scholarly journals ẢNH HƯỞNG CỦA MẬT ĐỘ VI KHUẨN Streptomyces DH3.4 LÊN ENZYME TIÊU HÓA VÀ TĂNG TRƯỞNG CỦA TÔM THẺ CHÂN TRẮNG (Litopenaeus vannamei)

2021 ◽  
Vol 5 (3) ◽  
pp. 2710-2719
Author(s):  
Vũ Hùng Hải ◽  
Phạm Thị Tuyết Ngân ◽  
Vũ Ngọc Út ◽  
Huỳnh Trường Giang
Keyword(s):  
Water Quality ◽  
Weight Gain ◽  
Growth Performance ◽  
Digestive Tract ◽  
Litopenaeus Vannamei ◽  
Digestive Enzyme ◽  
Survival Rates ◽  
Total Alkalinity ◽  
Daily Weight Gain ◽  
Control Group

Nghiên cứu được thực hiện nhằm đánh giá ảnh hưởng của việc bổ sung vi khuẩn Streptomyces DH3.4 lên chất lượng nước, vi khuẩn đường ruột, enzyme tiêu hóa và tăng trưởng của tôm thẻ chân trắng (Litopenaeus vannamei). Tôm thẻ chân trắng với trọng lượng trung bình 0,48 ± 0,01 g được chọn để bố trí thí nghiệm với 4 nghiệm thức, mỗi nghiệm thức với 3 lần lặp lại bao gồm (i) Tôm được cho ăn thức ăn không bổ sung khuẩn (Đối chứng) và thức ăn có bổ sung vi khuẩn Streptomyces DH3.4 tương ứng với 3 mật độ (ii) 106, (iii) 107 và (iv) 108 CFU/kg trong 63 ngày. Kết quả cho thấy các thông số chất lượng nước bao gồm nhiệt độ, pH, DO, COD, TSS, độ kiềm, TAN và N-NO2- ở các nghiệm thức bổ sung khuẩn và đối chứng không có sự chênh lệch đáng kể. Mật độ vi khuẩn Streptomyces sp. trong ruột tôm ở nhóm bổ sung vi khuẩn cao hơn có ý nghĩa so với đối chứng. Nghiệm thức bổ sung vi khuẩn Streptomyces DH3.4 ở mật độ 107 và 108 CFU/kg giúp giảm đáng kể mật độ tổng khuẩn Vibrio sp. trong ruột tôm. Các enzyme tiêu hóa như α-amylase, β-galactosidase, protease và Leu-aminopeptidase ở nhóm bổ sung vi khuẩn Streptomyces DH3.4 đạt hoạt tính cao nhất ở cả hai nhóm bổ sung mật độ 107 và 108 CFU/kg.  Tăng trưởng về khối lượng (WG), tốc độ tăng trưởng tương đối (DWG) và tốc độ tăng trưởng tuyệt đối (SRG) về khối lượng khác biệt không đáng kể giữa các nghiệm thức. Tuy nhiên, tỉ lệ sống, sinh khối của tôm và chỉ số FCR ở các nghiệm thức bổ sung Streptomyces DH3.4 được cải thiện đáng kể, đặc biệt là nghiệm thức bổ sung vi khuẩn Streptomyces DH3.4 ở mật độ 107 CFU/kg. ABSTRACT The present study aimed to evaluate the effect of dietary supplementation of Streptomyces DH3.4 on water quality, intestinal bacteria, digestive enzyme activities and growth performance of whiteleg shrimp (Litopenaeus vannamei). Shrimp with initial weight of 0.48 ± 0.01g were designed in 4 treatments with 3 replicates including (i) Shrimp fed a basic diet without bacteria supplementation (control group) and 3 Streptomyces DH3.4 supplementation diets at dose of (ii) 106, (iii) 107 and (iv) 108 CFU/kg for 63 days. The results showed that the water quality parameters including temperature, pH, DO, COD, TSS, total alkalinity, TAN and N-NO2- were not significantly different among treatments. Streptomyces counts in the digestive tract of shrimp fed diets containing Streptomyces DH3.4 were significantly higher than the control. Furthermore, supplementation of 107 and 108 CFU/kg decreased considerably total Vibrio counts in the shrimp’s digestive tract. The digestive enzymes such as α-amylase, β-galactosidase, protease and Leu-aminopeptidase were improved and reached the highest activities in Streptomyces DH3.4 supplementation diet treatments of 107 and 108 CFU/kg. Growth performance parameters regarding weight gain (WG), daily weight gain (DWG) and specific growth rate (SRG) were not significantly different among treatments. However, survival rates, shrimp biomass and FCR in diets containing Streptomyces DH3.4 were improved significantly, especially in 107 CFU/kg treatment.

