scholarly journals Pengaruh pohon induk dan bahan stek terhadap pertumbuhan stek cabang Timoho ( Kleinhovia hospita L)

1996 ◽  
Vol 13 (2) ◽  
pp. 123-130
Author(s):  
Lukman Hakim ◽  
◽  
Yuliah Yuliah ◽  
Hamdan Adma Adinugraha
Keyword(s):  
Research Method ◽  
Tree Improvement ◽  
Shoot Length ◽  
Raw Material ◽  
Cultural Value ◽  
Stem Cuttings ◽  
Factorial Experimental Design ◽  
Forest Biotechnology ◽  
Number Of Leaves ◽  
Number Of Shoots

Timoho (Kleinhovia hospita L) is a rare plant that has high economic and cultural value for the Javanese people, especially in Yogyakarta. Timoho wood has a beautiful pattern called pelet and is used as a raw material for keris sheath. In Yogyakarta, timoho trees are difficult to find, currently, so that plant propagation in a generative way also has difficulty due to scarcity of seeds. Therefore, it is necessary to conduct timoho propagation research through vegetative methods. The purpose of this study was to determine the origin of stem cuttings to the success of timoho stem cuttings. Stem cuttings come from three timoho trees in the Arboretum of the Center for Forest Biotechnology and Tree Improvement Yogyakarta. The research method used a factorial experimental design using 3 mother trees and 3 stem parts (base, middle and end). The parameters observed were percentage of shoots, number of shoots, length of shoots, and number of leaves. The results showed the percentage of live cuttings varied from 10.9-60.0%, number of shoots 1-7 pieces, number of leaves 3-26 strands and length of shoots 2.00-17.7 cm. The mother tree has a significant effect on the percentage of sprouts and shoot length, while the cuttings material has a significant effect on the length of shoots at the cuttings measurements of the age of 2 months since planted in polybags.

scholarly journals Pengaruh pohon induk dan bahan stek terhadap pertumbuhan stek cabang Timoho ( Kleinhovia hospita L)

1996 ◽  
Vol 13 (2) ◽  
pp. 123-130
Author(s):  
Lukman Hakim ◽  
◽  
Yuliah Yuliah ◽  
Hamdan Adma Adinugraha
Keyword(s):  
Research Method ◽  
Tree Improvement ◽  
Shoot Length ◽  
Raw Material ◽  
Cultural Value ◽  
Stem Cuttings ◽  
Factorial Experimental Design ◽  
Forest Biotechnology ◽  
Number Of Leaves ◽  
Number Of Shoots

Timoho (Kleinhovia hospita L) is a rare plant that has high economic and cultural value for the Javanese people, especially in Yogyakarta. Timoho wood has a beautiful pattern called pelet and is used as a raw material for keris sheath. In Yogyakarta, timoho trees are difficult to find, currently, so that plant propagation in a generative way also has difficulty due to scarcity of seeds. Therefore, it is necessary to conduct timoho propagation research through vegetative methods. The purpose of this study was to determine the origin of stem cuttings to the success of timoho stem cuttings. Stem cuttings come from three timoho trees in the Arboretum of the Center for Forest Biotechnology and Tree Improvement Yogyakarta. The research method used a factorial experimental design using 3 mother trees and 3 stem parts (base, middle and end). The parameters observed were percentage of shoots, number of shoots, length of shoots, and number of leaves. The results showed the percentage of live cuttings varied from 10.9-60.0%, number of shoots 1-7 pieces, number of leaves 3-26 strands and length of shoots 2.00-17.7 cm. The mother tree has a significant effect on the percentage of sprouts and shoot length, while the cuttings material has a significant effect on the length of shoots at the cuttings measurements of the age of 2 months since planted in polybags.

scholarly journals PERTUMBUHAN STEK HORIZONTAL BATANG JARAK PAGAR (Jatropha curcas L.) YANG DIINTRODUKSI DENGAN PSEUDOMONAD FLUORESEN

2018 ◽  
Vol 19 (1) ◽  
pp. 68-75
Author(s):  
Linda Advinda
Keyword(s):  
Jatropha Curcas ◽  
Indole Acetic Acid ◽  
Shoot Length ◽  
Raw Material ◽  
Fluorescent Pseudomonad ◽  
Stem Cuttings ◽  
Jatropha Curcas L ◽  
Randomized Design ◽  
Shoot Number ◽  
Number Of Shoots

