scholarly journals Sperm Quality and Oxidative-Antioxidant Profile in Men Living in Different Regions of Siberia

2020 ◽  
Vol 10 (4) ◽  
pp. 369-372
Author(s):  
Nadezhda Kurashova ◽  
Bair Dashiev ◽  
Lyubov Kolesnikova
Keyword(s):  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Reproductive Age ◽  
Anthropogenic Pressure ◽  
Factors Affecting ◽  
Antioxidant Profile ◽  
Caucasian Race ◽  
Geographical Position

Background: The aim of this study was to assess the sperm quality and oxidative-antioxidant profile in men living in different regions of Siberia Materials and Methods: The study involved 125 men of reproductive age: 40 men (mean age of 24.8 years) living in Ulan-Ude, 35 men (mean age of 24.1 years) in Irkutsk, and 50 men (mean age of 24.6 years) in Novosibirsk. All men belonged to the Caucasian race and were physically healthy. Methods of standard clinical examination of fertile and infertile men included: an ultrasonic scan of scrotum and prostate, macroscopic and microscopic examination of ejaculate, and biochemical analysis. The semen analysis was performed in accordance with the WHO recommendations (2010). The study of sperm quality included measuring the volume and pH of the ejaculate, the concentration of spermatozoa, the proportion of motile sperm of categories A and B, and lipid peroxidation-antioxidant activity products. Of the participants in the study, the group of somatically healthy men living in Irkutsk had the highest sperm count (mln per ml): 1.5 times more than men in Novosibirsk and 1.3 times more than men in Ulan-Ude. At the same time, in the group of men in Irkutsk, a significant increase in the concentration of α-tocopherol was revealed: 1.6 times more than the men in Ulan-Ude and 1.8 times more than the men in Novosibirsk. Conclusion: The conducted studies of the quality of ejaculate and the characteristics of LPO processes in men from different cities of Siberia show that place of residence and ecological-geographical position are not the main reasons determining reproductive dysfunction, but can be important factors affecting the functioning of the reproductive system and determining the heterogeneity of male infertility in conditions of anthropogenic pressure.

scholarly journals Influence of Lifestyle and Environmental Factors on Semen Quality in Ghanaian Men

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Richard Michael Blay ◽  
Abigail Duah Pinamang ◽  
Augustine E. Sagoe ◽  
Ewurama Dedea Ampadu Owusu ◽  
Nii Koney-Kwaku Koney ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Male Infertility ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Reproductive Age ◽  
Adult Males ◽  
Lifestyle Habits

Introduction. Male infertility is known to contribute about half of all infertility cases. In Ghana, the prevalence of male infertility is higher (15.8%) than in females (11.8%). Sperm quality is associated with the likelihood of pregnancy and known to be the cause of male fertility problems 90% of the time. Exposure to certain environmental factors reduces semen quality in men. The study examined the effects of environmental and lifestyle factors on semen quality in Ghanaian men. Materials and Methods. This was a cross-sectional study involving 80 apparent healthy adult males in their reproductive age. Participants were males referred to the laboratory (Immunology Unit of the Korle-Bu Teaching Hospital) for semen analysis test and/or culture and sensitivity. Participants were made to fill out a questionnaire which entailed selected environmental factors (accidents or trauma, exposure to chemicals, radiation, and heat) and lifestyle habits (including alcohol consumption, smoking, and whether participants sat more or less than 4 hours per day). Semen samples were then collected by masturbation into sterile containers and analysed in accordance with WHO guidance for semen analysis within 60 minutes after ejaculation and collection. Results. About 69% of participants had semen pH within the normal range compared to 15% whose pH were lower than 7.2. There was a significantly high number of immotile sperm cells ( p value = 0.017) in participants who sat for more than 4 hours as compared to those that sat for less than 4 hours in a day. Active sperm motility and viability showed significant increase ( p value = 0.002 and 0.009, respectively) in participants who kept their cell phones in their side pockets. Smoking produced a twofold decrease in sperm count as smokers had a significantly lower sperm count ( 12.28 ± 10.95 × 10 6 /ml) compared to the smoke-free ( 23.85 ± 22.14 × 10 6 /ml). For exposure to STDs, no significant differences were recorded among study groups concerning semen quality. Conclusion. Sperm quality in Ghanaian men is associated with lifestyle habits. Smoking and sitting for long hours influenced sperm motility and count, respectively. Knowledge of the factors that influence sperm quality in this geographical region can contribute to informed decisions on effective management of infertility in Ghanaian men.

