scholarly journals Comparative Transcriptome Analysis of Two Contrasting Kiwifruit (Actinidia) Genotypes under Waterlogging Stress

2020 ◽  
Author(s):  
Zhi Li ◽  
Yunpeng Zhong ◽  
Danfeng Bai ◽  
Muhammad Abid ◽  
Yongjie Zhang ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Ethylene Biosynthesis ◽  
Differential Regulation ◽  
Comparative Transcriptome ◽  
Waterlogging Stress ◽  
Oxygen Scavenging ◽  
Expression Of Genes ◽  
Manganese Superoxide ◽  
Genes Encoding ◽  
Manganese Superoxide Dismutases

Kiwifruit vines are generally sensitive to waterlogging stress. So far, molecular responses of different kiwifruit genotypes for waterlogging stress are less well-explored. In this study, using RNA-sequencing, we examined transcriptional regulation in the roots of a waterlogging-tolerant genotype KR5 (Actinidia valvata), and a sensitive genotype ‘Hayward’ (Actinidia deliciosa) subjected to 0, 12, 24, and 72 h of waterlogging. Compared with 0 h, transcriptional adjustments of these two genotypes occurred as early as 12 h and became notably pronounced 72 h after waterlogging. Waterlogging stress for 72 h promoted the expression of genes involved in ethylene biosynthesis, sucrose and hexose transport, anaerobic fermentation, nitrate reduction, alanine accumulation, and reactive oxygen scavenging in both genotypes. The differential regulation of genes encoding 9-cis-epoxycarotenoid dioxygenase, phosphoglucomutase, alanine-glyoxylate transaminase, and other enzymes pointed to their diverse strategies upon waterlogging in these two genotypes. In addition, more sucrose and trehalose contents, as well as a higher activity of alcohol dehydrogenase and manganese superoxide dismutases were stimulated in KR5 roots after 72h of waterlogging than that in ‘Hayward’. Overall, our results provided more insights into the molecular basis of the waterlogging response in kiwifruit.

scholarly journals Petal abscission in fragrant roses is associated with large scale differential regulation of the abscission zone transcriptome

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Priya Singh ◽  
Neeraj Bharti ◽  
Amar Pal Singh ◽  
Siddharth Kaushal Tripathi ◽  
Saurabh Prakash Pandey ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Large Scale ◽  
Scale Up ◽  
Differential Regulation ◽  
Regulatory Proteins ◽  
Comparative Transcriptome ◽  
Ethylene Sensitivity ◽  
Core Set ◽  
Genes Encoding ◽  
Rosa Bourboniana

Abstract Flowers of fragrant roses such as Rosa bourboniana are ethylene-sensitive and undergo rapid petal abscission while hybrid roses show reduced ethylene sensitivity and delayed abscission. To understand the molecular mechanism underlying these differences, a comparative transcriptome of petal abscission zones (AZ) of 0 h and 8 h ethylene-treated flowers from R. bourboniana was performed. Differential regulation of 3700 genes (1518 up, 2182 down) representing 8.5% of the AZ transcriptome was observed between 0 and 8 h ethylene-treated R. bourboniana petal AZ. Abscission was associated with large scale up-regulation of the ethylene pathway but prominent suppression of the JA, auxin and light-regulated pathways. Regulatory genes encoding kinases/phosphatases/F-box proteins and transcription factors formed the major group undergoing differential regulation besides genes for transporters, wall modification, defense and phenylpropanoid pathways. Further comparisons with ethylene-treated petals of R. bourboniana and 8 h ethylene-treated AZ (R. hybrida) identified a core set of 255 genes uniquely regulated by ethylene in R. bourboniana AZ. Almost 23% of these encoded regulatory proteins largely conserved with Arabidopsis AZ components. Most of these were up-regulated while an entire set of photosystem genes was prominently down-regulated. The studies provide important information on regulation of petal abscission in roses.

scholarly journals Systemic Expression of Genes Involved in the Plant Defense Response Induced by Wounding in Senna tora

2021 ◽  
Vol 22 (18) ◽  
pp. 10073
Author(s):  
Ji-Nam Kang ◽  
Woo-Haeng Lee ◽  
So Youn Won ◽  
Saemin Chang ◽  
Jong-Pil Hong ◽  
...  
Keyword(s):  
Plant Defense ◽  
Defense Response ◽  
Cysteine Proteinase ◽  
Pathogenic Fungi ◽  
Defense Responses ◽  
Ethylene Biosynthesis ◽  
Cysteine Proteinase Inhibitor ◽  
Mechanical Wounding ◽  
Expression Of Genes ◽  
Genes Encoding

Wounds in tissues provide a pathway of entry for pathogenic fungi and bacteria in plants. Plants respond to wounding by regulating the expression of genes involved in their defense mechanisms. To analyze this response, we investigated the defense-related genes induced by wounding in the leaves of Senna tora using RNA sequencing. The genes involved in jasmonate and ethylene biosynthesis were strongly induced by wounding, as were a large number of genes encoding transcription factors such as ERFs, WRKYs, MYBs, bHLHs, and NACs. Wounding induced the expression of genes encoding pathogenesis-related (PR) proteins, such as PR-1, chitinase, thaumatin-like protein, cysteine proteinase inhibitor, PR-10, and plant defensin. Furthermore, wounding led to the induction of genes involved in flavonoid biosynthesis and the accumulation of kaempferol and quercetin in S. tora leaves. All these genes were expressed systemically in leaves distant from the wound site. These results demonstrate that mechanical wounding can lead to a systemic defense response in the Caesalpinioideae, a subfamily of the Leguminosae. In addition, a co-expression analysis of genes induced by wounding provides important information about the interactions between genes involved in plant defense responses.

Sign in / Sign up

Export Citation Format

Share Document