Expression of Penaeidins 3a in Transgenic Duckweed Confers Antibacterial Ability

Abstract Background With the development of aquaculture, fish and shrimp diseases have been paid more and more attention in the world. How to improve the immunity of aquatic animals was an urgent problem to be solved. Duckweed (Lemnacecae), as a eukaryote, could be an ideal feedstock for the production of antimicrobial peptides. Result Penaeidins 3a (Pen 3a) from Litopenaeus vannamei was expressed under the control of CaMV-35S promoter in duckweed, Lemna turionifera 5511. Bacteriostatic test by Pen3a duckweed extract showed the antibacterial activity against Escherichia coli and Staphylococcus aureus. Transcriptome analysis of WT and Pen3a duckweed showed different results, and the protein metabolic process was the most up-regulated DEGs. In Pen 3a transgenic duckweed, the expression of sphingolipid metabolism and phagocytosis process-related genes have been significantly up-regulated. Quantitative proteomics suggested a remarkable difference in protein enrichment in metabolic pathways. Conclusion Our study provide a novel solution on aquaculture and water purification. The Pen 3a transgenic duckweed extraction inhibit the growth of gram-negative bacteria, gram-positive bacteria, which could be applied to control the bacteria in lake. The results could lay the foundation for the subsequent production of antibiotics.

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N-Doped Carbon Quantum Dots as Fluorescent Bioimaging Agents

Crystals ◽

10.3390/cryst11070789 ◽

2021 ◽

Vol 11 (7) ◽

pp. 789

Author(s):

Shih-Fu Ou ◽

Ya-Yun Zheng ◽

Sin-Jen Lee ◽

Shyi-Tien Chen ◽

Chien-Hui Wu ◽

...

Keyword(s):

Quantum Dots ◽

Carbon Nanomaterials ◽

Graphene Quantum Dots ◽

Carbon Quantum Dots ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Fluorescence Characteristics ◽

And Staphylococcus Aureus

Graphene quantum dots, carbon nanomaterials with excellent fluorescence characteristics, are advantageous for use in biological systems owing to their small size, non-toxicity, and biocompatibility. We used the hydrothermal method to prepare functional N-doped carbon quantum dots (N-CQDs) from 1,3,6-trinitropyrene and analyzed their ability to fluorescently stain various bacteria. Our results showed that N-CQDs stain the cell septa and membrane of the Gram-negative bacteria Escherichia coli, Salmonellaenteritidis, and Vibrio parahaemolyticus and the Gram-positive bacteria Bacillus subtilis, Listeria monocytogenes, and Staphylococcus aureus. The optimal concentration of N-CQDs was approximately 500 ppm for Gram-negative bacteria and 1000 ppm for Gram-positive bacteria, and the exposure times varied with bacteria. N-Doped carbon quantum dots have better light stability and higher photobleaching resistance than the commercially available FM4-64. When excited at two different wavelengths, N-CQDs can emit light of both red and green wavelengths, making them ideal for bioimaging. They can also specifically stain Gram-positive and Gram-negative bacterial cell membranes. We developed an inexpensive, relatively easy, and bio-friendly method to synthesize an N-CQD composite. Additionally, they can serve as a universal bacterial membrane-staining dye, with better photobleaching resistance than commercial dyes.

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Inhibition of Aspergillus flavus and Selected Gram-positive Bacteria by Chelation of Essential Metal Cations by Polyphosphates

Journal of Food Protection ◽

10.4315/0362-028x-54.5.360 ◽

1991 ◽

Vol 54 (5) ◽

pp. 360-365 ◽

Cited By ~ 31

Author(s):

S.J. KNABEL ◽

H.W. WALKER ◽

P.A. HARTMAN

Keyword(s):

