scholarly journals Analisis Komposisi Biodiesel Hasil Konversi Minyak Biji Carica (Carica pubescens) Menggunakan Enzim Lipase Bekatul

10.20885/vol1 ◽  
2018 ◽  
Vol 1 (01) ◽  
Author(s):  
Reni Banowati Istiningrum ◽  
Hastin Nurrokhmah ◽  
Astri Sri Wahyuni
Keyword(s):  
Carica Pubescens

Peningkatan konsumsi bahan bakar berbasis fosil mendorong berbagai penelitian untuk memproduksi bahan bakar yang bersifat terbarukan salah satunya biodiesel. Biodiesel adalah bahan bakar yang bersumber dari minyak nabati serta ramah lingkungan. Minyak biji carica berpotensi sebagai sumber minyak nabati untuk produksi biodiesel karena merupakan limbah home industry pengolahan buah carica dari Kabupaten Wonosobo dan merupakan nonedible oil. Penelitian ini bertujuan untuk memproduksi biodesel dari minyak biji carica secara enzimatis menggunakan enzim lipase yang diekstrak dari bekatul. Hasil penelitian ini menunjukkan sebesar 50,36 % minyak dapat diekstrak dari biji carica kering dengan komposisi terbesarnya adalah asam oleat. Enzim lipase dapat diekstrak dari bekatul menggunakan buffer pH 5,5 dengan nilai aktivitas sebesar 14,72 U/mL. Ekstrak enzim lipase ini juga menunjukkan sifat stabil selama penyimpanan 15 hari. Minyak biji carica berhasil dikonversi menjadi biodiesel dengan rasio molar minyak : metanol optimum adalah 1 : 6 dan rendemen yang dihasilkan sebesar 65,86% dengan kadar metil ester sebesar 98,7% relatif.

scholarly journals IDENTIFIKASI SENYAWA ANTIDIABETES SECARA in Silico PADA Carica pubescens Lenne & K. Koch

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 135
Author(s):  
Ainun Nikmati Laily ◽  
Ahmad Nuruddin Khoiri
Keyword(s):  
In Silico ◽  
Raw Materials ◽  
Iridoid Glycosides ◽  
Cysteine Proteases ◽  
Central Java ◽  
Chemical Content ◽  
Plateau Central ◽  
Sugar Levels ◽  
Carica Pubescens

<em>Carica pubescens Lenne &amp; K. Koch is one of the typical highland crops in Indonesia. This plant can be found in the region of Cangar and Bromo in East Java, as well as the Dieng Plateau, Central Java Wonosobo-Banjarnegara. Morphological, chemical content, and analysis of protein banding pattern of the Carica pubescens has been done, but more about the use of active compounds for pharmaceutical raw materials, in Diabetes Mellitus type 2 have not been studied. One therapeutic approach to lowering blood sugar levels posprandial is to slow down or inhibit the absorption of glucose through inhibition of the carbohydrates hidrolitic enzyme such as α-amylase and α-glucosidase. This study aims to identify antidiabetic compounds in silico. Antidiabetic compounds were analyzed by methods of docking. The results showed that papain and cysteine protease in C. pubescens have better binding affinity to α-amylase and α-glucosidase compared to iridoid glycosides. Papain and cysteine proteases isolated from C. pubescens are potential to be developed as an antidiabetic.</em>

scholarly journals ANALISIS KANDUNGAN SAPONIN PADA DAUN DAN TANGKAI DAUN Carica pubescens Lenne & K. Koch

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 143 ◽  
Author(s):  
Eko Budi Minarno
Keyword(s):  
Raw Materials ◽  
Herbal Medicines ◽  
The Body ◽  
Raw Material ◽  
Ring Test ◽  
Tropical Species ◽  
Color Test ◽  
Natural Medicine ◽  
Chemical Content ◽  
Carica Pubescens

<p><em>Carica pubescens Lenne &amp; K. Koch is a tropical species that adapt to the plateau environment and low temperatures. In East Java, the plant is found in Cangar and Bromo region. Morphological, chemical content, and analysis of protein banding pattern on C. pubescens has been done, but more on the analysis of active compounds for pharmaceutical raw materials and its accumulation in the body of the plant has not been widely studied. Saponins on C. pubescens potential as a raw material of natural medicine in the treatment of Diabetes Mellitus (DM). This study aims to determine the content of saponin in leaf and petiole of C. pubescens in terms of absorbance values. Saponins were analyzed by qualitative form the foam test, color test, Thin Layer Chromatography (TLC) analytical and preparative. Quantitative test in the form of UV-Vis spectrophotometry results preparative TLC. This research was done at the Laboratory of Department of Biology and Chemistry UIN Maulana Malik Ibrahim Malang. The results showed that the leaf and petiole of C. pubescens positive for the saponins with the formation of stable foam for 60 seconds at 1.5 cm - 1.7 cm. The third positive samples containing saponins triterpene the ring test produces a brownish color. Isolation saponin by TLC shows the best ratio of eluent chloroform: methanol: water (14: 6: 1) compounds can be separated perfectly. Saponin absorbance values obtained three samples as follows: petiole samples from the region Cangar amounted to 0.852, leaf samples from the region Cangar amounted to 0.686, and leaf samples from Bromo region amounted to 0,629. The highest saponins found in organs petiole. Thus the petiole of C. pubescens has the potential to be used as a source of triterpene saponins which can be developed into a commercial herbal medicines.</em></p>

