scholarly journals Universal Fragment of mtDNA for Cervidae and Other Ungulate Species Identification

2020 ◽  
Author(s):  
Ewa Filip ◽  
Tomasz Strzała ◽  
Edyta Stępień ◽  
Klaudia Pizoń
Keyword(s):  
Species Identification ◽  
Cervus Elaphus ◽  
Animal Species ◽  
Capreolus Capreolus ◽  
Identification Procedure ◽  
Free Living ◽  
Degraded Samples ◽  
Mitochondrial Cytochrome B ◽  
Routine Identification

Abstract ObjectiveThe abundance of literature and many studies aimed at the identification of free-living animal species has helped to identify specific nucleotide marker sequences. In many cases, the best marker to distinguish species is the mitochondrial genome (mtDNA) or one of its fragments. In molecular analyses of Cervidae, biological material such as blood or muscle is easily obtained, as nearly all of these species are gaming animals.ResultsIn our research, we present the case study of successful species identification based on degraded samples of bone, with the use of short mtDNA fragments. We obtained a partial sequence of the mitochondrial cytochrome b (Cytb) gene for Capreolus capreolus, Dama dama, and Cervus elaphus, that can be used for species affiliation. The proposed methodology is helpful as a routine identification procedure for a variety of tissue sources, even in cases where the samples are degraded. The new sequences have been deposited in GenBank, enriching the existing Cervidae mtDNA base.

scholarly journals A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Feng Guan ◽  
Yu-Ting Jin ◽  
Jin Zhao ◽  
Ai-Chun Xu ◽  
Yuan-Yuan Luo
Keyword(s):  
Species Identification ◽  
Animal Species ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Universal Primers ◽  
Universal Primer ◽  
Routine Identification ◽  
Pcr Rflp ◽  
Fast Processing ◽  
Rflp Assay

There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species. We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers. PCR amplifications yielded 760 bp, 737 bp, 537 bp, 486 bp, 481 bp, 464 bp, 429 bp, and 359 bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively. This primer pair had no cross-reaction with other common domestic animals and fish. The limit of detection varied from 0.01 to 0.05 ng of genomic DNA for eight animal species in a 20 µl PCR mixture. Each PCR product could be further digested into fragments with variable sizes and qualitative analysis by SspI restriction enzyme. This developed PCR-RFLP assay was sufficient to distinguish all targeted species. Compared with the previous published related methods, this approach is simple, with high throughput, fast processing rates, and more cost-effective for routine identification of meat in foodstuffs.

scholarly journals Professional Competence of Student Teachers to Implement Species Identification in Schools – A Case Study from Germany

2017 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
Petra Lindemann-Mathies ◽  
Martin Remmele ◽  
Eija Yli-Panula
Keyword(s):  
Student Teachers ◽  
Species Identification ◽  
Animal Species ◽  
Professional Competence ◽  
The Self ◽  
Experience Based Learning ◽  
Local Plant ◽  
Living Organisms

This study investigates how well prepared student teachers are to implement species identification in school. Data were collected with the help of a questionnaire and a PowerPoint presentation in which local plant and animal species were presented. Participants (n = 357) correctly identified, on average, 23% of the plants and 44% of the animals. They identified plants mainly by flower characteristics and leaves, and animals mainly by shape and colour. Family and school were key sources of participants’ knowledge of species. The self-estimated competence of participants to identify species was positively correlated with their taxonomic knowledge and the amount of time they had spent on species identification during their own schooldays. The number of correctly identified plant and animal species increased with interest in identifying species and participation in species identification courses. Participants considered learner-centred education and experience-based learning, and the use of living organisms to be most important when identifying species in school. 

scholarly journals Linking Conservation, Community Knowledge, and Adaptation to Extreme Climatic Events: A Case Study in Gorongosa National Park, Mozambique

2021 ◽  
Vol 13 (11) ◽  
pp. 6478
Author(s):  
Amemarlita Matos ◽  
Laura Barraza ◽  
Isabel Ruiz-Mallén
Keyword(s):  
National Park ◽  
Animal Species ◽  
Human Life ◽  
Management Strategies ◽  
Forest Products ◽  
Buffer Zone ◽  
Structured Interviews ◽  
Extreme Climatic Events ◽  
Water Provision

This study is based on ethnographic research that analyzes how traditional knowledge and local beliefs on biodiversity conservation relates to the local ability to adapt and be resilient to climatic changes in two communities around Gorongosa National Park, Mozambique: Nhanfisse in the buffer zone and Muanandimae in the core area. A total of 78 semi-structured interviews with heads of households were conducted. We found that both communities carried out practices and held beliefs associated with conservation, such as protecting trees and animal species considered sacred or perceived as beneficial for human life in terms of water provision and agricultural production. In addition to traditional ceremonies that respond to extreme climatic events such as drought and flood, other adaptation strategies used by the communities include moving to neighboring areas in search of better living conditions and using forest products in times of scarcity. We discuss that the management of the park should be agreed on, in a shared way, between local communities and conservation agents to ensure that these areas continue to perform the ecological, subsistence, and spiritual functions required. Our research results contribute to a better understanding of local adaptation dynamics towards extreme climatic events and improvement of management strategies.

