scholarly journals Peripheral CD4CD8 Double Positive T Cells: A Potential Marker to Evaluate Kidney Damage Susceptibility During SLE

2020 ◽  
Author(s):  
Kai Chang ◽  
Yanyan Wang ◽  
Chenxia Liu ◽  
Wanlin Na ◽  
Hongxuan Xu ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Kidney Damage ◽  
Dependent Manner ◽  
Double Positive ◽  
Potential Marker ◽  
Nephritic Syndrome ◽  
Biochemical Measurements ◽  
Damage Susceptibility ◽  
Cell Proportion

Abstract Background: Lupus nephritis (LN) has a high incidence in Systemic lupus erythematosus (SLE) patients, but there is a lack of sensitive predictive markers and mechanisms. The purpose of the study is to reveal the association between the CD4+CD8+ double positive T lymphocytes (DPT) and lupus nephritis (LN), and to investigate the immune mechanism of LN. Methods: The study is composed of 395 samples from the General Hospital of Western Theater Command. Collected patients include SLE, lupus nephritis (LN), nephritic syndrome (NS) and nephritis patients. Peripheral blood lymphocyte subsets were performed by the Flow cytometry method. Biochemical measurements were performed in accordance with the recommendations proposed by national center for clinical laboratories.Results: The proportions of DPT cells in LN group were significantly higher than in SLE group (t=4.012, p<0.001), NS group (t=3.240, p=0.001) and nephritis group (t=2.57, p=0.011). In LN group, the risk of kidney damage increased significantly in the DPT cell proportion dependent manner. In cases of high DPT cells proportion, the risk of LN was 5.136 times higher than when the proportion DPT cell was within the normal range. Moreover, hypertriglyceridemia and hyperuricemia were also independent risk factors.Conclusion: The proportion of DPT cells was a potential marker to evaluate LN susceptibility. When assessing the risk of kidney damage during SLE with DPT cell proportion, we can effectively exclude the interference of NS and nephritis.

scholarly journals Lupus Nephritis In Systemic Lupus Erythematosus

2020 ◽  
Vol 02 (10) ◽  
pp. 145-150
Author(s):  
Nazarova Nigina Otabek Qizi ◽  
◽  
Jabbarov Azim Atakhanovich ◽  
Abduhalikova Nigora Fahriddinovna ◽  
Madazimova Dilrux Xayotjonovna ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Treatment Strategy ◽  
Kidney Damage ◽  
Systemic Lupus ◽  
Morphological Classification ◽  
Morphological Variant ◽  
Clinical Variants

The pathogenesis, clinic, and treatment of kidney damage in patients with systemic lupus erythematosus (SLE) are considered. It is noted that if at the beginning of the disease signs of kidney damage are present in 25-50% of SLE patients, then later they are detected in almost 60% of adults and 80% of children. Variants of kidney damage in SLE are described. The pathogenesis of SLE is generally considered on the model of lupus nephritis. The morphological classification of lupus nephritis, features of the main nephrological syndromes, and clinical variants (active and inactive) are presented. It is indicated that the treatment strategy depends on the activity of the disease, the clinical and morphological variant of lupus nephritis.

scholarly journals Involvement of infiltrating macrophage-derived activin A in the progression of renal damage in MRL-lpr mice

2017 ◽  
Vol 312 (2) ◽  
pp. F297-F304 ◽  
Author(s):  
Anastasie Tshilela Kadiombo ◽  
Akito Maeshima ◽  
Ken Kayakabe ◽  
Hidekazu Ikeuchi ◽  
Toru Sakairi ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Renal Damage ◽  
Transforming Growth Factor ◽  
Intraperitoneal Administration ◽  
Transforming Growth Factor Β ◽  
Activin A ◽  
Dependent Manner ◽  
Cytokines And Chemokines

Lupus nephritis is a life-threatening complication of systemic lupus erythematosus (SLE). Various growth factors, cytokines, and chemokines are implicated in the development of SLE. However, the pathophysiological processes involved in the development of lupus nephritis still remain unclear. In this study, we examined the involvement of activin A, a member of the transforming growth factor β (TGF-β) superfamily, in the progression of renal damage in lupus-prone MRL- lpr mice. Activin A was not expressed in the kidneys of control MRL-MpJ mice but was detectable in perivascular infiltrating cluster of differentiation 68 (CD68)-positive cells in the kidneys of MRL- lpr mice. Urinary activin A, which was also absent in MRL-MpJ mice, was detectable in MRL- lpr mice from 16 wk onward. Urinary activin A levels were significantly correlated with the number of perivascular inflammatory cell layers, the number of crescentic glomeruli, and the percentage of Elastica van Gieson (EVG)-positive fibrotic areas, but not with urinary protein levels or serum activin A. When activin action was blocked in vivo by the intraperitoneal administration of an activin antagonist, follistatin, the number of crescentic glomeruli, percentage of EVG-positive fibrotic areas, CD68-positive cell infiltration, and proteinuria were significantly reduced in a dose-dependent manner. These data suggest that infiltrating macrophage-derived activin A is involved in the progression of renal damage in MRL- lpr mice.