Jatropha curcas L. is one of the plants that can be developed as raw material of biodiesel. One of the efforts to increase the production of J. curcas is the propagation of stem cuttings horizontally. Efforts to stimulate root growth can be accomplished by adding Plant Growth Regulators (PGRs). PGRs can be obtained naturally or synthetically. Indole acetic acid (IAA) is one of PGRs, which is not only produced by plants but can also be produced by rhizobacteria. One such rhizobacteria is fluorescent pseudomonad. The objectives of the study were to know the response of growth of horizontal stem cuttings after being introduced with fluorescent pseudomonad. This research is an experimental research.  Complete Randomized Design with 6 treatments and 4 replications. The treatments were soaking of J. curcas stem cuttings with fluorescent pseudomonad isolate suspension PfPj3 suspension concentration of 0% (without suspension), 5%, 10%, 15%, 20%, and 25%. Observation of the research is the beginning of the shoots, the number of shoots, and the length of the J. curcas stem shoot. The data obtained were analyzed using Anova and DNMRT significant level of 5%. The results showed that horizontal stem cuttings of J. curcas with fluorescent pseudomonad suspension influenced early shoot, shoot number, and shoot length. A 10% fluorescent pseudomonad concentration can cause shoots to appear early, while a concentration of 20% causes the largest number of shoots.

scholarly journals Performance of mulberry cultivars (Morusspp.)in response to different stem cuttings and fertilizers

2021 ◽  
Vol 17 (4) ◽  
pp. 47-58
Author(s):  
Anelisa de Aquino Vidal Lacerda Soares ◽  
Fernanda de Paiva Badiz Furlaneto ◽  
Cláudio Hajime Funai ◽  
Eduardo Gazola
Keyword(s):  
Aqueous Solution ◽  
Factorial Design ◽  
Production Cost ◽  
Dry Matter ◽  
Shoot Length ◽  
Control Treatment ◽  
Stem Cuttings ◽  
Tukey Test ◽  
K Fertilizer ◽  
Number Of Leaves

The objective is to analyze techniques to form mulberry seedlings with different cutting sizes and forms of fertilization with N-P-K aiming a low production cost and an easy application in field. The experiment was carried out in a screened nursery, with a shading of 34%. The treatments were distributed in randomized blocks (RB) and compared in a 2x4x3 factorial design with five replications and 12 plants per plot. The factorial design consisted of two cutting lengths (10 and 20 cm) distributed in plastic tubes of 70 and 120 mL, four mulberry cultivars (IZ40, IZ10/1, Korin, and IZ3/2), and two types of fertilization: addition of 84 g of Osmocote® for each kilo of substrate, fertigation with aqueous solution containing 84 g of 14-14-14 N-P-K for each kilo of substrate, plus a control treatment (without fertilization). The commercial substrate Basaplant citrus® was used. 90 and 140 days after planting, assessments of percentage of rooting were carried out. At 140 days, shoot length (cm), number of shoots, number of leaves, root length (cm), and shoot and root green and dry matter were evaluated. The data obtained were evaluated using the Sanest software and Tukey test (P<0.05). Cuttings of 20 cm in length in tubes of 120 mL are recommended for the best formation of mulberry seedlings with the application of N-P-K fertilizer through fertigation. The cultivars showed different rooting potentials and shoot development. The best results are for Korin and IZ 10/1.

scholarly journals Pengaruh asal benih timoho (kleinhovia hospita L.) terhadap pertumbuhan bibit di tingkat persemaian

2020 ◽  
Vol 14 (1) ◽  
pp. 63-72
Author(s):  
Rafelinta Daradwinta ◽  
Ragil Pinasti ◽  
Keyword(s):  
Leaf Blade ◽  
Raw Materials ◽  
Genetic Material ◽  
Petri Dish ◽  
Hot Water ◽  
Raw Material ◽  
Stem Length ◽  
Forest Biotechnology ◽  
Seed Extraction ◽  
Number Of Leaves

Timoho (Kleinhovia hospita) wood is one of the raw materials for keris sheath that having high aesthetic and economic values. Due to exploitation of keris raw material that outmatches its planting activities, this type of tree is already hard to find in the Special Region of Yogyakarta, so this research aims to determine the generative propagation techniques carried out in the nursery of Center for Forest Biotechnology and Tree Improvement. Genetic material in the form of seeds in this study came from the parent trees grown in Bulaksumur region and Purwobinangun region, Yogyakarta. The methods that will be used are sampling, seed extraction, seed scarification in hot water for 24 hours, germination using wet tissue as media in petri dish, and planted in polybags. The result showed that viability of seeds from Bulaksumur region was 80% while that from Purwobinangun region was 68%. Observation and measurement of stem length and number of leaves every 2 weeks for 42 daysshowed that the number of timoho seed leaves from Purwobinangun hada higher number of leaves and a longer stem length, which is 19,11 ± 2,66 leaf blade and 11,54 ± 2,77 cm than the number of leaves and stem length from Bulaksumur, which is 0,59 ± 1,07 leaf blade and 7,59 ± 1,17 cm. Based on thisresearch, it was concluded that although the viability of seeds from Bulaksumur was higher, the possibility of inbreeding from it broodstock caused the growth of seeds from Bulaksumur not as good as the growth of seeds from Purwobinangun

scholarly journals Lilium longiflorum Plant Growth with a combination of Naphthylacetic Acid (NAA) and 6Benzylaminopurine (BAP) In Vitro

