scholarly journals Protection of Renal Function ByHyperbaric OxygenDuring Plasmodium berghei ANKA Infection

2020 ◽  
Vol 13 (1) ◽  
pp. 69-74
Author(s):  
Prawesty Diah Utami ◽  
Herin Setianingsih
Keyword(s):  
Renal Function ◽  
Control Group ◽  
Group 4 ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
Group 5 ◽  
Major Factors ◽  
High Level

Background:Malaria cases have consistently increased and the number of deaths remains largely unchanged.Malaria associated renal injury has a high level of morbidity and mortality. High level of blood urea nitrogen / BUN and plasma creatinine is one of the major factors associated with mortality in humans infected with malaria, indicating that impairment of renal function. HBO is widely used as an adjunctive therapy for many diseases, it is known that HBO hasan action as antiplasmodium, antiinflammation and antioxidant effects. Objective: The aim of this study was to determine the effect of HBO on BUN and creatinine levels in rats infected with P. berghei ANKA. Methods:This research was conducted experimentally post-test only control group on six groups of rats. The samples used were 24 male Rattus norvegicus wistar strain that have been infected by Plasmodium bergheiANKA and divided into 6 groups. Group 1 was given combination artesunate and 1, 5 ATA HBO, grup 2 was given combination artesunate and 3, 0 ATA HBO, group 3 was given 1, 5 ATA HBO, group 4 was given 3, 0 ATA HBO, group 5 was given artesunate and group 6 was given aquadest. HBO therapy is carried out for 10 days and the observation of the levels of BUN and creatinine in mice after treatment on tenth day. Results:Descriptive analysis and statistical analysis (Kruskal wallis snd Mann whitney U posthoc) showed a significant difference (p < α = 0.05) on mean levels of BUN and creatinine group receiving combination of artesunate and HBO 3,0 ATA compared to other groups.Hyperbaric oxygen has the effect of reducing the levels of BUN and creatinine in rats infected by P.berghei ANKA.

scholarly journals Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

2012 ◽  
Vol 6 (6) ◽  
pp. 234 ◽  
Author(s):  
Ugur Kuyumcuoglu ◽  
Bilal Eryildirim ◽  
Murat Tuncer ◽  
Gokhan Faydaci ◽  
Fatih Tarhan ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Group 4 ◽  
Double J Stent ◽  
Urinary Tract Symptoms ◽  
Stone Treatment ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

scholarly journals Zingerone Ameliorates Hepatotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 280-284 ◽  
Author(s):  
Julietpoornamathy J ◽  
Parameswari C.S.
Keyword(s):  
The Body ◽  
Control Group ◽  
Medical Sciences ◽  
Toxic Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Insight Into ◽  
Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats.  A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

scholarly journals Comparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial

2018 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
Hatice Miray Uyan ◽  
Keziban Olcay ◽  
Mutlu Özcan
Keyword(s):  
Postoperative Pain ◽  
Repeated Measures ◽  
Control Group ◽  
Periapical Lesions ◽  
Single Visit ◽  
Group 4 ◽  
Multiple Visit ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

<p><strong>Objective:</strong> This study was designed to evaluate postoperative pain after endodontic retreatment. <strong>Material and Methods:</strong> Asymptomatic, multi-rooted molar &amp; premolar teeth requiring retreatment with 2–5 mm periapical lesions were included. Seventy-eight teeth were randomly placed in four groups (n=20): single-visit (control, group 1), Ledermix (group 2), metronidazole, ciprofloxacin, minocycline mixture (group 3), calcium hydroxide (group 4). The postoperative pain was recorded using a VAS at 6, 12, 24, and 48 h after retreatment. Statistical evaluation was performed using Two-Way Repeated Measures ANOVA and Tukey test. <strong>Results:</strong> Mild pain occurred in 67.5%, moderate in 30%, and flare-ups in 2.5%, and there was a significant difference between the groups (p&lt;0.01). Significantly lower postoperative pain was observed in TAP and CaOH<sub>2 </sub>groups(p&lt;0.05). In the 6, 12, and 24 h intervals, there was a significant difference in the pain levels (p&lt;0.05). <strong>Conclusion:</strong> TAP and CaOH<sub>2</sub> are effective for reducing postoperative pain after retreatment.</p><p><strong>Keywords</strong></p><p>Intracanal medicaments; Multiple-visit; Postoperative pain; Retreatment; Single-visit.</p>

In vivosilymarin’s antioxidant and anti-apoptotic effects on photodynamic therapy’s responsiveness

2017 ◽  
Vol 21 (03) ◽  
pp. 189-197 ◽  
Author(s):  
Vlad Dionisie ◽  
Simona Clichici ◽  
Rodica M. Ion ◽  
Oana O. Danila ◽  
Remus Moldovan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Reactive Oxygen Species Generation ◽  
Group 4 ◽  
Significant Difference ◽  
Anti Oxidant ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apoptotic Effects ◽  
Oxidant Effect