Effects of dietary selenium-yeast concentrations on growth performance and carcass composition of ducks

2017 ◽  
Vol 57 (8) ◽  
pp. 1731 ◽  
Author(s):  
Milan Ž. Baltić ◽  
Marija Dokmanović Starčević ◽  
Meho Bašić ◽  
Amir Zenunović ◽  
Jelena Ivanović ◽  
...  
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Subcutaneous Fat ◽  
Conversion Ratio ◽  
Carcass Composition ◽  
Feed Conversion Ratio ◽  
Daily Weight Gain ◽  
Control Group ◽  
Feed Conversion ◽  
Dressing Percentage

The aim of the present study was to assess effects of selenium (Se)-yeast diet supplementation on performance and carcass composition in ducks. The study was performed on 240 1-day old ducklings of the same origin (Cherry Valley hybrid), during a 49-day period, which were fed diets supplemented with the following four different levels of Se yeast (ALKOSEL® R397): groups with Se at 0 mg/kg of the diet as-fed, 0.2 mg/kg, 0.4 mg/kg and 0.6 mg/kg. Growth performance (bodyweight, daily weight gain, daily feed intake, feed-conversion ratio) and carcass characteristics (hot and cold carcass weight, chilling losses, dressing percentage, carcass cut yields, and percentage of tissues in breast and thighs with drumsticks) of the ducks were determined. Animals fed high-Se diets (0.4 mg/kg) had higher (P < 0.05) final bodyweight and daily weight gain (from 15 to 49 days) compared with those fed diets with inadequate (0 mg/kg) or with supranutritional (0.6 mg/kg) Se levels. Ducks fed only with basal diet showed a higher (P < 0.05) feed-conversion ratio (from 15 to 49 days) compared with those supplemented with Se at 0.4 mg/kg and 0.6 mg/kg. Dressing percentage was higher (P < 0.01) in the control group (69.50%) than in the group with Se added at 0.6 mg/kg (66.85%). The weights of basic cuts from the duck carcasses did not significantly differ among compared groups. Moreover, the percentage of skin with subcutaneous fat in breast and thighs with drumsticks increased in Se-supplemented groups compared with the control group, while the opposite was determined with the percentage of muscle. It was concluded that a diet containing 0.4 mg of added Se per kilogram produced the greatest growth-performance results in ducks.

scholarly journals Spirulina (Arthrospira fusiformis) as a potential protein source in practical diets for fry mariculture of Rufiji tilapia (Oreochromis urolepis urolepis)

2019 ◽  
Vol 18 (2) ◽  
pp. 57-67
Author(s):  
Deogratias P. Mulokozi ◽  
Matern S. Mtolera ◽  
Aviti J. Mmochi
Keyword(s):  
Growth Performance ◽  
Culture Media ◽  
Salt Water ◽  
Control Diet ◽  
Survival Rates ◽  
Daily Weight Gain ◽  
Feed Additive ◽  
Control Fish ◽  
Control Group ◽  
Feed Conversion

