scholarly journals PERFORMANCE OF PACIFIC WHITE SHRIMP FED PROBIOTICS Lactobacillus plantarum AND Bacillus spp. IN A BIOFLOC SYSTEM

2021 ◽  
Vol 47 ◽  
pp. e652
Author(s):  
Norha Constanza BOLÍVAR-RAMÍREZ ◽  
Ana Clara Chede Pereira SILVA ◽  
Gabriela Soltes FERREIRA ◽  
Walter Quadros SEIFFERT ◽  
Felipe do Nascimento VIEIRA
Keyword(s):  
Water Quality ◽  
Growth Performance ◽  
Digestive Tract ◽  
Lactobacillus Plantarum ◽  
Intestinal Tract ◽  
Heterotrophic Bacteria ◽  
Pacific White Shrimp ◽  
Control Group ◽  
Bacillus Spp ◽  
Vibrio Spp

The present study evaluated the use of the indigenous probiotic Lactobacillus plantarum and commercial probiotic containing Bacillus spp. in the culture of L. vannamei in a biofloc system. Shrimp were fed with four diets: L. plantarum, Bacillus spp., L. plantarum + Bacillus spp. and feed with no additives. Growth performance, water quality variables and microbiological counting of water and digestive tract of shrimp were determined. The control group and L. plantarum treatment showed better growth performance. The highest feed conversion ratio (FCR) and the lowest survival were obtained in the L. plantarum + Bacillus spp. treatment, which had significantly higher nitrite values. Vibrio spp. counts in the water were lower in the L. plantarum and L. plantarum + Bacillus spp. treatments and were lower in the intestinal tract in the L. plantarum treatment. Lactic acid bacteria (LAB) was higher in the L. plantarum treatment in the water and digestive tract. The count of total heterotrophic bacteria (THB) deferred only among Bacillus spp. and L. plantarum + Bacillus spp. treatment, being higher in the latter group. In Bacillus spp. treatment, no presence of LAB was detected in the water or intestinal tract. We conclude that the use of L. plantarum combined with Bacillus spp. negatively affected survival, FCR and water quality, but that the use of L. plantarum alone reduced the presence of Vibrio spp., even though it did not change the growth performance of L. vannamei.

scholarly journals Lactic acid bacteria used as preservative in fresh feed for marine shrimp maturation

2016 ◽  
Vol 51 (11) ◽  
pp. 1799-1805 ◽  
Author(s):  
Genyess Ribeiro Arthur da Silva Vieira ◽  
◽  
Mariana Soares ◽  
Norha Constanza Bolívar Ramírez ◽  
Delano Dias Schleder ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Heterotrophic Bacteria ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Bacterial Microbiota ◽  
Marine Shrimp ◽  
Microbiological Analyses ◽  
Vibrio Spp

Abstract The objective of this work was to evaluate the effect of Lactobacillus plantarum on the preservation of fresh mussels and its effect on the attractiveness, consumption, and midgut bacterial microbiota of Pacific white shrimp broodstock. The experiment evaluated mussels stored with L. plantarum at 4ºC. The controls were: mussels stored at -18ºC without L. plantarum; mussels stored at -18ºC with L. plantarum; and mussels stored at 4ºC without L. plantarum. Microbiological analyses on mussels were performed on days 1, 7, 15, 30, 45, and 60 after processing. Additionally, mussels preserved with L. plantarum and stored at 4ºC, and mussels stored at -18ºC without L. plantarum were evaluated after 15 days for attractiveness, consumption, and midgut bacterial microbiota of shrimps. Mussels preserved with L. plantarum showed higher lactic acid bacteria counts and lower counts of Vibrio spp., as well as of total heterotrophic bacteria, after 60 days of storage. No differences were observed for attractiveness or consumption between treatments. The bacterial microbiota of midgut in shrimp fed mussels preserved with L. plantarum showed higher lactic acid bacteria count and lower Vibrio spp. The use of L. plantarum inhibits Vibrio spp. and preserves feed without changing attractiveness or consumption for shrimp.

