scholarly journals Evaluation of Formulations through Testing the Bioefficacy of Selected Trichoderma Isolate against S. rolfsii

2020 ◽  
Vol 9 (9) ◽  
pp. 301-309
Author(s):  
B. Swathi ◽  
A. K. Patibanda ◽  
J. Krishna Prasadji ◽  
P. V. Krishnayya ◽  
M. Lal Ahamed
Keyword(s):  
Trichoderma Isolate

scholarly journals Inhibitory Effect of Trichoderma asperellum Isolate against Ralstonia solanacearum Causing Brinjal Wilt

2021 ◽  
Vol 24 (2) ◽  
pp. 107-120
Author(s):  
SMN Islam ◽  
SS Siddique ◽  
MZH Chowdhury ◽  
NJ Mishu
Keyword(s):  
Ralstonia Solanacearum ◽  
Disease Incidence ◽  
Its Region ◽  
Inhibitory Effect ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
In Planta ◽  
Quantitative Test ◽  
Trichoderma Isolate

A native Trichoderma isolate was collected from the agricultural soil of Gazipur. This isolate was identified as a Trichoderma asperellum through morphology and analysis of internal transcribed spacer (ITS) region of ribosomal RNA gene sequence and reconstruction of the phylogenetic tree. The antagonistic effects of the newly identified T. asperellum isolate were assessed against brinjal bacterial wilt caused by Ralstonia solanacearum both in vitro and in planta. Both qualitative and quantitative bioassays were conducted in vitro. For qualitative tests, dual culture and antibacterial activity were carried out, and pathogen growth was observed visually. The antagonism of T. asperellum cell free culture filtrate on the growth of R. solanacearum was conducted in a quantitative test. Successful antagonism was recorded after both in vitro qualitative tests. In addition, the lowest colony forming unit was recorded in 100% of CFC (2.4±0.51 ×103 cfu/ml) in quantitative test. The T. asperellum inoculated plant showed low disease incidence (13.33%) when seedlings were challenged with R. solanacearum in planta experiment. Disease incidence was 100% for seedlings when treated with only R. solanacearum. The results showed that the isolated and identified T. asperellum isolate suppressed R. solanacearum growth in vitro and protected the seedling from wilting in planta. Therefore, this isolate could be considered as a potential isolate. Ann. Bangladesh Agric. (2020) 24(2) : 107-120

scholarly journals Trichoderma Contributes to the Germination and Seedling Development of Açaí Palm

Agriculture ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 456
Author(s):  
Bruno Fróes Campos ◽  
Anselmo Junior Corrêa Araújo ◽  
Cristina Aledi Felsemburgh ◽  
Thiago Almeida Vieira ◽  
Denise Castro Lustosa
Keyword(s):  
Dry Mass ◽  
Initial Growth ◽  
Randomized Design ◽  
Trichoderma Isolate ◽  
Number Of Leaves ◽  
Collar Diameter ◽  
Completely Randomized Design ◽  
Set Up ◽  
Root Size ◽  
Trichoderma Isolates

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.

scholarly journals Pellet Formulation of Trichoderma Isolated from Aceh with Potential for Biocontrol of Phytophthora Sp. on Cacao Sedlings

2019 ◽  
Vol 3 (2) ◽  
pp. 202-212
Author(s):  
Rina Sriwati ◽  
Tjut Chamzhurni ◽  
Alfizar Alfizar ◽  
Bonny PW Soekarno ◽  
Vina Maulidia ◽  
...  
Keyword(s):  
Biological Control ◽  
Disease Severity ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Diameter Growth ◽  
Good Ability ◽  
Trichoderma Isolate ◽  
Pellet Formulation

Molleculler study was conducted to identify several species of Trichoderma isolate from several  plant (Pine, Cacao, Gliceria, Nutmeg, Bamboo, Coffee, Potato).  The growth of eight species Trichodermaafter pelleting formulation has been observed. Pellet Trichoderma harzianum have good ability to growth on PDA medium after 4 weeks storage. Base on their mycelium diameter growth on PDA, T. harzianum have selected as potential species on pellet formulation growth.  Several dose of pellet formulation have been applied for controlling Phythopthora disease. The application of T. harzianum pellets in the form of a 2 g / 100 ml (S1) suspension effective in inhibiting the development of Phytophthora sp in cacao seedlings, when the higher concentrations of T. harzianum pellets applied to cacao seeds,the disease severity increase. Pellet Trichoderma could be use as biological control agent of cacao seedling in certain dosage.

