scholarly journals Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3156
Author(s):  
Tinghan Jia ◽  
Wu Qiao ◽  
Qifeng Yao ◽  
Wenhui Wu ◽  
Ken Kaku
Keyword(s):  
Docosahexaenoic Acid ◽  
Topical Treatment ◽  
Barrier Function ◽  
Skin Diseases ◽  
Human Epidermis ◽  
Cell Counting ◽  
Skin Barrier Function ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epidermal Keratinocyte ◽  
Reconstructed Human Epidermis

Atopic dermatitis (AD) is a chronic inflammatory skin disease that can cause skin barrier function damage. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect on deficient skin models, no studies have investigated the effects of topical treatment with DHA in an inflammatory reconstructed human epidermis (RHE) model. The effects of DHA on monolayer normal human epidermal keratinocyte (NHEK) cells were evaluated using cell counting kit-8 (CCK-8), real-time quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA). The skin-related barrier function was assessed using hematoxylin–eosin (HE) staining, Western blot (WB), immunohistofluorescence (IF), and ELISA in normal and inflammatory RHE models. Docosahexaenoic acid upregulated filaggrin and loricrin expression at mRNA levels in addition to suppressing overexpression of tumor necrosis factor-α (TNF-α), interleukin-α (IL-1α), and interleukin-6 (IL-6) stimulated by polyinosinic–polycytidylic acid (poly I:C) plus lipopolysaccharide (LPS) (stimulation cocktail) in cultured NHEK cells. After topical treatment with DHA, cocktail-induced inflammatory characteristics of skin diseases, including barrier morphology, differentiation proteins, and thymic stromal lymphopoietin (TSLP) secretion, were alleviated in RHE models. Supplementation with DHA can improve related barrier function and have anti-inflammation effects in monolayer keratinocytes and RHE models, which indicates that DHA may have potential value for the treatment of inflammation-associated skin diseases.

scholarly journals Treatment with DHA Improves Epidermal Eeratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models

2019 ◽  
Author(s):  
Tinghan Jia ◽  
Wu Qiao ◽  
Qifeng Yao ◽  
Wenhui Wu ◽  
Ken Kaku
Keyword(s):  
Topical Treatment ◽  
Barrier Function ◽  
Skin Diseases ◽  
Morphological Differentiation ◽  
Human Keratinocytes ◽  
Human Epidermis ◽  
Poly I:C ◽  
Skin Barrier Function ◽  
Mrna Levels ◽  
Reconstructed Human Epidermis

Atopic dermatitis (AD) is a chronic inflammatory skin disease, which can cause skin barrier function damaged. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect in deficient skin model, there is no study to investigate the effects of topical treatment with DHA in inflammatory reconstructed human epidermis (RHE) model. The effects of DHA on monolayer normal human epidermal keratinocyte (NHEK) cells were evaluated via CCK-8, qPCR and ELISA. The skin related barrier function was assessed by hematoxylin-eosin (HE) staining, western blot (WB), Immunohistofluorescence (IF) and ELISA in normal and inflammatory RHE models. DHA upregulated filaggrin and loricrin expression at mRNA levels in addition to suppress overexpression of TNF-α，IL-1α and IL-6 stimulated by poly I:C plus LPS (stimulation cocktail) in cultured NHEK cells. After topical treatment with DHA, cocktail induced inflammatory characteristics of skin diseases including barrier morphological, differentiation proteins and TSLP secretion, which were alleviated in RHE models. Supplementation with DHA can improved related barrier function and have anti-inflammation effects in monolayer keratinocytes and RHE models, which indicated that DHA may have a potential value for the treatment of inflammation-associate skin diseases.

scholarly journals Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

2019 ◽  
Vol 20 (17) ◽  
pp. 4289 ◽  
Author(s):  
Ye-On Jung ◽  
Haengdueng Jeong ◽  
Yejin Cho ◽  
Eun-Ok Lee ◽  
Hye-Won Jang ◽  
...  
Keyword(s):  
Barrier Function ◽  
Lactobacillus Rhamnosus ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Main Function ◽  
Lauryl Sulfate ◽  
Protective Effects ◽  
Immunofluorescence Analysis ◽  
Reconstructed Human Epidermis

