Study on Enhanced Oil Recovery Using Microorganism Generating Foam in Presence of Nanobubbles

2021 ◽  
Author(s):  
Miu Ito ◽  
Yuichi Sugai
Keyword(s):  
Carbon Dioxide ◽  
Protein Concentration ◽  
Culture Medium ◽  
Tap Water ◽  
Culture Solution ◽  
Oil Reservoir ◽  
Ferrous Ions ◽  
Microbial Cells ◽  
Foam Generation ◽  
Positively Charged

Abstract Both high cost and environmental load of surfactant are issues to be solved in foam EOR. Moreover, it is difficult to control the injection of surfactant and gas so that the foam is generated in only high permeable zones selectively in oil reservoir. The authors have found a foam generating microorganism and hit upon an idea of the microbial foam EOR which makes the microorganism do generating foam in oil reservoir. The mechanism of the microbial foam generation and culture condition suitable for the foam generation were studied in this study. A species of Pseudomonas aeruginosa was used as a foam producer in this study. It was cultured in the medium consisting of glucose and eight kinds of minerals at 30 °C and atmospheric pressure under anaerobic conditions. Because P. aeruginosa generally grows better under aerobic conditions, the microorganism was supplied with oxygen nanobubbles as the oxygen source. The carbon dioxide nanobubbles were also used as a comparison target in this study. The state of foam generation in the culture solution was observed during the cultivation. The surface tension, surfactant concentration, protein concentration, polysaccharides concentration and bacterial population of the culture solution were measured respectively. The foam was started to be generated by the microorganism after 2 days of cultivation and its volume became maximum after 3 days of cultivation. The foam generation was found in the culture solution which contained both oxygen nanobubbles and carbon dioxide nanobubbles whereas little foam was found in non-nanobubbles culture solution. The foam generation found in the culture solution containing carbon dioxide nanobubbles was more than that in the culture solution containing oxygen nanobubbles. Both gas and protein concentration increased along with the formation of the foam whereas surfactant and polysaccharides were not increased, therefore, the foam was assumed to be generated with gas and protein which were generated by P. aeruginosa. It was found that the carbon dioxide nanobubbles were positively charged in the culture medium whereas they were negatively charged in tap water through the measurement of zeta potential of nanobubbles, therefore, the carbon dioxide nanobubbles attracted cations in the culture medium and became positively charged. Positively charged carbon dioxide nanobubbles transported cations to the microbial cells of P. aeruginosa. Among cations in the culture medium, ferrous ions are essential for the protein generation of P. aeruginosa, therefore, the positively charged carbon dioxide nanobubbles attracted ferrous ions and transport them to the microbial cells, resulting the growth and metabolism of P. aeruginosa were activated. Those results suggest that the microbial foam EOR can be materialized by supplying the microorganism with carbon dioxide nanobubbles or ferrous ions.

scholarly journals Nanobubbles activate anaerobic growth and metabolism of Pseudomonas aeruginosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miu Ito ◽  
Yuichi Sugai
Keyword(s):  
Carbon Dioxide ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Anaerobic Condition ◽  
Culture Medium ◽  
Cell Concentration ◽  
Essential Elements ◽  
Anaerobic Growth ◽  
Ferrous Ions ◽  
Positively Charged

AbstractThe effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. P. aeruginosa grew earlier in the culture medium containing nanobubbles and the bacterial cell concentration in that culture medium was increased a few times higher compared to the medium without nanobubbles under anaerobic condition. Both gas and protein, which are the metabolites of P. aeruginosa, were remarkably produced in the culture medium containing nanobubbles whereas those metabolites were little detected in the medium without nanobubbles, indicating nanobubbles activated anaerobic growth and metabolism of P. aeruginosa. The carbon dioxide nanobubbles came to be positively charged by adsorbing cations and delivered ferrous ions, one of the trace essential elements for bacterial growth, to the microbial cells, which activated the growth and metabolism of P. aeruginosa. The oxygen nanobubbles activated the activities of P. aeruginosa as an oxygen source.

