scholarly journals Induction of lymphocyte apoptosis in healthy individuals and patients with rheumatoid arthritis under “cellular neighborhood” in vitro

2019 ◽  
Vol 18 (1) ◽  
pp. 155-163
Author(s):  
T. Ya. Abramova ◽  
V. A. Tsura ◽  
E. A. Blinova ◽  
A. Yu. Morenkova ◽  
O. A. Chumasova ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Lymphocyte ◽  
Culture Media ◽  
Living Cells ◽  
Lymphocyte Apoptosis ◽  
Healthy Individuals ◽  
Physiological Conditions ◽  
Lymphocyte Cultures ◽  
Late Stages

The aim of the study was to investigate the features of T-lymphocyte apoptosis induced by components of autologous apoptotic cultures in vitro in norm and rheumatoid arthritis in the context of «cellular neighborhood».Materials and methods. Subjects of the study were blood samples of patients with rheumatoid arthritis (RA) and healthy women of comparable age. Developed protocol allowed to differentially evaluate the parameters of proliferation, early and late stages of apoptosis in the «primary» (CFSE-) and «secondary» (CFSE+) induced apoptotic T-lymphocyte cultures. It was estimated the effect of cellular and humoral components of unstimulated, anti-CD3- and dexamethasone-stimulated cells under the conditions of overcrowding and depleted culture media on autologous lymphocytes, cultured under physiological conditions, in norm and RA.Results. Comparative qualitative analysis revealed the features of the processes of T-lymphocyte apoptosis in norm and pathology. Also, the parameters of early and late stages of apoptosis of a «primary» induced culture and «secondary» induced cells after transferring the cellular and humoral components of apoptotic cultures did not differ significantly either initially or during culturing in both investigated groups. But it was a significant increase in the amount of living T-cells in «primary»-induced unstimulated and dexamethasone-stimulated RA patients’ cultures compared to similar donors’ cultures.Conclusion. There was no difference between stimulated with anti-CD3 antibodies cells and the «secondary» induced cultures. Taking into account the absence of significant differences in the parameters of activation apoptosis, the increased number of living cells in RA patients’ cultures relative to donors’ is evidence of contribution of non-autonomous apoptosis effects to cellular homeostasis in RA.

scholarly journals Condensed chromatin behaves like a solid on the mesoscale in vitro and in living cells

2020 ◽  
Author(s):  
Hilmar Strickfaden ◽  
Thomas Tolsma ◽  
Ajit Sharma ◽  
D. Alan Underhill ◽  
Jeffrey C. Hansen ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Living Cells ◽  
Physiological Conditions ◽  
Transmission Electron ◽  
Material State ◽  
Temporal And Spatial ◽  
Self Association ◽  
Eukaryotic Genomes

SUMMARYThe association of nuclear DNA with histones to form chromatin is essential to the temporal and spatial control of eukaryotic genomes. In this study, we examined the physical state of chromatin in vitro and in vivo. Our in vitro studies demonstrate that MgCl2-dependent self-association of native chromatin fragments or reconstituted nucleosomal arrays produced supramolecular condensates whose constituents are physically constrained and solid-like. Liquid chromatin condensates could be generated in vitro, but only using non-physiological conditions. By measuring DNA mobility within heterochromatin and euchromatin in living cells, we show that chromatin also exhibits solid-like behavior in vivo. Representative heterochromatin proteins, however, displayed liquid-like behavior and coalesced around a solid chromatin scaffold. Remarkably, both euchromatin and heterochromatin showed solid-like behavior even when transmission electron microscopy revealed limited interactions between chromatin fibers. Our results therefore argue that chromatin is not liquid but exists in a solid-like material state whose properties are tuned by fiber-fiber interactions.

