scholarly journals Comparison of cutting and coagulation properties of 1.56 and 1.94 µm fiber lasers and a 0.98 µm semiconductor laser

2022 ◽  
Vol 20 (4) ◽  
pp. 56-62
Author(s):  
M. A. Ryabova ◽  
M. Yu. Ulupov ◽  
N. A. Shumilova ◽  
G. V. Portnov ◽  
E. K. Tikhomirova ◽  
...  
Keyword(s):  
Semiconductor Laser ◽  
Fiber Lasers ◽  
Radiation Power ◽  
Biological Effects ◽  
Laser Wavelength ◽  
Ablation Zone ◽  
Tissue Samples ◽  
Zone Depth ◽  
Laser Incision ◽  
Coagulation Zone

Aim of the study was to compare the cutting and coagulation properties of 1.56 and 1.94 μm fiber lasers with those of a 0.98 μm semiconductor laser.Materials and methods. A comparative study of the biological effects of 1.56 and 1.94 µm lasers and a 0.98 µm semiconductor laser used in a constant, continuous mode was carried out. The cutting properties of the lasers were evaluated on the chicken muscle tissue samples by the width and depth of the ablation zone formed via a linear laser incision at a speed of 2 mm/s, while the coagulation properties were assessed by the width of the lateral coagulation zone. The zones were measured using a surgical microscope and a calibration slide. For statistical analysis, power values of 3, 5, 7, 9, and 11 W were chosen for each laser wavelength.Results. Analysis of the findings confirmed that laser wavelength had a statistically significant effect on the linear dependence between incision parameters and laser power. It was found that the 1.56 μm fiber laser (water absorption) had a greater coagulation ability but a comparable cutting ability compared with the 0.98 μm laser (hemoglobin absorption). When used in the power mode of 7W or higher, the 1.94 µm laser provided superior cutting performance compared with the 0.98 µm semiconductor laser at the same exposure power. Elevating the power in any of the lasers primarily increased the width of the ablation zone, and to a lesser extent – the crater depth and the width of the lateral coagulation zone. Therefore, in comparison with the 0.98 μm semiconductor laser, higher radiation power in the 1.56 and 1.94 μm lasers mainly influences their cutting properties, expanding the width and depth of the ablation zone, and has a smaller effect on their coagulation ability.Conclusion. The findings of the study showed that the 1.56 and 1.94 μm fiber lasers have better coagulation properties in comparison with the 0.98 μm semiconductor laser. was statistically proven that all incision characteristics (width of the lateral coagulation zone, depth and width of the ablation zone) for the 1.56, 1.94, and 0.98 μm lasers depend on the power of laser radiation. The 1.94 µm laser is superior to the 0.98 µm laser in its cutting properties. 

Tailoring the output laser wavelength of fiber lasers by the intra-cavity inscription of LPFGs

2016 ◽  
Vol 58 (6) ◽  
pp. 1430-1433
Author(s):  
R.E. Nuñez-Gomez ◽  
G. Anzueto-Sánchez ◽  
A. Martínez-Rios ◽  
I. Torres-Gomez ◽  
J. Camas-Anzueto ◽  
...  
Keyword(s):  
Fiber Lasers ◽  
Laser Wavelength ◽  
Output Laser

Self-sweeping of laser wavelength and associated mode instabilities in fiber lasers

2017 ◽  
Author(s):  
Pavel Peterka ◽  
Pavel Honzatko ◽  
Jan Aubrecht ◽  
Petr Navratil ◽  
Pavel Koska ◽  
...  
Keyword(s):  
Fiber Lasers ◽  
Laser Wavelength

Coherent tuning of semiconductor laser wavelength

2006 ◽  
Vol 45 (1) ◽  
pp. 014202
Author(s):  
Sahnggi Park
Keyword(s):  
Semiconductor Laser ◽  
Laser Wavelength

scholarly journals Down-regulation of LAMP3 inhibits the proliferation and migration of HSCC

