scholarly journals The Effect of Bull Age and Ejaculation Frequency on Quality of Aceh Bull Spermatozoa

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Dini Melita ◽  
Dasrul Dasrul ◽  
Mulyadi Adam
Keyword(s):  
Analysis Of Variance ◽  
Volume Concentration ◽  
Frequency Effect ◽  
Bull Semen ◽  
Frozen Semen ◽  
Semen Volume ◽  
Ejaculation Frequency ◽  
Effect Of Age ◽  
Artificial Vagina

The purpose of this research was find out the effect of age and ejaculation frequency on quality of Aceh bull spermatozoa.. This research was conducted at Frozen Semen Prossesing Laboratorium, Saree, Aceh Besar from Januari to April 2013. Six healthy aceh bulls were divided into two groups of age, those were 3.0-4.0 years old; and 4.0-5.0 years old. Three numbers of bull at each group were assumed as the replication. Semen was collected once a week using artificial vagina and collected until third semen ejaculation. After collecting, volume, pH, color, consistency, concentration, motility, mortality, and abnormality of spermatozoa were immediately measured. This data was analyzed by analysis of variance (ANOVA) two-way pattern, followed by Duncant test. The result showed that the evaluation of bull semen at 3.0-4.0 years old and 4.0-5.0 years old affect significantly (P0.05) on semen volume and spermatozoa concentration and had no contribute significantly on motility, mortality and abnormality of spermatozoa,while ejaculation frequency effect significantly (P0.05) on semen volume, concentration, and abnormality spermatozoa, but had no effect significantly on motility and mortality (P0.05). It is concluded that aged and ejaculation frequency effect significantly on quality of aceh bull fresh spermatozoa.Key words: ejaculation frequency, quality of spermatozoa, aceh bull, age

scholarly journals The The Effect of Age and Breed on The Quality of Bull Semen in The Regional Artificial Insemination Centre

2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Age Groups ◽  
Bull Semen ◽  
Sperm Abnormalities ◽  
Frozen Semen ◽  
Cattle Reproduction ◽  
Effect Of Age ◽  
Artificial Vagina

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.

Effect of genetic and environmental factors on semen productionand quality of Madura cattle in Indonesia

2016 ◽  
Author(s):  
Koko Wisnu Prihatin ◽  
Luqman Hakim ◽  
Sucik Maylinda ◽  
V.M. Ani Nurgiartiningsih
Keyword(s):  
Environmental Factors ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Climatic Conditions ◽  
Bull Semen ◽  
Semen Collection ◽  
Semen Volume ◽  
Ejaculation Frequency ◽  
Genetic And Environmental Factors

The indigenous Madura cattle semen production and quality were evaluated. Total of 2275 semen collection records from 11 bulls were analyzed to evaluate the genetic potency of semen productions. The genetic evaluation was described as an estimation of bull semen volume, sperm concentration and sperm individual motility. Data were analyzed by HGLM-REML with environmental factors of age, seasons, interval of semen collections and frequency of ejaculations as fixed effect. The result showed that the age and the ejaculation frequency affected to all variables; an interval of semen collections affected to both semen volume and sperm concentrations; and seasons only affected to sperm concentrations. Repeatability estimate of semen volume, sperm concentration and sperm individual motility were 0.376, 0.445 and 0.567 respectively. It can be concluded that the quality of ejaculate was less affected by environmental factors, supported by the good adaptation of indigenous Madura cattle under the local climatic conditions and management in the artificial insemination station.

scholarly journals Kualitas semen sapi pejantan berdasarkan umur, suhu, dan kelembaban di Taman Ternak Pendidikan Universitas Airlangga

2021 ◽  
Vol 10 (3) ◽  
pp. 81
Author(s):  
Febby Fairy Brillianti ◽  
Pudji Srianto ◽  
Trilas Sardjito ◽  
Tri Wahyu Suprayogi ◽  
Indah Norma Triana ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Effect Of Temperature ◽  
Storage Process ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Temperature And Humidity ◽  
Effect Of Age ◽  
Fresh Semen ◽  
Artificial Vagina