Influence of Methylprednisolone on Late Stages of Differentiation of Memory CD4+ T Cells in Rheumatoid Arthritis: In Vitro Study

2019 ◽  
Vol 45 (3) ◽  
pp. 310-315
Author(s):  
N. M. Todosenko ◽  
K. A. Yurova ◽  
O. G. Khaziakhmatova ◽  
L. S. Litvinova
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Cd4 T Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Memory Cd4 T Cells ◽  
Late Stages

In Vitro Cellular Aging in T-Lymphocyte Cultures: Analysis of DNA Content and Cell Size

1993 ◽  
Vol 207 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Nancy L. Perillo ◽  
Faramarz Naeim ◽  
Roy L. Walford ◽  
Rita B. Effros
Keyword(s):  
Cell Size ◽  
T Lymphocyte ◽  
Dna Content ◽  
Cellular Aging ◽  
Lymphocyte Cultures

A Bio-Photonics System for Rapid In Vitro Testing of Cells and Ceramics

2005 ◽  
Vol 284-286 ◽  
pp. 531-536 ◽  
Author(s):  
Larry L. Hench ◽  
Ioan Notingher
Keyword(s):  
Cell Damage ◽  
Spectroscopic Method ◽  
Living Cells ◽  
In Vitro Cultures ◽  
Animal Testing ◽  
Time Analysis ◽  
Cellular Functions ◽  
Physiological Conditions ◽  
Real Time Analysis

We present a new bio-photonic method based on Raman spectroscopy able to characterize living cells in in-vitro cultures. The main advantages of this technology are: no labels or other contrast enhancers are required; provides real-time analysis; cells can be maintained in physiological conditions during the measurements; no cell-damage is induced during the measurements; it is rich in information about the biochemical composition of the cell. The results show that this spectroscopic method can be used to study the most important cellular functions involved in cell-biomaterial interactions, such as cell death, differentiation, de-differentiation and mineralization. The method offers the potential for studying cell-bioceramics interaction and reduce the need of animal testing until the final steps of proving efficacy prior to clinical trials.

scholarly journals Melatonin Prevents T Lymphocyte Infiltration to the Kidneys of Hypertensive Rats, Induced by a High-Salt Diet, by Preventing the Expression of CXCR3 Ligand Chemokines

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3577
Author(s):  
Ariel Bier ◽  
Rawan Khashab ◽  
Yehonatan Sharabi ◽  
Ehud Grossman ◽  
Avshalom Leibowitz
Keyword(s):  
Mrna Expression ◽  
T Lymphocyte ◽  
Mesangial Cells ◽  
Culture Media ◽  
High Salt ◽  
Lymphocyte Infiltration ◽  
High Salt Diet ◽  
Salt Diet ◽  
Isolation And Characterization

In a previous study, we demonstrated that melatonin prevents kidney damage in a salt-induced hypertension model by decreasing oxidative stress. We hypothesized that this effect involves melatonin’s immunomodulatory properties. In vivo Study-Dahl salt-sensitive (DSS) rats were fed normal chow, a high-salt diet (HSD), or a HSD and melatonin (30 mg/kg/day) in their water for eight weeks. Kidneys were harvested for immediate lymphocyte isolation and characterization by Flow cytometry (CD3+CD4+ and CD3+CD8+) and for lymphocyte chemoattractant (mainly CXCL chemokines) gene expression studies. In vitro study-rat mesangial cells (RMC) were cultured in a high-salt medium without and with melatonin. A HSD was associated with significant renal infiltration of CD4+ and CD8+ T lymphocytes compared to control. Melatonin significantly reduced renal lymphocyte infiltration. A HSD significantly increased mRNA expression of CXCL chemokines. Adding melatonin to the HSD abolished this effect. Treating RMC cells with salt increased the expression of CXCL10 and CXCL11 but not CXCL9. Adding melatonin to the culture media prevented this increase. Treating HSD-fed rats with melatonin decreased renal lymphocyte chemoattractant mRNA expression and is associated with significantly reducing renal T lymphocyte infiltration. Salt may have a direct effect on chemokine-producing renal cells, which is blunted by melatonin treatment.

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