The effects on growth performance, feed utilization, survival and whole fish body proximate composition of replacing fishmeal (Rastrineobola argentea) with a locally available spirulina species (Arthrospira fusiformis) as a protein ingredient in the feed of Rufiji tilapia (Oreochromis urolepis urolepis) fry was examined. Fishmeal replacement with A. fusiformis was carried out at 5%, 15%, 25%, 35% and 100% (S5, S15, S25, S35 and S100), and the effect of the replacement was compared with the control diet (S0, 0% spirulina). Fish fry were stocked at an average initial weight of 0.57 g at 10 fish per 0.1 m3 and cultured for 60 days using full strength salt water (30-35 ppt). A. fusiformis was isolated from Momela Lake in Arusha National Park, Tanzania and propagated using selected culture media. It was observed that fish fry fed spirulina at a 5% fishmeal substitution level diet had better (p<0.05) final weight (8.48), average daily weight gain (0.132), specific growth rate (4.47), feed conversion rate (2.08) and protein efficiency ratio (1.37) compared to the control fish group. Growth performance in fish fed diet S15 was comparable with the control group (p>0.05). Spirulina supplementation had no effect on fish survival rates and meat quality. It appears that the Momella Lake spirulina may be an appropriate growth-stimulating plant protein when used as a feed additive in Rufiji tilapia mariculture. 

scholarly journals Effects of carbon to nitrogen ratio (C:N) on water quality and growth performance of Litopenaeus vannamei (Boone, 1931) in the biofloc system with a salinity of 5%

2021 ◽  
Author(s):  
Hai-Hong Huang
Keyword(s):  
Water Quality ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Survival Rates ◽  
The Other ◽  
Culture Experiment ◽  
Carbon To Nitrogen Ratio ◽  
Low Salinity ◽  
Salinity Condition ◽  
Nitrogen Ratio

This study aimed to investigate the effects of carbon to nitrogen ratio (C:N) on the water quality and shrimp growth performance during the grow-out culture of Litopenaeus vannamei in the biofloc system under a low salinity condition. Three biofloc treatments with an C:N (contained in the inputted feed and carbon source with the assumption that 75% of the feed nitrogen is excreted) of 8:1 (CN8), 16:1 (CN16) and 24:1 (CN24), respectively, were designed to stocking shrimp juveniles (≈ 0.8 g) at a density of 270 individuals m-3, for a 63-days culture experiment at a salinity of about 5‰. Results showed that in CN8 treatment, the levels of pH (6.9±0.1), carbonate alkalinity (104.0±2.8mg L-1 CaCO3), biofloc volume (4.8±0.9mL L-1) and TSS (327.4±24.4mg L-1) were significantly lower than those in the other two treatments (≥7.6±0.3, ≥157.6±21.6mg L-1 CaCO3, ≥24.1±3.7mL L-1 and ≥508.1±32.3mg L-1, P<0.05); whereas the levels of TAN (7.1±0.9mg L-1), nitrite (14.0±3.6mg L-1) and nitrate (77.0±5.0mg L-1) were significantly higher than those in the other treatments (≤2.0±0.6mg L-1, ≤4.9±3.1mg L-1 and ≤14.7±5.9mg L-1, P<0.05). The zootechnical parameters of shrimp were not significantly different between three treatments (P>0.05), except that the survival rates in CN16 treatment (96.8±2.0%) and CN24 treatment (93.7±4.2%) were significantly higher than that of CN8 treatment (81.5±6.4%, P<0.05). The results indicated that an inputted C:N higher than 16:1 was suitable for the biofloc system with a low salinity of 5‰, with an optimal inferred C:N range of 18.5-21.0:1 for water quality and growth performance.

scholarly journals The Effect of Socialising Piglets During Lactation on Performance, Suckling Behaviour and Weaning Aggression: a Preliminary Field Study