Experimental and Numerical Modeling of Gaseous Carbon Dioxide Injecting Into Aqueous Water in Bottle Filler System

2001 ◽  
Author(s):  
Y. H. Zheng ◽  
R. S. Amano
Keyword(s):  
Carbon Dioxide ◽  
Numerical Modeling ◽  
Tap Water ◽  
Co2 Injection ◽  
Operating Characteristics ◽  
Two Phase ◽  
Carbonation Process ◽  
Filling System ◽  
Gas System ◽  
Gaseous Carbon Dioxide

Abstract An efficient enhancement of the carbonation rate in the bottle filling stage can substantially increase the production in beverage industries. The bottle filling system currently used in most of the manufacturers can still be improved for a better performance of carbonation by designing the injection tube system. This paper reports on an experimental and numerical mass transfer modeling that can simulate the dissolution process of gaseous carbon dioxide into aqueous water in the bottle filler system. In order to establish the operating characteristics of the bottle filler system, an ordinary tap water and pure carbon dioxide were used as the liquid-gas system. The two-phase numerical modeling was developed that can serve as a framework for the continuous improvement of the design of the carbonation process in the bottle filler system. For an optimal design of CO2 injection tube and flow conditions, a computational fluid dynamics (CFD) approach is one of the most power tools. However, since only limited experimental data are available in the open literature to verify the computational results, an experiment study was performed to obtain measurements of CO2 level, temperature, and pressure during the carbonation process in the bottle filled with liquid. Both experimental and numerical studies of various flow condition and different sizes of injection tube are presented in this paper.

SIGNIFICANT EFFECT OF PH ON PHOTOCATALYTIC DEGRADATION OF ORGANIC POLLUTANTS USING SEMICONDUCTOR CATALYSTS

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Nur Farhana Jaafar ◽  
Aishah Abdul Jalil ◽  
Sugeng Triwahyono ◽  
Adnan Ripin ◽  
Mohamad Wijayanuddin Ali
Keyword(s):  
Carbon Dioxide ◽  
Methyl Orange ◽  
Physicochemical Properties ◽  
Photocatalytic Degradation ◽  
Organic Pollutants ◽  
Ph Value ◽  
Effect Of Ph ◽  
Electrolysis Method ◽  
Ph Range ◽  
Positively Charged

Photocatalytic is one of the inexpensive and non-toxic techniques for degradation of organic pollutants into harmless substances such as water and carbon dioxide. In this study, simple electrolysis method was used in preparation of Ag/TiO2 and α-Fe2O3/HY catalysts. The physicochemical properties of the catalysts were studied using XRD, FTIR, FESEM-EDX and surface area analysis. The pH of solution plays an important role in the photocatalytic degradation of organic pollutants which influences the surface-charge properties of the catalysts. Ag/TiO2 and α-Fe2O3/HY were used as catalyst on degradation of 2-chlorophenol (2-CP) and methyl orange (MO), respectively. The effect of pH on degradation of 2-CP and MO were investigated over a pH range from 2 to 9. Higher degradation of 2-CP and MO were obtained at pH 5 (74%) and pH 2 (80%), respectively. This finding might be explained by the amphoteric performance of the catalyst using point zero charge (pHZPC). The pHZPC for Ag/TiO2 and α-Fe2O3/HY was found to be at pH 6.3 and pH 7.2, respectively. Hence, the activities of the catalysts may have been affected by the existence of a strong electrostatic field between the positively charged catalysts surface and negatively charged 2-CP and MO caused a pH value lower than their pHZPC give greater degradation.

