THE INHIBITORY ACTIVITY OF KELAKAI LEAF EXTRACT AGAINST THE GROWTH OF Porphyromonas gingivalis ATCC® 33277™

ABSTRACTBackground: Chronic periodontitis is an infectious disease that causes damage on periodontal ligament and alveolar bone. The severity of periodontitis is caused by several types of bacterial species which one of them is Porphyromonas gingivalis bacteria with a prevalence of 85% in oral cavity. The extract of kelakai leaf contained antibacterial in the form of flavonoid, alkaloid, tannin, and steroid. Flavonoid consists of some chemical compounds which is one of them is quercetin. The level of quercetin in kelakai leaf is 503.56 mgQE/g. From some secondary metabolites, kelakai leaf has inhibitory power toward gram negative bacterial, Porphyromonas gingivalis. Objective: This research was intended to know the activity of inhibitory power of kelakai leaf toward Porphyromonas gingivalis bacteria. Method: This research was an experimental research consisted of 5 experimental groups that were group of kelakai leaf extract on the concentrations of 100 mh/ml, 75 mg/ml, 50mg/ml, and 25 mg/ml and the control group (0.2% chlorhexidine). Each treatment was done in 4 repetitions. The test of inhibitory power used diffusion method by measuring the inhibitory zone around the growth of Porphyromonas gingivalis on Mueller Hinton Agar media. The data were analyzed by using One Way Anova 95% and then continued with LSD. Results: Based on the LSD test, it was known that the extract of Kelakai leaf had inhibitor power activity toward Porphyromonas gingivalis. The highest inhibitory zone was on the concentration of 100 mg/ml with inhibitory zone of 14.61 mm. Conclusion: The extract of kelakai leaf had inhibitory power activity toward Porphyromonas gingivalis bacteria in vitro. Keywords: 0.2% chlorhexidine, Diffusion method, Inhibitory power, Stenochlaena palustris extract, Porphyromonas gingivalis.

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THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.28435 ◽

2018 ◽

Vol 11 (12) ◽

pp. 411

Author(s):

Ameta Primasari ◽

Minasari Nasution ◽

Nurul Hidayati Arbi ◽

Dini Permata Sari ◽

Mohammad Basyuni

Keyword(s):

Leaf Extract ◽

Brain Heart Infusion ◽

Aggregatibacter Actinomycetemcomitans ◽

Negative Control ◽

Plate Count ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

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Microwave-Assisted Synthesis, Structural Characterization and Assessment of the Antibacterial Activity of Some New Aminopyridine, Pyrrolidine, Piperidine and Morpholine Acetamides

Molecules ◽

10.3390/molecules26030533 ◽

2021 ◽

Vol 26 (3) ◽

pp. 533

Author(s):

Abdulmajeed S. H. Alsamarrai ◽

Saba S. Abdulghani

Keyword(s):

Antibacterial Activity ◽

Bacterial Species ◽

Antibacterial Properties ◽

Molecular Structures ◽

Secondary Amines ◽

Diffusion Method ◽

Microwave Assisted Synthesis ◽

Mueller Hinton ◽

Mueller Hinton Agar

A series of new acetamide derivatives 22–28 of primary and secondary amines and para-toluene sulphinate sodium salt have been synthesized under microwave irradiation and assessed in vitro for their antibacterial activity against one Gram-positive and two Gram-negative bacterial species such as S. pyogenes, E. coli, and P. mirabilis using the Mueller-Hinton Agar diffusion (well diffusion) method. The synthesized compounds with significant differences in inhibition diameters and MICs were compared with those of amoxicillin, ampicillin, cephalothin, azithromycin and doxycycline. All of the evaluated acetamide derivatives were used with varying inhibition concentrations of 6.25, 12.5, 37.5, 62.5, 87.5, 112.5 and 125 µg/mL. The results show that the most important antibacterial properties were displayed by the synthetic compounds 22 and 24, both of bear a para-chlorophenyl moiety incorporated into the 2-position moiety of acetamide 1. The molecular structures of the new compounds were determined using the FT-IR and 1H-NMR techniques.

