scholarly journals Treatment with DHA Improves Epidermal Eeratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models

2019 ◽  
Author(s):  
Tinghan Jia ◽  
Wu Qiao ◽  
Qifeng Yao ◽  
Wenhui Wu ◽  
Ken Kaku
Keyword(s):  
Topical Treatment ◽  
Barrier Function ◽  
Skin Diseases ◽  
Morphological Differentiation ◽  
Human Keratinocytes ◽  
Human Epidermis ◽  
Poly I:C ◽  
Skin Barrier Function ◽  
Mrna Levels ◽  
Reconstructed Human Epidermis

Atopic dermatitis (AD) is a chronic inflammatory skin disease, which can cause skin barrier function damaged. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect in deficient skin model, there is no study to investigate the effects of topical treatment with DHA in inflammatory reconstructed human epidermis (RHE) model. The effects of DHA on monolayer normal human epidermal keratinocyte (NHEK) cells were evaluated via CCK-8, qPCR and ELISA. The skin related barrier function was assessed by hematoxylin-eosin (HE) staining, western blot (WB), Immunohistofluorescence (IF) and ELISA in normal and inflammatory RHE models. DHA upregulated filaggrin and loricrin expression at mRNA levels in addition to suppress overexpression of TNF-α，IL-1α and IL-6 stimulated by poly I:C plus LPS (stimulation cocktail) in cultured NHEK cells. After topical treatment with DHA, cocktail induced inflammatory characteristics of skin diseases including barrier morphological, differentiation proteins and TSLP secretion, which were alleviated in RHE models. Supplementation with DHA can improved related barrier function and have anti-inflammation effects in monolayer keratinocytes and RHE models, which indicated that DHA may have a potential value for the treatment of inflammation-associate skin diseases.

scholarly journals Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3156
Author(s):  
Tinghan Jia ◽  
Wu Qiao ◽  
Qifeng Yao ◽  
Wenhui Wu ◽  
Ken Kaku
Keyword(s):  
Docosahexaenoic Acid ◽  
Topical Treatment ◽  
Barrier Function ◽  
Skin Diseases ◽  
Human Epidermis ◽  
Cell Counting ◽  
Skin Barrier Function ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epidermal Keratinocyte ◽  
Reconstructed Human Epidermis

Atopic dermatitis (AD) is a chronic inflammatory skin disease that can cause skin barrier function damage. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect on deficient skin models, no studies have investigated the effects of topical treatment with DHA in an inflammatory reconstructed human epidermis (RHE) model. The effects of DHA on monolayer normal human epidermal keratinocyte (NHEK) cells were evaluated using cell counting kit-8 (CCK-8), real-time quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA). The skin-related barrier function was assessed using hematoxylin–eosin (HE) staining, Western blot (WB), immunohistofluorescence (IF), and ELISA in normal and inflammatory RHE models. Docosahexaenoic acid upregulated filaggrin and loricrin expression at mRNA levels in addition to suppressing overexpression of tumor necrosis factor-α (TNF-α), interleukin-α (IL-1α), and interleukin-6 (IL-6) stimulated by polyinosinic–polycytidylic acid (poly I:C) plus lipopolysaccharide (LPS) (stimulation cocktail) in cultured NHEK cells. After topical treatment with DHA, cocktail-induced inflammatory characteristics of skin diseases, including barrier morphology, differentiation proteins, and thymic stromal lymphopoietin (TSLP) secretion, were alleviated in RHE models. Supplementation with DHA can improve related barrier function and have anti-inflammation effects in monolayer keratinocytes and RHE models, which indicates that DHA may have potential value for the treatment of inflammation-associated skin diseases.

scholarly journals β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Hyoung-Geun Kim ◽  
Ki Sun Kim ◽  
Minji Kim ◽  
Sang-Hwan Shin ◽  
Yeong-Geun Lee ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Polar Fraction ◽  
Poly I:C ◽  
Interleukin 4 ◽  
Skin Barrier Function ◽  
Mrna Levels ◽  
Alcohol Extract ◽  
Barrier Effects

AbstractSoil has been used for treatment of wound and skin diseases and for cosmetic purposes. Fusidium coccineum (FC) SA-1FC (Ascomycota) is a fungus found in nature, and its by-products are present in humid soils with plant humus. This study investigates the medium of fermented FC as a covering for all skin problems, including dryness, inflammation, and wounds. A preliminary study revealed that an alcohol extract of FC had a skin-enhancing effect, and thin-layer chromatography revealed a major component in a non-polar fraction. Here we identify a major compound isolated from a non-polar fraction as β-glucogallin. The mRNA levels of filaggrin and HAS3 are upregulated by FC and β-glucogallin treatment in keratinocytes and immortalized human keratinocytes cells. In addition, FC and β-glucogallin exert anti-inflammatory effects by suppressing expression of interleukin-4/poly(I:C)-induced chemokines and inflammatory cytokines. In fibroblasts, Hs68 cells, FC and β-glucogallin stimulate cell migration. These results suggest that FC and β-glucogallin can enhance skin barrier function.

