scholarly journals Influence of Different Vegetable Oils on In Vitro Ruminal Fermentability and Nutrient Digestibility in Ettawah Crossbred Goat

2020 ◽  
Vol 21 (1) ◽  
pp. 22
Author(s):  
Anis Muktiani ◽  
Nurul Arifah ◽  
Widiyanto Widiyanto
Keyword(s):  
Vegetable Oil ◽  
Palm Oil ◽  
Seed Oil ◽  
Corn Oil ◽  
Rumen Fluid ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

This research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments.

scholarly journals The Effect of Total or Partial Protected Vegetable Oil Supplementation on In Vitro Digestibility, Feed Fermentability and Energy Efficiency

2019 ◽  
Vol 43 (3) ◽  
Author(s):  
Laily Ismatul Faizah ◽  
Widiyanto Widiyanto ◽  
Anis Muktiani
Keyword(s):  
Energy Efficiency ◽  
Palm Oil ◽  
Methane Production ◽  
Corn Oil ◽  
Block Design ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Dry Matter Digestibility ◽  
Ch4 Production

This study was examines the effect of oil (oil palm and corn) combination with the level of protection (total or partial) on feed fermentability, methane production and energy efficiency with in vitro techniques. The experiment was designed using a factorial pattern 2x2, factor A = type of oil (corn and palm) and factor B = oil protection level (total and partial), each treatment combination was repeated 4 times. Data was processed by analyzing various factorial patterns in a randomized block design and if there was an influence between treatments performed by the Dunca test. The results showed that there was no interaction effect (P>0.05) between the types of oil with the level of protection in all parameters, except NH3. Supplementation of palm oil produces total volatyle fatty acids (VFA), acetate and methane (CH4) production higher than corn oil (P<0.05), but the efficiency of converting hexose energy to VFA (ECH) was lower (P<0.05) (76.09 vs 77.80%). Supplementation of total protected oil decreased in the protozoa population, resulting in higher dry matter digestibility (DMD) and organic matter digestibility (OMD), but lower ECH yield compared to partial protected oil supplementation (P<0.05), ie 76.68 vs 77.22%. The conclusions of the study are corn oil produce of ECH higher than palm oil. Partial protection produce better feed fermentability and increasing energy efficiency in the form of decreasing A/P ratio and methane production.

scholarly journals Effects of ractopamine hydrochloride on nutrient digestibility and nitrogen excretion of finishing beef cattle

2021 ◽  
Author(s):  
B N Harsh ◽  
B J Klatt ◽  
M J Volk ◽  
A R Green-Miller ◽  
J C McCann
Keyword(s):  
Rumen Fluid ◽  
Block Design ◽  
Dietary Treatment ◽  
Feedlot Cattle ◽  
Nutrient Digestibility ◽  
Nitrogen Excretion ◽  
Distillers Grains With Solubles ◽  
Ractopamine Hydrochloride ◽  
Urinary Nitrogen

Abstract The objective was to quantify the effects of the beta-adrenergic agonist (β-AA) ractopamine hydrochloride (Actogain, Zoetis, Parsippany, NJ) on nitrogen excretion and nutrient digestibility in feedlot cattle. In experiment 1, twelve Simmental × Angus steers were blocked by bodyweight (531 ± 16 kg) and used in a randomized complete block design. Dietary treatments included: 1) a control without β-AA (CON) or 2) 400 mg/steer/d ractopamine hydrochloride (RAC) for 35 d before slaughter. Diets contained (DM basis) 55% dry rolled corn, 20% corn silage, 15% modified wet distillers grains with solubles, and 10% supplement. For each block, total collection of feed, orts, feces and urine were conducted for two 5 d sampling periods during week 2 and 4 of RAC supplementation. No interaction (P &gt; 0.21) between treatment and collection period was observed for any parameter evaluated. Dietary treatment had no effect (P = 0.51) on DMI, but RAC had decreased fecal DM output (P = 0.04) compared with CON. Thus, RAC had greater apparent total tract DM digestibility (77.2 vs. 73.5%; P &lt; 0.01), N digestibility (72.4 vs. 69.4%; P = 0.01), and NDF digestibility (65.6 vs. 60.2%; P &lt; 0.01) than CON. Although treatment did not affect nitrogen intake (P = 0.52), RAC tended to reduce total nitrogen excretion (113.3 vs. 126.7 g/d; P = 0.10) compared with CON due to a tendency for decreased fecal nitrogen output (53.9 vs. 61.3 g/d; P = 0.10). However, dietary treatment had no effect (P = 0.53) on urinary nitrogen output or percentage of urinary nitrogen excreted as urea (P = 0.28). Experiment 2 was an in vitro experiment conducted to validate the effects of RAC on nutrient digestibility using Simmental × Angus heifers (451 ± 50 kg). Rumen fluid was collected individually by stomach tube from CON- (n = 9) and RAC-fed (n = 10) heifers to inoculate bottles containing a CON or RAC-containing substrate in a split-plot design. No interaction between rumen fluid source and in vitro substrate was observed. Greater IVDMD (P = 0.01) was observed in rumen fluid from RAC-fed heifers compared with rumen fluid from CON-fed heifers. Inclusion of RAC in the in vitro substrate increased IVDMD (P &lt; 0.01). Overall, feeding RAC increased microbial digestion of the dry-rolled corn-based finishing diet to increase total tract dry mater digestion by 5% and reduce nitrogen excretion by 10.6% in the 35 d period prior to slaughter.

