scholarly journals Effect of per oral sipermetrin exposure on serum 17 estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

scholarly journals Effect of per oral sipermetrin exposure on serum 17-beta estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

scholarly journals The differences of glycodelin and uterus NK cell expression in obese and non-obese rats (Rattus norvegicus)

2021 ◽  
Vol 29 (2) ◽  
pp. 63
Author(s):  
Diyah Nofita Ofa Ningtriyas ◽  
Arsana Wiyasa ◽  
Muhammad Nooryanto
Keyword(s):  
Rattus Norvegicus ◽  
Nk Cell ◽  
Female Rats ◽  
White Rats ◽  
Wistar Strain ◽  
Obese Female ◽  
Significant Difference ◽  
Obese Rats ◽  
Cell Expression

HIGHLIGHTS1. Obesity increases the risk of comorbidities especially for the pregnancy.2. The study analyzed glycodelin levels and uterine NK cell expression using Rattus norvegicus as animal model.3. uNK cell expression of the obese rats group was higher as the marker of chronic inflammation for obesity.4. Although there was increasing uNK cells in obese rats group, this result was not followed by the level of gycodelin.ABSTRACTObjectives: To prove the existence of differences in glycodelin levels and uterine NK cell expression in obese and non-obese female white rats of Wistar strain (Rattus norvegicus).Materials and Methods: . This study used a randomized post-test only controlled group design. This in vivo study used two groups of female rats (Rattus norvegicus). Group 1 was treated with the high obese diet for eight weeks, and group 2 was not treated with the high obese diet. After eight weeks, the rats were weighed, the proestrus phase was synchronized, and then the rats were terminated.Results: In this study, there was no significant difference in glycodelin levels between the obese and non-obese groups with a p= 0.821 (p >0.05). Significant differences were found in uterine NK cell expression between obese dan non-obese groups with p=0.001 (p <0.05). The correlation test of glycodelin levels and uterine NK cell expression showed insignificant results with a correlation coefficient of 0.120 and p=0.513. This proved that there was no significant correlation between glycodelin levels and uterine NK cell expression.Conclusion: There was no significant difference between glycodelin levels and uterine NK cell expression in obese and non-obese female white rats of Wistar strain (Rattus norvegicus). 

scholarly journals The Effect of Medroxy Progesterone Acetate Hormone Administration to Endometrial Thickness of the Female Rattus norvegicus Wistar strain

2020 ◽  
Vol 4 (2) ◽  
pp. 94
Author(s):  
Devi Yulita
Keyword(s):  
Rattus Norvegicus ◽  
Endometrial Thickness ◽  
Female Rats ◽  
Control Group ◽  
Gonadotropin Secretion ◽  
Control Group Design ◽  
White Rats ◽  
Wistar Strain ◽  
Post Test

Many alternative contraceptives can be used as needed. The most effective type of contraception is injection contraception because it is relatively safe, effective, simple and inexpensive. Contraceptive users must be selective in choosing contraception because there is no method of contraception that is safe and effective for all acceptors. The side effects of Medroxy Progesterone Acetat injection include bleeding disorders such as amenorrhoea, weight gain, high blood pressure, acne, spotting. Medroxy Progesterone Acetat is a progestin contraceptive regimen that is often used and works long-term. The mechanism of action of Medroxy Progesterone Acetate injection is to inhibit gonadotropin secretion so that it will prevent the maturation of primary follicles in the ovary and also prevent ovulation and cause endometrial depletion.The research design is the post test only control group design. The population in this research  are the white rats species Rattus norvegicus Wistar strain. The samples are taken randomly from all populations that met the following sample criteria: Female rats, Aged 12-14 weeks, having a body weight of 150 - 200 grams.The results showed that Medroxy Progesterone Acetate hormone affects the thickness of the endometrium, and there is a difference in the histological picture of endometrial thickness due to the administration of the Medroxy Progesterone Acetate hormone and without the administration of the Medroxy Progesterone Acetate hormone

scholarly journals Pengaruh Pemberian Hormon Medroxy Progesterone Acetat terhadap Ketebalan Endometrium Rattus Norvegicus Betina Strain Wistar

2020 ◽  
Vol 20 (2) ◽  
pp. 682
Author(s):  
Defi Yulita ◽  
M Biomed
Keyword(s):  
Family Planning ◽  
Treatment Group ◽  
Sample Size ◽  
Rattus Norvegicus ◽  
Endometrial Thickness ◽  
Female Rats ◽  
Control Group ◽  
Childbearing Age ◽  
Wistar Strain ◽  
Significant Difference

