scholarly journals DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card

2018 ◽  
Vol 3 (1) ◽  
pp. 1-3
Author(s):  
Elly L. Rustiati ◽  
Priyambodo Priyambodo ◽  
Siti Asiyah ◽  
Dedi Candra ◽  
Diah E. Anggraini ◽  
...  
Keyword(s):  
National Park ◽  
Dna Isolation ◽  
Species Conservation ◽  
Field Studies ◽  
Small Population ◽  
Molecular Profile ◽  
Genetic Profile ◽  
Training Center ◽  
Cold Condition ◽  
Way Kambas National Park

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’sID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.

THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Elly Lestari Rustiati ◽  
Priyambodo Priyambodo ◽  
Yanti Yulianti ◽  
Eko Agus Srihanto ◽  
Dian Neli Pratiwi ◽  
...  
Keyword(s):  
National Park ◽  
In Situ Conservation ◽  
Training Center ◽  
Conservation Effort ◽  
In The Wild ◽  
Wildlife Genetics ◽  
Way Kambas National Park ◽  
Human Conflict ◽  
Essential Contribution

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.

scholarly journals Comparative Cytogenetic Study on Male and Female Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park

2018 ◽  
Vol 3 (1) ◽  
pp. 4-6
Author(s):  
Priyambodo Priyambodo ◽  
Elly L. Rustiati
Keyword(s):  
National Park ◽  
Karyotype Analysis ◽  
Cytogenetic Study ◽  
Hypotonic Solution ◽  
Training Center ◽  
Male And Female ◽  
Fundamental Number ◽  
Conservation Genetic ◽  
Conservation Effort ◽  
Way Kambas National Park

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

scholarly journals Diversity of Avifauna at the Elephant Training Center Way Kambas National Park

2019 ◽  
Vol 7 (1) ◽  
pp. 10 ◽  
Author(s):  
Akhmad Kamaluddin ◽  
Gunardi Djoko Winarno ◽  
Bainah Sari Dewi
Keyword(s):  
Species Diversity ◽  
National Park ◽  
Bird Species ◽  
Bird Conservation ◽  
Training Center ◽  
Point Count ◽  
Count Method ◽  
Way Kambas National Park ◽  
Point Count Method

Avifauna is one of the biodiversity that has a role in the ecosystem, namely as a seed spreader, helps pollination, and as an environmental bioindicator. The Elephant Training Center (PLG) is not only a conservation centre for Sumatran elephants but also serves as a habitat and preserves the diversity of all animals including avifauna species. One form of conservation efforts towards avifauna is to record the diversity of the avifauna species present in that location. This study aims to determine the diversity of bird species found in the Way Kambas National Park PLG. The study was conducted using the point count method at three observation locations: garden area, grassland, and swamp area with three repetitions. The results of the study found 33 species from 21 families (727 individuals) with moderate species diversity values (1 <H '<3). The diversity of bird species affects the existence of some species in a habitat so that it affects the quality of habitat and plays an important role as a bioindicator in an ecosystem. Bird conservation efforts need to be carried out and supported by all parties to maintain the existence of birds in their natural habitat.Key words: Avifauna, diversity, conservation, national park, elephant training center.

scholarly journals ANALISIS OBYEK DAYA TARIK WISATA (ODTW) DAN INTERPRETASI JALUR EKOWISATA ELEPHANT TOUR DI PUSAT LATIHAN GAJAH TAMAN NASIONAL WAY KAMBAS

2021 ◽  
Vol 9 (2) ◽  
pp. 270
Author(s):  
Miftahudin Miftahudin ◽  
Gunardi Djoko Winarno ◽  
Trio Santoso ◽  
Arief Darmawan
Keyword(s):  
Spatial Analysis ◽  
National Park ◽  
Elephas Maximus ◽  
Field Observations ◽  
Training Center ◽  
Way Kambas National Park ◽  
Center Area ◽  
The Way

Way Kambas National Park is famous as an endemic habitat for the Sumatran Elephant (Elephas maximus sumatranus) precisely in the Elephant Training Center area as a base for ecotourism activities. Statistics on the number of tourist visits turned out to show a decrease, therefore we need an innovative eco-tourism breakthrough one of which has the potential to be developed is the Elephant Tour ecotourism. The purpose of this study is to identify potential attraction objects that exist in the Way Kambas National Park Elephant Training Center and interpret ecotourism path plans. The method used in this study is field observations to obtain data on potential points and ecotourism path. The data that has been obtained is then tabulated for descriptive and spatial analysis. The results showed that the object of attraction in the Way Kambas National Park precisely in the area of the Elephant Training Center consists of flora, fauna, beauty landscape and planned ecotourism path into two, long and short trips.

