scholarly journals Tannins Compound In Soga Tingi Bark (Ceriops Tagal) As Natural Dyes

2021 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Paryanto Paryanto ◽  
Sunu Herwi Pranolo ◽  
Ari Diana Susanti ◽  
Bintang Timur Putrikatama ◽  
I. R. Qatrunada ◽  
...  
Keyword(s):  
Textile Industry ◽  
Mobile Phase ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Natural Dyes ◽  
Ftir Analysis ◽  
Tannin Content ◽  
Textile Materials ◽  
Ceriops Tagal ◽  
Guava Leaf

<p>In general, natural dyes for textile materials are obtained from extracts part of the plants such as roots, wood, leaves, seeds, and flower. Textile industry especially batik craftsman, have known many plants that can dye textile materials, such as indigo (<em>indigofera</em><em>)</em>, soga tingi bark (<em>Ceriops tagal</em><em>)</em>, tegeran wood (<em>Cudraina javanensis</em><em>)</em>, turmeric (<em>Curcuma</em>), tea (<em>The</em>), noni root (<em>Morinda citrifelia</em>), soga jambal bark <em>(Pelthophorum ferruginum</em>), kesumba (<em>Bixa orelana</em>), and guava leaf (<em>Psidiumguajava</em>). Soga tingi bark chosen because it can produce tannins which can be used as natural dyes. The purpose of this research was to obtained tannin content in soga tingi bark as qualitatively and quantitatively. The analysis carried out is FTIR and HPLC method. FTIR analysis carried out to determine of the compounds contained in the soga tingi bark extraction. Based on FTIR analysis it can be seen that there are O-H and N-H group in the wavenumber 3375,13 cm<sup>-1</sup>. C=O bond at wavenumber 1739,16 cm<sup>-1</sup>. C=C bond at wavenumber 1624,31 cm<sup>-1</sup>. C-H bond at wavenumbers 2970,72 cm<sup>-1</sup>, 1456,39 cm<sup>-1</sup>, and 1365,74 cm<sup>-1</sup>. NO<sub>2</sub> bond at wavenumber 1365,74 cm<sup>-1</sup>. C-N bond at wavenumbers 1228,69 cm<sup>-1</sup> and 1217,34 cm<sup>-1</sup>. And C-O bond at wavenumbers 1228,69 cm<sup>-1</sup>, 1217,34 cm<sup>-1</sup>, and 1052,3 cm<sup>-1</sup>. While HPLC analysis carried out to determine contains tannin level in the soga tingi bark extraction. HPLC conditions used are Flowrate: 1 mL/min, Mobile phase: MeOH : H<sub>2</sub>O (50:50), λ: 271 nm and Column: C18, 250 mm. Based on HPLC analysis it is known that the contains tannin level in the soga tingi bark extraction is 22,44 ppm.</p>

scholarly journals Validation of Metamizole, Thiamin and Pyridoxine Simultaneous Analysis Methods in Tablet Preparations Using HPLC

2018 ◽  
Vol 1 (1) ◽  
pp. 19
Author(s):  
Vevi Maritha ◽  
Lukman Labasy
Keyword(s):  
Mobile Phase ◽  
Hplc Analysis ◽  
Sodium Sulfite ◽  
Hplc Method ◽  
Optimum Concentration ◽  
Diode Array ◽  
Good Precision ◽  
Chromatographic System ◽  
Chromatographic Condition ◽  
Hydrolysis Of