scholarly journals Screening of Fungi from Oil Palm Rhizosphere in Peat Soils and the Potential as Biological Agents against Ganoderma boninense

2019 ◽  
Vol 2 (2) ◽  
pp. 37-49
Author(s):  
Fifi Puspita ◽  
Isna Rahma Dini ◽  
Dermala Sari
Keyword(s):  
Oil Palm ◽  
Peat Soil ◽  
Morphological Characteristics ◽  
Severity Index ◽  
Biological Agents ◽  
Ganoderma Boninense ◽  
Peat Soils ◽  
Rhizosphere Fungi ◽  
Randomized Design ◽  
Trichoderma Isolate

 One of the diseases that attack oil palm plants is stem rot disease. Control efforts that can be done is to use rhizosphere fungi from oil palm plants in peat soils. This study aimed to select fungi from rhizosphere of oil palm plants in peat soil based on morphological characteristics and test their potential as biological agents against Ganoderma boninense. This research was conducted by exploration, observation and experiment by using complete randomized design (RAL). The parameters observed were macroscopic characteristics of fungi from oil palm rhizosphere, disease severity index, fungus inhibition power from oil palm rhizosphere to G. boninense, colony diameter and growth rate of high antagonist rhizosphere fungus, hyperparasitic type of fungus from rhizosphere of oil palm plant with G. boninense and the morphological characteristics of fungi from high antagonist rhizosphere in macroscopic and microscopic. The results showed that 12 rhizosphere fungi isolates and 4 isolates were antagonist to G. boninense. Isolate J5 has a high antagonist power of 70.26% and is a genus Trichoderma, isolate J7 belongs to the genus Trichoderma, isolate J10 genus Aspergillus and isolate J12 genus Mucor.

Application Test Rhizobium and Trichoderma To The Growth and Yield Of Soybean (Glycine Max (L))

Nabatia ◽  
2017 ◽  
Vol 5 (1) ◽  
pp. 55-62
Author(s):  
Sutarman Sutarman ◽  
Meilani Fatimah
Keyword(s):  
Plant Root ◽  
Root Nodules ◽  
Leaf Age ◽  
Growth And Yield ◽  
Mimosa Pudica ◽  
Factor Ii ◽  
Two Factors ◽  
Glycine Max L ◽  
Trichoderma Isolate ◽  
Wet Weight

The objective of the research was to know of the responses of the aplication Rhizobium of nodule root Soybean as well as nodule root Mimosa pudica and Trichoderma iso- late Tc-Jjr02. The research was conducted on the Green House Faculty of Agriculture, Muhammadiyah University of Sidoarjo, from Januari to Mei 2016. This research com- piled using a factorial randomized complete design which consists of 2 factors : factor I is application Rhizobium consisting of : R0 (withow Rhizobium), R1(Rhizobium of soy- bean), R2(Rhizobium of mimosa pudica). Factor II application Trichoderma consisting of : T0 (withow Trichoderma) and T1 (Trichoderma isolat Jati Jejer-02). Of these two factors obtained 6 treatment combinations and repeated 4 times. From the analysis of statisti- cal data shows that application Rhizobium on significant effect the amount of leaf age 40 HST and 60 HST as well as the number of pods, planting seed weight , weight of 100 seeds, and fresh weight of plant root nodules. Application Rhizobium significantly affected the wet weight of stover. Trichoderma isolate Jati Jejer-02 capable symbio- sis with the plant so that the very significant effect on plant height age 7 HST and the amount of leaf age 20 HST. As well as the interaction between application Rhizobium and Trichoderma isolates significantly affected the number of pods plant.

