Analysis of Oral Microbial Dysbiosis Associated With Early Childhood Caries

Author(s):  
Hui Zheng ◽  
Teng-Fei Xie ◽  
Shao-Kai Li ◽  
Xiao-Tong Qiao ◽  
Yan Feng ◽  
...  

Abstract Background: "Core microbes" play a key role in the development of caries and lead to microflora disorders. Our goal was to detect the core microbes associated with the microbial flora imbalance in early childhood caries (ECC). Methods: Fifteen caries-free children and fifteen high-caries (DMFT≥10) children aged 4-6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from plaque samples loaded in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.Results: There were statistically significant differences between the groups regarding the relative abundance of S. mutans (Wilcoxon rank-sum test, P<0.05). No significant difference was found between the groups regarding other species or functional genes. Conclusion: Neither unique microbes leading to early caries in deciduous teeth nor characteristics of a microbial community with a disordered structure were found. Some microorganisms related to dental caries in young children were found, but whether these microbes were truly related or were false positives requires further verification.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hui Zheng ◽  
Tengfei Xie ◽  
Shaokai Li ◽  
Xiaotong Qiao ◽  
Youguang Lu ◽  
...  

Abstract Background "Core microbes" play a key role in the development of caries and lead to microbial disorders. Our goal was to detect the core microbes associated with the microbiota imbalance in early childhood caries (ECC). Methods Fifteen caries-free children and fifteen high-caries (DMFT ≥ 10) children aged 4–6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from samples of plaque in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms. Results There were statistically significant differences between the groups regarding the relative abundance of Streptococcus mutans (Wilcoxon rank-sum test, P < 0.05). No significant difference was found between the groups regarding other species or functional genes. Conclusion S. mutans, together with other pathogens, may play a prominent role and act as "core microbes" in the occurrence and development of early childhood caries.


2020 ◽  
Vol 981 ◽  
pp. 271-279
Author(s):  
Muhammad Zaki Ramli ◽  
Nina Suhaity Azmi

Oral caries remains a significant public health problem and affects children with early childhood caries (ECC) and severe early childhood caries (S-ECC). However, the mechanisms of these complex multi-species communities are not well understood. This article systematically reviews the studies involving children with caries to learn the demographics of the subjects, their subjects and types of sample, the methodological approaches undertaken, the main findings, and eventually to make recommendations for future research. A search using a designated search string in the Web of Science resulted in 148 articles. A careful examination of each article based on the inclusion criteria resulted in a final 40 articles. Most of the studies were conducted in the United States and China. The studies involved mostly, but not exclusively, children with primary dentition and were having caries. Different types of subjects were sampled from different racial backgrounds and diseases. Different samples were collected, such as supragingival plaque, saliva and biofilm. Clonal analyses and anaerobic approaches were used to characterise the oral microbiome, including shotgun sequencing, pyrosequencing of 16S rRNA genes, and Pacbio sequencing. Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria were among the phyla commonly reported by the studies. Streptococcus mutans was a key aetiologic agent in the development of caries, besides Veillonella, Prevotella spp. and Lactobacillus spp. Some studies developed models and discussed the applications of oral microbiome in relation to diseases. We recommend further reviews on studies strictly on children with caries and studies involving children only for a better generalisation of the findings. More studies relating oral microbiome with dietary intake and other diseases can also be conducted.


2021 ◽  
Vol 162 (22) ◽  
pp. 861-869
Author(s):  
Andrea Radácsi ◽  
Tímea Dergez ◽  
Laura Csabai ◽  
Nóra Stáczer ◽  
Krisztián Katona ◽  
...  