The aim of the present study was to develop and validate HPLC method for the simultaneous assay of metamizole, thiamine and pyridoxine in tablet. Metamizole is a substance that is easily hydrolyzed in the precence of water and oxygen. To inhibit the hydrolysis of metamizole during sample preparation prior to HPLC analysis, sodium sulfite is added and its optimum concentration was investigated. The chromatographic system includes a RP C8(2) column (150x4.6 mm, 5 µm particle size) in conjunction with Photo Diode Array (PDA) detector. The optimal chromatographic condition was obtained using a mobile phase consisting of phosphate buffer 35mM pH 3.0: methanol (80:20), flowrate 1.0 ml/min, and 10 µl injection volume. The metamizole, thiamine and pyridoxine were detected at 275 nm and 361 nm for cyanocobalamin. The Hydrolysis of metamizole was successfully inhibited by adding solution containing 1.5 mg/mL sodium sulfite to solvent and 0.5 mg/mL sodium sulfite to mobile phase. The validation results indicate a good specificity and a linear detector responses with r>0.999. The accuracy (% recovery) for metamizole, thiamine and piridoxine were 100.26%; 99.09%; and 100.03%, respectively. The method yields good precision with RSD of metamizole, thiamine and pyridoxine were 2.0912%; 1.4489%; and 0.8418% respectively. In the robustness study, the small changes of mobile phase pH yielded unsymmetrical peaks and lower resolution. The validated method was successfully applied for simultaneous assay of metamizole, thiamine and pyridoxine in tablet. Keywords: validation; metamizole; thiamine; pyridoxine; hydrolysis of metamizole; HPLC

scholarly journals STUDI PERBANDINGAN ANALISIS VITAMIN E MINYAK SAWIT MERAH TERSAPONIFIKASI ANTARA METODE SPEKTROFOTOMETRI UV-VIS DAN KCKT

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 50
Author(s):  
Arlina Mayharty Andulaai ◽  
Ruslan Ruslan ◽  
Hardi Ys. ◽  
Dwi Juli Puspitasari
Keyword(s):  
High Performance Liquid Chromatography ◽  
Vitamin E ◽  
Palm Oil ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Ultra Violet ◽  
Hplc Method ◽  
Red Palm Oil ◽  
Extraction Spectrophotometry

A research about a comparative study of spectrophotometry UV-Vis and HPLC method for the analysis of vitamin E in saponified red palm oil has been done. This research aims to compare the results of analysis using Spectrophotometer UV - Vis and HPLC to determine the concentration of vitamin E in red palm oil previously saponified and extracted. HPLC analysis was carried out using an RP-18 column and mobile phase composed a methanol and water ( 86:14 ), with a flow rate of 1 ml/min and UV detection at 290 nm. For the Spectrophotometric UV-Vis analysis, hexane was used as a solvent and the wavelength at 298,5 nm was selected for the detection. The results are the concentration of vitamin E using spectrophotometric and HPLC method was respectively 104.5 ppm and 127 ppm.Keyword: Vitamin E, Red Palm Oil, saponification, extraction, spectrophotometry Ultra Violet -Visible, High Performance Liquid Chromatography

scholarly journals The identification and the quantitative determination of loratadine by the HPLC method

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 40-46
Author(s):  
Olena O. Mamina ◽  
Volodymyr I. Kabachny ◽  
Nataliia Yu. Bondarenko ◽  
Olena V. Lozova
Keyword(s):  
Quantitative Determination ◽  
Mobile Phase ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Therapeutic Monitoring ◽  
Reproducible Research ◽  
Column Temperature ◽  
Model Solutions ◽  
Relative Standard