This research was conducted at the Teaching Farm of the Faculty of Veterinary Medicine, Airlangga University, to know the effect of age, temperature, and humidity on the quality of semen of bulls which are turned into frozen semen. This research uses fresh semen samples of bulls of various ages grouped into 3 groups, young, middle age, and old groups and measures temperature and humidity during the storage process. Fresh semen is collected using an artificial vagina and then examined macroscopic and microscopic tests which is then checked for post thawing motility. Data analysis uses Analysis of Variants (ANOVA) followed by Duncan. The results showed that the percentage of spermatozoa motility of fresh semen and post thawing differed and age affected the quality of semen produced. There was no effect of temperature and humidity when processing of collecting semen. Bull’s semen at Teaching Farm Faculty of Veterinary Medicine Airlangga University has good quality and is suitable for Artificial Insemination. 

scholarly journals Kualitas Semen Segar Sapi Bali Umur Produktif dan Non-produktif serta Penentuan Konsentrasi Krioprotektan dalam Pengencer Tris Kuning Telur

2018 ◽  
Vol 19 (2) ◽  
pp. 242 ◽  
Author(s):  
Anna Nabilla ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Dimethyl Formamide ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Bull Semen ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different. 

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

scholarly journals THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN

2017 ◽  
Vol 41 (1) ◽  
pp. 1 ◽  
Author(s):  
Moh Takdir ◽  
Ismaya Ismaya ◽  
Sigit Bintara
Keyword(s):  
Egg White ◽  
Egg Albumin ◽  
Randomized Design ◽  
Frozen Semen ◽  
Bottom Fraction ◽  
Completely Randomized Design ◽  
Ram Sperm ◽  
Artificial Vagina ◽  
Range Test

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan’s Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen.  (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y) 

scholarly journals Penambahan PGF2α dalam pengencer semen dapat meningkatkan motilitas pasca thawing motility spermatozoa domba Waringin

2021 ◽  
Vol 19 (2) ◽  
pp. 210
Author(s):  
Husnurrizal Husnurrizal ◽  
Ade Syahriani Aritonang ◽  
Tongku Nizwan Siregar ◽  
Teuku Armansyah ◽  
Hafizuddin Hafizuddin
Keyword(s):  
Analysis Of Variance ◽  
Freezing Process ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Additional Amount ◽  
Completely Randomized Design ◽  
Artificial Vagina

<p class="MDPI17abstract"><strong>Objective: </strong>The purpose of this research was to find out the rise of spermatozoa motility of Waringin sheep which was given additional amount of PGF2α in commercial semen diluents (Andromed).</p><p class="MDPI17abstract"><strong>Methods: </strong>A total of 3 Waringin sheep aged 2-3 years were used in this study. Semen samples were collected using an artificial vagina and evaluation of the quality of the spermatozoa was carried out macroscopically and microscopically. This study used a unidirectional completely randomized design. After evaluated, semen samples were added with Andromed diluents afterwards divided into three groups treatment, K1; K2; and K3 where each group was then added with physiologic NaCl, 37,5 µg PGF2α, and 75 µg PGF2α. All of the samples were equilibrated in refrigerator for 4 hours and spermatozoa motility was examined, then after the freezing process was carried out and stored for 24 hours, the post thawing spermatozoa motility was examined. The data obtained was analyzed using one-way analysis of variance (ANOVA) and followed by Duncan test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result of the research showed that motility of post equilibration spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 43,67±1,20; 75,00±4,00; and 82,33 ±3,18 (P&lt;0,05); and motility of post thawing spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 25,33±1,85; 49,33±3,17; dan 54,00 ±5,50 (P&lt;0,05).<strong></strong></p><strong>Conclusions: </strong>In conclusion that was the rise in motility of prefreezing spermatozoa and motility of post thawing spermatozoa of Waringin sheep which given additional amount of PGF2α on Andromed diluents.

scholarly journals Comparison of Quality of Bull Semen Collected in the Artificial Vagina and by Electroejaculation

1961 ◽  
Vol 44 (12) ◽  
pp. 2292-2297 ◽  
Author(s):  
J.W. Austin ◽  
E.W. Hupp ◽  
R.L. Murphree
Keyword(s):  
Bull Semen ◽  
Artificial Vagina

scholarly journals Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1506
Author(s):  
Hongtao Wang ◽  
Ping Lu ◽  
Chongshan Yuan ◽  
Jing Zhao ◽  
Hongyu Liu ◽  
...  
Keyword(s):  
Semen Analysis ◽  
Computer Assisted ◽  
Astragalus Polysaccharides ◽  
Bovine Semen ◽  
Bull Semen ◽  
Straight Line ◽  
Frozen Semen ◽  
Path Distance ◽  
Straight Line Distance

The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis (CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (p < 0.05), while reducing the ROS and MDA levels (p < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.

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