Aspergillus Flavus ◽

Binding Sites ◽

Iron Supplementation ◽

Cation Binding ◽

Gram Negative Bacteria ◽

Positive Bacterium ◽

Gram Positive ◽

Tetrasodium Pyrophosphate ◽

Gram Positive Bacteria ◽

And Staphylococcus Aureus

A simple well-plate technique was utilized to determine the effect of various metals on the growth of microorganisms in media containing different polyphosphates. Aspergillus flavus and four gram-positive bacteria were completely inhibited by media containing 1% of various alkaline polyphosphates, whereas four gram-negative bacteria were not. Significant differences were observed between the type of polyphosphate added, the type of metal added, and the species of gram-positive bacterium inhibited. The addition of Mg2+ stimulated growth of A. flavus and Bacillus cereus in the presence of tetrasodium pyrophosphate, whereas Mn2+ permitted growth of A. flavus and Staphylococcus aureus in the presence of sodium hexametaphosphate. Iron supplementation allowed the growth of S. aureus and Listeria monocytogenes on media containing 1 % tetrasodium pyrophosphate. A method for determining the amount of calcium and magnesium in water was modified to detect free Mg2+ by replacing EDTA with phosphate. The addition of free Mg2+, but not Mg2+ chelated by tetrasodium pyrophosphate, permitted the growth of B. cereus on a medium containing tetrasodium pyrophosphate. It is speculated that polyphosphates specifically inhibited A. flavus and gram-positive bacteria by removing essential metals from cation-binding sites located within their cell walls.

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NMR Hydrophilic Metabolomic Analysis of Bacterial Resistance Pathways using Multivalent Antimicrobials with Challenged and Unchallenged Wild Type and Mutated Gram-Positive Bacteria

International Journal of Molecular Sciences ◽

10.3390/ijms222413606 ◽

2021 ◽

Vol 22 (24) ◽

pp. 13606

Author(s):

Michelle L. Aries ◽

Mary J. Cloninger

Keyword(s):

Metabolic Pathways ◽

Qualitative Method ◽

Bacterial Resistance ◽

Proton Nuclear Magnetic Resonance ◽

Gram Negative Bacteria ◽

Wild Type ◽

Gram Positive ◽

Resistance Development ◽

Gram Positive Bacteria ◽

Positively Charged

Multivalent membrane disruptors are a relatively new antimicrobial scaffold that are difficult for bacteria to develop resistance to and can act on both Gram-positive and Gram-negative bacteria. Proton Nuclear Magnetic Resonance (1H NMR) metabolomics is an important method for studying resistance development in bacteria, since this is both a quantitative and qualitative method to study and identify phenotypes by changes in metabolic pathways. In this project, the metabolic differences between wild type Bacillus cereus (B. cereus) samples and B. cereus that was mutated through 33 growth cycles in a nonlethal dose of a multivalent antimicrobial agent were identified. For additional comparison, samples for analysis of the wild type and mutated strains of B. cereus were prepared in both challenged and unchallenged conditions. A C16-DABCO (1,4‑diazabicyclo-2,2,2-octane) and mannose functionalized poly(amidoamine) dendrimer (DABCOMD) were used as the multivalent quaternary ammonium antimicrobial for this hydrophilic metabolic analysis. Overall, the study reported here indicates that B. cereus likely change their peptidoglycan layer to protect themselves from the highly positively charged DABCOMD. This membrane fortification most likely leads to the slow growth curve of the mutated, and especially the challenged mutant samples. The association of these sample types with metabolites associated with energy expenditure is attributed to the increased energy required for the membrane fortifications to occur as well as to the decreased diffusion of nutrients across the mutated membrane.

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Synthesis of Some Derivatives of 4-phenyl-1,3-dihydro-2H-imidazole-2-thion Using Ionic Liquid as Catalyst and Evaluation of Their Antimicrobial Activity

International Journal of Chemistry ◽

10.5539/ijc.v9n2p45 ◽

2017 ◽

Vol 9 (2) ◽

pp. 45 ◽

Cited By ~ 1

Author(s):

Bahareh Behmaram ◽

Naser Foroughifar ◽

Neda Foroughifar ◽

Sara Hallajian

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Ionic Liquid ◽

13C Nmr Spectroscopy ◽

Basic Medium ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Derivatives Of ◽