scholarly journals Nutritional value of sorghum silages

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Significance Level ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
Nutritional Characteristics ◽  
High Participation ◽  
Sorghum Genotypes

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 

scholarly journals Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age

2020 ◽  
pp. 368-376
Author(s):  
La Malesi ◽  
Takdir Saili ◽  
Ali Bain ◽  
Tresjia C Rakian
Keyword(s):  
Block Design ◽  
Animal Husbandry ◽  
Study Data ◽  
Nutrient Digestibility ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Ph Level

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (<em>p</em><0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (<em>p</em>>0.05), whereas among the defoliation age treatments were significantly different (<em>p</em><0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.

scholarly journals In-vitro nutrient degradability of complete feed containing myristic acid and tannins addition

2022 ◽  
Vol 335 ◽  
pp. 00047
Author(s):  
Poespitasari Hazanah Ndaru ◽  
Siti Chuzaemi ◽  
Muhimmatu Mufidah
Keyword(s):  
Myristic Acid ◽  
Condensed Tannin ◽  
Block Design ◽  
Nutrient Content ◽  
In Vitro Digestibility ◽  
Corn Straw ◽  
Leaf Meal ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility

scholarly journals IN VITRO EVALUATION OF CONCENTRATE FEED CONTAINING Indigofera zollingeriana IN GOAT

2016 ◽  
Vol 41 (4) ◽  
pp. 196 ◽  
Author(s):  
S. Suharlina ◽  
D. A. Astuti ◽  
N. Nahrowi ◽  
A. Jayanegara ◽  
L. Abdullah
Keyword(s):  
Block Design ◽  
Maximum Level ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Concentration Data ◽  
Dry Matter Digestibility ◽  
In Vitro Technique ◽  
Significant Difference ◽  
Indigofera Zollingeriana

This study was conducted to evaluate the fermentability and nutrient digestibility of concentrate feed containing Indigofera zollingeriana using in vitro technique. Randomized complete block design was used on 3 types of concentrate feed and 4 groups (replicates) of goat's rumen liquid. The concentrate feeds were R1, R2 and R3 containing I. zollingeriana at 0 (control), 20 and 40%, respectively. Variables observed were total gas production, in vitro dry matter digestibility (IVDMD), organic matter (IVDOM), and crude protein (IVDCP), volatile fatty acid (VFA) profiles and NH3 concentration. Data were analyzed using analysis of variance (ANOVA). Results showed that there was no significant effect of R3 on IVDMD, IVDOM and IVDCP as compared to R1 and R2. However, there was significant difference (P<0.05) of the parameters between R1 and R2 (P<0.05). There was no significant effect (P<0.05) of different Indigofera levels on total gas production. The partial VFA of each concentrate feeds was not significantly different, but the percentage of acetic acids of concentrates containing I. zollingeriana were higher than those of control (P<0.05). The concentration of rumen NH3 of R2 was lower than R1 and R3 (P<0.05) even though the IVCPD of R2 was higher than R1. It could be concluded that the inclusion of I. zollingeriana as concentrate feed ingredient at 20% maximum level .