scholarly journals HUMAN SPERM COUNT

2015 ◽  
Vol 22 (01) ◽  
pp. 013-017
Author(s):  
Jahangir Sultan ◽  
Kokab Kokab ◽  
Muneer Ahmed
Keyword(s):  
Environmental Factors ◽  
Human Activity ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Human Sperm ◽  
Factors Affecting ◽  
Parental Exposure ◽  
The World

Objectives: Reports from many parts of the world suggest that chemical andphysical agents in the environment, introduced and spread by human activity may affect fertilityin men. The objective of this article is to highlight the environmental factors and their associationto male sperm quality and count as well. Materials and Methods: This study focusing onexposure to environmental factors affecting the semen quality of the workers working in differentfactories for a period of 5 to 7 years in Lahore. Results: The results from this study suggest thatthere are many environmental factors which may affect semen quality and sperm count. It isnecessary to prevent parental exposure to the agents associated with those hazards.

Semen Analysis Profile on Varicocele Patients at Dr. Ramelan Navy Hospital Surabaya during Period 2015

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
TIFFANY WONGSODIHARJO
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Secondary Data ◽  
Married Couples ◽  
Sperm Cells ◽  
Descriptive Research ◽  
Normal Limits

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>: Infertility has affected about 10–15 percent married couples. Almost half of infertility cases have been attributed to men with low sperm motility (asthenozoospermia), low sperm count (oligozoospermia) and abnormal sperm morphology (teratozoospermia). Sperm motility is one of the major determinants of male fertility and is required for successful fertilization. Semen analysis is a method to know the quality of semen. Semen analysis consists of macroscopic and microscopic examination of sperm cells, which are concentration, motility and morphology of sperm cells. </p><p><strong>Objective</strong>:<strong> </strong>This study aimed to obtain the semen analysis profile on varicocele patients at DR. Ramelan Navy Hospital Surabaya during period 2015.</p><strong>Method</strong>: This study used descriptive research design and quantitative method, by using the secondary data were collected from medical records during January – December 2015. <strong>Result</strong>: In this study the semen analysis of 36 patients with varicocele, 3 patients (8,33%) with oligozoospermia, 10 patients (27,78%) with asthenozoospermia, 20 patients (55,55%) with oligoasthenozoospermia, 3 patients (8,33%) with extreme oligoasthenozoospermia. <strong>Conclusion</strong>: This study has concluded that all varicocele patients have abnormalities in semen analysis (none of the sperm quality results within normal limits).

Dietary Supplementation of Antioxidants Improves Semen Quality of IVF Patients in Terms of Motility, Sperm Count, and Nuclear Vacuolization

2012 ◽  
Vol 82 (6) ◽  
pp. 391-398 ◽  
Author(s):  
Barbara Wirleitner ◽  
Pierre Vanderzwalmen ◽  
Astrid Stecher ◽  
Dietmar Spitzer ◽  
Maximilian Schuff ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Class I ◽  
Antioxidative Therapy