scholarly journals ẢNH HƯỞNG CỦA MẬT ĐỘ VI KHUẨN Streptomyces DH3.4 LÊN ENZYME TIÊU HÓA VÀ TĂNG TRƯỞNG CỦA TÔM THẺ CHÂN TRẮNG (Litopenaeus vannamei)

2021 ◽  
Vol 5 (3) ◽  
pp. 2710-2719
Author(s):  
Vũ Hùng Hải ◽  
Phạm Thị Tuyết Ngân ◽  
Vũ Ngọc Út ◽  
Huỳnh Trường Giang
Keyword(s):  
Water Quality ◽  
Weight Gain ◽  
Growth Performance ◽  
Digestive Tract ◽  
Litopenaeus Vannamei ◽  
Digestive Enzyme ◽  
Survival Rates ◽  
Total Alkalinity ◽  
Daily Weight Gain ◽  
Control Group

Nghiên cứu được thực hiện nhằm đánh giá ảnh hưởng của việc bổ sung vi khuẩn Streptomyces DH3.4 lên chất lượng nước, vi khuẩn đường ruột, enzyme tiêu hóa và tăng trưởng của tôm thẻ chân trắng (Litopenaeus vannamei). Tôm thẻ chân trắng với trọng lượng trung bình 0,48 ± 0,01 g được chọn để bố trí thí nghiệm với 4 nghiệm thức, mỗi nghiệm thức với 3 lần lặp lại bao gồm (i) Tôm được cho ăn thức ăn không bổ sung khuẩn (Đối chứng) và thức ăn có bổ sung vi khuẩn Streptomyces DH3.4 tương ứng với 3 mật độ (ii) 106, (iii) 107 và (iv) 108 CFU/kg trong 63 ngày. Kết quả cho thấy các thông số chất lượng nước bao gồm nhiệt độ, pH, DO, COD, TSS, độ kiềm, TAN và N-NO2- ở các nghiệm thức bổ sung khuẩn và đối chứng không có sự chênh lệch đáng kể. Mật độ vi khuẩn Streptomyces sp. trong ruột tôm ở nhóm bổ sung vi khuẩn cao hơn có ý nghĩa so với đối chứng. Nghiệm thức bổ sung vi khuẩn Streptomyces DH3.4 ở mật độ 107 và 108 CFU/kg giúp giảm đáng kể mật độ tổng khuẩn Vibrio sp. trong ruột tôm. Các enzyme tiêu hóa như α-amylase, β-galactosidase, protease và Leu-aminopeptidase ở nhóm bổ sung vi khuẩn Streptomyces DH3.4 đạt hoạt tính cao nhất ở cả hai nhóm bổ sung mật độ 107 và 108 CFU/kg.  Tăng trưởng về khối lượng (WG), tốc độ tăng trưởng tương đối (DWG) và tốc độ tăng trưởng tuyệt đối (SRG) về khối lượng khác biệt không đáng kể giữa các nghiệm thức. Tuy nhiên, tỉ lệ sống, sinh khối của tôm và chỉ số FCR ở các nghiệm thức bổ sung Streptomyces DH3.4 được cải thiện đáng kể, đặc biệt là nghiệm thức bổ sung vi khuẩn Streptomyces DH3.4 ở mật độ 107 CFU/kg. ABSTRACT The present study aimed to evaluate the effect of dietary supplementation of Streptomyces DH3.4 on water quality, intestinal bacteria, digestive enzyme activities and growth performance of whiteleg shrimp (Litopenaeus vannamei). Shrimp with initial weight of 0.48 ± 0.01g were designed in 4 treatments with 3 replicates including (i) Shrimp fed a basic diet without bacteria supplementation (control group) and 3 Streptomyces DH3.4 supplementation diets at dose of (ii) 106, (iii) 107 and (iv) 108 CFU/kg for 63 days. The results showed that the water quality parameters including temperature, pH, DO, COD, TSS, total alkalinity, TAN and N-NO2- were not significantly different among treatments. Streptomyces counts in the digestive tract of shrimp fed diets containing Streptomyces DH3.4 were significantly higher than the control. Furthermore, supplementation of 107 and 108 CFU/kg decreased considerably total Vibrio counts in the shrimp’s digestive tract. The digestive enzymes such as α-amylase, β-galactosidase, protease and Leu-aminopeptidase were improved and reached the highest activities in Streptomyces DH3.4 supplementation diet treatments of 107 and 108 CFU/kg. Growth performance parameters regarding weight gain (WG), daily weight gain (DWG) and specific growth rate (SRG) were not significantly different among treatments. However, survival rates, shrimp biomass and FCR in diets containing Streptomyces DH3.4 were improved significantly, especially in 107 CFU/kg treatment.