2020 ◽  
Vol 68 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Nikolina Mesarec ◽  
Urška Pačnik ◽  
Alja Mesarič ◽  
Janko Skok ◽  
Dejan Škorjanc ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Daily Weight Gain ◽  
Economic Losses ◽  
Control Group ◽  
Free Access ◽  
Initial Body Weight ◽  
Significant Drop ◽  
Preliminary Study

The post-weaning aggression among piglets is a serious problem in modern pig production which compromises animal welfare and health, as well as could cause considerable economic losses. To prevent post-weaning aggression between piglets an approach of early socialisation of piglets during lactation was tested in the present study. In the group farrowing pen (GFP) three individual farrowing pens were interconnected whereby piglets had free access to move across pens and/or cross-suckled alien sows that were still individually crated – passage between pens was released within 24h postpartum. Piglets/litters in the control group went through the conventional breeding practice (i.e. individual farrowing pen/crate). Altogether, the experiment involved 235 piglets. The preliminary results revealed that the suckling order stability differed between studied breeding technologies (group and individual). About one third of all piglets (31.3%) were found being involved (at least once) in the cross-suckling, i.e. suckling by the alien mother. Consequently, suckling stability was found generally lower in the GFP, with a significant drop after two weeks of lactation. Regarding the growth performance, piglets from GFP grew relatively, i.e. considering initial body weight, faster both during lactation and after weaning, although their daily weight gain was not significantly higher. The present preliminary study revealed early socialisation of piglets as an effective method to mitigate post-weaning aggression, as well as to improve their growth performance. However, considering mortality in lactation and disturbances in suckling stability, additional studies are needed to optimize the process of early socialisation, particularly in terms of piglets’ age when grouping of non-littermates is implemented during lactation.

scholarly journals PERFORMANCE OF PACIFIC WHITE SHRIMP FED PROBIOTICS Lactobacillus plantarum AND Bacillus spp. IN A BIOFLOC SYSTEM

2021 ◽  
Vol 47 ◽  
pp. e652
Author(s):  
Norha Constanza BOLÍVAR-RAMÍREZ ◽  
Ana Clara Chede Pereira SILVA ◽  
Gabriela Soltes FERREIRA ◽  
Walter Quadros SEIFFERT ◽  
Felipe do Nascimento VIEIRA
Keyword(s):  
Water Quality ◽  
Growth Performance ◽  
Digestive Tract ◽  
Lactobacillus Plantarum ◽  
Intestinal Tract ◽  
Heterotrophic Bacteria ◽  
Pacific White Shrimp ◽  
Control Group ◽  
Bacillus Spp ◽  
Vibrio Spp

The present study evaluated the use of the indigenous probiotic Lactobacillus plantarum and commercial probiotic containing Bacillus spp. in the culture of L. vannamei in a biofloc system. Shrimp were fed with four diets: L. plantarum, Bacillus spp., L. plantarum + Bacillus spp. and feed with no additives. Growth performance, water quality variables and microbiological counting of water and digestive tract of shrimp were determined. The control group and L. plantarum treatment showed better growth performance. The highest feed conversion ratio (FCR) and the lowest survival were obtained in the L. plantarum + Bacillus spp. treatment, which had significantly higher nitrite values. Vibrio spp. counts in the water were lower in the L. plantarum and L. plantarum + Bacillus spp. treatments and were lower in the intestinal tract in the L. plantarum treatment. Lactic acid bacteria (LAB) was higher in the L. plantarum treatment in the water and digestive tract. The count of total heterotrophic bacteria (THB) deferred only among Bacillus spp. and L. plantarum + Bacillus spp. treatment, being higher in the latter group. In Bacillus spp. treatment, no presence of LAB was detected in the water or intestinal tract. We conclude that the use of L. plantarum combined with Bacillus spp. negatively affected survival, FCR and water quality, but that the use of L. plantarum alone reduced the presence of Vibrio spp., even though it did not change the growth performance of L. vannamei.

scholarly journals Effects of Bacillus subtilis on growth performance, serum parameters, digestive enzyme, intestinal morphology, and colonic microbiota in piglets