2019 ◽  
Vol 1 (2) ◽  
pp. 78
Author(s):  
Rosita Husnun Fauziah ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Research Method ◽  
Growth Parameter ◽  
Lilium Longiflorum ◽  
Fungal Contamination ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Naphthylacetic Acid ◽  
Number Of Shoots

This research was aim to identify the effect of Naphthylacetic Acid (NAA) and 6-Benzylaminopurin (BAP) on growth of Lilium longiflorum planlet. The research method was Completely Randomized Design Factorial 4 x 4 with 4 replication. First factor was concentration of NAA, consist of A0: 0 mg/l, A1: 0,75 mg/l, A2: 1,5 mg/l, and A3: 2,25 mg/l. Second factor was concentration of BAP, consist of : B0: 0 mg/l, B1: 0,3 mg/l, B2: 0,6 mg/l, and B3: 0,9 mg/l. The observed parameters were number of shoots, plant height, number of leaves, number of roots, and percentage of contamination. Data were process by Analysis of Variance and continued by Least Significance Different (LSD). The result showed that combination of NAA and BAP did not significantly affect the growth parameter of Lilium longiflorum. BAP treatment significantly affected the number of shoots and number of leaves. The number of roots only observed in NAA treatment; 0,3 mg/l BAP + 0,75 mg/l NAA; 0,3 mg/l BAP + 1,5 mg/l  NAA; and 0,6 mg/l BAP + 0,75 mg/l NAA. Percentage of fungal contamination was 7,5%. The conclusion of the result were the treatment 0f 0,3 mg/l BAP + 0,75 mg/l NAA was the best treatment for growth of Lilium longiflorum planlets with no contamination.

scholarly journals EFFECT OF PHYTOHORMONES AND THEIR DIVERSE CONCENTRATIONS ON REGENERATION OF ROSE (ROSA HYBRIDA L.)

2020 ◽  
Vol 23 (1) ◽  
pp. 46-51
Author(s):  
Memon Amjad Ali ◽  
Ghulam Mangrio
Keyword(s):  
Basal Medium ◽  
Rosa Hybrida ◽  
Shoot Length ◽  
Root Induction ◽  
Root Initiation ◽  
Planting Material ◽  
Number Of Leaves ◽  
Analysis Of Variation ◽  
Number Of Shoots

Roses most important regularly used for ornamental, medicinal and aromatic rationale in the world. The relevance of plant tissue culture technology to produce planting material of rose in masses depends on the availability of an effective regeneration protocol. The present experiment was done to scrutinize for appropriate basal medium of Murashige and Skoog (1962), phytohormones with their diverse concentrations influence for establish In vitro shoot and root induction of rose (Rosa hybrida L.). The statically analysis of variation explain that least days to initiation, number of shoots, length of shoot cm, number of leaves, days taken in root initiation and number of roots were significant @ 5% possibility. Increase evidence viewing that experimental conclusion exhibit that minimum days to initiation, utmost number of shoots bottle-1, shoot length bottle-1 and number of leaves bottle-1 be record within the concentration of MS + NAA 0.5 mgL-1 + BAP 2 mgL-1. Hence forward minimum days taken in root initiation, highest roots number recorded at 1/2MS + NAA 1.0mg/l + IBA 1.0 mg/l respectively. In vitro healthy and complete plantlets successfully were shifted in to different pot mixtures, supreme survival % recorded at Soil+sand+FYM (1:1:1).

scholarly journals The Effect of Mycorrhizal Inoculum and Phosphorus Treatment on Growth and Flowering of Ajania (Ajania pacifica (Nakai) Bremer et Humphries) Plant

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 178
Author(s):  
Matej Vosnjak ◽  
Matevz Likar ◽  
Gregor Osterc
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Flowering Time ◽  
Endophytic Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Growing Season ◽  
Shoot Length ◽  
Available Phosphorus ◽  
Growing Seasons ◽  
Number Of Shoots