Competition between Phytophthora cinnamomi and Trichoderma spp. in autoclaved soil

1976 ◽  
Vol 22 (8) ◽  
pp. 1120-1127 ◽  
Author(s):  
W. D. Kelley ◽  
R. Rodriguez-Kabana
Keyword(s):  
Phytophthora Cinnamomi ◽  
Trichoderma Spp ◽  
Trichoderma Isolate

Results from analyses of β-glucosidase (EC 3.2.1.21) and phosphatase (EC3.1.3.1; EC 3.1.3.2) activities indicated that presence of a Trichoderma isolate reduced development of Phytophthora cinnamomi. It was also observed that P. cinnamomi was more competitive in coinoculated cultures than in cultures where Trichoderma was added on day 3. Analysis of trehalase (EC 3.2.1.28) activity indicated that Trichoderma either utilized portions of the P. cinnamomi mycelium as substrate or the action of P. cinnamomi released additional nutrients not normally available to Trichoderma. The stronger Trichoderma isolate was T. harzianum.

scholarly journals In vitro CONFRONTATION OF Trichoderma spp. ISOLATES WITH PHYTOPATHOGENIC AND NON-PHYTOPATHOGENIC FUNGI

2016 ◽  
Vol 3 (2) ◽  
pp. 1-8 ◽  
Author(s):  
Milton Luiz da Paz Lima ◽  
Maria Cristina Araújo Vaz ◽  
Aline Suelen da Silva ◽  
Karoliny De Almeida Souza ◽  
Gabriel Isaias Lee Tuñon
Keyword(s):  
Filamentous Fungi ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Trichoderma Spp ◽  
Opposite Position ◽  
Trichoderma Isolate ◽  
Alternaria Sp ◽  
Area Percentage

The aim of this study was the confrontation of Trichoderma spp. with pathogenic and non-pathogenic fungi. The Trichoderma spp. and filamentous fungi (Fusarium solani, Alternaria sp., Colletotrichum gloeosporioides, and Aspergillus niger) were transferred to potato-dextrose-agar (PDA). The confrontation was performed using Petri dishes inoculated with mycelial disks in opposite position (9 mm) of Trichoderma spp. and filamentous fungi. After inoculation the plates remained under 25 ° C regime to 12 hours light for a period of seven days. After this period was evaluated qualitatively according to the scale of Bell et al. (1982), which consists of the suitability scores for the area percentage of the culture medium under expression of antagonism. The isolate of C. gloeosporioides (jatropha) was isolated which had statistically slightly inhibited growth by Trichoderma spp., Unlike the isolated Alternaria sp. (parsley) was isolated micelial fungi suffered the most antagonism. Statistically the Trichoderma isolate derived from pineapple promoted the highest antagonistic activity against isolates of pathogenic fungi tested, being a candidate for use in biocontrol.

scholarly journals EVALUASI KOMBINASI ISOLAT TRICHODERMA MIKOPARASIT DALAM MENGENDALIKAN PENYAKIT AKAR PUTIH PADA BIBIT KARET

2011 ◽  
Vol 8 (1) ◽  
pp. 52-55
Author(s):  
Suwandi Suwandi
Keyword(s):  
Disease Severity ◽  
Root Disease ◽  
Disease Suppression ◽  
Trichoderma Virens ◽  
Biocontrol Efficacy ◽  
Rubber Wood ◽  
Trichoderma Isolate ◽  
Rigidoporus Lignosus

Evaluation of mycoparasitic Trichoderma isolate mixtures to control white root disease on rubber seedlings.  Eight isolates of mycoparasitic Trichoderma, as single cultures or in isolate mixtures were tested for their biocontrol efficacy against rubber seedlings inoculated with one of three strains of Rigidoporus lignosus. Biocontrol efficacy of isolates was varied, but not significantly affected by strains of R. lignosus. Mixtures of four isolates were significantly (P < 0.05) reduced the disease severity and percentage of root necrotic as compared to mixtures of two isolates as well as single isolates.  The highest disease suppression (65% relative to control) and reduction of inocula on rubber wood sticks (91% relative to control) was achieved in four isolate mixtures of Trichoderma virens (T1+T4+ T9+ T11).

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