High CENPM Gene Expression Predict Poor Survival Outcome in Lung Adenocarcinoma
Abstract Introduction: Lung adenocarcinoma is a disease with high morbidity and mortality. The aim of our study was to investigate the relationship between the gene expression of centromere protein M (CENPM) and its prognostic impact in lung adenocarcinoma.Method: By analyzing the data of lung adenocarcinoma in database, the CENPM gene expression in lung adenocarcinoma and its relationship with clinical stage and survival time were analyzed using datasets from The Cancer Genome Atlas (TCGA)and Gene Expression Omnibus (GEO) datasets. Genes associated with CENPM expression were analyzed and subjected to functional and pathway enrichment analysis. Finally, the genetic results and treatment and survival outcomes of 20 patients with lung adenocarcinoma from our hospital were analyzed.Result: CENPM transcripts were found to be highly expressed in lung adenocarcinoma as compared with normal tissues (3.628 VS. 2.227, P < 0.001). CENPM expression was positively associated with tumor stage (3.803 vs. 3.444, p < 0.001) and nodal stage (3.992 vs. 3.573, p < 0.001). Patients with low CENPM expression achieved better progression-free survival (45.9 months vs. 25.7months, p < 0.001) and overall survival (57.5 months vs. 47.5 months, p=0.001). The CENPM expression was negatively correlated with the infiltration of most immune cells in lung adenocarcinoma tissues and positively correlated with PD1 (r = 0.231, p < 0.001) and PD-L1 (r = 0.116, p < 0.007). CENPM-related genes were enriched in the set of genes with poor prognosis as well as the set of cell cycle-related genes in lung adenocarcinoma. CENPM expression was also negatively correlated with T lymphocyte and B lymphocyte signaling pathways. Finally, CENPM-related genes were related in Rho GTPases and ATR signaling pathways.Conclusion: Our findings demonstrate that CENPM gene is highly expressed and is associated with poor prognosis in lung adenocarcinoma.