scholarly journals IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK

2021 ◽  
Vol 7 (1) ◽  
pp. 1-4
Author(s):  
Elsa Virnarenata ◽  
Elly Lestari Rustiati ◽  
Priyambodo Priyambodo ◽  
Eko Agus Srihanto ◽  
Dian Neli Pratiwi
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Distance ◽  
National Park ◽  
Dna Isolation ◽  
Coi Gene ◽  
Training Centre ◽  
Genetic Characteristics ◽  
Individual Female ◽  
Way Kambas National Park

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.

Wasur National Park Hall's Role In Raising The Local People's Income Wasur Village

2019 ◽  
Vol 2 (01) ◽  
pp. 44-52
Author(s):  
Paul Adryani Moento ◽  
Nikolaus Unawekla
Keyword(s):  
Forest Fires ◽  
Local Governments ◽  
National Park ◽  
Local Community ◽  
Counseling Center ◽  
Monitoring And Evaluation ◽  
Field Studies ◽  
Oil Refining ◽  
Salted Fish ◽  
Park Area

  The welfare level of the population in Wasur Park village is still very low and most still hang its life by taking the result of the forest. This study aims to analyze the role of Wasur Park National Park in increasing local people's income. The method by which researchers use a qualitative approach, while the data collection techniques consist of library studies and field studies through observation, interviews. The process of data analysis includes data reduction, data presentation, and the conclusion of the empowerment of the National Park Wasur Park Hall is counseling to the local community in the framework of the Empowerment Program of local Kampung Wasur Park. In the empowerment, Wasur Park National Park is coordinating with local governments to see the development of local community data. The real evidence of Wasur Park National Park through empowerment is the establishment of a rural forestry Counseling Center (SPKP). The establishment of a rural forestry Counseling Center (SPKP) in Wasur Village, implemented after the implementation of education and training of village studies in participatory. Furthermore, Wasur Park National Park Hall conducts construction. The construction is a construction of small industry of eucalyptus oil refining, the manufacture of salted fish, medicinal plants, and the cultivation of commercial crops. Then the community in providing coaching through socialization by providing science about preserving the forest and protection and safeguarding the potential of Wasur National Park area. Then Wasur Park National Park Hall conducts supervision. Supervision conducted by the National Park Hall Wasur Park is monitoring against the hunting of many protected animals located in Wasur Park National Park area. In the Garden Hall program, Wasur Park is monitoring and evaluation of habitat and population. The Wasur Park National Park Hall conducts a regular patrol, preventing wild hunters from Wasur Park National Park. Then patrol is also done to prevent forest fires.

scholarly journals Morphology and morphometry of adult nematodes on Sumatran elephants (Elephas maximus sumatranus) in Way Kambas National Park area, Indonesia

2019 ◽  
Vol 12 (2) ◽  
pp. 249-253 ◽  
Author(s):  
Rahmania Prahardani ◽  
Lintang Winantya Firdausy ◽  
Yanuartono ◽  
Wisnu Nurcahyo
Keyword(s):  
National Park ◽  
Female Worm ◽  
Lateral Lobe ◽  
Sem Analysis ◽  
Elephas Maximus ◽  
Electron Microscopic ◽  
Male Worm ◽  
Way Kambas National Park ◽  
Morphology And Morphometry ◽  
Park Area

Background and Aim: Worms from nematodes are the most numerous and the most detrimental in elephants. Most adult worms are located in the digestive tract. Nematode infection is at higher risk in young elephants, which caused several cases such as anemia, hypoalbuminemia, enteritis, and even death. This study aimed to determine the morphology and morphometry of adult nematodes on Sumatran elephants in Way Kambas National Park area. Materials and Methods: Nematode samples were obtained from Sumatran elephants' feces (Elephas maximus sumatranus) in Way Kambas National Park, Lampung Province, after being given Kalbazen® containing albendazole 1000 mg at a dose of 10 mg/kg by the veterinarian in charge of the National Park area. For the morphological and morphometric examinations, we used an Olympus BX 51 microscope equipped with Olympus DP 12 camera and were conducted at the Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Gadjah Mada. The scanning electron microscopic (SEM) analysis was carried out at the Biology Research Center of the Indonesian Institute of Sciences (Lembaga Ilmu Pengetahuan Indonesia). Results: The results of macroscopic observations of the obtained nematodes showed that the nematodes which were found have the characteristics of round, slim, and white color. The size of a female worm was larger than a male worm. Microscopic examination in four anterior papillae indicated that the dorsal lobe in the copulatory bursa was longer than lateral lobe. The result of inspection with the SEM showed a leaf crown consisting of 10 elements, a pair of amphids laterally, and two pairs of papilla in a submedian region. Conclusion: Based on our morphology and morphometry examinations of adult nematodes in Sumatran elephant (E. maximus sumatranus) in Way Kambas National Park area, the adult nematodes which were found are species of Quilonia travancra.