And Staphylococcus Aureus

The synthesis of some 1,3-diazoles and thiazoles was realized in different conditions:a) In the presence of PTSA or sulfuric acid as catalyst we obtained only diazole products(4a-d).b) In basic medium such as DABCO or sodium hydroxide and ionic liquid afforded thiazoles.c) Both products, diazoles and thiazoles were collected when using methanol as catalyst and solvent.All structures were confirmed by IR, 1H NMR and 13C NMR spectroscopy. The antibacterial activity of some synthesized compounds was investigated against Escherichia Coli (ATCC: 25922) and Serratia marcescens (ATCC: 13880) as gram negative bacteria, Bacillus sabtilis (ATCC: 6633) and Staphylococcus aureus (ATCC: 6338) as gram positive bacteria. Some of these products exhibit good activities to significant antibacterial activity.

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Three New Abietane-Type Diterpenoids from Plectranthus africanus and Their Antibacterial Activities

Planta Medica ◽

10.1055/s-0043-114426 ◽

2017 ◽

Vol 84 (01) ◽

pp. 59-64 ◽

Cited By ~ 2

Author(s):

Raïssa Nzogong ◽

Blaise Nganou ◽

Alex Tedonkeu ◽

Maurice Awouafack ◽

Mathieu Tene ◽

...

Keyword(s):

Minimal Inhibitory Concentration ◽

Inhibitory Concentration ◽

Methanol Extract ◽

2D Nmr ◽

Antibacterial Activities ◽

Gram Negative Bacteria ◽

Gram Positive Bacteria ◽

Whole Plant ◽

New Compounds ◽

And Staphylococcus Aureus

AbstractThree new abietane-type diterpenoids, plectranthroyleanones A – C (1 – 3), together with five known compounds (4 – 8) were isolated from the methanol extract of the whole plant of Plectranthus africanus using column chromatography techniques. The structures of the new compounds were elucidated using a combination of 1D and 2D NMR and HRESIMS analyses. Compound 1 exhibited weak activities with minimal inhibitory concentration values of 75 µg/mL against gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, and 150 µg/mL against two gram-negative bacteria, Pseudomonas aeruginosa and Klebsiella pneumoniae, respectively, while 2 and 3 had moderate antibacterial activity against K. pneumoniae with a minimal inhibitory concentration value of 37.5 µg/mL.

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ANTIBACTERIAL ACTIVITY OF BARK EXTRACTS OF FICUS GLOMERATA ROXB AGAINST SOME GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

INDIAN DRUGS ◽

10.53879/id.50.05.p0044 ◽

2013 ◽

Vol 50 (05) ◽

pp. 44-47

Author(s):

S. D. Bhinge ◽

◽

M. G Hogade ◽

A. S Savali ◽

H. R. Chitapurkar ◽

...

Keyword(s):

Antibacterial Activity ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Aqueous Extracts ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Agar Diffusion Method ◽

Ficus Glomerata ◽

And Staphylococcus Aureus

Petroleum ether, ethanol and aqueous extracts of the bark of Ficus glomerata (Urticaceae) were screened for their antibacterial activity using agar diffusion method. They were tested against six bacteria; three Gram-positive bacteria (Bacillus subtilis, Bacillus aureus and Staphylococcus aureus) and three Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa). The susceptibility of the microorganisms to the extracts of these plants was compared with each other and with selected antibiotics.

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Synthesis, Characterization and Antibacterial Activity of New Derivatives of Imidazolidine having Sulfamethoxazole Moiety

Al Mustansiriyah Journal of Pharmaceutical Sciences ◽

10.32947/ajps.19.04.0433 ◽

2019 ◽

pp. 217-238 ◽

Cited By ~ 1

Keyword(s):

Antibacterial Activity ◽

Schiff Bases ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Acinetobacter Species ◽