The main function of the skin is to protect the body from the external environment. The barrier function of the skin is mainly provided by the stratum corneum, which consists of corneocytes bound with the corneodesmosomes and lamellar lipids. Skin barrier proteins like loricrin and filaggrin also contribute to the skin barrier function. In various skin diseases, skin barrier dysfunction is a common symptom, and skin irritants like detergents or surfactants could also perturb skin barrier function. Many efforts have been made to develop strategies to improve skin barrier function. Here, we investigated whether the microfluidized lysates of Lactobacillus rhamnosus (LR), one of the most widely used probiotic species for various health benefits, may improve the skin barrier function in a reconstructed human epidermis, Keraskin™. Application of LR lysate on Keraskin™ increased the expression of tight junction proteins; claudin 1 and occludin as determined by immunofluorescence analysis, and skin barrier proteins; loricrin and filaggrin as determined by immunohistochemistry and immunofluorescence analysis and qPCR. Also, the cytotoxicity of a skin irritant, sodium lauryl sulfate (SLS), was alleviated by the pretreatment of LR lysate. The skin barrier protective effects of LR lysate could be further demonstrated by the attenuation of SLS-enhanced dye-penetration. LR lysate also attenuated the destruction of desmosomes after SLS treatment. Collectively, we demonstrated that LR lysate has protective effects on the skin barrier, which could expand the utility of probiotics to skin-moisturization ingredients.

Assessment of the Skin Barrier Function in the Reconstructed Human Epidermis using a Multimodal Approach at molecular, tissue and functional levels

The Analyst ◽  
2021 ◽  
Author(s):  
Joudi bakar ◽  
Rime Michael-Jubeli ◽  
Rindala El Khoury ◽  
Sabrina Hamla ◽  
Ali ASSI ◽  
...  
Keyword(s):  
Barrier Function ◽  
Functional Characterization ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Multimodal Approach ◽  
Reconstructed Human Epidermis

Reconstructed human epidermis models are used as epidermis alternatives in skin researches. It is necessary to provide molecular and functional characterization in order to assess these models. Our aim is...

scholarly journals Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model

2021 ◽  
Vol 22 (23) ◽  
pp. 13091
Author(s):  
Andréa Tremblay ◽  
Mélissa Simard ◽  
Sophie Morin ◽  
Roxane Pouliot
Keyword(s):  
Docosahexaenoic Acid ◽  
Barrier Function ◽  
Culture Media ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Permeability ◽  
Skin Substitutes ◽  
Normal Human Skin ◽  
Assembly Method

Healthy skin moLEdels produced by tissue-engineering often present a suboptimal skin barrier function as compared with normal human skin. Moreover, skin substitutes reconstructed according to the self-assembly method were found to be deficient in polyunsaturated fatty acids (PUFAs). Therefore, in this study, we investigated the effects of a supplementation of the culture media with docosahexaenoic acid (DHA) on the barrier function of skin substitutes. To this end, 10 μM DHA-supplemented skin substitutes were produced (n = 3), analyzed, and compared with controls (substitutes without supplementation). A Franz cell diffusion system, followed by ultra-performance liquid chromatography, was used to perform a skin permeability to testosterone assay. We then used gas chromatography to quantify the PUFAs found in the epidermal phospholipid fraction of the skin substitutes, which showed successful DHA incorporation. The permeability to testosterone was decreased following DHA supplementation and the lipid profile was improved. Differences in the expression of the tight junction (TJ) proteins claudin-1, claudin-4, occludin, and TJ protein-1 were observed, principally a significant increase in claudin-1 expression, which was furthermore confirmed by Western blot analyses. In conclusion, these results confirm that the DHA supplementation of cell culture media modulates different aspects of skin barrier function in vitro and reflects the importance of n-3 PUFAs regarding the lipid metabolism in keratinocytes.