Background: This study aimed to investigate the influence of an oral antioxidative supplementation on sperm quality of in vitro fertilization (IVF) patients, as analyzed by sperm motility according to the WHO criteria and motile sperm organelle morphology examination (MSOME). Methods: Semen samples were collected from 147 patients before undergoing an IVF/intracytoplasmic morphologically-selected sperm injection (IMSI) cycle and 2 - 12 months after an antioxidative supplementation. Semen analysis was evaluated according to WHO and MSOME criteria. Spermatozoa were grouped according to the size of nuclear vacuoles within the sperm’s heads. Patients were divided into oligoasthenoteratozoospermic (OAT) and non-OAT men. Between first and second semen analysis, patients were supplemented orally with an antioxidative preparation. Results: After the antioxidative therapy we observed a significant reduction in the percentage of immotile sperm cells in the patients. Additionally, the percentage of class I spermatozoa according to MSOME criteria was significantly higher after antioxidative supplementation. In OAT patients the percentage of class I sperm was found to be increased, although not significantly. However, we observed a drastic improvement in sperm motility as well as in total sperm count in this group. Conclusion: The results demonstrated a considerable improvement in semen quality, notably in OAT patients. Considering the putative relationship between semen quality on the one hand and reactive oxygen species on the other, the observed changes in the sperm parameters indicate that a decline in semen quality, and even subtle morphological changes, might be associated with oxidative stress. Our findings suggest that an antioxidative and micronutrient supplementation has a remarkable benefit for IVF patients having restricted sperm parameters, in particular.

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

The Potential Effect of Mucuna pruriens Seed Extract on Sperm Quality experimental study on mice exposed to cigarette smoke

2021 ◽  
Vol 20 (4) ◽  
pp. 768-773
Author(s):  
Meidona Nurul Milla ◽  
Yani Istadi ◽  
Vania Shaula ◽  
Deastri Anjeas Wari ◽  
Chntyia Dwi Cahyani Puspitasari ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cigarette Smoke ◽  
Sperm Quality ◽  
Medical Science ◽  
Seed Extract ◽  
Reproductive Age ◽  
Testicular Atrophy ◽  
Mucuna Pruriens ◽  
Control Group

Background: Infertility has been more common problems among couple of reproductive age. One of the factors causing this disorder is unhealthy environmental factors including exposure to cigarette smoke. Polynuclear aromatic hydrocarbons (PAHs) in cigarette smoke can cause testicular atrophy, while the free radicals can inhibit the stages of spermatogenesis, and nicotine in cigarettes affects the brain dopamine levels affecting the levels of GnRH, and subsequently affect the levels of FSH and LH needed in spermatogenesis. The use of Mucuna pruriens seed extract containing antioxidants and L-dopa is expected to improve the quality of sperm after exposure to cigarette smoke. Objective: This study aimed to determine the effect of Mucuna pruriens seed extract on the sperm quality in mice exposed to cigarette smoke. Methods: This study was an experimental study with a post test only control group design. A total of 20 mice were divided into 4 groups of five mice each. All groups were exposed to cigarette smoke. Group 1 was the negative control exposed to cigarette smoke. Groups 2, 3, 4 were exposed to cigarettes smoke and given Mucuna pruriens seed extracts at the dose of 250; 300; and 350 mg/Kg BW/day. Parameters of sperm quality included concentration, morphology, motility and viability. Results: Post hoc tests showed there were significant differences among treatment groups. Conclusion: the administration of Mucuna pruriens seed extract affects the sperm quality of BALB/c mice exposed to cigarettes smoke. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.768-773

scholarly journals iTRAQ-Based Sperm Proteomic Analyses Reveals Candidate Proteins Affecting the Quality of Spermatozoa From Boar on Plateaus

2020 ◽  
Author(s):  
Yanling Zhao ◽  
Yaomei Wang ◽  
Feipeng Guo ◽  
Bo Lu ◽  
Jiale Sun ◽  
...  
Keyword(s):  
Western Blot ◽  
Cellular Response ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Estrogen Signaling ◽  
Glutathione Metabolism ◽  
Computer Assisted ◽  
Expression Levels ◽  
Relative Expression

