Study on the killing effect of cold atmospheric pressure plasma on MRSA Staphylococcus aureus in vitro and in vivo infection model

Despite increasing knowledge gained based on multidisciplinary research, plasma medicine still raises various questions regarding specific effects as well as potential risks. With regard to significant statements about in vivo applicability that cannot be prognosticated exclusively based on in vitro data, there is still a deficit of clinical data. This study included a clinical follow-up of five probands who had participated five years previously in a study on the influence of cold atmospheric pressure plasma (CAP) on the wound healing of CO2 laser-induced skin lesions. The follow-up included a complex imaging diagnostic involving dermatoscopy, confocal laser scanning microscopy (CLSM) and hyperspectral imaging (HSI). Hyperspectral analysis showed no relevant microcirculatory differences between plasma-treated and non-plasma-treated areas. In summary of all the findings, no malignant changes, inflammatory reactions or pathological changes in cell architecture could be detected in the plasma-treated areas. These unique in vivo long-term data contribute to a further increase in knowledge about important safety aspects in regenerative plasma medicine. However, to confirm these findings and secure indication-specific dose recommendations, further clinical studies are required.

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Influence of Microwave Frequency and Gas Humidity on the In-Vitro Blood Coagulation in Cold Atmospheric Pressure Plasma

Processes ◽

10.3390/pr9101837 ◽

2021 ◽

Vol 9 (10) ◽

pp. 1837

Author(s):

Jie Yu ◽

Li Wu ◽

Kama Huang

Keyword(s):

Blood Coagulation ◽

Atmospheric Pressure ◽

Blood Clotting ◽

Atmospheric Pressure Plasma ◽

Emission Spectra ◽

Optical Emission ◽

Microwave Frequency ◽

Pressure Plasma ◽

Cold Atmospheric Pressure Plasma

In this article, the effects of microwave frequency (2450 MHz and 5800 MHz) and gas humidity (1%, 2%, 3%, 4%, 6% and 8%) on in vitro blood coagulation with cold atmospheric pressure plasma (CAPP) were investigated. The generation of reactive oxygen species (ROS, OH, O) was measured by optical emission spectra. The exposure temperature on blood droplets under treatment was below 55 °C in all cases, to avoid the thermal effect of plasma on the blood clotting. Investigations showed that, with the increase of frequency, the doses of ROS increased, the blood sample presented a more serious collapse and its surface became drier. The humidity of ionized gas can also accelerate the generation of ROS and the process of blood clotting. Our results propose a method to accelerate in vitro blood coagulation in CAPP by adjusting microwave frequency and gas humidity, and suggest a clinical benefit for plasma treatment as a coagulation device in surgery.

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Cold atmospheric pressure plasma (CAPP) as a new alternative treatment method for onychomycosis caused by Trichophyton verrucosum: in vitro studies

Infection ◽

10.1007/s15010-021-01691-w ◽

2021 ◽

Author(s):

Sebastian Gnat ◽

Dominik Łagowski ◽

Mariusz Dyląg ◽

Jessica Zielinski ◽

Marek Studziński ◽

...

Keyword(s):

Alternative Treatment ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Treatment Method ◽

In Vitro Studies ◽

Trichophyton Verrucosum ◽

Promising Alternative ◽

Pressure Plasma ◽

Cold Atmospheric Pressure Plasma

Abstract Purpose Anthropophilic dermatophytes as etiological factors of onychomycoses are more common than zoophilic fungi. In the case of the latter, reverse zoonoses are possible, which poses a threat to the persistence of dermatophytes in the environment. Nevertheless, without treatment, both types of tinea unguium may lead to complete nail plate destruction and secondary mixed infections with fungi and bacteria. One of the zoophilic dermatophytes that cause onychomycosis is Trichophyton verrucosum, whose prevalence has been increasing in recent years. Such infections are usually treated with allylamines and/or azoles, but such a conventional treatment of infections caused by T. verrucosum often fails or is discontinued by patients. Methods Herein, we reveal the results of our in vitro studies related to direct application of cold atmospheric pressure plasma (CAPP) on Trichophyton verrucosum growth, germination and adherence to nail as a new alternative treatment method of such types of dermatomycoses. Results Our in vitro studies showed that, while exposure to CAPP for 10 min delays germination of conidia and clearly impairs the fitness of the fungal structures, 15 min is enough to kill all fungal elements exposed to plasma. Moreover, the SEM images revealed that T. verrucosum cultures exposed to CAPP for 10 and 15 min were not able to invade the nail fragments. Conclusion The results revealed that single exposure to CAPP was able to inhibit T. verrucosum growth and infection capacity. Hence, cold atmospheric pressure plasma should be considered as a promising alternative treatment of onychomycoses.

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Long-lived and short-lived reactive species produced by a cold atmospheric pressure plasma jet for the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2018.05.083 ◽

2018 ◽

Vol 124 ◽

pp. 275-287 ◽

Cited By ~ 38

Author(s):

V.S. Santosh K. Kondeti ◽

Chi Q. Phan ◽

Kristian Wende ◽

Helena Jablonowski ◽

Urvashi Gangal ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Plasma Jet ◽

Reactive Species ◽

Atmospheric Pressure Plasma Jet ◽

Pressure Plasma ◽

Cold Atmospheric Pressure Plasma ◽

And Staphylococcus Aureus

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Cold Atmospheric-Pressure Plasma Caused Protein Damage in Methicillin-Resistant Staphylococcus aureus Cells in Biofilms

Microorganisms ◽

10.3390/microorganisms9051072 ◽

2021 ◽

Vol 9 (5) ◽

pp. 1072

Author(s):

Li Guo ◽

Lu Yang ◽

Yu Qi ◽

Gulimire Niyazi ◽

Lingling Huang ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Atmospheric Pressure ◽

Methicillin Resistant Staphylococcus Aureus ◽

Atmospheric Pressure Plasma ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Multidrug Resistant Bacteria ◽

Methicillin Resistant ◽

Pressure Plasma ◽

Cold Atmospheric Pressure Plasma

Biofilms formed by multidrug-resistant bacteria are a major cause of hospital-acquired infections. Cold atmospheric-pressure plasma (CAP) is attractive for sterilization, especially to disrupt biofilms formed by multidrug-resistant bacteria. However, the underlying molecular mechanism is not clear. In this study, CAP effectively reduced the living cells in the biofilms formed by methicillin-resistant Staphylococcus aureus, and 6 min treatment with CAP reduced the S. aureus cells in biofilms by 3.5 log10. The treatment with CAP caused the polymerization of SaFtsZ and SaClpP proteins in the S. aureus cells of the biofilms. In vitro analysis demonstrated that recombinant SaFtsZ lost its self-assembly capability, and recombinant SaClpP lost its peptidase activity after 2 min of treatment with CAP. Mass spectrometry showed oxidative modifications of a cluster of peaks differing by 16 Da, 31 Da, 32 Da, 47 Da, 48 Da, 62 Da, and 78 Da, induced by reactive species of CAP. It is speculated that the oxidative damage to proteins in S. aureus cells was induced by CAP, which contributed to the reduction of biofilms. This study elucidates the biological effect of CAP on the proteins in bacterial cells of biofilms and provides a basis for the application of CAP in the disinfection of biofilms.

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