Several studies have shown that some anti-oxidant natural compounds in combination with photodynamic therapy (PDT) can enhance the effectiveness of treatment. The aim of this study is to evaluate the effect of silymarin (SIL) in combination with 5,10,15,20-tetra-sulphonato-phenyl-porphyrin (TSPP) based photodynamic therapy, on experimental tumors. 30 Wistar rats with Walker carcinosarcoma, were divided into 6 groups: group 0 (control) — control, untreated group; group 1 (TSPP) — one dose of TSPP; group 2 (SIL) — silymarin; group 3 (PDT) — TSPP and irradiation 24 h after; group 4 (SIL[Formula: see text]PDT) — silymarin, TSPP and irradiation 24 h after; group 5 (SIL[Formula: see text]IR) and group 6 (IR) — irradiation and in addition, group 5 received SIL. Silymarin administered before photodynamic therapy decreased the lipid peroxidation ([Formula: see text] < 0.05) and modulated the antioxidant defense in tumor treated with PDT and silymarin suggesting that silymarin administration along with photodynamic therapy has an anti-oxidant effect. The caspase — 8 level and -3 activity increased in PDT and PDT [Formula: see text] SIL groups compared to the control; between the two groups there was a significant difference in term of apoptosis in favor to PDT. In conclusion, silymarin administration inhibited the reactive oxygen species generation and reduced the tumoral cells’ apoptosis, suggesting that natural compound administered before photodynamic therapy did not improve the therapy’s effect.

Efficacy of Double Manual Cleaning Versus Automated Cleaning for Removal of Biofilm of Hinged Surgical Instruments

2020 ◽  
Vol 41 (S1) ◽  
pp. s518-s519
Author(s):  
Dayane Costa ◽  
Roel Castillo ◽  
Lillian Kelly Lopes ◽  
Anaclara Tipple ◽  
Honghua Hu ◽  
...  
Keyword(s):  
Protein Level ◽  
Positive Control ◽  
Surgical Instruments ◽  
Microbial Load ◽  
Positive Control Group ◽  
Control Group ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

Objectives: To evaluate the efficacy of double manual cleaning (DMC) with enzymatic followed by alkaline detergent for removing biofilm on hinged surgical instruments compared to automated cleaning by the washer-disinfector. Methods: Biofilm of Staphylococcus aureus (ATCC 25923) was formed in vitro on hemostatic forceps (Fig. 1). Biofilm-covered forceps were rinsed in distilled water and subjected to one of the following cleaning regimes (n = 5 forceps each): Group 1 forceps were soaked in sterile water for 5 minutes. Group 2-DMC forceps were soaked in enzymatic detergent, brushed 5 times on each face, rinsed with filtrated water (0.2 µm), soaked in alkaline detergent, brushed 5 times each face, rinsed with filtrated water (0.2 µm), and dried with sterile cloth. For group 3-DMC plus hinge inner brushing (n = 5), the forceps were soaked in detergents and brushed as in group 2, including hinge inner brushing (2-mm lumen brush) (Fig. 1). In group 4 (automated cleaning in a washer/disinfector), forceps were prewashed, washed once, washed again, rinsed, thermally rinsed, and dried. After the treatments, forceps were evaluated for microbial load (counting of colony-forming units), residual protein (BCA protein assay kit), and biofilm (scanning electron microscopy). Results: There was no statistically significant differences between the microbial load and protein level contaminating the forceps subjected to DMC (group 2) and the positive control group. The DMC with hinge inner brushing group (group 3) and the automated cleaning group (group 4) demonstrated a significantly reduced microbial load: reduction averages of 2.8 log 10 (P = .038) and 7.6 log10 (P ≤ .001), respectively. The protein level remaining on the forceps also significantly decreased: 2.563 μg (P = .016) and 1,453 μg (P = .001), respectively, compared to the positive control group. There was no statistically significant difference between DMC with hinge inner brushing and automated cleaning (groups 3 and 4) for all of the tests performed. None of the cleaning methods completely removed biofilm and/or soil from the forceps hinge internal region (Fig. 1). Conclusions: Automated cleaning had the best efficacy for removing biofilm. However, DMC with hinge inner brushing was an acceptable alternative cleaning method for sterilizing service units with only manual cleaning available, as is the case in most low- and middle-income countries. Neither automated nor any manual cleaning regimes were able to completely remove biofilm and soil from the forceps hinged area, and the amount of protein left after automated and DMC plus hinge brushing was higher than the recommended. Cleaning is the most important step for the reprocessing of reusable medical devices; thus, efforts must be undertaken to improve cleaning in different social and economic realities and scenarios.Funding: This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES.Disclosures: None

scholarly journals FE-SEM Evaluation of Dental Specimens Prepared by Different Methods forIn VitroContamination