scholarly journals Adaptation of in vitro methodologies to estimate the intestinal digestion of lipids in ruminants

2021 ◽  
Vol 5 (3) ◽  
Author(s):  
James R Vinyard ◽  
Efstathios Sarmikasoglou ◽  
Sarah L Bennett ◽  
Jose A Arce-Cordero ◽  
Glen Aines ◽  
...  
Keyword(s):  
Corn Oil ◽  
Block Design ◽  
Glycerol Concentration ◽  
Free Glycerol ◽  
Lipid Digestion ◽  
Buffer Solution ◽  
Lipid Source ◽  
Randomized Block Design ◽  
Intestinal Digestion

Abstract The objective of this study was to adapt existing in vitro methodologies to determine the extent of intestinal digestion of corn oil (CO), canola oil (CA), and beef tallow (BT) via manipulation of incubation length and concentrations of lipase, bile, and calcium within a buffer solution. Unless otherwise stated, 0.5 g of each lipid source were incubated separately and in triplicate, with triplicate batch culture runs for each treatment in 40 mL of 0.5 M KH2PO4 (pH = 7.6) for 24 h with pancreatin (8 g/L), bovine bile (2.5 g/L), and CaCl2 (10 mM). Individually, concentrations of pancreatin, bile, and CaCl2, as well as incubation length were tested. To examine the use of this assay to estimate in vitro total tract digestion, a KH2PO4 solution with concentrated amounts to reach the same final concentrations of pancreatin, bile, and Ca were used as the third step in a three-step total tract digestibility procedure. Free glycerol and free fatty acid (FFA) concentrations were measured using colorimetric assays as indicators of digestion. Data wereanalyzed as a completely randomized block design (block = run), using the Glimmix procedure of SAS. For each lipid source, free glycerol increased with increasing pancreatin; however, FFA was lowest at 0 g/L pancreatin but was similar at 6, 8, and 10 g/L. Both glycerol and FFA were greater for 2.5 and 5 g/L of bile than for 0 g/L for each lipid source. Calcium concentration did not affect glycerol or FFA for either CO or CA; however, glycerol and FFA for BT were greater when calcium was included at 5 and 10 mM than at 0 mM. For all fat sources, free glycerol and FFA increased after 1 h until 12 h, but did not increase from 12 to 24 h. When a concentrated mixture was used following fermentation and acidification steps, digestibility using FFA concentration increased as compared to just adding buffer; however, free glycerol concentration was indeterminable. Thus, free glycerol and FFA can be used as indicators of lipid digestion when a lipid source is incubated for at least 12 h in a buffer solution containing 8 g/L pancreatin, 2.5 g/L bile, and 5 mM Ca when only estimating in vitro intestinal digestion; however, when utilizing this assay in a three-step in vitro total tract digestibility procedure, only FFA can be used.

scholarly journals Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Block Design ◽  
Gas Production ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
In Vitro Gas Production

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

scholarly journals KERCERNAAN IN VITRO BAHAN KERING DAN BAHAN ORGANIK SERTA PROTEIN RANSUM BERBASIS PAKAN FERMENTASI

2014 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
Aang Baitul Mizan ◽  
Andi Murlina Tasse ◽  
Deki Zulkarnain
Keyword(s):  
Organic Matter ◽  
Experimental Design ◽  
Rice Bran ◽  
Dry Matter ◽  
Block Design ◽  
Animal Nutrition ◽  
Animal Science ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