Abstract Background: Tibetan pigs (TP) exhibit heritable adaptations to their hypoxic environments as a result of natural selection. Whereas, what candidate proteins affecting the sperm quality of boar on plateaus has not been clearly investigated yet. Methods: In this study, to reveal the candidate proteins affecting the quality of spermatozoa from boar on plateaus, we analyzed the sperm quality by Computer-assisted semen analysis (CASA) system and Reactive oxygen species (ROS) levels, compared the sperm proteomes between TP and Yorkshire pigs (YP) raised at high altitudes using the isobaric tags for relative and absolute quantitation (iTRAQ) in combination with the liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic method, and confirmed the relative expression levels of the four proteins by western blot. Results: The sperm quality of the TP was superior to that of the YP on plateaus. Of 1,555 quantified proteins, 318 differentially expressed proteins (DEPs) were identified. Gene ontology (GO) analysis revealed that the DEPs were predominantly associated with the sorbitol metabolic process, removal of superoxide radicals, cellular response to superoxide, response to superoxide and regulation of the mitotic spindle assembly. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mainly enriched in pathways involved in the regulation of the actin cytoskeleton, glutathione metabolism, oxidative phosphorylation, and estrogen signaling. And based on the protein-protein interaction (PPI) network analysis, we identified 8 candidate proteins (FN1, EGF, HSP90B1, CFL1, GPX4, NDUFA6, VDAC2, and CP) that might play important roles that affect the sperm quality of boar on plateaus. Moreover, the relative expression levels of the proteins (CFL1, EGF, FN1, and GPX4) were confirmed by western blot. Conclusions: Our results reveal 8 candidate proteins (FN1, EGF, HSP90B1, CFL1, GPX4, NDUFA6, VDAC2, and CP) affecting the sperm quality of boar on plateaus, providing a reference for studies on improving sperm quality and the molecular breeding of boar on plateaus.

105 Increasing fertility of interspecific hybrid males using biotechnological approaches

2021 ◽  
Vol 33 (2) ◽  
pp. 159
Author(s):  
A. Vetokh ◽  
A. Tadzhieva ◽  
B. Iolchiev ◽  
N. Volkova ◽  
V. Bagirov
Keyword(s):  
Dna Fragmentation ◽  
Interspecific Hybrid ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Differential Staining ◽  
Computer Assisted ◽  
Sperm Dna Fragmentation ◽  
Russian Science

The results of AI depend on many factors, with the quality of semen being one of the most important. Not all male hybrids can meet the requirements for semen quality, because they often have reduced fertility following cryopreservation. Thus, it is necessary to improve semen processing before use in AI. The aim of the study was to evaluate the effectiveness of using the “swim-up” flotation method to improve sperm quality of hybrid males of the Ovis genus. Semen from interspecific hybrid rams (1/4 Argali×3/4 Romanov, n=15; 1/8 Argali×7/8 Romanov, n=15) was freshly obtained, frozen–thawed, and processed by the swim-up method. Evaluation of sperm motility was determined using computer-assisted semen analysis. Statistical analysis was performed using SPSS vs.15.0 (ANOVA and t-test; SPSS Inc.). Semen was collected during the breeding season (October–December) via artificial vagina. Assessment of acrosome integrity was determined using differential staining with a Diachem diff-quick kit (NPF ABRIS+). The degree of sperm DNA fragmentation was determined using the acridine-orange test. The sperm freezing/thawing cycle was accompanied by sperm damage and an increase in the proportion of immobile sperm from 10 to 58%, with non-progressive movement increasing from 9 to 19.3%. The number of spermatozoa with abnormal morphology doubled, and the DNA fragmentation index increased from 16 to 26%. Use of the swim-up procedure allowed us to sort progressively motile spermatozoa. The content of progressively motile spermatozoa in the samples obtained from the supernatant was 86%, which was 2.3 times higher than in frozen–thawed sperm (P≤0,01). The obtained results show the effective use of the swim-up procedure to determine the quality of semen in hybrid rams. These studies were carried out with financial support from the Russian Science Foundation, grant No. 18-16-00079 and the Ministry of Science and Higher Education of the Russian Federation.