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Microwave-assisted Synthesis, Structural Characterization of Amino Pyridines, Pyrrolidine, Piperidine, Morpholine, Acetamides, and Assessment of Their Antibacterial Activity

10.20944/preprints202010.0077.v1 ◽

2020 ◽

Author(s):

Abdulmajeed Alsamarrai ◽

Saba Abdulghani

Keyword(s):

Antibacterial Activity ◽

Bacterial Species ◽

Antibacterial Properties ◽

Molecular Structures ◽

Diffusion Method ◽

Microwave Assisted Synthesis ◽

Mueller Hinton ◽

Mueller Hinton Agar ◽

New Compounds

A sequence of new acetamide derivatives 9-15 of primary, secondary amine, and para-toluene sulphinate sodium salt have been synthesized under microwave irradiation and assessed in vitro for their antibacterial activity against one Gram-positive and two Gram-negative bacterial species such as S. pyogenes, E. coli, and P.mirabilis using the Mueller-Hinton Agar diffusion (well diffusion) method. The synthesized compounds with significant differences in inhibition diameters and MICs were compared with those of amoxicillin, ampicillin, cephalothin, azithromycin and doxycycline. All of the evaluated acetamide derivatives were used with varying inhibition concentrations of 6.25, 12.5, 37.5, 62.5, 87.5, 112.5 and 125 µg/ml. The results show that the most important antibacterial properties exercised by the synthetic compounds 9 and 11 bearing para-chlorophenyl moiety incorporated into the 2-position moiety of acetamide 2. The molecular structures of the new compounds were determined using FT-IR, 1H-NMR techniques.

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Uji Daya Hambat Ekstrak Daun Salam (Syzygium polyanthum wight) Terhadap Pertumbuhan Bakteri Porphyromonas Gingivalis Secara In Vitro

Andalas Dental Journal ◽

10.25077/adj.v9i1.98 ◽

2021 ◽

Vol 9 (1) ◽

pp. 19-28

Author(s):

Monalisa Monalisa ◽

Erly Erly ◽

Aria Fransiska

Keyword(s):

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Leaf Extract ◽

Bacterial Colonization ◽

Inhibition Zone ◽

Control Group ◽

Significant Difference ◽

One Way Anova ◽

Paper Disc

Chronic periodontitis is the most common type of periodontitis. The main cause of chronic periodontitis is bacterial colonization of plaque. Porphyromonas gingivalis is the main pathogen and has the highest prevalence of chronic periodontitis. Bay leaf extract (Syzygium polyanthum wight) is believed to have the pharmacological effect that can be used as antibacterial. The purpose of this research was to determine the inhibitory of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The method of this research was experimental laboratories with posttest only control group design. There were 30 samples divided into 6 groups, namely bay leaf extract concentration of 2.5%, 5%, 10%, 20%, 40% and Dimethyl Sulfoxide (DMSO) as a control. Inhibitory test using the Kirby-Bauer method with a paper disc on Mueller Hinton medium agar. Inhibition zone that created around paper disc was measured with the sliding caliper. The results were analyzed with One Way Anova and Post Hoc LSD test. The results of this research showed that the mean of bay leaf extract 40% had the largest inhibition zone that was 7,6 mm and the lowest formed by bay leaf extract 2,5% that was 1,94 mm. One Way Anova test showed that there was a significant difference among the group (p<0,05). The conclusion of this research there was significant difference inhibition among 2,5%, 5%, 10%, 20%, and 40% concentration of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The higher concentration of bay leaf extract resulting the greater inhibition zone.

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OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO

Interdental Jurnal Kedokteran Gigi (IJKG) ◽

10.46862/interdental.v17i2.2870 ◽

2021 ◽

Vol 17 (2) ◽

pp. 97-102

Author(s):

Dwis Syahrul ◽

◽

Dwis Syahriel ◽

Keyword(s):

Streptococcus Mutans ◽

Leaf Extract ◽

Culture Medium ◽

Ocimum Basilicum ◽

Optimal Concentration ◽

Control Group ◽

Treatment Groups ◽

Significant Difference ◽

Inhibitory Power

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

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Effect of blue laser on viability of Proteus mirabilis

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.5.6 ◽

2021 ◽

pp. 1438-1446

Author(s):

Mawada M. Funjan

Keyword(s):