scholarly journals Serum Cytokines Th1, Th2, and Th17 Expression Profiling in Active Lupus Nephritis-IV: From a Southern Chinese Han Population

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Keshav Raj Sigdel ◽  
Lihua Duan ◽  
Yin Wang ◽  
Weiping Hu ◽  
Ning Wang ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Active Form ◽  
Cell Immune Response ◽  
Interstitial Tissue ◽  
Class Iii ◽  
Serum Cytokines ◽  
Southern Chinese ◽  
Potential Marker ◽  
Th17 Cytokines

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by aberrant T cell immune response. Diffuse proliferative lupus nephritis (LN-IV) is the most common, severe, and active form of lupus nephritis. In this study, we investigated the production of Th1, Th2, and Th17 cytokines in prediction of active form of LN-IV. ProcartaPlex multiplex immunoassays panels were used for detection of serum Th1, Th2, and Th17 cytokines profiling. Th1 and Th17 cytokines (IL-18, IFN-γ, IL-12p70, IL-6, and IL-17A) were considerably expressed in the serum of lupus nephritis IV patients in comparison to the healthy control. However, only IL18 and IL6 were higher in class IV versus class III lupus nephritis. Importantly, the ratios of Th1/Th2 (IL-18/IL-4) and Th17/Th2 (IL-17A/IL-4) were significantly elevated in LN-IV when compared with LN-III, LN-V, and healthy controls. Consistently, the serum cytokines IL-18, IL-17A, and IFN-γwere markedly expressed in LN-IV patient glomeruli and interstitial tissue compared to other classes of LN by IHC. ROC further suggests that IL-18 was a potential marker for LN-IV. The data from our study suggests that the early detection and quantification of these cytokines may help in prediction of active form of LN-IV.

scholarly journals Basophils as a potential marker of lupus nephritis by flow cytometry

2021 ◽  
pp. FSO690
Author(s):  
Yanni Jiang ◽  
Jin Chen ◽  
Yi Zhao ◽  
Yi Liu ◽  
Hong Xu ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Flow Cytometry ◽  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Mononuclear Cells ◽  
Lymphoid Cells ◽  
Healthy Controls ◽  
Systemic Lupus ◽  
Cross Sectional ◽  
Potential Marker

Objective: To establish a convenient and simple flow cytometry immunophenotyping panel to explore immune cellular alterations and potential cellular biomarkers in systemic lupus erythematosus. Materials and methods: This is a cross-sectional, case–control study including 60 patients with systemic lupus erythematosus and 20 sex- and age-matched healthy controls. A 14-color immunophenotyping panel was applied to detect proportions of circulating immune mononuclear cells, and comparisons between patients and healthy controls, and subgroups of patients, were performed. Correlations between cellular proportions and other parameters were investigated. Results: After multivariate analysis, significantly decreased proportions of CD4−CD8− T cells, natural killer cells and innate lymphoid cells were observed in patients compared with healthy controls. The proportions of basophils were decreased significantly in patients with lupus nephritis (LN) compared with those in patients without LN. Conclusion: In the present study, we found that basophil proportions may be a biomarker of LN.

scholarly journals KIDNEY BIOPSY – AN IMPORTANTTOOL FOR THE DIAGNOSIS, TREATMENT AND DETERMINATION OF THE PROGNOSIS OF PATIENTS WITH LUPUS NEPHRITIS

2020 ◽  
Vol 25 ◽  
pp. 26-31
Author(s):  
Violetta Dubovik
Keyword(s):  
Lupus Nephritis ◽  
Renal Biopsy ◽  
Lupus Erythematosus ◽  
Kidney Biopsy ◽  
Clinical Signs ◽  
Kidney Damage ◽  
Molecular Characteristics ◽  
Diagnosis And Prognosis ◽  
Personalized Approach ◽  
Vague Data

Introduction. Kidney damage can vary from mild to severe and occurs in 50% -70% of patients with systemic lupus erythematosus (SLE) [4], more often develops during 2-3 years [2]. Lupus nephritis (LN) has various morphological manifestations with different clinical signs and consequences. Percutaneous kidney biopsy is the gold standard for the diagnosis of LN according to the WHO classification since 1974 [1]. The purpose. This review reveals the importance of kidney biopsy for diagnosis and prognosis. Material and methods. Review of contemporary and foreign literary sources; techniques – description, analysis, abstracting. Results. There are many reports of clinical using of the renal biopsy classification of LN, but the number of studies evaluating its validity is not yet sufficient. Renal biopsy of LN provides vague data, which raises serious doubts about validity and clinical use, because it can lead to serious errors during diagnosis, treatment and prognosis. Conclusions. The molecular analysis of the bioptate will be the key to a personalized approach to drug selection in LN. The molecular characteristics of kidney damage available with LN will provide the necessary supplements to more accurately classify the disease, predict outcomes, and further use of new therapeutics.