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo Deng ◽  
Jie Wu ◽  
Xiaohui Li ◽  
Cheng Zhang ◽  
Xiaoming Men ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Digestive Enzyme ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Promising Alternative ◽  
Serum Parameters ◽  
Colonic Microbiota ◽  
Diet Supplementation

AbstractThe present study was conducted to investigate effects of Bacillus subtilis on growth performance, serum parameters, digestive enzymes, intestinal morphology, and colonic microbiota in piglets. A total of 72 piglets were weighed and randomly allotted into three treatments (four replication pens per treatment with six piglets/pen) for a 28-day experiment. The dietary treatments were as follows: basal diet (control group, CTR), basal diet supplementation with antibiotic (antibiotic group, ABT), and basal diet supplementation with 0.1% Bacillus subtilis (probiotic group, PBT). The average daily gain of body weight increased in both the ABT and PBT groups, and dietary antibiotics decreased the feed:gain ratio (F:G), as compared to the CTR group (P < 0.05). Both ABT and PBT piglets had increased serum triglycerides and lipase, amylase, maltase activities and villus height:crypt depth ratio (V/C) in ileum (P < 0.05). The PBT group also showed an increase in serum glucose and villus height in the ileum (P < 0.05). Dietary antibiotics increased Lactobacillus johnsonii, as compared to the CTR group, but decreased bacterial diversity and increased Escherichia coli, as compared to the PBT group (P < 0.05). Piglets dietary with B. subtilis modulated the microbiota by increasing the abundance of Firmicutes (L. johnsonii, L. reuteri) and decreasing the abundance of E. coli, as compared to the control group (P < 0.05). These results indicate that dietary of B. subtilis improves growth performance and intestinal health and can be a promising alternative to antibiotics in piglets diet.

Effects of microbubble aeration on water quality and growth performance of Litopenaeus vannamei in biofloc system

2021 ◽  
Vol 93 ◽  
pp. 102159
Author(s):  
Yan Shin Lim ◽  
P. Ganesan ◽  
M. Varman ◽  
F.A. Hamad ◽  
Sivakumar Krishnasamy
Keyword(s):  
Water Quality ◽  
Growth Performance ◽  
Litopenaeus Vannamei

scholarly journals Performance of some growth promoters on hybrid walking catfish (Clarias batrachus × Clarias gariepinus)

2017 ◽  
Vol 3 (1) ◽  
pp. 103-108
Author(s):  
Md Sirazul Islam ◽  
M Mamnur Rashid ◽  
Mohammad Shamsul Arefin ◽  
Pronab Naha ◽  
Md Rokanuddula
Keyword(s):  
Weight Gain ◽  
Control Diet ◽  
Animal Health ◽  
Clarias Gariepinus ◽  
Survival Rates ◽  
Clarias Batrachus ◽  
Daily Weight Gain ◽  
Growth Promoter ◽  
Average Daily Weight Gain ◽  
Growth Promoters