Based on the National Population and Family Planning Agency (BKKBN) data in 2013, there were 8,500,247 couples of childbearing age who were new KB (family planning) participants with the total number using injection was 4,128,115 (48.56%). Research Objectives to find out The Effect of The Administration OF Medroxy Progesterone Acetate Hormone to Endometrial Thickness of The Female Rattus Norvegicus Wistar Strain. The design of this research is the post test only control group design by having  white rats species Rattus Norvegicus Wistar Strain as the poplulation, the samples are taken randomly from all populations that met the following sample criteria: female rats, Aged 12-14 weeks, having body weight of 150 - 200 grams. Based on the calculation of the sample size, the sample size / number of animals r = 10 rats was obtained, with a total sample size for both groups of treatment and control were 20 female rats which included: group 1 amounted to 10 rats that were not given Medroxy Progesterone Acetat hormones as a control group where group 2 amounted to 10 rats which given Medroxy Progesterone Acetat as a treatment group. The results showed the average endometrial thickness in the (P1) Medroxy Progesterone Acetate Hormone group was 493.17 µm ± 114.96, this result was lower than the control group 1192.56 µm ± 428.94. From the ANOVA test table, the P value <0.05 means that there is a very significant difference in the average endometrial thickness between the control group and the treatment group. Based on the results of the Post Hoc Bonfferoni Test, it can be seen that the average endometrial thickness between Control and P1 showed a significant difference (P <0.05). The analysis of the result and discussion of the research can be concluded that: the Medroxy Progesterone Acetate hormone affects the endometrial thickness, and there is a difference in the histological picture of endometrial thickness due to the administration of the Medroxy Progesterone Acetate hormone and without the administration of to the administration of the Medroxy Progesterone Acetate hormone.

Pengaruh Ekstrak Bawang Putih Terhadap Kadar Serum Kreatinin Tikus Hipertensi Two Kidney One Clipp (Effect of Allium sativum Extract to Serum Creatinine of Two Kidney One Clipp Hypertension Rat)

MEDULA ◽  
2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Sapto Raharjo ◽  
Gustavita Maria Bandong ◽  
Tien Tien ◽  
Andi Noor Kholidha Syarif ◽  
Agus Chahyadi ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Allium Sativum ◽  
Experimental Parameter ◽  
In Vitro Study ◽  
Control Group ◽  
Control Group Design ◽  
Wistar Strain ◽  
Post Test