Correlation Between Serological Makers and Immunofluorescence Deposits i n Kidney Tissue of Patients with Lupus Nephritis

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Haider S Al-Hadad ◽  
Aqeel Abbas Matrood ◽  
Maha Abdalrasool Almukhtar ◽  
Haider Jabur Kehiosh ◽  
Riyadh Muhi Al-Saegh
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Kidney Biopsy ◽  
Pearson Correlation ◽  
Standard Procedure ◽  
Kidney Tissue ◽  
P Value ◽  
American College Of Rheumatology ◽  
Immune Deposits ◽  
Number Of Patients

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease. Few biomarkers for SLE have been validated and widely accepted for the laboratory follow-up of inflammatory activity. In SLE patients, with lupus nephritis (LN), complement activation leads to fluctuation of serum C3 and C4 that are frequently used as clinicalm biomarker of disease activity in SLE. Patients and Methods: In this study the number of patients were 37, seven patients were excluded for incomplete data collection, 28 were females ,2 were males. The duration of the study is two years from 2015 to 2017. Patients were considered to have SLE and LN according to American College of Rheumatology (ACR) criteria, and International Society of Nephrology/ Renal Pathology Society (ISN/RPS). All patients were evaluated withm clinical presentation, laboratory investigations. Our patients underwent kidney biopsy according to standard procedure by Kerstin Amann, and their tissue specimens were studied in the laboratory with light microscope (LM) and immunofluorescence microscope reagents. The relationship between the serological markers and immunofluorescence deposits in kidney biopsy of all patients were studied using the statistical analysis of Pearson correlation and single table student's T test. A P value 0.05 was considered statistically significant. Results: The granular pattern of IF deposits was present in all LN patients, and in more than two third of patients these IF deposits presented in glomerular, tubular, and mesangium sites. While less than one third of patients had IF deposits in the mesangium only. There was no statistically significant correlation between serum ANA, anti-dsDNA, and IF deposits of different types. There was significant correlation between serum C3 and C4 hypocomplementemia and IgG immune deposits in kidney biopsy, and there was significant relationship between serum C3 hypocomplementemia and full house immunofluorescence (FHIF) deposits inm kidney biopsy.Conclusions:Immunofluorescence deposits is mainly granular pattern in LN patients. There was no significant association between serum ANA, anti-dsDNA, and immune deposits in kidney tissue. Immunofluorescence deposits of IgG type correlates significantly with serum C3 and C4 hypocomplemetemia, and these immune deposits in association with low complement levels correlates with LN flare. There was significant correlation between C3 hypocomplementemia and FHIF.

THE CLINICAL, LABORATORY MANIFESTATIONS AND HISTOPATHOLOGY IN NEPHROTIC PATIENTS WITH LUPUS NEPHRITIS

2018 ◽  
pp. 52-58
Author(s):  
Le Thuan Nguyen ◽  
Bui Bao Hoang
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Clinical Laboratory ◽  
Activity Index ◽  
Clinical Manifestations ◽  
Systemic Lupus ◽  
Cross Sectional ◽  
Organ Systems ◽  
Tubulointerstitial Lesions

Introduction: Systemic lupus erythematosus (SLE) is an autoimmune disease involving multiple organ systems. The kidney appears to be the most commonly affected organ, especially nephrotic is a serious kidney injury. The clinical, laboratory manifestations and histopathology are very useful for diagnosis, provide the means of predicting prognosis and guiding therapy in nephrotic patients with lupus nephritis. Methods: Descriptive cross-sectional study of nephrotic patients with lupus treated in the Department of Nephrology Trung Vuong Hospital and Cho Ray Hospital between May/2014 and May/2017. Renal histopathological lesions were classified according to International Society of Nephrology/Renal Pathology Society - ISN/RPS ’s 2003. The clinical, laboratory manifestations and histopathological features were described. Results: Of 32 LN with nephritic range proteinuria cases studied, 93.7% were women. The 3 most common clinical manifestations were edema (93.8%), hypertension (96.8%) and pallor (68.9%), musculoskeletal manifestions (46.9%), malar rash (40.6%). There was significant rise in laboratory and immunological manifestions with hematuria (78.1%), Hb < 12g/dL (93.5%), increased Cholesterol (100%), and Triglycerid (87.5%), Creatinine > 1.4 mg/dL (87.5%), increased BUN 71.9%, ANA (+) 93.8%, Anti Ds DNA(+) 96.9%, low C3: 96.9%, low C4: 84.4%. The most various and severe features were noted in class IV with active tubulointerstitial lesions and high activity index. Conclusion: Lupus nephritis with nephrotic range proteinuria has the more severity of histopathological feature and the more severity of the more systemic organ involvements and laboratory disorders were noted. Key words: Systemic lupus, erythematosus (SLE) lupus nepphritis, clinical

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