A study was conducted to determine the efficacy of three most commonly used growth promoters from different pharmaceutical companies on growth performance of hybrid walking catfish (Clarias batrachus × Clarias gariepinus). The experiment was done in the wet laboratory of the Department of Aquaculture, BAU, Mymensingh, with a total of 10 aquaria and 160 fish having mean initial weight of 6.3 ± 0.48 g. The three growth promoters, “Charger gel” from Fishtech (BD) Ltd., “Growth gel” from Advance Agrotech (BD) Ltd. and “Hepaprotect-aqua” from Renata Animal health Ltd., were used in separate nine (9) aquaria at recommended, lower dose and higher dose respectively. One aquarium was used as control (diet without any growth promoter). The fish were fed with paragon nursery floating feed at 20% of body weight in each aquarium for 28 days. Doses of Charger gel were given as 80, 60 and 100 mg/20 g feed/day, that of Growth gel, 0.14, 0.12 and 0.16 ml/20 g feed/day and of Hepaprotect-aqua, 30, 15 and 50 mg/20 g feed/day. Re-circulatory system was used throughout the experimental period. By applying recommended, lower and higher dose of “Charger gel”, mean final weights were found as 23.0, 21.9 and 35.9 g; average daily weight gain as 0.60, 0.56 and 1.06 g and specific growth rate (SGR) as 2.01, 1.93 and 2.70% respectively. For “Growth gel”, mean final weights were found as 22.8, 17.3 and 31.1 g; average daily weight gain as 0.59, 0.39 and 0.88 g and SGR as 1.99, 1.57 and 2.48 % respectively. For “Hepaprotect-aqua”, mean final weights were found as 24.2, 17.5 and 28.2 g; average daily weight gain as 0.64, 0.40 and 0.78 g and SGR as 2.09, 1.58 and 2.32% respectively. In all the cases survival rates were 100%. All the three growth promoters showed better results at their higher doses in comparison to their results in lower and recommended doses. However, in average Charger gel showed the best result than the other two growth promoters. This information on the efficacy study of growth promoters needs to be disseminated to the farmers for improved fish production.Asian J. Med. Biol. Res. March 2017, 3(1): 103-108

scholarly journals 362 Effect of a modified hydrated sodium calcium aluminosilicate adsorbent on the growth performance, nutrients digestibility, and digestive tract morphology of chicks challenged with T-2 toxin

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 129-130
Author(s):  
Kun-Tan Wu ◽  
Lv-hui Sun ◽  
Jin-Tao Wei ◽  
Ni-Ya Zhang
Keyword(s):  
Growth Performance ◽  
Digestive Tract ◽  
Body Weight Gain ◽  
Development Program ◽  
Dietary Supplementation ◽  
Lymphoid Cells ◽  
Control Group ◽  
Sodium Calcium ◽  
Calcium Aluminosilicate ◽  
Nutrients Digestibility

Abstract The objective of this study was to evaluate the modified hydrated sodium calcium aluminosilicate (HSCAS) adsorbent ability to reduce the toxicity of T-2 toxin in broilers. 96 one-day-old male broilers were randomly allocated to 4 experimental groups with 4 replicates of 6 birds each. The four groups 1–4 were received the basal diet (BD), BD plus 6.0 mg/kg T-2 toxin, BD plus 6.0 mg/kg T-2 toxin with 0.05% modified HSCAS adsorbent, BD plus 0.05% modified HSCAS adsorbent, respectively, for 2 weeks. The growth performance, nutrients digestibility, and digestive tract histopathology were analyzed. Compared with the control group, dietary supplementation of T-2 toxin decreased (P &lt; 0.05) body weight gain, feed intake and feed conversion by 11.4–31.8% during d 1–7, d 8–14 and d 1–14. Dietary supplementation of T-2 toxin also decreased (P &lt; 0.05) the apparent metabolic rate of crude protein, calcium, and total phosphorus by 14.9–16.1% during d 8–14. These alterations induced by T-2 toxin were mitigated or prevented (P &lt; 0.05) by the supplementation of the modified HSCAS adsorbent. Meanwhile, dietary modified HSCAS adsorbent supplementation also prevented (P &lt; 0.05) T-2 toxin-induced morphological changes and damage, such as severe degeneration and desquamation of the villous epithelial cells, congestion in intestinal lamina propria, and edema and thicken in the serosa with infiltration of numerous lymphoid cells, in the gizzard, duodenum, jejunum, and ileum of broilers. Notably, dietary supplementation of the modified HSCAS adsorbent alone did not affect (P &gt; 0.05) any of those parameters. In conclusion, these findings indicate this novel HSCAS could be used as a promising adsorbent for protecting against T-2 toxin-induced toxicity in chicks (This work was supported in part by the National Key Research and Development Program of China, Projects 2018YFD0500601 and 2016YFD0501207).

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