scholarly journals Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (Muntiacus reevesi micrurus)

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 426
Author(s):  
Yun-Hsiu Hsu ◽  
Wei-Cheng Yang ◽  
Kun-Wei Chan
Keyword(s):  
Species Identification ◽  
Operating Time ◽  
Meat Products ◽  
Reaction System ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Food Allergies ◽  
Mitochondrial Cytochrome B ◽  
Meat Species ◽  
A New Technique

The identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using recombinase polymerase amplification (RPA) in combination with a lateral flow (LF) strip. The DNA extracted from a piece of Formosan Reeves’ muntjac meat was amplified by a pair of specific primers based on its mitochondrial cytochrome b gene for 10 min at a constant temperature ranging from 30 to 45 °C using RPA. Using the specific probe added to the RPA reaction system, the amplified products were visualized on the LF strip within 5 min. The total operating time from quick DNA extraction to visualizing the result was approximately 30 min. The RPA-LF system we designed was efficient when using boiled, pan-fried, roasted, stir-fried, or stewed samples. The advantages of simple operation, speediness, and cost-effectiveness make our RPA-LF method a promising molecular detection tool for meat species identification of either raw or variously cooked Formosan Reeves’ muntjac meat. It is also possible to apply this method to identify the meat of other wildlife sources.

scholarly journals Porcine Circovirus 3a Field Strains in Free-Living Wild Boars in Paraná State, Brazil

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1634
Author(s):  
Tatiana Carolina Gomes Dutra de Souza ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Raquel Arruda Leme ◽  
Gisele da Silva Porto ◽  
...  
Keyword(s):  
Animal Species ◽  
Porcine Circovirus ◽  
Whole Genome ◽  
Living Animal ◽  
Pcr Assay ◽  
Serum Samples ◽  
Viral Dna ◽  
Free Living ◽  
Wild Boars ◽  
The World

Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-3 genome. The three full-length sequences obtained were grouped in PCV-3 clade “a”, and the sequences exhibited 100% nucleotide similarity among them. The PCV-3 field strains of this study showed nucleotide and amino acid similarities of 98.5–99.8% and 98.8–100%, respectively, with whole-genome PCV-3 sequences from around the world.

Characterization of Rhodococcus equi isolates from submaxillary lymph nodes of wild boars (Sus scrofa), red deer (Cervus elaphus) and roe deer (Capreolus capreolus)

2014 ◽  
Vol 172 (1-2) ◽  
pp. 272-278 ◽  
Author(s):  
Magdalena Rzewuska ◽  
Lucjan Witkowski ◽  
Agata A. Cisek ◽  
Ilona Stefańska ◽  
Dorota Chrobak ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Cervus Elaphus ◽  
Sus Scrofa ◽  
Red Deer ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Rhodococcus Equi ◽  
Wild Boars

Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)

2014 ◽  
Vol 17 (2) ◽  
pp. 315-319 ◽  
Author(s):  
A. Bancerz-Kisiel ◽  
A. Szczerba-Turek ◽  
A. Platt-Samoraj ◽  
P. Socha ◽  
W. Szweda
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Hunting Season ◽  
Virulence Markers ◽  
Serotype O ◽  
Gene Coding ◽  
Environmental Reservoir ◽  
Enterocolitica Infection

AbstractFree-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans

scholarly journals Do Hormones, Telomere Lengths, and Oxidative Stress form an Integrated Phenotype? A Case Study in Free-Living Tree Swallows

2016 ◽  
Vol 56 (2) ◽  
pp. 138-145 ◽  
Author(s):  
J. Q. Ouyang ◽  
Á. Z. Lendvai ◽  
I. T. Moore ◽  
F. Bonier ◽  
M. F. Haussmann
Keyword(s):  
Oxidative Stress ◽  
Tree Swallows ◽  
Free Living ◽  
Living Tree ◽  
And Oxidative Stress

scholarly journals Ethnomedicinal Knowledge of Kisan Community - A Case Study

2020 ◽  
pp. 16-23
Author(s):  
Manisha KC ◽  
Nanda Bahadur Singh
Keyword(s):  
Indigenous Knowledge ◽  
Plant Species ◽  
Common Cold ◽  
Animal Species ◽  
Traditional Practices ◽  
Medicinal Purpose ◽  
Group Discussions ◽  
Key Informants ◽  
Ethnomedicinal Knowledge

In the context of depleting indigenous knowledge and their values in the Kisan Community, they seem unaware of their culturally rich traditional practices of using animals and plants for medicinal purpose. It has been essential to document those knowledge of practices for future references. The paper tried to explore and document those knowledge and practices by the Kisan community of Mechinagar 9 and 11 of Jhapa, Nepal. For this purpose, the data were collected through interviews with key informants including conjurer (Dhami/Jhakri), elders of the society and also focal group discussions with the local people. Analysis of the data has shown that 29 animal species belonging to 24 families are used to treat 29 ailments and 57 plant species belonging to 37 families are used for treating 39 different ailments. The commonly treated ailments were common cold, cuts, wounds, diarrhoea, dysentery, etc.

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