scholarly journals Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

2019 ◽  
Vol 20 (17) ◽  
pp. 4289 ◽  
Author(s):  
Ye-On Jung ◽  
Haengdueng Jeong ◽  
Yejin Cho ◽  
Eun-Ok Lee ◽  
Hye-Won Jang ◽  
...  
Keyword(s):  
Barrier Function ◽  
Lactobacillus Rhamnosus ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Main Function ◽  
Lauryl Sulfate ◽  
Protective Effects ◽  
Immunofluorescence Analysis ◽  
Reconstructed Human Epidermis

The main function of the skin is to protect the body from the external environment. The barrier function of the skin is mainly provided by the stratum corneum, which consists of corneocytes bound with the corneodesmosomes and lamellar lipids. Skin barrier proteins like loricrin and filaggrin also contribute to the skin barrier function. In various skin diseases, skin barrier dysfunction is a common symptom, and skin irritants like detergents or surfactants could also perturb skin barrier function. Many efforts have been made to develop strategies to improve skin barrier function. Here, we investigated whether the microfluidized lysates of Lactobacillus rhamnosus (LR), one of the most widely used probiotic species for various health benefits, may improve the skin barrier function in a reconstructed human epidermis, Keraskin™. Application of LR lysate on Keraskin™ increased the expression of tight junction proteins; claudin 1 and occludin as determined by immunofluorescence analysis, and skin barrier proteins; loricrin and filaggrin as determined by immunohistochemistry and immunofluorescence analysis and qPCR. Also, the cytotoxicity of a skin irritant, sodium lauryl sulfate (SLS), was alleviated by the pretreatment of LR lysate. The skin barrier protective effects of LR lysate could be further demonstrated by the attenuation of SLS-enhanced dye-penetration. LR lysate also attenuated the destruction of desmosomes after SLS treatment. Collectively, we demonstrated that LR lysate has protective effects on the skin barrier, which could expand the utility of probiotics to skin-moisturization ingredients.

Assessment of the Skin Barrier Function in the Reconstructed Human Epidermis using a Multimodal Approach at molecular, tissue and functional levels

The Analyst ◽  
2021 ◽  
Author(s):  
Joudi bakar ◽  
Rime Michael-Jubeli ◽  
Rindala El Khoury ◽  
Sabrina Hamla ◽  
Ali ASSI ◽  
...  
Keyword(s):  
Barrier Function ◽  
Functional Characterization ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Multimodal Approach ◽  
Reconstructed Human Epidermis

Reconstructed human epidermis models are used as epidermis alternatives in skin researches. It is necessary to provide molecular and functional characterization in order to assess these models. Our aim is...

scholarly journals Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 300 ◽  
Author(s):  
Dong Ju Son ◽  
Jae Chul Jung ◽  
Yong Min Choi ◽  
Hyeon Yeol Ryu ◽  
Somin Lee ◽  
...  
Keyword(s):  
Oral Administration ◽  
Barrier Function ◽  
Collagen Synthesis ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Water Evaporation ◽  
Skin Barrier Function ◽  
Type I ◽  
Skin Damage ◽  
Hairless Mice

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

scholarly journals Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1041
Author(s):  
Yuri Dancik ◽  
Hichem Kichou ◽  
Christophe Eklouh-Molinier ◽  
Martin Soucé ◽  
Emilie Munnier ◽  
...  
Keyword(s):  
Barrier Function ◽  
Principal Component ◽  
Human Epidermis ◽  
Animal Tissues ◽  
Reconstructed Human Epidermis ◽  
Percutaneous Permeation ◽  
Time Required ◽  
Kinetics Of ◽  
Permeation Kinetics

The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages. RHEs remain costly, however, due to consumables and time required for their culture and a short shelf-life. Storing, i.e., freezing RHE could help reduce costs but to date, little is known on the effects of freezing on the barrier function of RHE. We studied such effects using commercial EpiSkin™ RHE stored at −20, −80 and −150 °C for 1 and 10 weeks. We acquired intrinsic Raman spectra in the stratum corneum (SC) of the RHEs as well as spectra obtained following topical application of resorcinol in an aqueous solution. In parallel, we quantified the effects of freezing on the permeation kinetics of resorcinol from time-dependent permeation experiments. Principal component analyses discriminated the intrinsic SC spectra and the spectra of resorcinol-containing RHEs, in each case on the basis of the freezing conditions. Permeation of resorcinol through the frozen RHE increased 3- to 6-fold compared to fresh RHE, with the strongest effect obtained from freezing at −20 °C for 10 weeks. Due to the extensive optimization and standardization of EpiSkin™ RHE, the effects observed in our work may be expected to be more pronounced with other RHEs.

