scholarly journals In-vitro nutrient degradability of complete feed containing myristic acid and tannins addition

2022 ◽  
Vol 335 ◽  
pp. 00047
Author(s):  
Poespitasari Hazanah Ndaru ◽  
Siti Chuzaemi ◽  
Muhimmatu Mufidah

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility

2022 ◽  
Vol 335 ◽  
pp. 00028
Author(s):  
Siti Chuzaemi ◽  
Iftitah Nuzulia Qur’any

The study aimed to determine the effect of adding tannin and myristic acid in complete feed on nutrient content and the Profile of volatile fatty acids (VFA) in vitro. The materials used for this research were corn straw, condensed tannins, myristic acid, coffee waste, rice bran, tapioca byproduct, soybean meal, copra meal, and palm kernel meal. The method was used in this experiment was laboratory experiment with four treatments and three replications. The treatment consisted of T1 complete feed (40% corn straw + 60% concentrate (be based DM)), T2 (complete feed + condensed tannins 3%/kg DM and myristic acid 2%/kg DM), T3 (complete feed + condensed tannins 3%/kg DM and myristic acid 3%/kg DM), and T4 (complete feed + condensed tannins 3%/kg DM and myristic acid 4%/kg DM). Every treatment was repeated three times and analysed statistically using Randomized Block Design (RBD). The adding of 4% myristic acid to the complete feed (T4) gives the best results, it increased nutrition and propionic acid, but decreased acetic acid, butyric acid, total VFA and C2/C3 ratio.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 404-404
Author(s):  
Leeroy A Lente ◽  
Roderick A Gonzalez Murray ◽  
Shawn L Archibeque

Abstract The aim of this study was to assess the effects of Zeolite (ZE, clinoptilolite) inclusion on in vitro rumen fermentation. A modified procedure from Tilley and Terry (1963) was used to determine alfalfa in vitro dry matter digestibility (IVDMD) in the presence or absence of ZE. Test tubes (n = 96) were placed in a 39 °C bath and were blocked based on hours 0, 1, 2, 4, 6, 8, 12, 24, and 48. Substrate consisted of dried alfalfa grinded through a 1mm screen using a Wily Mill. Treatments consisted of, Control (no ZE inclusion), and 0.05g ZE, for each 1 gram of alfalfa. After incubation, IVDMD was calculated 24 hours after drying period. Data was analyzed using a randomized block design. The IVDMD was found to be similar across all treatments (P > 0.8464). The IVDMD was significantly different when incubated for the varying lengths of time (P < 0.001). There was an IVDMD of 20.18 ± 2.89 % for the control and 18.07 ± 5.49 % for the ZE at the zero hour. The IVDMD for Control and ZE for 48 hours were 54.30 ± 1.58 % and 53. 48 ± 1.04 % respectively. To predict the IVDMD of the effects of ZE treatment on digestibility over time, the corresponding regression f(x) = 20.60 + 0.421X1 + 0.730X2 with R2 = 0.8464. In conclusion these data demonstrate that inclusion of ZE, does not influence the in vitro digestibility of alfalfa. These findings are important as previous data would indicate that inclusion of ZE may reduce cost of gain and/or influence liver abscess rates, it was uncertain if that would come at the cost of reduced digestibility of feed. These data indicate that there is likely very little to no impact on feed digestibility when ZE is included in the ration.


2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 


2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.


1978 ◽  
Vol 58 (4) ◽  
pp. 775-781 ◽  
Author(s):  
R. R. MARQUARDT ◽  
J. A. McKIRDY ◽  
A. T. WARD

A series of experiments has been carried out with tannin-free and tannin-containing cultivars of fababeans. The in vitro dry matter digestibility of the cotyledon portion of the two groups of fababeans was the same (88.2%) whereas the in vitro digestibility of the testa portion of the tannin-free cultivars was much higher (27.6%) than that of the tannin-containing cultivars (9.2%). The concentrations of certain cell wall constituents in the testae of tannin-free and tannin-containing cultivars were also different (P < 0.01); the respective average values for the two groups were: condensed tannins, 0.2 and 4.9%; lignin, 4.2 and 6.2%; acid detergent fiber, 72 and 66%; and cellulose, 67 and 60%. There was a negative correlation between the in vitro dry matter digestibility and the amount per unit weight of whole beans of either condensed tannin (r = −0.86, P < 0.01) or lignin (r = −0.89, P < 0.01). When expressed on a similar basis there was no correlation between levels of cellulose or acid detergent fiber and in vitro dry matter digestibility.