scholarly journals Mushrooms of Grand Teton National Park

1983 ◽  
Vol 7 ◽  
pp. 59-71
Author(s):  
Kent McKnight ◽  
Kimball Harper ◽  
Karl McKnight
Keyword(s):  
National Parks ◽  
Vascular Plants ◽  
National Park ◽  
Annual Report ◽  
Plant Cover ◽  
Field Studies ◽  
Soil Samples ◽  
Site Quality ◽  
Soil Parameters ◽  
Quantitative Estimates

The primary overall objective of inventorying the macrofungi growing in and around Grand Teton and Yellowstone National Parks was partially achieved with the published checklist (McKnight 1982) and additions from the 1982 Research Center Annual Report (McKnight, Harper, & McKnight 1984). The intensive collecting of the 1982 fruiting season including a 12-week phenological study at 11 selected sites left many species unidentified and numerous others observed but not collected, or with inadequately annotated collections made. Litter and soil moisture data for the 11 study stands are also given in the 1982 annual report cited above, as well as data on 15 overstory and understory vegetation and soil parameters. Field studies in the Parks during the summer of 1983 concentrated on (1) identification of chlorophyllous and vascular plants at the 11 sites selected for concentrated study in 1982; (2) quantitative estimates of chlorophyllous plant cover and height; (3) estimates of site quality; (4) collections of composite soil samples; and (5) additional records of macrofungi for the Parks with supporting data in the form of photographs, drawings, and annotations.

scholarly journals An in vivo multimodal feasibility study in a rat brain tumour model using flexible multinuclear MR and PET systems

2020 ◽  
Author(s):  
Chang-Hoon Choi ◽  
Carina Stegmayr ◽  
Aliaksandra Shymanskaya ◽  
Wieland A. Worthoff ◽  
Nuno A da Silva ◽  
...  
Keyword(s):  
Brain Tumour ◽  
Small Animal ◽  
Optimal Combination ◽  
Small Animal Pet ◽  
Molecular Profile ◽  
Genetic Profile ◽  
Normal Brain ◽  
Valuable Insight ◽  
Wide Range

Abstract Background : In addition to the structural information afforded by 1 H MRI, the use of X-nuclei, such as sodium-23 ( 23 Na) or phosphorus-31 ( 31 P), offers important complementary information concerning physiological and biochemical parameters. By then combining this technique with PET, which provides valuable insight into a wide range of metabolic and molecular processes by using of a variety of radioactive tracers, the scope of medical imaging and diagnostics can be significantly increased. While the use of multimodal imaging is undoubtedly advantageous, identifying the optimal combination of these parameters to diagnose a specific dysfunction is very important and is advanced by the use of sophisticated imaging techniques in specific animal models.Methods : In this pilot study, rats with intracerebral 9L gliosarcomas were used to explore a combination of sequential multinuclear MRI using a sophisticated switchable coil set in a small animal 9.4 T MRI scanner and, subsequently, a small animal PET with the tumour tracer O-(2-[ 18 F]-fluoroethyl)-L-tyrosine ( 18 F-FET). This enabled in vivo multinuclear MR-PET experiments to be conducted without compromising the performance of either multinuclear MR or PET.Results : High-quality in vivo images and spectra including high-resolution 1 H imaging, 23 Na-weighted imaging, detection of 31 P metabolites and 18 F-FET uptake were obtained, allowing the characterisation of tumour tissues in comparison to a normal brain. These parameters have been shown to be useful in the identification of the genetic profile of gliomas, particularly concerning the mutation of the isocitrate hydrogenase gene, which is highly relevant for treatment strategy.Conclusions : The combination of multinuclear MR and PET in, for example, brain tumour models with specific genetic mutations will enable the physiological background of signal alterations to be explored and the identification of the optimal combination of imaging parameters for the non-invasive characterisation of the molecular profile of tumours.

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