scholarly journals Spermatotoxic effects of selected traditional alcoholic beverages from North-Central Nigeria on adult male albino rats

2021 ◽  
Vol 2 (2) ◽  
pp. 014-022
Author(s):  
Olusayo Moritiwon ◽  
Timothy Olugbenga Ogundeko ◽  
David Oyebode ◽  
James Bitrus ◽  
Adikpe Emmanuel Edugbe ◽  
...  
Keyword(s):  
Sperm Count ◽  
Reproductive Age ◽  
Alcoholic Beverages ◽  
Albino Rats ◽  
Normal Male ◽  
Sperm Cells ◽  
Negative Effects ◽  
North Central ◽  
Caudal Portion

Challenges associated with habitual intake of alcohol including health, social, psychological and especially reproductive health needs urgent attention. This study aimed to determine the spermatotoxic effect of selected traditional alcoholic beverages in rats. A total of 30 normal male Spaque dawley strain albino rats weighing 180-220g, divided into 5 groups of 6 rats in each were administered with 10ml/kg p.o each of pito, goskolo and ogogoro, goskolo respectively and 0.5ml/kg normal saline for a period of 21 days. Sperm samples were harvested from the left caudal portion epididymis assayed for sperm motility, sperm morphology and sperm count after which histological examination was carried out on the testes. Results showed that active, sluggish and dead sperm cells were goskolo>pito>burukutu>control>ogogoro, ogogoro> burukutu> control>goskolo>pito and control>pito>ogogoro>burukutu>goskolo respectively. For morphology of sperm cells, it was goskolo>ogogoro>burukutu>control>pito (normal) and pito>control>burukutu> ogogoro> goskolo (abnormal). Also, that of sperm count was goskolo>ogogoro>pito>burukutu>control. Results further showed that ogogoro and goskolo caused significant negative effects on quantity and quality of sperm cells with alteration of histological parameters marked with altered secondary spermatogonia and spermatid. These effects were however mild with pito and burukutu. Traditional alcoholic beverages from North central Nigeria ‘pito’, 'burukutu', 'ogogoro', and goskolo' have negative on the quantity and quality of sperm cells with marked with infraction of spermatogonia of male albino rats. As a result of the spermatotoxic properties of these locally available and often ingested drinks by males predominantly in the reproductive age brackets will do well to avoid and or minimize its use as it leads to reprotoxicity.

scholarly journals Current factors affecting the menstrual cycle

2020 ◽  
Vol 2 (1) ◽  
pp. 18-21
Author(s):  
Godari Akhila ◽  
Asra Shaik ◽  
R. Dinesh Kumar
Keyword(s):  
Menstrual Cycle ◽  
Lifestyle Factors ◽  
Reproductive Age ◽  
Menstrual Disorders ◽  
Important Indicator ◽  
Factors Affecting ◽  
Menstrual Patterns ◽  
The Impact ◽  
Skipping Breakfast

A regular menstrual cycle is an important indicator of a healthy reproductive system. Menstrual problems affect not only women but also families, social & national economy as well. The menstrual cycle is divided into two 14-day phases. In normal healthy women, menarche occurs between the ages 10 and 16 years, with an average rhythm of 28 days, inclusive of 4–6 days of bleeding, with some common minor variations like Amenorrhoea, Oligomenorrhoea, Polymenorrhoea, Menorrhagia, Hypomenorrhoea, etc., these menstrual disorders frequently affect the quality of life of young adults women, some of the lifestyle factors like obesity, intake of junk food, stress, lack of physical activity, skipping breakfast are associated with irregular menstruation. The purpose of this study is to identify menstrual irregularities and assess the impact of lifestyle factors and on the menstrual patterns in reproductive-age females.

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