scholarly journals 362 Effect of a modified hydrated sodium calcium aluminosilicate adsorbent on the growth performance, nutrients digestibility, and digestive tract morphology of chicks challenged with T-2 toxin

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 129-130
Author(s):  
Kun-Tan Wu ◽  
Lv-hui Sun ◽  
Jin-Tao Wei ◽  
Ni-Ya Zhang
Keyword(s):  
Growth Performance ◽  
Digestive Tract ◽  
Body Weight Gain ◽  
Development Program ◽  
Dietary Supplementation ◽  
Lymphoid Cells ◽  
Control Group ◽  
Sodium Calcium ◽  
Calcium Aluminosilicate ◽  
Nutrients Digestibility

Abstract The objective of this study was to evaluate the modified hydrated sodium calcium aluminosilicate (HSCAS) adsorbent ability to reduce the toxicity of T-2 toxin in broilers. 96 one-day-old male broilers were randomly allocated to 4 experimental groups with 4 replicates of 6 birds each. The four groups 1–4 were received the basal diet (BD), BD plus 6.0 mg/kg T-2 toxin, BD plus 6.0 mg/kg T-2 toxin with 0.05% modified HSCAS adsorbent, BD plus 0.05% modified HSCAS adsorbent, respectively, for 2 weeks. The growth performance, nutrients digestibility, and digestive tract histopathology were analyzed. Compared with the control group, dietary supplementation of T-2 toxin decreased (P < 0.05) body weight gain, feed intake and feed conversion by 11.4–31.8% during d 1–7, d 8–14 and d 1–14. Dietary supplementation of T-2 toxin also decreased (P < 0.05) the apparent metabolic rate of crude protein, calcium, and total phosphorus by 14.9–16.1% during d 8–14. These alterations induced by T-2 toxin were mitigated or prevented (P < 0.05) by the supplementation of the modified HSCAS adsorbent. Meanwhile, dietary modified HSCAS adsorbent supplementation also prevented (P < 0.05) T-2 toxin-induced morphological changes and damage, such as severe degeneration and desquamation of the villous epithelial cells, congestion in intestinal lamina propria, and edema and thicken in the serosa with infiltration of numerous lymphoid cells, in the gizzard, duodenum, jejunum, and ileum of broilers. Notably, dietary supplementation of the modified HSCAS adsorbent alone did not affect (P > 0.05) any of those parameters. In conclusion, these findings indicate this novel HSCAS could be used as a promising adsorbent for protecting against T-2 toxin-induced toxicity in chicks (This work was supported in part by the National Key Research and Development Program of China, Projects 2018YFD0500601 and 2016YFD0501207).

scholarly journals Alternate livelihood development for ‘Aila’ affected tribal people through aquaculture in Bali Island of the Sunderban, West Bengal, India

2017 ◽  
Vol 64 ◽  
Author(s):  
P. P. Chakrabarti ◽  
A. Ghosh ◽  
B. C. Mohapatra ◽  
N. K. Barik ◽  
A. Das ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
C:N Ratio ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Natural Food ◽  
Feed Conversion ◽  
Percentage Mortality ◽  
Cumulative Percentage