2018 ◽  
Vol 28 (3) ◽  
pp. 213
Author(s):  
Eko Marhaeniyanto ◽  
Sri Susanti

The aim of this research was to study the in vitro ruminal fermentability of supplementation of <em>Gliricidia sepium, Moringa oleifera</em>, Lamk (<em>MOL</em>), <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed to be tested on sheep.<strong> </strong>This research with the experimental methods was arranged in Randomized Block Design (RBD) with 6 treatments and 3 groups. The treatment feed tested consisted of concentrate without leaves and concentrate with leaf meal supplementation. Crude protein content (CP) concentrates were prepared 16%, 18% and 20%. Supplementation uses a mixture of <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed (1: 1: 1: 1) as much as 10%, 20% and 30%. The measured variables were  degradation of dry matter (DDM) and degradation of organic matter (DOM), gas production rate, microbial biomass and NH<sub>3</sub> concentration. The use of mixed leaf meal in concentrate feed resulted in in vitro DDM as well as concentrate feed without leaf meal supplementation. Supplementation of leaf meal in concentrate feed as much as 30% with CP 20% resulted in a decrease in the value of fermentability. The supplementation of leaf meal in concentrate feed as much as 20% in concentrate feed with CP 18% produced the best fermentability value. Suggested for in-vivo trials on sheep using 18% protein concentrate feed by utilizing <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed as cheap protein sources.


Author(s):  
La Malesi ◽  
Takdir Saili ◽  
Ali Bain ◽  
Tresjia C Rakian

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (<em>p</em><0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (<em>p</em>>0.05), whereas among the defoliation age treatments were significantly different (<em>p</em><0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.


2011 ◽  
Vol 40 (4) ◽  
pp. 708-714 ◽  
Author(s):  
H. Caetano ◽  
M.D.S. Oliveira ◽  
J.E. Freitas Júnior ◽  
A.C. Rêgo ◽  
M.V. Carvalho ◽  
...  

The objective of this study was to evaluate the nutritional traits and in vitro digestibility of silages from different corn cultivars harvested at two cutting heights. It was evaluated 11 cultivars (Dina 766, Dina 657, Dina 1000, P 3021, P 3041, C 805, C 333, AG 5011, FO 01, Dina co 9621 and BR 205) harvest 5 cm above ground (low) and 5 cm below the intersection of the first ear (high). It was used a random block design (three blocks), arranged in a 11 × 2 factorial scheme. Silages from plants harvested at high cutting height presented average content of dry matter significantly superior to silages from plants harvested at low height. Cultivars FO 01, AG 5011, Dina co 9621 and Dina 766 presented greater content of crude protein than cultivars C 805, P 3041 and P 3021, which presented the lowest contents of this nutrient. The raise in the cut height increased in vitro dry matter true digestibility coefficients and in vitro dry matter digestibility of silage evaluated. The increase in cut height improved nutritive value of silages by decreasing concentrations of fibrous fractions and increasing in vitro dry matter digestibility.


2020 ◽  
Vol 21 (1) ◽  
pp. 22
Author(s):  
Anis Muktiani ◽  
Nurul Arifah ◽  
Widiyanto Widiyanto

This research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments.


2019 ◽  
Vol 43 (3) ◽  
Author(s):  
Laily Ismatul Faizah ◽  
Widiyanto Widiyanto ◽  
Anis Muktiani

This study was examines the effect of oil (oil palm and corn) combination with the level of protection (total or partial) on feed fermentability, methane production and energy efficiency with in vitro techniques. The experiment was designed using a factorial pattern 2x2, factor A = type of oil (corn and palm) and factor B = oil protection level (total and partial), each treatment combination was repeated 4 times. Data was processed by analyzing various factorial patterns in a randomized block design and if there was an influence between treatments performed by the Dunca test. The results showed that there was no interaction effect (P>0.05) between the types of oil with the level of protection in all parameters, except NH3. Supplementation of palm oil produces total volatyle fatty acids (VFA), acetate and methane (CH4) production higher than corn oil (P<0.05), but the efficiency of converting hexose energy to VFA (ECH) was lower (P<0.05) (76.09 vs 77.80%). Supplementation of total protected oil decreased in the protozoa population, resulting in higher dry matter digestibility (DMD) and organic matter digestibility (OMD), but lower ECH yield compared to partial protected oil supplementation (P<0.05), ie 76.68 vs 77.22%. The conclusions of the study are corn oil produce of ECH higher than palm oil. Partial protection produce better feed fermentability and increasing energy efficiency in the form of decreasing A/P ratio and methane production.


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