2021 ◽  
Author(s):  
Xiaoxia Qiu ◽  
Juanjuan Li ◽  
Keke Yang ◽  
Song Jiang ◽  
Yong Yin ◽  
...  
Keyword(s):  
Biological Effects ◽  
Hypopharyngeal Cancer ◽  
Down Regulation ◽  
Tissue Samples ◽  
Protein Levels ◽  
Fadu Cells ◽  
And Migration ◽  
Proliferation Assays

Abstract BackgroundLysosomal-associated membrane glycoprotein 3 (LAMP3) has been shown to be highly expressed in various types of tumors. It is associated with their poor prognosis, proliferation, invasion, and metastasis, respectively. However, the role of LAMP3 in hypopharyngeal squamous cell carcinoma (HSCC) is unclear.ObjectiveThis article aims to investigate the role of LAMP3 in the proliferation and metastasis of HSCC.MethodDetection of the expression of LAMP3 in clinical HSCC and paired adjacent healthy tissue samples by using immunohistochemistry. Furthermore, LAMP3 was knocked out of the HSCC cell line, FaDu, using a lentivirus vector and the in vitro biological effects of LAMP3 knockdown in FaDu cells were studied.ResultsImmunohistochemistry results showed that LAMP3 was highly expressed in HSCC. Additionally, it was verified at the mRNA and protein levels that lentiviral transduction effectively down-regulated the expression of LAMP3 in FaDu cells. Cell colony formation assays and CCK8 proliferation assays showed that down-regulation of LAMP3 on FaDu cells inhibited cell proliferation and growth rate. Wound healing experiments and transwell experiments showed that significant down-regulation of LAMP3 on FaDu cells inhibited cell migration.ConclusionCollectively, our results suggest that LAMP3 is a useful therapeutic target for the treatment of hypopharyngeal cancer.

scholarly journals The origins and functional effects of postzygotic mutations throughout the human lifespan

2021 ◽  
Author(s):  
Nicole Briana Rockweiler ◽  
Avinash Ramu ◽  
Liina Nagirnaja ◽  
Wing Hing Wong ◽  
Michiel J. Noordam ◽  
...  
Keyword(s):  
Biological Effects ◽  
Phylogenetic Reconstruction ◽  
Tissue Expression ◽  
Prenatal Development ◽  
Tissue Samples ◽  
Human Lifespan ◽  
Specific Effects ◽  
Mutation Burden ◽  
Functional Consequences ◽  
Postzygotic Mutations

Postzygotic mutations (PZMs) begin to accrue in the human genome immediately after fertilization, but how and when PZMs affect development and lifetime health remains unclear. To study the origins and functional consequences of PZMs, we generated a multi-tissue atlas of PZMs from 948 donors using the final major release of the Genotype-Tissue Expression (GTEx) project. Nearly half the variation in mutation burden among tissue samples can be explained by measured technical and biological effects, while 9% can be attributed to donor-specific effects. Through phylogenetic reconstruction of PZMs, we find that their type and predicted functional impact varies during prenatal development, across tissues, and the germ cell lifecycle. Remarkably, a class of prenatal mutations was predicted to be more deleterious than any other category of genetic variation investigated and under positive selection as strong as somatic mutations in cancers. In total, the data indicate that PZMs can contribute to phenotypic variation throughout the human lifespan, and, to better understand the relationship between genotype and phenotype, we must broaden the long-held assumption of one genome per individual to multiple, dynamic genomes per individual.

Long non-coding RNA LINC00115 contributes to the progression of colorectal cancer by targeting miR-489-3p via PI3K/AKT/mTOR pathway

2020 ◽  
Author(s):  
Weiyu Feng ◽  
Baodong Li ◽  
Jinbang Wang ◽  
Huiliang Zhang ◽  
Yonggang Liu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Inverse Correlation ◽  
Mtor Signaling ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Regulatory Relationship ◽  
Tissue Samples