Reconstructed human epidermis: A model to study the barrier function

2005 ◽  
Vol 231 (1-4) ◽  
pp. 286-291 ◽  
Author(s):  
Y. Barbotteau ◽  
E. Gontier ◽  
P. Barberet ◽  
M. Cappadoro ◽  
B. De Wever ◽  
...  
Keyword(s):  
Barrier Function ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals Impact of Water Exposure and Temperature Changes on Skin Barrier Function

2022 ◽  
Vol 11 (2) ◽  
pp. 298
Author(s):  
Manuel Herrero-Fernandez ◽  
Trinidad Montero-Vilchez ◽  
Pablo Diaz-Calvillo ◽  
Maria Romera-Vilchez ◽  
Agustin Buendia-Eisman ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Diseases ◽  
Cold Water ◽  
Water Immersion ◽  
Skin Barrier ◽  
Hot Water ◽  
Skin Barrier Function ◽  
Inflammatory Skin Diseases ◽  
Water Exposure ◽  
The Impact

The frequency of hand hygiene has increased due to the COVID-19 pandemic, but there is little evidence regarding the impact of water exposure and temperature on skin. The aim of this study is to evaluate the effect of water exposure and temperature on skin barrier function in healthy individuals. A prospective observational study was conducted. Temperature, pH, transepidermal water loss (TEWL), erythema and stratum corneum hydration (SCH) were measured objectively before and after hot- and cold-water exposure and TempTest® (Microcaya TempTest, Bilbao, Spain) contact. Fifty healthy volunteers were enrolled. Hot-water exposure increased TEWL (25.75 vs. 58.58 g·h−1·m−2), pH (6.33 vs. 6.65) and erythema (249.45 vs. 286.34 AU). Cold-water immersion increased TEWL (25.75 vs. 34.96 g·h−1·m−2) and pH (6.33 vs. 6.62). TEWL (7.99 vs. 9.98 g·h−1·m−2) and erythema (209.07 vs. 227.79 AU) increased after being in contact with the hot region (44 °C) of the TempTest. No significant differences were found after contact with the cold region (4 °C) of the TempTest. In conclusion, long and continuous water exposure damages skin barrier function, with hot water being even more harmful. It would be advisable to use cold or lukewarm water for handwashing and avoid hot water. Knowing the proper temperature for hand washing might be an important measure to prevent flares in patients with previous inflammatory skin diseases on their hands.

scholarly journals Physiological Effects of Manganese Gluconate and Calcium Combination from Saint-Gervais Mont Blanc Spring Water for Human Keratinocytes Differentiation

2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Franck Juchaux ◽  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Spring Water ◽  
Keratinocyte Differentiation ◽  
Skin Barrier Function ◽  
Transglutaminase 1

Alterations of skin barrier function affect quality of life and there is a need to develop dermatological/cosmetic treatments to reinforce or restore it. Inspiring of Hailey-Hailey disease, in which barrier alteration is due to a mutation of a Calcium-transporting protein (ATP2C1), we focused on the role of minerals and more especially those contained in Saint-Gervais Mont Blanc (SGMB) spring water to reinforce barrier function. Objectives: Demonstrate the interest to enrich SGMB spring water with manganese to improve both keratinocytes differentiation and barrier function. Methods: Effects of treatments on the expression of ATP2C1 and on the expression of key markers in keratinocyte differentiation and barrier function were studied by gene expression analysis on keratinocytes monolayers and also by measuring the protein expression of transglutaminase 1 using in situ immunofluorescence and image analysis in keratinocytes monolayers. Results: SGMB spring water stimulates transcriptomic expression of key markers involved in keratinocytes differentiation and barrier function while manganese gluconate has no effect. Combination of both dramatically enhances keratinocytes differentiation, in a synergistic way, at both transcriptomic and protein level. None of treatments modulated ATP2C1 expression. Conclusion: These results highlight the interest to enrich SGMB spring water with manganese to boost keratinocytes differentiation and barrier function.

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