Theresia Indah Budhy Sulisetyawati ◽  
Edhi Jularso
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Control Group ◽  
Vegf Expression ◽  
Treatment Groups ◽  
Moringa Oleifera Leaves ◽  
Oral Cancer Cells

Background: Cancer is a disease characterized by abnormal and uncontrolled growth of tissue cells. In Indonesia, cancer ranks as the fifth largest cause of mortality, while it is the second largest worldwide. Cancer affected by angiogenesis, the process of forming new blood vessels to provide the nutrient and oxygen necessary for tumor growth. Vascular endothelial growth factor (VEGF) represents a pro-angiogenic factor. Moringa oleifera leaf extract can be used as an anticancer agent by reducing the expression of VEGF. Purpose: The study aimed to prove that Moringa oleifera leaf extract can reduce VEGF expression in benzopyrene-induced oral cancer cells of Wistar rats. Methods: This was an experimental laboratory research with posttest-only control group design. All experimental subjects presented symptoms of cancer following induction with 8 mg/KgBW of benzopyrene. The sample consisted of 28 Wistar rats, divided into four groups, namely; a control group (K) whose members were administered with only aquadest and three treatment groups (P) treated with Moringa oleifera leaves extract at percentages of 3.125% (P1), 4.6875% (P2) and 6.25% (P3) respectively. Observation of VEGF expression was undertaken by means of immunohistochemical staining. Results: A decrease in VEGF expression occurred in all treatment groups when compared with the control group. A significant difference existed between the control group (K) and the treatment group (P1), while there were no significant differences between the treatment groups (P1, P2 and P3). Conclusion: Moringa oleifera leaf extract with a percentage of 3.125% proved most effective at reducing VEGF expression in oral cancer cells in Wistar rats.

scholarly journals Analysis Of Protein Levels With Giving Kelor Leaf Extract (Moringa Oleifera) On Soy Milk

2020 ◽  
Vol 3 (2) ◽  
pp. 604-610
Author(s):  
Fajar Nur Atmaja
Keyword(s):  
Protein Content ◽  
Quantitative Research ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Agricultural Product ◽  
Soy Milk ◽  
Safety Testing ◽  
Protein Levels ◽  
Food Quality And Safety ◽  
Moringa Leaf Extract

Soy milk is a beverage product that is currently being favored by the people of Indonesia, to increase the protein content in soy milk needs to be substituted for other ingredients using Moringa Oleifera leaf extract. The purpose of this study was to analyze the organoleptic acceptability of soy milk by giving Moringa Oleifera leaf extract. This research is quantitative research with experimental research design using the True Experiment research method. This research was conducted in December 2018 until February 2019 at the Food Quality and Safety Testing Laboratory of the Agricultural Product Technology Department of the Faculty of Agriculture, University of Brawijaya Malang to test the protein content while the Organoleptic Test was carried out at STIKeS Satria Bhakti Nganjuk. The sample size is 20 panelists. The independent variable in this study was Moringa leaf extract while the protein content of soy milk. Data was collected using an organoleptic sensory test assessment table and a protein content test sheet using the Kjeldahl method. Data were analyzed using ANNOVA test with the results of H0 rejected and H1 accepted with a significant value <0.05 The results of ANOVA analysis get F count value of 105,405 and F table for testing 20 samples to get F table 4.35. So that it can be concluded that the addition of Moringa leaf extract 0% to 10% Significant and simultaneous organoleptic. Addition of Moringa Oleifera leaf extract to soy milk affected organoleptic acceptability and increased protein content of soy milk in accordance with the percentage of Moringa leaf extract levels added.

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