Background: Garlic (Allium sativum) has long been used both as a food and traditional medicine. Several studies have shown  potential of garlic (Allium sativum) as an antihypertensive. In previous in vitro study found that dipeptide on garlic extract after hydrolyzed by the enzyme papain have ACE inhibitory activity. Induction of hypertension with 2 Kidney One Clip (2K1C) methodcauses the kidneys lose their physiological functions. A.sativum is expected to ameliorate kidney function. This study aimed to analyze creatinine levels in rat induced by 2K1C. Method: These study was an in vivo study with Pre-Post Test Only Control group Design. Before treatment, 30 subjects induced hypertension by 2K1C method. Treatment was conducted for four weeks. The recent study was conducted on 36 Rattus norvegicus, Wistar strain were divided into 6 groups. Measurement of creatinine was conducted by spectrophotometry method. Result: The results showed that there were significant differences in creatinine levels between groups (p < 0.05) both in the pre-test and post-test. The normal group and hypertension group did not have significant differences in creatinine pre- and post-test levels (p > 0.05). A.sativum 75m/kgBB gave the best decrease in creatinine levels up to 1.75 mg/dL compared to the A.sativum 25mg/kgBB and A.sativum group 50 mg/kgBB. There were significant differences in creatinine levels A.sativum 75 mg/kgBB and captopril (p <0.05). Conclusion: There was significant differences in creatinine levels of hypertensive rats between groups and the most effective dose was 75 mg / kg. Keywords: A.sativum, hypertension, creatinine, 2K1C Latar Belakang: Bawang putih (Allium sativum) telah lama digunakan, baik sebagai bahan masakan maupun sebagai obat tradisional. Beberapa studi menunjukkan potensi bawang putih (A. sativum) sebagai antihipertensi. Pada penelitian sebelumnya secara in vitro ditemukan bahwa dipeptida pada ekstrak bawang putih setelah dihidrolisis dengan enzim papain memiliki aktivitas inhibisi terhadap ACE. Induksi hipertensi 2 Kidney One Clip (2K1C) menyebabkan ginjal kehilangan fungsi fisiologisnya. Pemberian A.sativum  diketahui mampu memperbaiki fungsi ginjal. Penelitian ini bertujuan untuk menganalisis kadar kreatinin pada tikus yang diinduksi 2K1C. Metode: Penelitian ini merupakan jenis penelitian in vivo dengan menggunakan rancangan Control Group Time Series Experimental. Parameter dalam penelitian ini adalah tekanan darah. Sebelum perlakuan, subjek diinduksi hipertensi dengan metode 2K1C. Perlakuan dimulai pada minggu ke-enam setelah induksi. Perlakuan berlangsung selama empat minggu. Subjek yang digunakan dalam penelitian adalah tikus putih (Rattus norvegicus, strain Wistar), sebanyak 36 ekor dibagi dalam 6 kelompok: normal/sehat (tidak diinduksi), hipertensi yang diberi kaptopril, hipertensi yang diberi ekstrak A.sativum 25mg/kgBB, hipertensi yang diberi ekstrak A. sativum 50mg/kgBB, hipertensi yang diberi ekstrak A. sativum 75mg/kgBB, dan hipertensi tanpa perlakuan. Pengukuran kreatinin dilakukan menggunakan metode spektrofotometri. Hasil: Analisis statistik menunjukkan terdapat perbedaan kadar kreatinin signifkan pada setiap kelompok (p < 0.05) baik pre-test maupun post-test. Kelompok normal dan hipertensi tidak memiliki perbedaan signifikan pada kadar kreatinin pre dan post-test (p > 0.05). Kelompok A. sativum 75m/kgBB menunjukkan penurunan kadar kreatinin yang sangat signifikan hingga 1.75 mg/dL dibanding kelompok  A. sativum 25mg/kgBB and A. sativum group 50 mg/kgBB. Terdapat perbedaan kadar kreatinin yang signifikan antara kelompok A. sativum 75 mg/kgBB dan captopril (p <0.05). Simpulan: Terdapat perbedaan kadar kreatinin yang signifikan antar kelompok dan penurunan kadar kreatinin terbaik yaitu pada dosis  75 mg / kg. Kata Kunci: A.sativum, hipertensi, kreatinin, 2K1C

scholarly journals Dosis Efektif Enoxaparin dalam Mencegah Terjadinya Trombosis pada Anastomosis Arteri Femoralis Tikus

2017 ◽  
Vol 1 (2) ◽  
pp. 34
Author(s):  
I Made Suka Adnyana ◽  
Iswinarno Doso Saputro
Keyword(s):  
Free Flap ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Wistar Strain ◽  
Post Test

Tujuan: untuk mengetahui dosis efektif enoxaparin dalam mencegah terjadinya trombosis pada anastomosis mikrovaskular. Metode: penelitian ini bersifat eksperimental dengan rancangan the randomized post test only control group design. Terdapat 33 tikus jantan Rattus norvegicus Wistar strain yang dikelompokkan menjadi tiga perlakuan yaitu perlakuan A (enoxaparin 0,75 mg/kg), B (enoxaparin 1  mg/kg), dan C (kontrol). Tuck model anastomosis dilakukan pada arteri femoralis, kemudian luas trombus yang terjadi pada pembuluh darah dibandingkan dengan diameter lumen pembuluh darah diukur dengan graticule lens dan dinyatakan dalam persen. Hasil: trombus terbentuk pada semua subyek penelitian baik pada kelompok perlakuan maupun kontrol. Rerata persentase luas trombus pada kelompok enoxaparin 0,75 mg/kg adalah 24,3%, enoxaparin 1 mg/kg sebesar 19,8% dan kelompok NaCl 0,9% sebesar 79,4%. Terdapat perbedaan antara perlakuan pemberian enoxaparin dosis 0,75 mg/kg dan 1 mg/kg dengan kontrol, namun tidak ada perbedaan bermakna rerata persentase luas trombus diantara kelompok enoxaparin dosis 0,75 mg/kg dan dosis 1 mg/kg (p=0,624). Perlu dilakukan penelitian secara klinis guna melihat efektivitas enoxaparin dalam meningkatkan patensi anastomosis pada free flap maupun replantasi. Simpulan: pemberian enoxaparin dosis 0,75 mg/kg dan enoxaparin dosis 1 mg/kg secara subkutan efektif mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus. Tidak terdapat perbedaan efektivitas yang bermakna dalam mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus setelah diberikan dosis enoxaparin 0,75 mg/kg dan 1 mg/kg secara subkutan.