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Vitor Cesar Nakamura ◽  
Simony Hidee Hamoy Kataoka ◽  
Giulio Gavini ◽  
Patrícia Helena Ferrari ◽  
Silvana Cai
Keyword(s):  
Control Group ◽  
Buffer Solution ◽  
Morphological Alterations ◽  
Ultrasonic Bath ◽  
Group 4 ◽  
Cement Removal ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Objective. To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation.Methods. Twenty-five human single-rooted teeth were used. The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (), according to the preparation methods employed—control group: no solutions applied; group 1: cement removal and irrigation with 5.25% % EDTA for 4 minutes each; group 2: 17%  % NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17%     buffer solution + distilled water (10 minutes ultrasonic); group 4: 17%   NaOCl (3 minutes ultrasonic bath). Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification. Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test.Results. In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4.Conclusion. The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.

scholarly journals The Evaluation of Various Restoration Techniques on Internal Adaptation of Composites in Class V Cavities

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
D. Dionysopoulos ◽  
C. Papadopoulos ◽  
E. Koliniotou-Koumpia
Keyword(s):  
Room Temperature ◽  
Control Group ◽  
Class V ◽  
Group 4 ◽  
Restoration Techniques ◽  
Group 3 ◽  
Internal Adaptation ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim.The aim of this study was to evaluate the effect of different restoration techniques on the formation of internal microgaps between materials and dentin in class V restorations.Materials and Methods.Twenty-five extracted human premolars were prepared with standardized class V cavity outlines (3 mm × 2 mm × 2 mm). The cavities were randomly divided into 5 groups of 10 cavities each and restored according to manufacturer’s instructions: Group 1: preheating (55°C) conventional composite (Filtek Z250), Group 2: flowable composite (Filtek Flow), Group 3: Filtek Flow + Filtek Z250 light-cured separately, Group 4: Filtek Flow + Filtek Z250 light-cured simultaneously, and Group 5 (control): Filtek Z250 at room temperature (23°C). The specimens were then thermocycled and cross-sectioned through the center of the restoration. Subsequently, impressions were taken, and epoxy resin replicas were made. The internal adaptation of the materials to the axial wall was analyzed under SEM.Results.The preheated Filtek Z250 (Group 1) showed better internal adaptation than the room temperature groups(P<0.05). The combination of Filtek Flow with Filtek Z250 which was light-cured separately (Group 3) exhibited better internal adaptation than control group(P<0.05).Conclusion.Different restoration techniques exhibit different behavior regarding internal adaptation to dentin after photopolymerization.

scholarly journals Effect of Different Finishing and Polishing Methods for Color Stability of the E-Max Dental Ceramics: In vitro Study

2021 ◽  
Vol 14 (4) ◽  
pp. 1508-1513
Author(s):  
Ibraheem F Alshiddi
Keyword(s):  
Surface Brightness ◽  
Color Stability ◽  
Color Measurement ◽  
Control Group ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

In order to assess the influence of finishing and polishing on the surface brightness and color stability of the ceramic veneer, fifty specimens were fabricated with 10 mm diameter and 2 mm thickness using IPS E-Max Ceramic. After glazing, 10 specimens were untouched as control group, and the other 40 specimens were abraded using 125µm diamond bur to create surface roughness. Forty specimens were divided into four groups (n=10), in group 1: specimens were finished using diamond point, in group 2 specimens’ surface was polished with a polishing kit, Group 3: Each specimen surface was polished with the polishing kit as in protocol 2 and was polished a polishing past and group 4 Each specimen was glazed by heating at 621℃ for 3 minutes followed by a temperature increase of 83℃/min up to 918℃ for 30 seconds. Color measurement was performed using spectrophotometer. Color stability data were analyzed using two-way ANOVA and Tukey’s HSD test (α=0.05). For Ra values, paired-samples t-tests were used to analyze the data and compare groups. The change in L and E showed a significant difference among the study groups; (group 1, group 2, group 3 and group 4) with respect to three variables L, a and b. A significant difference was noted when compared each group with the control; however, only group 2 showed a significant difference from group 4; the remaining groups demonstrated similar findings for all three variables. The study displayed a significant impact of the finishing and polishing technique on the surface brightness and color stability of ceramic restoration. However, it was evident that combination of two or three polishing techniques which includes polish kit and glaze enhances the surface finish and adds color stability by alternating the yellow – blue axis (increase in b) and red- green axis (decrease in a).

scholarly journals Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwi Retna Prihati
Keyword(s):  
Body Weight ◽  
Test Group ◽  
Laboratory Research ◽  
Control Group ◽  
Female Rat ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

scholarly journals Effect of per oral sipermetrin exposure on serum 17 estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

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