Aim. To develop the unified method of the HPLC analysis of loratadine, which can allow obtaining reliable and reproducible results of the studies of pharmaceuticals and biological matrices for monitoring the treatment effectiveness.Materials and methods. The HPLC analysis was performed on a “Milichrome A-02” microcolumn liquid chromatograph under the following conditions: a reversed-phase variant, 2 × 75 mm column filled with a non-polar sorbent Prontosil 120-5 C18 AQ, 5 μm; the mobile phase in the mode of a linear gradient – from eluent А (5 % of acetonitrile and 95  % of a buffer solution) to eluent B (100 % of acetonitrile) for 40 min. The flow rate of the mobile phase was 100 μL/min; the injection volume was 4 μL. The multichannel detection of the substance was carried out using an UV-detector at 210, 220, 230, 240, 250, 260, 280 and 300 nm; the optimal value of the column temperature was 37 – 40 °С, and the pump pressure was 2.8 – 3.2 MPa.Results and discussion. As a result of the studies performed, the retention parameters of loratadine and spectral relationships were determined using the unified HPLC method. This made it possible to include the results obtained in the database for the identification of antihistamines in the therapeutic monitoring of the treatment with an individual drug or in the complex treatment of allergic reactions. The development of the quantitative determination of loratadine by HPLC on model solutions using various concentrations of the drug was carried out. The content of loratadine was determined by the equation S = 1.14 × 10-3C – 0.50 × 10-4; the correlation coefficient was 0.9998. It was found that the relative standard deviation RSD did not exceed 0.93 % when analyzing loratadine in the model solutions by HPLC.Conclusions. The identification and the quantitative determination of loratadine by the unified HPLC method have been conducted. The method allows obtaining reliable and reproducible research results. The results of the studies can be recommended for implementation in the practice of forensic bureaus, toxicological centers, and clinical laboratories.

A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Imad Tarek Hanoon ◽  
Abed Mohammed Daheir AL-Joubory 2 ◽  
Marwa Mohamed Saied 3
Keyword(s):  
Mobile Phase ◽  
Chemical Structure ◽  
Quantitative Estimation ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Form ◽  
Rp Hplc ◽  
Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

QUANTITATIVE DETERMINATION OF CURCUMIN IN TURMERIC POWDER AND TURMERIC–HONEY BALL PILLS BY HPLC

2018 ◽  
Vol 8 (4) ◽  
pp. 42-47
Author(s):  
Tien Nguyen Huu ◽  
Tram Le Thi Bao ◽  
Ngoc Nguyen Thi Nhu ◽  
Thang Phan Phuoc ◽  
Khan Nguyen Viet
Keyword(s):  
Acetic Acid ◽  
Gastric Mucosa ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Curcuma Longa ◽  
Biological Marker ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Precision And Accuracy

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC

The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

2018 ◽  
Vol 69 (3) ◽  
pp. 627-631 ◽  
Author(s):  
Viorica Ohriac (Popa) ◽  
Diana Cimpoesu ◽  
Adrian Florin Spac ◽  
Paul Nedelea ◽  
Voichita Lazureanu ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Emotional Experience ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Liquid Chromatograph ◽  
Mobile Phase Flow Rate ◽  
Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

Development of HPLC Method for the Purity Test by Design of Experiments and Determination of Activation Energy of Hydrolytic Degradation Reactions of Sofosbuvir

2020 ◽  
Vol 16 (7) ◽  
pp. 976-987
Author(s):  
Jakub Petřík ◽  
Jakub Heřt ◽  
Pavel Řezanka ◽  
Filip Vymyslický ◽  
Michal Douša
Keyword(s):  
Activation Energy ◽  
Design Of Experiments ◽  
Mobile Phase ◽  
Hydrolytic Degradation ◽  
Isoconversional Method ◽  
Hplc Method ◽  
Organic Modifier ◽  
Calculation Methods ◽  
Degradation Reactions

Background: The present study was focused on the development of HPLC method for purity testing of sofosbuvir by the Design of Experiments and determination of the activation energy of hydrolytic degradation reactions of sofosbuvir using HPLC based on the kinetics of sofosbuvir degradation. Methods: Following four factors for the Design of Experiments were selected, stationary phase, an organic modifier of the mobile phase, column temperature and pH of the mobile phase. These factors were examined in two or three level experimental design using Modde 11.0 (Umetrics) software. The chromatographic parameters like resolution, USP tailing and discrimination factor were calculated and analysed by partial least squares. The chromatography was performed based on Design of Experiments results with the mobile phase containing ammonium phosphate buffer pH 2.5 and methanol as an organic modifier. Separation was achieved using gradient elution on XBridge BEH C8 at 50 °C and a flow rate of 0.8 mL/min. UV detection was performed at 220 nm. The activation energy of hydrolytic degradation reactions of sofosbuvir was evaluated using two different calculation methods. The first method is based on the slope of dependence of natural logarithm of the rate constant on inverted thermodynamic temperature and the second approach is the isoconversional method. Results and Conclusion: Calculated activation energies were 77.9 ± 1.1 kJ/mol for the first method and 79.5 ± 3.2 kJ/mol for the isoconversional method. The results can be considered to be identical, therefore both calculation methods are suitable for the determination of the activation energy of degradation reactions.