The concept of “biofloc technology” is changing the facet of intensive aquaculture with scope to attain high productivity in a sustainable manner. In biofloc, dense heterotrophic bacterial community is developed through C:N ratio manipulation, where the system becomes bacterial dominated rather than algae dominated and takes care of the wastes generated through in situ bioremediation. Protein is utilised in two ways; as feed for the shrimp and as microbial floc when the heterotrophic microbes convert the nitrogenous wastes into protein. It also promises a healthy rearing system, which is increasingly identified as one possible solution for disease problems especially those striking at early stages. The purpose of this study was to evaluate the effect of biofloc and periphyton technology (BPT) on the growth and immunomodulatory performance of Pacific white shrimp Penaeus vannamei during nursery and grow-out culture. The experimental BPT treatments with three tier substrate system with molasses as carbohydrate (CHO) source were compared with the conventional autotrophic system. The immunomodulation and cumulative percentage mortality upon challenge with pathogenic strain of Vibrio parahaemolyticus were assessed in the reared animals. We have successfully demonstrated the BPT based nursery and growout systems for P. vannamei with the advantage of providing significantly (p<0.05) better growth (27.6% improvement in average body weight, ABW) and feed utilisation (31% improvement in feed conversion ratio, FCR). A production level of 4-4.5 kg m-3 of water was achieved through this BPT system registering a significant improvement over the conventional system (p<0.05). The cumulative percentage mortality following pathogen challenge was significantly lower (p<0.05) in the biofloc grown shrimps compared to that of the control group, thus showing better resistance to pathogenic challenge. Furthermore, the biofloc reared shrimp did exhibit significant improvement in non-specific immune response in terms of serum phenoloxidase activity and total haemocyte counts possibly suggesting potential immunostimulatory role of the biofloc associated heterotrophic bacteria. This eco-based technology as revealed through our studies brings substantial improvement in productivity, minimising water requirement, recycling in situ nutrients and organic matter in turn improving farm biosecurity, augmentation of natural food, improvement of FCR and better health of the cultured shrimp.

scholarly journals Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

2008 ◽  
Vol 43 (6) ◽  
pp. 763-769 ◽  
Author(s):  
Felipe do Nascimento Vieira ◽  
Celso Carlos Buglione Neto ◽  
José Luiz Pedreira Mouriño ◽  
Adolfo Jatobá ◽  
Cristina Ramirez ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Lactobacillus Plantarum ◽  
Oxidase Activity ◽  
Control Diet ◽  
Phenol Oxidase ◽  
Control Group ◽  
Phenol Oxidase Activity ◽  
Commercial Diet ◽  
Bacterial Microbiota ◽  
Significant Difference

The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the 4th day of evaluation, the total haemocyte counts in shrimps after challenge with V. harveyi were higher in probiotic-supplemented group than in control group. Significant difference was not observed in phenol oxidase activity. On the 6th day after shifting from supplemented to control diet, all parameters were equal in both groups, suggesting that the time-related action of L. plantarum in shrimp is short.

scholarly journals Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

2010 ◽  
Vol 62 (3) ◽  
pp. 631-638 ◽  
Author(s):  
F.N. Vieira ◽  
C.C. Buglione ◽  
J.P.L. Mouriño ◽  
A. Jatobá ◽  
M.L. Martins ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Vibrio Harveyi ◽  
Control Group ◽  
Immunological Parameters ◽  
Bacterial Microbiota ◽  
Total Hemocyte Count ◽  
Lactic Bacteria ◽  
Vibrio Spp ◽  
Serum Agglutination ◽  
Experimental Challenge