scholarly journals PENGARUH EKSTRAK BUAH Carica pubescens Lenne & K. Koch YANG TUMBUH DI BEBERAPA TEMPAT DI INDONESIA TERHADAP PENYEMBUHAN LUKA MUKOSA RONGGA MULUT

el–Hayah ◽  
2015 ◽  
Vol 5 (3) ◽  
pp. 123
Author(s):  
Risma Aprinda Kristanti
Keyword(s):  
Wound Healing ◽  
Adult Male ◽  
Albino Rats ◽  
Gingival Mucosa ◽  
K2 Group ◽  
Carica Pubescens

<em>The aim of this study is to examine the effect of Carica  pubescens</em> Lenne &amp; K. Koch <em>extract to the epihtelialization on the wound healing of gingival mucosa and to examine the effect of C. pubescens extract from different region (Dieng and Cangar) to the epithelialization process.Twenty eight adult male albino rats were devided into four group, we created cutlet along 1 cm on the mandibular gingival mucosa below insisiv teeth. K1 group were treated with aquadest while K2 group were treated with C. pubescens extract from Dieng, K3 group were treated with C. pubescens extract from Cangar, and K4 group were treated with policresulen preparations. At the end of fifth day, the rats were sacrificed and the mandibular gingival mucosa were collected for histologist preparations. The result of this study shows that K3 group has the thickest gingival epithel layers while there is no significant different among four group for the thikness gingival epithel layers</em>

scholarly journals SKRINING FITOKIMIA DAN KANDUNGAN TOTAL FLAVANOID PADA BUAH Carica pubescens Lenne & K. Koch DI KAWASAN BROMO, CANGAR, DAN DATARAN TINGGI DIENG

el–Hayah ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 73 ◽  
Author(s):  
Eko Budi Minarno
Keyword(s):  
Positive Sample ◽  
Screening Tests ◽  
Total Flavonoids ◽  
Test Results ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Third ◽  
Fruit Samples ◽  
The Tropics ◽  
Carica Pubescens

<p class="Default"><em>Carica  pubescens Lenne &amp; K. Koch is one of the species in the tropics, which adapt to the plateau environment and low temperatures. In Indonesia, this plant is found at Cangar, Bromo, and Dieng Plateau. This study aims to determine the results of phytochemical screening and total flavonoids in fruit samples were taken from the third place. Qualitative and quantitative tests carried out in the Laboratory of Department of Biology and Chemistry, </em><em>Faculty of </em><em>Science and Technology</em><em>, </em><em>Maulana Malik Ibrahim State Islamic University, Malang</em><em>. Analysis of total flavonoids using a spectrophotometer at λ = 510 nm. The results of qualitative phytochemical screening tests on samples of C. pubescens fruits at Cangar, Bromo, and Dieng Plateau shows that the positive sample contains flavonoids, polyphenols, tannins, and triterpenoids. Quantitative test results show that the C. pubescens at Cangar contains  total flavonoids quercetin equivalent with value 800 mg / L, Bromo with value 816.65 mg / L, and Dieng Plateau with value 633.35 mg / L, respectively.</em></p>

scholarly journals Evaluación de efecto antibacteriano in vitro de la harina de látex de Carica pubenscens en Escherichia coli y Salmonella typhimurium

2021 ◽  
Vol 4 (1) ◽  
pp. 91
Author(s):  
Darleny Tenorio Cruz ◽  
Reiner Pedro Gabriel Reátegui Inga ◽  
Manuel Emilio Reátegui Inga
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Carica Pubescens

<p>El objetivo de la presente investigación fue evaluar el efecto antibacteriano <em>in vitro </em>del látex de la papaya silvestre <em>(Carica pubescens) </em>en <em>Escherichia coli </em>y <em>Salmonella typhimurium</em>, para esto se colectaron muestras de heces de cuyes con presencia de diarreas de la granja PROALCUY. Los ensayos fueron realizados en el Laboratorio de Enfermedades Infecciosas y Parasitarias de Animales Domésticos de la Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM), con tres tratamientos y un grupo testigo, cada una con 4 repeticiones, se usaron concentraciones de 0, 20, 40 y 60% del látex de papaya silvestre. Los datos obtenidos fueron procesados por análisis de varianza (Diseño Completamente al Azar) y comparación de medidas Tukey con un nivel de significancia del 0,05. El efecto antibacteriano frente a <em>Escherichia coli </em>presento un tamaño de halo de 6 mm en todos los tratamientos mientras que en la <em>Salmonella typhimurium </em>6, 6, 10,75 y 11,25 mm para las concentraciones 0, 20, 40 y 60% de la harina de látex de papaya silvestre, respectivamente, evidenciando que el látex de papaya silvestre no tiene efecto antibacteriano frente a <em>Salmonella typhimurium </em>y <em>Escherichia coli</em>.</p>

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