Development, Validation and Application of HPLC Method for Metformin in Rabbit Plasma

2019 ◽  
Vol 15 (6) ◽  
pp. 574-579
Author(s):  
Muhammad Ubaid ◽  
Mahmood Ahmad ◽  
Farhan Ahmad Khan ◽  
Ghulam Murtaza
Keyword(s):  
Flow Rate ◽  
Present Method ◽  
Mobile Phase ◽  
Hplc Method ◽  
Plasma Samples ◽  
Rabbit Plasma ◽  
Uv Detector ◽  
Reverse Phase ◽  
T Values ◽  
Pharmacokinetic Evaluation

Objective:This study was aimed at conducting a pharmacokinetic evaluation of metformin in rabbit plasma samples using rapid and sensitive HPLC method and UV detection.Methods:Acetonitrile was used for protein precipitation in the preparation of plasma samples. Reverse phase chromatography technique with silica gel column (250 mm × 4.6 mm, 5 μm) at 30°was used for the separation purpose. Methanol and phosphate buffer (pH 3.2) mixture was used as a mobile phase with flow rate 0.8 ml/min. The wavelength of UV detector was adjusted at 240 nm.Results:The calibration curve was linear in a range of 0.1-1 µg/ml with R² = 0.9982. The precision (RSD, %) values were less than 2%, whereas, accuracy of method was higher than 92.37 %. The percentage recovery values ranged between 90.14 % and 94.97 %. LOD and LOQ values were 25 ng/ml and 60 ng/ml, respectively. Cmax and AUC0-t values were found to be 1154.67 ± 243.37 ng/ml and 7281.83 ± 210.84 ng/ml.h, respectively after treating rabbits with a formulation containing 250 mg metformin.Conclusion:Based on the above findings, it can be concluded that present method is simple, precise, rapid, accurate and specific and thus, can be efficiently used for the pharmacokinetic study of metformin.

scholarly journals Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole

Separations ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Mohd Afzal ◽  
Mohd. Muddassir ◽  
Abdullah Alarifi ◽  
Mohammed Tahir Ansari
Keyword(s):  
Flow Rate ◽  
Mobile Phase ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Box Behnken Design ◽  
Rp Hplc ◽  
System Suitability ◽  
Method Optimization ◽  
Key Variables

A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100Å, column (250 × 4.6 mm, Phenomenex) with a mobile phase composed of acetonitrile/2 mM ammonium acetate (51:49 v/v), pH 6.7. The flow rate was 1 mL·min−1 with UV detection at 289 nm. Linearity was observed within the range of 4–36 µg·mL−1 for domperidone and 2–18 µg·mL−1 for lansoprazole. Method optimization was achieved using Box-Behnken design software, in which three key variables were examined, namely, the flow rate (A), the composition of the mobile phase (B), and the pH (C). The retention time (Y1 and Y3) and the peak area (Y2 and Y4) were taken as the response parameters. We observed that slight alterations in the mobile phase and the flow rate influenced the outcome, whereas the pH exerted no effect. Method validation featured various ICH parameters including linearity, limit of detection (LOD), accuracy, precision, ruggedness, robustness, stability, and system suitability. This method is potentially useful for the analysis of commercial formulations and laboratory preparations.

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