Mass Transfer Process of Gaseous Carbon Dioxide Into Water Jet Through Orifice Mixing System

2000 ◽  
Author(s):  
Y. H. Zheng ◽  
R. S. Amano
Keyword(s):  
Carbon Dioxide ◽  
Mass Transfer ◽  
Transfer Process ◽  
Tap Water ◽  
Mass Transfer Process ◽  
Operating Characteristics ◽  
Water Stream ◽  
Gas System ◽  
Junction Type ◽  
Gaseous Carbon Dioxide

Abstract This paper summarizes the mass transfer modeling that can simulate the process of gaseous carbon dioxide dissolution into water in an orifice mixing system. In order to establish the operating characteristics of the orifice mixing system, ordinary tap water and pure carbon dioxide were used as the liquid-gas system. Using the model, computations were performed for an orifice mixing system to better understand the mass transfer process of gaseous carbon dioxide into water through both the elbow tube and the junction Venturi-tube. All computed results show different performance of the carbon dioxide dissolution rates for the given inlet water and carbon dioxide conditions of the four different designs of the junction type Venturi-tubes and an orifice mixing system. After examining the computed results it was found that the mass transfer efficiency of gaseous carbon dioxide into the water stream through the orifice mixing system was superior to that through the junction Venturi-tubes.

scholarly journals The Position of Solid Carbon Dioxide in the Triboelectric Series

2019 ◽  
Vol 72 (8) ◽  
pp. 633 ◽  
Author(s):  
Jinyang Zhang ◽  
Simone Ciampi
Keyword(s):  
Carbon Dioxide ◽  
Negative Charge ◽  
Plastic Material ◽  
Indirect Evidence ◽  
Liquid Carbon ◽  
Solid Carbon Dioxide ◽  
Dry Ice ◽  
First Time ◽  
Charge Sign ◽  
Positively Charged

The process of releasing liquid carbon dioxide from a fire extinguisher is accompanied by a strong static charging of the plastic material making up the extinguisher discharge horn. Firefighters often report an electric shock when operating CO2 extinguishers, but the origin of this electrostatic hazard is largely unknown. Here, we begin to investigate this phenomenon, and test the hypothesis of plastic samples being tribocharged on contact with rapidly flowing solid CO2. Using Faraday pail measurements, we show that non-conductive polymers gain a net static charge when brought in and out of contact with dry ice (solid CO2). These measurements of charge sign and magnitude give indirect evidence helping to place solid CO2 for the first time on the triboelectric series. Polydimethylsiloxane (PDMS), polytetrafluoroethylene (PTFE), and polyvinyl chloride (PVC) samples acquire a negative charge when rubbed against dry ice, whereas poly(methyl methacrylate) (PMMA), glass, and nylon surfaces become positively charged. Therefore, we suggest the position of dry ice in the triboelectric series to be close to that of materials with stable cations and unstable anions, possibly locating it between PMMA and PVC.

scholarly journals FORMATION OF TRYPSIN FROM TRYPSINOGEN BY AN ENZYME PRODUCED BY A MOLD OF THE GENUS PENICILLIUM

1938 ◽  
Vol 21 (5) ◽  
pp. 601-620 ◽  
Author(s):  
M. Kunitz
Keyword(s):  
Temperature Coefficient ◽  
Acid Medium ◽  
Experimental Error ◽  
Protein Concentration ◽  
Liquid Culture ◽  
Culture Medium ◽  
Specific Activity ◽  
Crystalline Form ◽  
Liquid Culture Medium ◽  
Autocatalytic Activation