Gram Positive Bacteria ◽

Ft Ir ◽

Preliminary Study ◽

Derivatives Of ◽

And Staphylococcus Aureus

This work includes Synthesis of new series of sulfa drugs derived from sulfamethoxazole containing substituted Imidazolidine moiety. These compounds expected to have antibacterial activity, due to their Imidazolidine moiety This work includes synthesis of some new Schiff bases (II1-5) by condensation of sulfamethoxazole drug(I) with some aldehydes (1-5) (benzaldehyde, p-chloro benzaldehyde, p-nitro benzaldehyde, p-hyroxy benzaldehyde and p-N, N-dimethyl amino benzaldehyde). These Schiff bases were found to react with glycine, to prepared new imidazolidine derivatives (III1-5). The prepared compounds were characterized by physical properties, FT-IR and of the 1H-NMR spectroscopy. The preliminary study of antibacterial activity of final compounds has considered by well diffusion method. The tested compounds displayed effect against gram negative bacteria:( Acinetobacter species and Pseudomonas aeruginosa) and gram-positive bacteria (Streptococcus pyougenes and Staphylococcus aureus bacteria), which compared to DMSO as control, and good activity compared to sulfamethoxazole as standard.

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Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v43i1.52011 ◽

2021 ◽

Vol 43 ◽

pp. e52011

Author(s):

Alan Kelbis Oliveira Lima ◽

Arthur Abinader Vasconcelos ◽

Renata Katsuko Takayama Kobayashi ◽

Gerson Nakazato ◽

Hugo de Campos Braga ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Gram Negative Bacteria ◽

Leaf Extracts ◽

Gram Positive Bacteria ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Clinical Interest ◽

The Mean ◽

And Staphylococcus Aureus

This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest.

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Synthesis, characterization and antibacterial activity of Methyl (2R)-2-benzamido-2-{[(1R)-2-methoxy-2-oxo- 1-phenylethyl]amino}acetate

Mediterranean Journal of Chemistry ◽

10.13171/mjc74181110-alami ◽

2018 ◽

Vol 7 (4) ◽

pp. 267-271

Author(s):

Oumaima Karai ◽

Younas Aouine ◽

Hassane Faraj ◽

Alami Anouar ◽

Mohammed Rachid Kabbour ◽

...

Keyword(s):

Antibacterial Activity ◽

Methylene Chloride ◽

Bactericidal Effect ◽

Gram Negative Bacteria ◽

X Ray Diffraction ◽

Prepared Compound ◽

X Ray ◽

Gram Positive Bacteria ◽

And Staphylococcus Aureus

The present work covers the recent synthetic of methyl (2R)-2-benzamido-2-{[(1R)-2-methoxy-2-oxo-1-phenylethyl]amino}acetate, via N-alkylation of methyl a-azido glycinate N-benzoylated with methyl 2-amino-2-phenylacetate in methylene chloride and in presence of triethylamine as basic catalyst. The structure of the prepared compound was determined by spectroscopic methods: 1H-NMR, 13C-NMR, MS data, elemental analysis and confirmed by X-Ray diffraction. This compound was screened in vitro for its antibacterial activity against Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella enteric). The MIC values confirmed that the title compound had a bactericidal effect against the strains tested.

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Evaluation of Ruthenium(II) N-Heterocyclic Carbene Complexes as Antibacterial Agents and Inhibitors of Bacterial Thioredoxin Reductase

Molecules ◽

10.3390/molecules26144282 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4282

Author(s):

Hilke Burmeister ◽

Pascal Dietze ◽

Lutz Preu ◽

Julia E. Bandow ◽

Ingo Ott

Keyword(s):

General Type ◽

Antibacterial Agents ◽

Thioredoxin Reductase ◽

Gram Negative Bacteria ◽

Antibacterial Effects ◽

Carbene Complexes ◽

Metal Free ◽

Gram Positive Bacteria ◽

Bacterial Growth Inhibition ◽

And Staphylococcus Aureus

A series of ruthenium(II) complexes with N-heterocyclic carbene (NHC) ligands of the general type (arene)(NHC)Ru(II)X2 (where X = halide) was prepared, characterized, and evaluated as antibacterial agents in comparison to the respective metal free benzimidazolium cations. The ruthenium(II) NHC complexes generally triggered stronger bacterial growth inhibition than the metal free benzimidazolium cations. The effects were much stronger against Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) than against Gram-negative bacteria (Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa), and all complexes were inactive against the fungus Candida albicans. Moderate inhibition of bacterial thioredoxin reductase was confirmed for selected complexes, indicating that inhibition of this enzyme might be a contributing factor to the antibacterial effects.

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