scholarly journals Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1041
Author(s):  
Yuri Dancik ◽  
Hichem Kichou ◽  
Christophe Eklouh-Molinier ◽  
Martin Soucé ◽  
Emilie Munnier ◽  
...  
Keyword(s):  
Barrier Function ◽  
Principal Component ◽  
Human Epidermis ◽  
Animal Tissues ◽  
Reconstructed Human Epidermis ◽  
Percutaneous Permeation ◽  
Time Required ◽  
Kinetics Of ◽  
Permeation Kinetics

The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages. RHEs remain costly, however, due to consumables and time required for their culture and a short shelf-life. Storing, i.e., freezing RHE could help reduce costs but to date, little is known on the effects of freezing on the barrier function of RHE. We studied such effects using commercial EpiSkin™ RHE stored at −20, −80 and −150 °C for 1 and 10 weeks. We acquired intrinsic Raman spectra in the stratum corneum (SC) of the RHEs as well as spectra obtained following topical application of resorcinol in an aqueous solution. In parallel, we quantified the effects of freezing on the permeation kinetics of resorcinol from time-dependent permeation experiments. Principal component analyses discriminated the intrinsic SC spectra and the spectra of resorcinol-containing RHEs, in each case on the basis of the freezing conditions. Permeation of resorcinol through the frozen RHE increased 3- to 6-fold compared to fresh RHE, with the strongest effect obtained from freezing at −20 °C for 10 weeks. Due to the extensive optimization and standardization of EpiSkin™ RHE, the effects observed in our work may be expected to be more pronounced with other RHEs.

Reconstructed human epidermis: A model to study the barrier function

2005 ◽  
Vol 231 (1-4) ◽  
pp. 286-291 ◽  
Author(s):  
Y. Barbotteau ◽  
E. Gontier ◽  
P. Barberet ◽  
M. Cappadoro ◽  
B. De Wever ◽  
...  
Keyword(s):  
Barrier Function ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals Impact of Water Exposure and Temperature Changes on Skin Barrier Function

2022 ◽  
Vol 11 (2) ◽  
pp. 298
Author(s):  
Manuel Herrero-Fernandez ◽  
Trinidad Montero-Vilchez ◽  
Pablo Diaz-Calvillo ◽  
Maria Romera-Vilchez ◽  
Agustin Buendia-Eisman ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Diseases ◽  
Cold Water ◽  
Water Immersion ◽  
Skin Barrier ◽  
Hot Water ◽  
Skin Barrier Function ◽  
Inflammatory Skin Diseases ◽  
Water Exposure ◽  
The Impact

The frequency of hand hygiene has increased due to the COVID-19 pandemic, but there is little evidence regarding the impact of water exposure and temperature on skin. The aim of this study is to evaluate the effect of water exposure and temperature on skin barrier function in healthy individuals. A prospective observational study was conducted. Temperature, pH, transepidermal water loss (TEWL), erythema and stratum corneum hydration (SCH) were measured objectively before and after hot- and cold-water exposure and TempTest® (Microcaya TempTest, Bilbao, Spain) contact. Fifty healthy volunteers were enrolled. Hot-water exposure increased TEWL (25.75 vs. 58.58 g·h−1·m−2), pH (6.33 vs. 6.65) and erythema (249.45 vs. 286.34 AU). Cold-water immersion increased TEWL (25.75 vs. 34.96 g·h−1·m−2) and pH (6.33 vs. 6.62). TEWL (7.99 vs. 9.98 g·h−1·m−2) and erythema (209.07 vs. 227.79 AU) increased after being in contact with the hot region (44 °C) of the TempTest. No significant differences were found after contact with the cold region (4 °C) of the TempTest. In conclusion, long and continuous water exposure damages skin barrier function, with hot water being even more harmful. It would be advisable to use cold or lukewarm water for handwashing and avoid hot water. Knowing the proper temperature for hand washing might be an important measure to prevent flares in patients with previous inflammatory skin diseases on their hands.