1. A powerful kinase which changes trypsinogen to trypsin was found to be present in the synthetic liquid culture medium of a mold of the genus Penicillium. 2. The concentration of kinase in the medium is increased gradually during the growth of the mold organism and continues to increase for some time even after the mold has ceased growing. 3. Mold kinase transforms trypsinogen to trypsin only in an acid medium. It differs thus from enterokinase and trypsin which activate trypsinogen best in a slightly alkaline medium. 4. The action of the mold kinase in the process of transformation of trypsinogen is that of a typical enzyme. The process follows the course of a catalytic unimolecular reaction, the rate of formation of a definite amount of trypsin being proportional to the concentration of kinase added. The ultimate amount of trypsin formed, however, is independent of the concentration of kinase used. 5. The formation of trypsin from trypsinogen by mold kinase is not accompanied by any measurable loss of protein. 6. The temperature coefficient of formation of trypsin from trypsinogen by mold kinase varies from Q5–15 = 1.70 to Q25–30 = 1.25 with a corresponding variation in the value of µ from 8100 to 4250. 7. Trypsin formed from trypsinogen by means of mold kinase is identical in crystalline form with the crystalline trypsin obtained by spontaneous autocatalytic activation of trypsinogen at pH 8.0. The two products have within the experimental error the same solubility and specific activity. A solution saturated with the crystals of either one of the trypsin preparations does not show any increase in protein concentration or activity when crystals of the other trypsin preparation are added. 8. The Penicillium mold kinase has a slight activating effect on chymo-trypsinogen the rate being only 1–2 per cent of that of trypsinogen. The activation, as in the case of trypsinogen, takes place only in an acid medium. 9. Mold kinase is rapidly destroyed when brought to pH 6.5 or higher, and also when heated to 70°C. In the temperature range of 50–60°C. the inactivation of kinase follows a unimolecular course with a temperature coefficient of Q10 = 12.1 and µ = 53,500. The molecular weight of mold kinase, as determined by diffusion, is 40,000.

THE DEVELOPMENT OF THE TIRRAWARRA OIL AND GAS FIELD

1984 ◽  
Vol 24 (1) ◽  
pp. 278
Author(s):  
H. T. Pecanek ◽  
I. M. Paton
Keyword(s):  
Carbon Dioxide ◽  
Oil And Gas ◽  
Gas Injection ◽  
Oil Field ◽  
Oil Reservoir ◽  
Gas Reservoirs ◽  
Gas Field ◽  
Associated Gas ◽  
Oil And Gas Field ◽  
Cooper Basin

The Tirrawarra Oil and Gas Field, discovered in 1970 in the South Australian portion of the Cooper Basin, is the largest onshore Permian oil field in Australia. Development began in 1981 as part of the $1400 million Cooper Basin Liquids ProjectThe field is contained within a broad anticline bisected by a north-south sealing normal fault. This fault divides the Tirrawarra oil reservoir into the Western and Main oil fields. Thirty-four wells have been drilled, intersecting ten Patchawarra Formation sandstone gas reservoirs and the Tirrawarra Sandstone oil reservoir. Development drilling discovered three further sandstone gas reservoirs in the Toolachee Formation.The development plan was based on a seven-spot pattern to allow for enhanced oil recovery by miscible gas drive. The target rates were 5400 barrels of oil (860 kilolitres) per day with 13 million ft3 (0.37 million m3) per day of associated gas and 70 million ft3 (2 million m') per day of wet, non-associated gas. Evaluation of early production tests showed rapid decline. The 100 ft (30 m) thick, low-permeability Tirrawarra oil reservoir was interpreted as an ideal reservoir for fracture treatment and as a result all oil wells have been successfully stimulated, with significant improvement in well production rates.The oil is highly volatile but miscibility with carbon dioxide has been proven possible by laboratory tests, even though the reservoir temperature is 285°F (140°C). Pilot gas injection will assess the feasibility of a larger-scale field-wide pressure maintenance scheme using miscible gas. Riot gas injection wells will use Tirrawarra Field Patchawarra Formation separator gas to defer higher infrastructure costs associated with the alternative option of piping carbon dioxide from Moomba, the nearest source.

Geological storage of carbon dioxide by injection of carbonated water in an Iranian oil reservoir: A case study

2013 ◽  
Vol 111 ◽  
pp. 170-177 ◽  
Author(s):  
Mahdi zeinali Hasanvand ◽  
Mohammad Ali Ahmadi ◽  
Seyed Reza Shadizadeh ◽  
Reza Behbahani ◽  
Farzaneh Feyzi
Keyword(s):  
Carbon Dioxide ◽  
Oil Reservoir ◽  
Geological Storage ◽  
Carbonated Water
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