Összefoglaló. Bevezetés és célkitűzés: A 3 év alatti korcsoportban a súlyos kisgyermekkori fogszuvasodás (S-ECC) gyakoriságának vizsgálata és a kisgyermekes szülők szájegészséggel kapcsolatos ismereteinek felmérése. Módszer: Háromszázhatvankét, 36 hónapos vagy annál fiatalabb gyermek (átlagéletkor: 28,49 ± 5,25 hónap) fogászati szűrővizsgálatát végeztük el, és szüleik számára önkéntesen kitölthető kérdőívet állítottunk össze. A 306 kitöltött kérdőív eredményét a gyermekek fogászati statusával egyénenként összevetve statisztikai analízist végeztünk. Eredmények: A gyermekek fogászati szűrésekor 15,46% volt a cariesprevalencia: df-t-index = 0,685 ± 2,20; az általunk javasolt, az előtört fogak számával módosított df-t-index = 0,758 ± 2,42, SiC-index = 2,06 ± 3,33. A vizsgált populációban nem találtunk tömött vagy fogszuvasodás miatt eltávolított fogat. A korábban szájhigiénés tájékoztatásban részesült/nem részesült szülők gyermekeinél nem volt szignifikáns különbség a szuvas fogak számának (p = 0,196), a fogyasztott folyadék cukortartalmának (81,5%/71,5%) és a bevitel módjának (p = 0,453) tekintetében. A gyermeküket 6 hónapos korukig kizárólagosan anyatejjel tápláló édesanyák nagyobb eséllyel választották a vízzel történő itatást (75%/52%) pohárból (68,1%/28,8%) a későbbiekben. Az édesanya legmagasabb iskolai végzettsége kulcsszerepet játszik mind az anyatejes táplálás melletti elhivatottságban (felsőfok: 53,4%, középfok: 34,2%, alapfok: 37,5%), mind a cariesfrekvencia csökkentésében (p = 0,015). Következtetés: A szülői szájhigiénés prevenciós tájékoztatás jelenleg nem hatékony. Eredményeink alapján a fogászati szűrést 1 éves kor előtt meg kell kezdeni, amely a hatékony, rendszeres fogászati prevenciós tanácsadás lehetőségét is megteremtené. Szükségesnek látjuk a védőnők és a gyermekháziorvosok folyamatos továbbképzésében a kisgyermekkori fogszuvasodás alapismereteinek integrációját. Orv Hetil. 2021; 162(22): 861–869. Summary. Introduction and objective: To investigate the frequency of severe early childhood caries (S-ECC) under 3 years of age and to assess the oral health related knowledge of parents/guardians with preschool children. Method: 362 children younger than 36 months (mean age: 28.49 ± 5.25 months) were screened and a voluntary questionnaire for their parents was compiled. Statistical analysis was carried out comparing the results of the 306 completed questionnaires with the dental status of the screened children. Results: Caries prevalence in the examined population was 15.46%, df-t index = 0.685 ± 2.20, our suggested modified df-t index based on the number of erupted teeth = 0.758 ± 2.42, SiC-index = 2.06 ± 3.33. No filled or extracted tooth due to caries was found in the study group. There was no significant difference in the number of carious teeth (p = 0.196), consumption of sugar-containing drinks (81.5%/71.5%) and administration of drinks (p = 0.453) in the case of children whose parents had previously received/not received oral hygiene information. Mothers who exclusively breastfed until the age of 6 months were more likely to choose to offer water (75%/52%) from cup (68.1%/28.8%) later. The mother’s highest level of education plays a key role in both the commitment to breastfeeding (tertiary: 53.4%, secondary: 34.2%, primary: 37.5%) and in reducing the incidence of caries (p = 0.015). Conclusion: Parental oral hygiene preventive instruction is currently ineffective. Based on our results, dental screening should be started before the age of 1 year, that would also provide an opportunity of effective, regular dental prevention counseling. There is a need for the integration of the basics of early childhood caries in the continuous professional training of district nurses and pediatricians. Orv Hetil. 2021; 162(22): 861–869.


Author(s):  
AM Castro ◽  
CGC Reis ◽  
JGF Costa ◽  
RM Alcântara ◽  
DCA Ferreira ◽  
...  

2019 ◽  
Vol 13 (1) ◽  
pp. 242-248
Author(s):  
Jenny E. C. Acuña ◽  
Karina M. S. Freitas ◽  
Rafael P. Henriques ◽  
Emerson F. Cruz ◽  
Maria C. R Binz Ordóñez ◽  
...  