Abstract Background Long noncoding RNAs (lncRNAs) are tumor-related regulators and have been found to be involved in the underlying molecular mechanisms of colorectal cancer (CRC). However, the role of lncRNA LINC00115 during CRC progression is not entirely elucidated. Methods The expression of LINC00115 was analyzed in paired CRC tissue samples and its clinical significance was evaluated. The biological effects on CRC cells proliferation, apoptosis, migration, invasion and PI3K/AKT/mTOR signaling were assessed by Cell Counting Kit-8 assay, Transwell assay, flow cytometry analysis and Western blot, respectively. The regulatory relationship between LINC00115 and miR-489-3p was determined by dual-luciferase reporter assays. Results LINC00115 was significantly overexpressed in CRC and its overexpression predicted poor outcome of the patients. Downregulation of LINC00115 markedly inhibited CRC cell proliferation, increased cell apoptosis, and suppressed cell migration and invasion. Moreover, downregulation of LINC00115 led to the inactivation of PI3K/AKT/mTOR signaling. Bioinformatics analysis identified miR-489-3p as a candidate target of LINC00115. Furthermore, we revealed an inverse correlation between LINC00115 and miR-489-3p in CRC tissues. miR-489-3p might directly target LINC00115 and downregulation of miR-489-3p could rescue the biological effects induced by the absence of LINC0015. Conclusion LINC00115 serves as an excellent oncogene of CRC metastasis, the deeper understanding of LINC00115/miR-489-3p axis might provide potential therapeutic targets for CRC metastasis.

Biological effects of semiconductor laser irradiation on peanut seeds

2002 ◽  
Author(s):  
Lin Ou ◽  
Rong Chen ◽  
Tong Lin ◽  
Huashang Xiao ◽  
Yajue Zhen
Keyword(s):  
Semiconductor Laser ◽  
Laser Irradiation ◽  
Biological Effects

Phase II clinical study on intraoperative photodynamic therapy with talaporfin sodium and semiconductor laser in patients with malignant brain tumors

2013 ◽  
Vol 119 (4) ◽  
pp. 845-852 ◽  
Author(s):  
Yoshihiro Muragaki ◽  
Jiro Akimoto ◽  
Takashi Maruyama ◽  
Hiroshi Iseki ◽  
Soko Ikuta ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Brain Tumors ◽  
Semiconductor Laser ◽  
Positive Impact ◽  
Radiation Power ◽  
Combined Treatment ◽  
Tumor Control ◽  
Study Cohort ◽  
Newly Diagnosed ◽  
Talaporfin Sodium

Object The objective of the present study was to perform a prospective evaluation of the potential efficacy and safety of intraoperative photodynamic therapy (PDT) using talaporfin sodium and irradiation using a 664-nm semiconductor laser in patients with primary malignant parenchymal brain tumors. Methods In 27 patients with suspected newly diagnosed or recurrent primary malignant parenchymal brain tumors, a single intravenous injection of talaporfin sodium (40 mg/m2) was administered 1 day before resection of the neoplasm. The next day after completion of the tumor removal, the residual lesion and/or resection cavity were irradiated using a 664-nm semiconductor laser with a radiation power density of 150 mW/cm2 and a radiation energy density of 27 J/cm2. The procedure was performed 22–27 hours after drug administration. The study cohort included 22 patients with a histopathologically confirmed diagnosis of primary malignant parenchymal brain tumor. Thirteen of these neoplasms (59.1%) were newly diagnosed glioblastomas multiforme (GBM). Results Among all 22 patients included in the study cohort, the 12-month overall survival (OS), 6-month progression-free survival (PFS), and 6-month local PFS rates after surgery and PDT were 95.5%, 91%, and 91%, respectively. Among patients with newly diagnosed GBMs, all these parameters were 100%. Side effects on the skin, which could be attributable to the administration of talaporfin sodium, were noted in 7.4% of patients and included rash (2 cases), blister (1 case), and erythema (1 case). Skin photosensitivity test results were relatively mild and fully disappeared within 15 days after administration of photosensitizer in all patients. Conclusions Intraoperative PDT using talaporfin sodium and a semiconductor laser may be considered as a potentially effective and sufficiently safe option for adjuvant management of primary malignant parenchymal brain tumors. The inclusion of intraoperative PDT in a combined treatment strategy may have a positive impact on OS and local tumor control, particularly in patients with newly diagnosed GBMs. Clinical trial registration no.: JMA-IIA00026 (https://dbcentre3.jmacct.med.or.jp/jmactr/App/JMACTRS06/JMACTRS06.aspx?seqno=862).