The effect of a Lactobacillus plantarum-supplemented diet on shrimp growth, digestive tract bacterial microbiota, survival, and some hemato-immunological parameters after an experimental challenge with Vibrio harveyi was studied. No difference (P>0.05) was observed in shrimp survival (80±6%) and final weight (6.63±0.56g) after 60 days feeding trial. Total bacteria count and Vibrio spp. count in the digestive tract were not diferent (P>0.05) until day 40, but they were lower (P<0.05) in the probiotic-supplemented group on day 60. Total lactic bacteria in the shrimp digestive tract was higher after day 20 in the probiotic-supplemented group. Ten hours after V. harveyi challenge, survival of the probiotic-supplemented group (65.7±2.9%) was higher (P>0.05) than the control group (39.9±4.4%). Bacterial counts in hemolymph and hepatopancreas was lower (P<0.05) in the probiotic-supplemented group than in the control group after V. harveyi challenge. Total hemocyte count and serum agglutination activity were higher (P>0.05) in the probiotic-supplemented group after challenge with V. harveyi. Probiotic-supplemented diet modifies shrimp digestive tract bacterial microbiota, increasing resistance to V. harveyi infection.

scholarly journals Supplementation of Ex-Situ Biofloc to Improve Growth Performance and Enhance Nutritional Values of the Pacific White Shrimp Rearing at Low Salinity Conditions

2021 ◽  
Vol 11 (10) ◽  
pp. 4598
Author(s):  
Umaporn Uawisetwathana ◽  
Magdalena Lenny Situmorang ◽  
Sopacha Arayamethakorn ◽  
Haniswita ◽  
Gede Suantika ◽  
...  
Keyword(s):  
Climate Change ◽  
Water Quality ◽  
Growth Performance ◽  
Culture System ◽  
Pacific White Shrimp ◽  
Ex Situ ◽  
Intensive Culture ◽  
Shrimp Culture ◽  
Promising Alternative ◽  
Low Salinity

Shrimp is an important food source consumed worldwide. An intensive aquaculture system with overuse of feed in combination with detrimental effects from climate change are serious problems leading to mass mortality of cultured shrimp. Biofloc technology is an approach to managing water quality and controlling the disease to counter the negative side of intensive culture system; however, most of the biofloc applications are naturally formed, which could be inconsistent. In this study, we employed an established optimal ratio of microbial consortium called “ex-situ biofloc (BF)” to be used as a feed supplement in shrimp cultured in a zero-water discharged system at low salinity conditions. Three feeding groups (100%commercial pellet (C), 95%C+BF, 90%C+BF) of shrimp were cultured for six weeks. The effect of an ex-situ biofloc supplement with commercial pellet reduction showed that levels of ammonium, nitrite, nitrate and phosphate were significantly decreased in water culture. Shrimp fed with ex-situ biofloc supplement with commercial pellet reduction exhibited significantly increased shrimp weight and survival, and significantly expressed growth-related genes involving lipolysis and energy metabolism higher than those fed with 100% commercial pellet. Nutritional analysis indicated a significant increase of docosahexaenoic acid (DHA) and eicosenoic acid (C20:1) concentrations in the ex-situ biofloc supplemented shrimp. This finding revealed the potential of ex-situ biofloc to manage water quality, improve shrimp growth performance and enhance shrimp nutritional value under intensive culture at low salinity conditions. The beneficial effects of the ex-situ biofloc in shrimp culture system make it a promising alternative strategy to mitigate climate change effects leading to the sustainable production of high-quality shrimp in the future.

scholarly journals The Effects of Different UVA Photoperiods on the Growth Performance, Immune Responses, Antioxidant Status and Apoptosis-Related Gene Expression of the Pacific White Shrimp (Penaeus vannamei)

Antibiotics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 37
Author(s):  
Xinyi Wang ◽  
Baoliang Liu ◽  
Xiaoqiang Gao ◽  
Xi Wang ◽  
Hongxu Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Growth Performance ◽  
Antioxidant Status ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Penaeus Vannamei ◽  
Related Gene ◽  
Apoptosis Related Gene