Background: Early childhood caries is an aggressive pathology that can destroy the teeth in a short time, reaching the proximal surfaces, causing discomfort, pain and affecting the dental pulp, causing premature loss of deciduous teeth. Objective: The purpose of this research is to determine the prevalence of early childhood caries in children aged 1 to 5 years of the Metropolitan District of Quito. Materials and Methods: This is a cross-sectional study including a sample of 557 children attending child care centers in Quito, Ecuador, between May and July 2018. The sample was evaluated clinically registering the data in a simplified dental file containing the odontogram and the result of decayed, lost and sealed teeth, according to the dmft index, as well as data such as age and gender. Data were tabulated and analyzed statistically by independent t-test. Results: The prevalence of early childhood caries was found to be 59.61%. Children affected by early childhood caries presented a mean age of 2.83 years, the ones suffered the most from this pathology were those of 2 years (35.54%), followed by those of 3 years (34.94%) and 4 years (22.5%). Boys presented more early childhood caries (53.92%) in relation to the girls (46.08%). Conclusion: The early childhood caries presented a prevalence of 59.61% and a general dmft index of medium level of severity according to the classification of the World Health Organization, in children from 1 to 5 years of age in Quito, Ecuador.


Author(s):  
Dhanalakshmi Ravikumar ◽  
Pratibha Ramani ◽  
R. Gayathri

Background and Objectives: Early childhood caries is a major public health problem especially in young children. ECC affects the quality of life of young children by affecting the chewing ability of children due to the development of pain and swelling, and this may indirectly affect the nutritional status of a child. The present study was done to assess the level of salivary malondialdehyde in children with and without Early childhood caries. The main objective of the study is to determine the salivary malondialdehyde levels and to compare it with the three groups of children with different caries experiences. Materials and Methods: It is cross-sectional observational research carried out at Saveetha Dental College and Hospitals. Children between 3-6 years were examined and 20 caries-free, 20 children with ECC as well as 20 children with S-ECC were recruited for the study. The caries status was assessed using dmfs and the severity of caries was assessed using pufa index. Salivary samples were collected and analysed for salivary malondialdehyde levels. Statistical Analysis: The data was entered and analysed using SPSS software version 20.0. One-way ANOVA was done to determine the difference in malondialdehyde levels between the groups. “Post-hoc Tukey” test was done to measure the intergroup significance. A “P value of < 0.05” was measured as statistically “significant” and P < 0.001 was deemed as statistically “highly significant”. Pearson’s correlation was done to correlate pufa values with malondialdehyde levels. Results: The results demonstrated that there was a statistically significant difference in salivary malondialdehyde levels among caries-free, ECC as well as S-ECC children. There was a positive correlation between the pufa score and salivary malondialdehyde levels. Conclusion: 1. There is a significant difference in the salivary malondialdehyde levels among caries-free, ECC as well as S-ECC children. There was a positive correlation of salivary malondialdehyde levels and pufa score in ECC and S-ECC children.


2020 ◽  
Vol 8 (1) ◽  
pp. 33-41
Author(s):  
Zhafirah Muharani Nasution ◽  
Reni Nofika ◽  
Susi Susi

Dental caries is a disease of hard tissue of the tooth due to bacterial activities that cause loss mineral structure of the tooth. Dental caries that occur in deciduous teeth on children under 71 months is called Early Childhood Caries (ECC). Dental caries is still one of the most common dental and oral health problem experienced by children in the world. Some surveys in Cambodia and Indonesia show that a high prevalence and severity of ECC which was 90% in children aged 3-5 years. Oral hygiene habits were identified as one of the risk factors of caries. Some studies suggest that there is a strong relationship between the behavior of maintaining oral hygiene and the prevalence of dental caries. The purpose of this study is to determine the relationship between oral hygiene habits and early childhood caries on toddler in Padang. This study used cross sectional design. The samples of this study was 107 childrens aged 3-5 years in Padang. The location was selected through stratified random sampling technique and samples was selected through consecutive sampling techniques. Data was collected in Gunung Pangilun village and Piai Tanah Sirah village. Data was collected using a questionaires and intraoral examination. Data was analyzed by Chi-Square with p<0,05. The result of this study that there was a significant relationship between oral hygiene habits and ECC on toddler in Padang (p=0,008). The conclusion of this study was that oral hygiene habits is associated with early childhood caries on toddler in Padang.