scholarly journals RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Xin Wang ◽  
Linli Tian ◽  
Yushan Li ◽  
Jingting Wang ◽  
Bingrui Yan ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Biological Effects ◽  
Tissue Samples ◽  
Downstream Target ◽  
Transcriptomic Sequencing ◽  
Rna Immunoprecipitation ◽  
M6a Rna Methylation ◽  
Hoechst Staining

Abstract Background Laryngeal cancer has the highest mortality rate among head and neck tumours. RNA N6-methyladenosine (m6A) is the most plentiful and variable in mammalian mRNA. Yet, the m6A regulatory mechanism underlying the carcinogenesis or progression of LSCC remains poorly understood. Methods The m6A RNA methylation quantification kit was used to detect tissue methylation levels. m6A microarray analysis, mRNA transcriptomic sequencing (mRNA-seq), and proteomics were used to determine RBM15, TMBIM6, and IGF2BP3. Immunohistochemical (IHC), quantitative real-time PCR (qRT-PCR) and Western blot were used to investigate RBM15, TMBIM6, and IGF2BP3 expression in tissue samples and cell lines. The biological effects of RBM15 were detected both in vitro and in vivo. The combination relationship between RBM15/IGF2BP3 and TMBIM6 was verified by RNA immunoprecipitation (RIP) assay, Methylated RNA immunoprecipitation sequencing (MeRIP-seq), RNase Mazf, and luciferase report assay. RNase Mazf was used to determine the methylation site on TMBIM6 mRNA. Hoechst staining assay was used to confirm the apoptotic changes. The actinomycin D verified TMBIM6 stability. Results The global mRNA m6A methylation level significantly increased in LSCC patients. RBM15, as a “writer” of methyltransferase, was significantly increased in LSCC and was associated with unfavorable prognosis. The knockdown of RBM15 reduced the proliferation, invasion, migration, and apoptosis of LSCC both in vitro and in vivo. The results were reversed after overexpressing RBM15. Mechanically, TMBIM6 acted as a downstream target of RBM15-mediated m6A modification. Furthermore, RBM15-mediated m6A modification of TMBIM6 mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Conclusion Our results revealed the essential roles of RBM15 and IGF2BP3 in m6A methylation modification in LSCC, thus identifying a novel RNA regulatory mechanism.

scholarly journals Comparison of efficacy of laser and radio-wave methods of surgical treatment of patients with chronic rhinitis

2021 ◽  
pp. 116-122
Author(s):  
V. M. Svistushkin ◽  
E. S. Shchennikova ◽  
E. V. Sinkov
Keyword(s):  
Semiconductor Laser ◽  
Clinical Symptoms ◽  
Healing Process ◽  
Life Quality ◽  
Laser Wavelength ◽  
Drug Induced ◽  
Chronic Rhinitis ◽  
Wave Methods ◽  
Turbinate Reduction

Introduction. Chronic rhinitis occurs more often in patients; it’s symptoms deteriorate their quality of life, affect sleeping, daily activity, and sometimes cause complications. The treatment depends on the clinical symptoms. In case of inefficiency of conservative therapy, surgery is indicated. Choosing an exact surgical instrument is still controversial.Objective. The aim of our study is to compare using semiconductor laser (wavelength 1.56 mkm) and radiofrequency (4 MHz) in patients with chronic rhinitis.Materials and methods. 60 patients with chronic drug-induced rhinitis aged 19–80 years were recruited and divided into two equal groups. All patients underwent surgical procedures: semiconductor laser (IRE-Polus) and radiofrequency (Ellman Surgitron) inferior nasal turbinate reduction. To assess and compare the healing process in two groups we used endoscopy and active anterior rhinomanometry. All patients filled out the SNOT-20 questionnaire. For the mucociliary clearance evaluation saccharin test was used. We observed patients during 12 months.Conclusion. Both, laser and radiofrequency, techniques are effective in patients with chronic rhinitis. In laser group shorter healing period was observed, what leads to rehabilitation shortening and life quality improvement. 

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