UVA is the most common type of solar UV radiation in aquatic environments; however, the effects it causes in shrimp farming in recirculating water systems (RAS) is unclear. Thus, the growth performance, immune responses, antioxidant status and apoptosis-related gene expression in Pacific white shrimp, Penaeus vannamei (body weight 9.56 ± 0.10 g), reared with 12L: 12D full spectrum light as background light under five UVA (peak at 400 nm) photoperiods (0L: 24D, 2L: 22D, 4L: 20D, 8L: 16D and 12L: 12D) at a light intensity of 1 W/m2 were investigated. The results showed that the 2L: 22D and 4L: 20D UVA photoperiods enhanced the growth performance and reduced the feed conversion ratio and the shrimp mortality. Shrimp exposed to UVA (2L: 22D and 4L: 20D) also displayed higher levels of hepatopancreas catalase (CAT), superoxide dismutase (SOD), acid phosphatase (ACP), phenol oxidase (PO) and lysozyme (LZM) compared to the 8L: 16D and 12L: 12D groups. The malondialdehyde (MDA) levels increased in line with the extension of the UVA irradiation time. The mRNA expression of apoptosis-related genes in all the UVA treatments were significantly higher than with the control treatment, except for the 2L: 22D group. The results of the 2L: 22D and 4L: 20D treatments were significantly higher than those of the control group, except for LGBP. In conclusion, 2L: 22D and 4L: 20D UVA photoperiods increased growth performance and decreased FCR, improved the innate immunity and antioxidant response and reduced the mortality rate in adult shrimp.

scholarly journals Biofloc based technology evaluation for nutrient dense culture system for nursery and grow-out farming of Pacific white shrimp Penaeus vannamei Boone, 1931

2017 ◽  
Vol 64 ◽  
Author(s):  
A. Panigrahi ◽  
M. Sundaram ◽  
J. Jebha ◽  
J. Syamadayal ◽  
S. K. Otta ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
C:N Ratio ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Natural Food ◽  
Penaeus Vannamei ◽  
Percentage Mortality ◽  
Cumulative Percentage

The concept of “biofloc technology” is changing the facet of intensive aquaculture with scope to attain high productivity in a sustainable manner. In biofloc, dense heterotrophic bacterial community is developed through C:N ratio manipulation, where the system becomes bacterial dominated rather than algae dominated and takes care of the wastes generated through in situ bioremediation. Protein is utilised in two ways; as feed for the shrimp and as microbial floc when the heterotrophic microbes convert the nitrogenous wastes into protein. It also promises a healthy rearing system, which is increasingly identified as one possible solution for disease problems especially those striking at early stages. The purpose of this study was to evaluate the effect of biofloc and periphyton technology (BPT) on the growth and immunomodulatory performance of Pacific white shrimp Penaeus vannamei during nursery and grow-out culture. The experimental BPT treatments with three tier substrate system with molasses as carbohydrate (CHO) source were compared with the conventional autotrophic system. Theimmunomodulation and cumulative percentage mortality upon challenge with pathogenic strain of Vibrio parahaemolyticus were assessed in the reared animals. We have successfully demonstrated the BPT based nursery and grow-out systems for P. vannamei with the advantage of providing significantly (p<0.05) better growth performance (27.6% improvement inaverage body weight, ABW) and feed utilisation (31% improvement in feed conversion ratio, FCR). A production level of 4-4.5 kg m-3 of water was achieved through this BPT system registering a significant improvement over the conventional system (p<0.05). The Cumulative percentage mortality following pathogen challenge was significantly lower (p<0.05) in the biofloc grown shrimps compared to that of the control group, thus showing better resistance to pathogenic challenge. Furthermore, the biofloc reared shrimp did exhibit significant improvement in non-specific immune response in terms of serum phenoloxidase activity and total haemocyte counts possibly suggesting potential immunostimulatory role of the biofloc associated heterotrophic bacteria. This eco-based technology as revealed through our studies brings substantial improvement in productivity, minimising water requirement, recycling in situ nutrients and organic matter in turn improvingfarm biosecurity, augmentation of natural food, improvement of FCR and better health of the cultured shrimp.

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