New Medicine ◽  
2019 ◽  
Vol 23 (4) ◽  
Author(s):  
Angelika Kobylińska ◽  
Dorota Olczak-Kowalczyk

Introduction. Odontogenesis of deciduous teeth is dependent on the intrauterine environment and shaped, among other things, by prenatal maternal nutrition. Proper deve-lopment of deciduous dentition, formation and mineralisation of the enamel and dentin determines the susceptibility to cariogenic factors. The current knowledge of the effects of prenatal nutrition and supplementation on the risk of early childhood caries (ECC) is unclear. Aim. An evaluation of the relationship between maternal nutrition in pregnancy and the risk of early childhood caries (ECC). Material and methods. A systematic review of literature on the association between prenatal maternal nutrition and early childhood caries was performed based on both electronic and manual search through medical databases, i.e. PubMed, EMBASE and MEDLINE, which allowed to identify 6 studies meeting the inclusion criteria. The risk of bias was evaluated using the ROBINS-I questionnaire and a quality assessment of original papers was performed using the Newcastle-Ottawa Scale (NOS). Results. All analysed works were at a risk of bias associated with confounders affecting the intervention and interpretation of results. Four works were considered to be of good quality (NOS > 6) and two other of medium quality. Conclusions. An increased maternal intake of vitamin D, calcium, dairy products, yoghurts and cheeses in pregnancy is an important factor reducing the risk of dental caries in their children. The risk of ECC is increased in the case of vitamin D deficiency, low intake of calcium and a diet high in fatty acids and carbohydrates.


2019 ◽  
Vol 98 (5) ◽  
pp. 549-555 ◽  
Author(s):  
R. Singleton ◽  
G. Day ◽  
T. Thomas ◽  
R. Schroth ◽  
J. Klejka ◽  
...  

Alaska Native (AN) children experience one of the highest reported rates of severe early childhood caries (S-ECC). Serum vitamin D concentrations in AN childbearing women in the Yukon Kuskokwim Delta (YKD) region have decreased since the 1960s to currently low levels, related to a decrease in traditional marine diet. Recent studies suggest an association between prenatal vitamin D (25(OH)D) concentrations in mothers and S-ECC in their infants. We used independent t tests to analyze the influence of prenatal 25(OH)D levels in YKD AN mothers on S-ECC in their children using data collected in the Maternal Organics Monitoring Study (MOMS). Maternal 25(OH)D levels were assessed at prenatal visits and in cord blood. We queried electronic dental records to assess early childhood caries (ECC) status using highest decayed, missing, filled, primary teeth (dmft) scores at 12 to 59 mo of age. We examined prenatal and cord blood for 76 and 57 mother/infant pairs, respectively. Children 12 to 35 mo of age with “deficient” cord blood (25(OH)D <30 nmol/L) had a mean dmft score twice as high as children who were “nondeficient” at birth (9.3 vs. 4.7; P = 0.002). There was no significant difference in mean dmft scores for children aged 36 to 59 mo with deficient versus nondeficient cord blood 25(OH)D (10.9 vs. 8.7 P = 0.14). There was no significant difference in mean dmft scores for children aged 12 to 35 mo whose mothers had “sufficient” versus “insufficient” 25(OH)D during prenatal visits (9.0 vs. 7.4; P = 0.48). In this small sample, children with deficient vitamin D levels in cord blood had a dmft score at 12 to 35 mo 2-fold higher than children with nondeficient cord blood. Maternal 25(OH)D may influence the primary dentition, and improving vitamin D status in pregnant women might affect ECC rates in their infants.


2021 ◽  
Vol 12 ◽  
Author(s):  
Vivianne Cruz de Jesus ◽  
Mohd Wasif Khan ◽  
Betty-Anne Mittermuller ◽  
Kangmin Duan ◽  
Pingzhao Hu ◽  
...  

The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) &lt; 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal microbiomes. Differential abundance analyses showed that, among others, the cariogenic species Streptococcus mutans was enriched in the dental plaque, compared to oral swabs, of children with S-ECC. The fungal species Candida dubliniensis and C. tropicalis were more abundant in the oral swab samples of children with S-ECC compared to caries-free controls. They were also among the top 20 most important features for the classification of S-ECC vs. caries-free in oral swabs and for the classification of dental plaque vs. oral swab in the S-ECC group. ML approaches revealed the possibility of classifying samples according to both caries status and sampling sites. The tested site of sample collection did not change the predictability of the disease. However, the species considered to be important for the classification of disease in each sampling site were slightly different. Being able to determine the origin of the samples could be very useful during the design of oral microbiome studies. This study provides important insights into the differences between the dental plaque and oral swab bacteriome and mycobiome of children with S-ECC and those caries-free.


Sign in / Sign up

Export Citation Format

Share Document