This study has conducted four weeks in Laboratory of Animal Nutrition Department of Animal Science, Faculty of Animal Science, Haluoleo University, Kendari. The treatments R0 = rations based feed’s non fermented, R1 = rations based 40% rice bran fermented (RBF), R2 = rations based 45% RBF, R3 = rations based 50% RBF, R4 = rations based 55% RBF, R5 = rations based 60% RBF. The experimental design used a randomized block design (RBD) with 5 treatments and 4 groups. The results showed that dry matter digestibility (DMD) of R1, R2, R5 higher than R0 (94,17%, 90,98%, 91,04% vs 88.60%). In contrast, DMD of R3 and R4 lower than R0 (80,90% and 80,89% vs 88,60%). Organic matter digestibility (OMD) of R0 lower than R1, R2, R3, R4 and R5 ( 62,58% vs 90,32%, 90,55%, 90,78%, 91,15% and 90,41%). In contrast, OMD of R4 higher than R1, R2, R3, R5 and R0 (91,41% vs 90,32%, 90,55%, 90,78%, 90,41% and 62,58%). Ammonia (NH3) concentrations of R1, R2, R3, R4 and R5 higher than R0 (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM and 7,42 mM vs 3,20 mM). The conclusion of this study was FBR based 40%, 45%, 50%, 55% and 60% RBF feasible to ruminant’s feed.Keywords : Feed’s Fermented, Digestibility, Dry Matter, Organic Matter, Ammonia.ABSTRAKPenelitian ini bertujuan mengevaluasi kelayakan penggunaan ransum berbasis pakan fermentasi untuk ternak ruminansia berdasarkan kecernaan bahan kering dan bahan orgaik serta protein secara In Vitro. Penelitian ini dilaksanakan selama empat minggu di Laboratorium Ilmu Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Haluoleo, Kendari. Perlakuan yang dicobakan adalah R0= ransum berbasis pakan non fermentasi (kontrol), R1= ransum berbasis dedak padi fermentasi 40%, R2= ransum berbasis dedak padi fermentasi 45%, R3= ransum berbasis dedak padi fermentasi 50%, R4= ransum berbasis dedak padi fermentasi 55%, R5= ransum berbasis dedak padi fermentasi 60%. Rancangan percobaan yang digunakan rancangan acak kelompok (RAK) dengan 5 perlakuan dan 4 kelompok. Hasil analisis ragam menunjukkan kecernaan bahan kering ransum berbasi dedak padi fermentasi 40%, 45%, dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (94,17%; 90,98% dan 91,04% dibanding dengan 88,60%). Sebaliknya, ransum berbasis dedak padi fermentasi 50% dan 55% lebih rendah dibanding dengan ransum berbasis pakan non fermentasi (80,90% dan 80,89% dibanding dengan 88,60%). Kecernaan bahan organik ransum berbasis pakan non fermentasi lebih rendah dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% (62,58 % dibanding dengan 90,32 %, 90,55 %, 90,78 %, 91,15 % dan 90,41 % ). Sebaliknya kecernaan bahan organik ransum berbasis dedak padi fermentasi 55% lebih tinggi dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 60% dan kontrol (91,41% dibanding dengan 90,32%, 90,55%, 90,78%, 90,41% dan 62,58%). Konsentrasi amonia ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM dan 7,42 mM dibanding dengan 3,20 mM). Kesimpulan bahwa ransum berbasis dedak padi fermentasi 40% hingga 60% layak digunakan untuk pakan ternak ruminansia.Kata kunci: Pakan Fermentasi, Kecernaan Bahan Kering, Kecernaan Bahan Organik, Amonia.

scholarly journals The potential of frozen rumen fluid for ruminant feed evaluation using in vitro gas production technique

2022 ◽  
Vol 335 ◽  
pp. 00053
Author(s):  
Melyndra Sonya Pramita ◽  
Hendrawan Soetanto
Keyword(s):  
Storage Time ◽  
Rumen Fluid ◽  
Block Design ◽  
Gas Production ◽  
Indigenous Cattle ◽  
Inoculum Source ◽  
Randomized Block Design ◽  
Feeding Regimes ◽  
Ruminant Feed

The aim of this study was to determine the potential of frozen rumen fluid (RF) for ruminant feed evaluation using in vitro gas production (IVGP) technique. This study used a factorial randomized block design with a 3 × 3 × 3 pattern, consisting three feeding regimes varying in forage to concentrate proportion, namely 20%:80% (feed A), 40%:60% (feed B), and 60%:40% (feed C), respectively; three storage time (2, 11, and 19 days); and RF from 3 breeds of indigenous cattle, namely Bali, crossbred Ongole and Madura cattle as groups. The results showed that the effects of feeding regimes, inoculum source, or storage time on total gas production, potential of gas production, rate of gas production, dry matter and organic matter degradability. Based on the results, it can be concluded that frozen RF may be used as inoculum source for feed evaluation using the IVGP technique and hence it supports the previous findings reported in the literature. Considering that the effects of different feeds, inoculum sources, and storage times gave the specific values, it is advisable that to use RF from slaughtered animals should be taken from the same breed to allow accurate comparison for feed evaluation using IVGP technique.

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