The Role of Clindamycin Phosphate Associated with Adapalene in Three Semisolid Formulations Developed for Topical Acne Treatment

2017 ◽  
Vol 68 (5) ◽  
pp. 937-943
Author(s):  
Stela Mariana Al Hussein ◽  
Nicoleta Todoran ◽  
Silvia Imre ◽  
Hussam Al Hussein ◽  
Ana Melero Zaera ◽  
...  
Keyword(s):  
Barrier Function ◽  
Antimicrobial Agents ◽  
Acne Vulgaris ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Clindamycin Phosphate ◽  
Oil In Water ◽  
Study Results

Despite the fact that in mild-to moderate acne vulgaris the standard first-line therapy is the topical treatment with fixed combinations of antimicrobial agents and retinoids, the skin type and the skin barrier function should be taken into account when formulating a topical product. The aim of this study was the comparison of three new semisolid formulations developed for topical application by evaluation of their rheological behavior, as well as the evaluation of in vitro percutaneous diffusion through human epidermis membrane of the pharmaceutical ingredients. Clindamycin phosphate and adapalene were incorporated in three different topical bases, an HPLC method for the determination of their content in the new formulations being developed and validated. A higher concentration of drugs was released from the two gel systems (hydroxypropylmethylcellulose 2.5% -F1 and hydroxyethylcellulose 3% -F2) than from the oil-in-water cream (F3) at pH 7.4, whereas at pH 5.5 the drugs were released in higher amounts from the formulation F3. Following the rheological behavoir associated with the penetrability through the human epidermis membrane, our study results suggest that F1 and F2 could be appropriate in treating acne lesions in patients with oily skin and unaffected skin barrier function. In contrast, the oil-in-water cream (F3), due to its possible emolient effect and its higher penetrability at pH 5.5 than gel vehicles, may be indicated for patients with dry and sensitive skin associated with an altered skin barrier.

scholarly journals Schizandrin A Alleviates LPS-Induced Injury in Human Keratinocyte Cell Hacat Through a MicroRNA-127-Dependent Regulation

2018 ◽  
Vol 49 (6) ◽  
pp. 2229-2239 ◽  
Author(s):  
Shujie Li ◽  
Ruijin Xie ◽  
Chengrui Jiang ◽  
Mei Liu
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Skin Diseases ◽  
Cell Counting ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hacat Cells ◽  
Protective Effects ◽  
Human Keratinocyte ◽  
Tnf Α ◽  
Keratinocyte Cell

Background/Aims: Inflammatory skin diseases are the most common problems in dermatology. Schizandrin A (SchA) has been reported to have anti-inflammatory properties. Herein, we aimed to investigate the protective effects of SchA on lipopolysaccharide (LPS)-induced injury in keratinocyte HaCaT cells. Methods: Inflammation injury in HaCaT cells was induced by LPS treatment. Cell viability, apoptotic cell rate, and apoptosis-related proteins were analyzed by cell counting kit-8 (CCK-8) assay, Annexin V-(fluorescein isothiocyanate (FITC)/ Propidium Iodide (PI) double staining method, and western blot, respectively. The pro-inflammatory factors were analyzed by western blot and quantified by enzyme linked immunosorbent assay (ELISA). Expression of miR-127 in SchA-treated cells was analyzed by qRT-PCR. The effects of SchA on activations of p38MAPK/ERK and JNK pathways were analyzed by western blot. Results: SchA protected HaCaT cells from LPS-induced inflammation damage via promoting cell viability, suppressing apoptosis. Meanwhile, SchA inhibited IL-1β, IL-6, and TNF-α expression. miR-127 expression was up-regulated in LPS-treated HaCaT cells but down-regulated after SchA treatment. Overexpression of miR-127 inhibited cell growth and induced expression of IL-1β, IL-6 and TNF-α. Additionally, miR-127 overexpression impaired the protective effects of SchA, implying miR-127 might be correlated to the anti-inflammation property of SchA and also involved in inactivation of p38MAPK/ERK and JNK pathways by SchA. Conclusion: miR-127 is involved in the protective functions of SchA on LPS-induced inflammation injury in human keratinocyte cell HaCaT, which might inactivates of p38MAPK/ERK and JNK signaling pathways in HaCaT cells.

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