Exposure Time ◽

Proteus Mirabilis ◽

Bacterial Species ◽

Test System ◽

Blue Laser ◽

Diffusion Method ◽

Biochemical Tests ◽

Burn Wounds ◽

Antibiotic Susceptibility Test

The usage of blue laser has been considered as a therapeutic approach to prohibit the viability of bacterial species, but there is no agreement about optimum parameters to be used. The aim of this project is to study the influence of blue laser (450 nm) on the viability of the gram-negative bacteria Proteus mirabilis isolated from burn wounds, using different exposure times (i.e. doses) in vitro. Seventy swab samples were collected from burn wounds of patients admitted to the burns unit in AL-Yarmouk teaching hospital in Baghdad, during the period from June to August 2019. The Bacteria were isolated and identified depending on their culture characteristics, biochemical tests, gram staining, and morphology, being finally confirmed by API 20E Test System. By using the disk diffusion method, susceptibility of the isolates to 12 different antibiotics was examined. One isolate of P. mirabilis was elected according to susceptibility to all antibiotics used. To prepare bacterial solution, P. mirabilis was mixed with normal saline solution. Dilution of 10-6 cell/ml for p. mirabilis was selected from other serial dilutions. A number of colonies and colony forming units (CFUs/ml) were achieved and correlated to controls. P. mirabilis was irradiated by blue diode laser (450 nm, 500mw) and exposed to different doses (24, 48, 72, 96, 120J/cm2) corresponding to respective exposure times (4, 8,12,16,20 minutes). The results of antibiotic susceptibility test indicate that the entire isolates of P. mirabilis were multidrug resistant. With the increase in laser dose (exposure times), the number of colonies and CFUs/ml were reduced, reaching a highest inhibition in CFU/ml at exposure time of 20 minutes, i.e. a dose of 120J/cm2 , with irradiance of 0.1 watt/ cm2. No significant reduction was recorded in CFU/ml at exposure time of 4 min (a dose of 24J/cm2). As a conclusion, the blue laser irradiation at wavelength of 450 nm and 500mw had antibacterial effects on P. mirabilis isolated from burn wounds with irradiance of 0.1watt/cm2 in vitro, as evidenced by the effective reduction in the viability of bacteria at a dose of 120J/cm2 corresponding to exposure time of 20 minutes.

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ANTIBACTERIAL EFFECTIVITY OF FLAVONOID FRACTION OF RAMANIA LEAF EXTRACT (Bouea macrophylla Griffith) AGAINST Porphyromonas gingivalis

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v6i1.10644 ◽

2021 ◽

Vol 6 (1) ◽

pp. 72

Author(s):

Lailatul Qomariyah ◽

Fransiska Uli Arta Panjaitan

Keyword(s):

Periodontal Disease ◽

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Leaf Extract ◽

Antibacterial Properties ◽

Chlorhexidine Gluconate ◽

Control Group ◽

Dilution Method ◽

Control Group Design ◽

Flavonoid Fraction

ABSTRACTBackground: Chronic periodontitis is a periodontal disease with 80% of all cases of periodontitis. The major causes are the accumulation of plaque and bacteria. The dominant bacteria in chronic periodontitis is Porphyromonas gingivalis. Treatment of chronic periodontitis can be done by scaling and root planing and supporting therapy by using mouthwash such as Chlorhexidine gluconate 0.2% which is the gold standard in the treatment of periodontal disease. Chlorhexidine gluconate 0.2% has disadvantages so that nowadays research on herbal plants is being done to find alternative medicines that are more effective. Ramania (Bouea machropylla Griffith) leaf contains flavonoids that have antibacterial properties. Objective: To analyze the antibacterial effectivity of the flavonoid fraction of Ramania leaf extract against Porphyromonas gingivalis that causes chronic periodontitis. Method: True experimental study and post-test with control group design consisting of 5 treatment groups, namely flavonoid fraction of ramania leaf extract with concentrations of 0.1%, 0.3%, and 0.5%, chlorhexidine gluconate 0.2% as a control positive and aquadest as a negative control. Each group was repeated 6 times. Antibacterial tests using the dilution method with inhibitory rates calculated using a UV-Vis spectrophotometer and killing rates were calculated using a Colony Counter. Results: The average difference in absorbance values obtained inhibitory rates at concentrations of 0.1%, 0.3%, and 0.5%. One Way Anova Test showed a significance value of 0,000 (p < 0.05). The average number of colonies after 24 hours incubation showed the results of a kill rates in the concentration group of 0.3%, 0.5%, and positive control. The Kruskal Wallis test showed a significance value of 0,000 (p < 0.05). Conclusion: The minimum inhibitory concentration (MIC) was obtained at a concentration of 0.1% and the minimum bactericidal concentration (MBC) was obtained at 0.3% concentration.Keywords: Flavonoid Fraction, MBC, MIC, Porphyromonas Gingivalis, Ramania Leaf Extract.