The influence of mycorrhizal inoculum in combination with different phosphorus treatments on growth and flowering parameters of Ajania (Ajania pacifica (Nakai) Bremer et Humphries) plants was investigated in two growing seasons (2015 and 2016). Plants of the cultivar ‘Silver and Gold’ were transplanted into pots either with added mycorrhizal inoculum or without inoculum and assigned to four phosphorus treatments. Mycorrhizal colonization was assessed by evaluating the frequency of colonization, intensity of colonization and density of fungal structures (arbuscules, vesicles, coils and microsclerotia) in the roots. During the growing season, the content of plant available phosphorus in the soil was analyzed, and shoot length, number of shoots, number of inflorescences, number of flowers and flowering time were evaluated. Inoculated Ajania plants were successfully colonized with arbuscular mycorrhizal fungi and dark septate endophytic fungi. In the root segments, hyphae were mainly observed, as well as vesicles, coils, arbuscules and microsclerotia, but in lower density. The density of fungal structures did not differ among phosphorus treatments, but did differ between years, with a higher density of fungal structures in 2016. Mycorrhizal plants developed higher number of shoots in 2016, higher number of inflorescences, higher number of flowers, and they flowered longer compared to uninoculated plants.

scholarly journals VEGETATIVE RESCUE AND ROOTING OF CUTTINGS OF DIFFERENT STOCK PLANTS OF Sequoia sempervirens

CERNE ◽  
2017 ◽  
Vol 23 (4) ◽  
pp. 435-444 ◽  
Author(s):  
Mariane de Oliveira Pereira ◽  
Alessandro Camargo Ângelo ◽  
Marcio Carlos Navroski ◽  
Mario Dobner Júnior ◽  
Luciana Magda de Oliveira
Keyword(s):  
Genetic Effect ◽  
Sequoia Sempervirens ◽  
Stem Cuttings ◽  
Fast Growing ◽  
Rooting Of Cuttings ◽  
Living Species ◽  
Recovered Material ◽  
Over 40 Years ◽  
Number Of Shoots

ABSTRACT Sequoia is a fast-growing, long-living species, producing durable timber. The aim of this study was to test different methods for the vegetative rescue of Sequoia sempervirens trees over 40 years old, made at different periods of the year, and, later, testing the rooting of individualized cuttings in planned arrays. Twenty-four individual sequoias were rescued, applying girdling and semi-girdling at three different heights (-10, 0, and 30 cm). The first collection was made 90 days after application of the treatments, being repeated at 150, 240, and 360 days. The percentage of budding trees and the number of shoots per array were registered. In all collections, shoots produced cuttings, which were placed for rooting in mini-tunnels. Cutting survival (%), rooting (%), and number of roots were registered, per array, and per collection. The species vegetative rescue proved to be efficient for the production of shoots for stem cuttings, especially with girdling at 30 cm and semi-girdling at -10 cm. However, it is difficult to define the best method, mainly because of the genetic effect among stock plants. The rooting of cuttings, of recovered material, presented good results (average >65%), also with great differences among stock plants. The potential for rooting of cuttings varied according to different planting periods, with high rooting rates in all seasons, especially in summer. Sequoia sempervirens shows the potential for vegetative rescue and cloning by rooting of cuttings, and this may lead to new studies, with a view towards fixing clones.

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals PENGARUH JUMLAH MATA TUNAS BATANG ATAS DAN TINGGI BATANG BAWAH PADA SAMBUNG PUCUK TERHADAP PERSENTASE TUMBUH JAMBU AIR (Syzygium Samarangense)

2020 ◽  
Vol 7 (2) ◽  
pp. 99
Author(s):  
Supriyono Supriyono ◽  
Tarwa Mustopa ◽  
Nunuk Helilusiatiningsih ◽  
Fristama Maulana
Keyword(s):  
Significant Interaction ◽  
Treatment Combination ◽  
Design Factor ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Shoots

Water guava (Syzygium Samarangense) was a popular plant in Indonesia because it was fresh and sweet. The aim of this research was to analyze the effect of the number of shoots of the upper stems and height of the rootstock at the shoot graft on the percentage of growing water guava. The method used in this study was a completely randomized design, factor into one rootstock length, namely 10 cm, 15 cm, and 20 cm. and the second factor was the number of buds on the scion, namely 2, 3, and 4. The results showed that there was a significant interaction in the treatment combination of the number of 4 buds on the upper stem and the length of the rootstock 15 cm at 35 days after grafting affected the growth percentage and growth in the number of leaves and length of the scion.

Sign in / Sign up

Export Citation Format

Share Document