scholarly journals Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwi Retna Prihati
Keyword(s):  
Body Weight ◽  
Test Group ◽  
Laboratory Research ◽  
Control Group ◽  
Female Rat ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

scholarly journals Protection of Renal Function ByHyperbaric OxygenDuring Plasmodium berghei ANKA Infection

2020 ◽  
Vol 13 (1) ◽  
pp. 69-74
Author(s):  
Prawesty Diah Utami ◽  
Herin Setianingsih
Keyword(s):  
Renal Function ◽  
Control Group ◽  
Group 4 ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
Group 5 ◽  
Major Factors ◽  
High Level

Background:Malaria cases have consistently increased and the number of deaths remains largely unchanged.Malaria associated renal injury has a high level of morbidity and mortality. High level of blood urea nitrogen / BUN and plasma creatinine is one of the major factors associated with mortality in humans infected with malaria, indicating that impairment of renal function. HBO is widely used as an adjunctive therapy for many diseases, it is known that HBO hasan action as antiplasmodium, antiinflammation and antioxidant effects. Objective: The aim of this study was to determine the effect of HBO on BUN and creatinine levels in rats infected with P. berghei ANKA. Methods:This research was conducted experimentally post-test only control group on six groups of rats. The samples used were 24 male Rattus norvegicus wistar strain that have been infected by Plasmodium bergheiANKA and divided into 6 groups. Group 1 was given combination artesunate and 1, 5 ATA HBO, grup 2 was given combination artesunate and 3, 0 ATA HBO, group 3 was given 1, 5 ATA HBO, group 4 was given 3, 0 ATA HBO, group 5 was given artesunate and group 6 was given aquadest. HBO therapy is carried out for 10 days and the observation of the levels of BUN and creatinine in mice after treatment on tenth day. Results:Descriptive analysis and statistical analysis (Kruskal wallis snd Mann whitney U posthoc) showed a significant difference (p < α = 0.05) on mean levels of BUN and creatinine group receiving combination of artesunate and HBO 3,0 ATA compared to other groups.Hyperbaric oxygen has the effect of reducing the levels of BUN and creatinine in rats infected by P.berghei ANKA.

scholarly journals Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto
Keyword(s):  
Rattus Norvegicus ◽  
Exercise Program ◽  
The Body ◽  
Control Group ◽  
Regular Exercise ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

INFLUENCE OF JAMU MADURA “EMPOT SUPER” ON THE VAGINAL EPITHELIUM THICKNESS OF WHITE MICE (Rattus norvegicus) – AN IN VIVO STUDY

2017 ◽  
Vol 2 (1) ◽  
pp. 47
Author(s):  
Anik Listiyana
Keyword(s):  
Rattus Norvegicus ◽  
Negative Control Group ◽  
Negative Control ◽  
Vaginal Epithelium ◽  
In Vivo Study ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Epithelium Thickness

<p><em>The aim of this research is to determine the influence of jamu Madura “Empot Super” (JMES) on the vaginal epithelium thickness of Rattus norvegicus in vivo. This research is kind of “true experimental-post test only control group design”. The rats were given drinking JMES once daily PS (Per-Sonde) for a month, then the vagina was taken to be sample for HE colouring. The sample was observed by the binocular microscope (100 times magnification) to identify the changes in the thickness of their vaginal epithelium. Calculation of the vaginal epithelium thickness was counted on the 10 field of view chosen randomly by the blind method. The result show that the vaginal epithelium thickness increased with dose 0,17mg/BW, 0,34mg/BW, and 0,68mg/BW of JMES compared with negative control group. But, the vaginal epithelium thickness decrease at the dose 0,51mg/BW compared with negative control group.</em></p><p> </p><p><strong>Keywords</strong><strong>: </strong>Jamu Madura “Empot Super” (JMES), vaginal epithelium thickness, white mice (<em>Rattus norvegicus</em>), In Vivo study</p>

Sign in / Sign up

Export Citation Format

Share Document