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Daya Antibakteri Ekstrak Daun Seledri (Apium graveolens L.) terhadap Porphyromonas gingivalis

STOMATOGNATIC - Jurnal Kedokteran Gigi ◽

10.19184/stoma.v16i2.23092 ◽

2019 ◽

Vol 16 (2) ◽

pp. 53

Author(s):

Anisa Luthfiyani ◽

Peni Pujiastuti ◽

Melok Aris W.

Keyword(s):

Porphyromonas Gingivalis ◽

Leaf Extract ◽

Antibacterial Effect ◽

Test Method ◽

Apium Graveolens ◽

Diffusion Method ◽

Antibacterial Test

Background: celery leaves contain antibacterial substances that can be used as medicine. These contents include flavonoids, saponins and tannins. Purpose: the aim of this study was to determine the antibacterial effect of celery leaves (Apium graveolens L.) on the growth of Porphyromonas gingivalis (P. gingivalis). Methods: celery leaves were extracted by maceration method with 96% ethanol. The antibacterial test method against P. gingivalis using the well diffusion method on BHI-A media. The concentrations of celery leaf extract used in this study were 6.25%, 12.5%, 25%, 50% and 100%. Result: The results showed that celery leaves extract (Apium graveolens L.) didn’t have the ability to inhibit the growth of Porphyromonas gingivalis. Conclusion: celery leaves didn’t have antibacterial effect against Porphyromonas gingivalis in 6,25%, 12.5%, 25%, 50% and 100% concentrations.

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The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

Bacterial Empire ◽

10.36547/be.2019.2.1.19-25 ◽

2019 ◽

Vol 2 (1) ◽

pp. 19

Author(s):

Murni Halim

Keyword(s):

Leaf Extract ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Natural Drugs ◽

Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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In Vitro Antimicrobial Activity of Zodia (Evodia suaveolens) Leaf Extract on Pathogenic Agents Dragon Fruit Plant

Jurnal Biota ◽

10.19109/10.19109/biota.v6i2.6236 ◽

2020 ◽

Vol 6 (2) ◽

pp. 78-85

Author(s):

Trio Ageng Prayitno ◽

Nuril Hidayati

Keyword(s):

Antimicrobial Activity ◽

Leaf Extract ◽

Plant Pathogens ◽

Inhibition Zone ◽

Diffusion Method ◽

Leaf Extracts ◽

Dragon Fruit ◽

Fruit Plants ◽

Pathogenic Agents

The use of antimicrobials from plant extracts has not been used optimally to control pathogenic agents in dragon fruit plants. The purpose of this research was to determine the antimicrobial activity of zodia (Evodia suaveolens) leaf extracts on pathogenic agents of dragon fruit plants in vitro. The research method is laboratory research with Completely Randomized Design (CRD). The antimicrobial concentrations of zodia (E. suaveolens) leaf extract used six types including 50%, 60%, 70%, 80%, 90%, and 100% with four replications. The research sample was the leaf of zodia (E. suaveolens), Pseudomonas aeruginosa and Fusarium oxysporum strain Malang. Test the antimicrobial activity of zodia (E. suaveolens) leaf extracts on the growth of P. aeruginosa and F. oxysporum using the disc-diffusion method and wells method. The research instrument was used the observation sheet of the diameter of inhibition zone indicated by the clear zone. The diameter of inhibition zone data were analyzed using the One Way ANOVA test. The results showed that the antimicrobial activity of zodia (E. suaveolens) leaf extract significantly inhibited the growth of P. aeruginosa and F. oxysporum (P <0.05). These results recommend zodia (E. suaveolens) leaf extract as an antimicrobial agent for dragon fruit plant pathogens.

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