scholarly journals Multifunctional MOF-based Electrochemiluminescent Nanocubes for an Ultrasensitive Biosensing Strategy of B. Pseudomallei Determination Coupled With 3D Magnetic Walking Nanomachine

2021 ◽  
Author(s):  
Yuexin Wang ◽  
Bo Shen ◽  
Cai Li ◽  
Haiping Wu ◽  
Yanshuang Wang ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Burkholderia Pseudomallei ◽  
Detection Method ◽  
Accurate Determination ◽  
Serum Samples ◽  
Exonuclease Iii ◽  
Specific Performance ◽  
Versatile Tool ◽  
Exo Iii

Abstract Burkholderia pseudomallei (B. pseudomallei) can cause melioidosis that is usually fatal. A reliable and rapid detection method is greatly needed for disease surveillance and diagnosis. Herein, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed for accurate determination of B. pseudomallei coupled with multifunctional Au@Co-MOF@ABEI nanocubes and 3D magnetic walking nanomachine. The synthesized nanocubes could not only immobilize enormous ABEI but exhibit superior peroxidase-like activity to decompose H2O2 to produce plentiful reactive oxygen species (ROSs) that could further react with ABEI, so that the enhanced ECL signals were achieved. Meanwhile, the target-activated walking nanomachine was efficiently driven by Exonuclease III (Exo III) for further improving the sensitivity of the biosensor. As a result, the fabricated ECL biosensor could detect pathogenic gene down to 60.3 aM with a linear range from 100.0 aM to 100.0 pM. Moreover, the biosensing platform successfully achieved the determination of B. pseudomallei down to 9.0 CFU mL−1 in serum samples. This work exhibited an ultrasensitive and specific performance for B. pseudomallei detection, which would become a versatile tool in the early diagnosis and treatment of melioidosis.

scholarly journals Effects of Delayed Sample Processing on Determination of Total and High Molecular Weight (HMW) Adiponectin in Serum and Plasma: A Pilot Study

2016 ◽  
Vol 8 (3) ◽  
pp. 19
Author(s):  
Choaping Ng ◽  
Felicity J Rose ◽  
Sahar Keshvari ◽  
Marina M Reeves ◽  
Goce Dimeski ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Pilot Study ◽  
High Molecular Weight ◽  
Accurate Determination ◽  
Mean Difference ◽  
Serum Samples ◽  
Blood Samples ◽  
Hmw Adiponectin ◽  
Total Adiponectin

<p>Adiponectin is a beneficial adipocyte-secreted hormone, which circulates in a variety of multimeric forms termed low and high molecular weight (LMW/HMW). Effectiveness of clinical therapeutic trials which target adiponectin rely on accurate determination of circulating total and HMW adiponectin levels but the accuracy may be influenced by variations in sample handling processes. The aim of this pilot study was to investigate the effects of delayed processing of blood samples on the concentration of total and HMW adiponectin.</p><p>Materials and Methods: Fasting blood samples were collected for analysis of total and HMW adiponectin concentrations in EDTA plasma and serum from eight healthy participants.  Samples were centrifuged post 15 min storage at 4<sup>o</sup>C as the comparative ‘ideal’ method or after up to 72 h of refrigerated storage or 6 h at room temperature. Total and HMW adiponectin concentrations were measured by ELISA.</p><p>Results: Under ideal handling conditions measurements of total and HMW adiponectin concentrations were significantly higher in serum than in plasma (mean difference: -1.3 µg/mL [95% CI: -1.6, -1.0], p&lt;0.001; and, -0.6 µg/mL [95% CI: -0.7, -0.5], p&lt;0.001, respectively).  Storage of blood samples at 4<sup>o</sup>C for 72 h resulted in significant reductions in concentration of total adiponectin in serum (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) and HMW adiponectin in plasma (mean difference: -0.6 µg/mL [95%CI: -0.9, -0.2], p=0.007), compared with ideal conditions.  Further analysis of serum samples showed a significant decrease in total adiponectin concentration after 6 h storage at 4<sup>o</sup>C (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) compared with ideal conditions.</p><p>Conclusions: Delayed processing of samples may have differential effects on the concentration of total and HMW adiponectin in serum or plasma. Larger studies are warranted for clinical intervention trials.</p>

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent

RSC Advances ◽  
2016 ◽  
Vol 6 (69) ◽  
pp. 64895-64901 ◽  
Author(s):  
Fengli Qu ◽  
Lian Xia ◽  
Chuanxiang Wu ◽  
Lijie Liu ◽  
Guoliang Li ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Accurate Determination ◽  
Extraction Procedure ◽  
Sialic Acids ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Serum Samples ◽  
Solid Phase Extraction Procedure

An zirconium-based MOFs of UiO-66-NH2 has been synthetized and characterized in a dispersive solid-phase extraction procedure combined with HPLC with fluorescence detection for the pre-concentration and detection of sialic acids in serum samples.

Effect of urine storage on urinary uric acid concentrations

2000 ◽  
Vol 37 (3) ◽  
pp. 355-359 ◽  
Author(s):  
Jun-Ichi Yamakita ◽  
Tetsuya Yamamoto ◽  
Yuji Moriwaki ◽  
Sumio Takahashi ◽  
Zenta Tsutsumi ◽  
...  
Keyword(s):  
Uric Acid ◽  
Acid Concentration ◽  
Storage Temperature ◽  
Accurate Determination ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Diagnosis And Classification ◽  
Urine Storage

Accurate determination of serum and urinary uric acid concentrations is essential for the diagnosis and classification of gout according to uric acid metabolism derangement. Urine and/or serum samples are often kept at either 4°C or 20°C until assayed, when a large number of samples are handled simultaneously. Our preliminary study indicated a significant decrease in urinary uric acid concentration after preservation, regardless of the storage temperature. Uric acid crystals were often observed in these cases which showed a marked decrease in urinary uric acid concentration after storage. In the present study, we sought the factor(s) that might cause this decrease in urinary uric acid concentration, as well as measures to overcome the problem. High urinary uric acid concentration and low pH proved to play major roles in the decrease in urinary uric acid concentration after storage. In contrast, dilution of the urine samples before storage resulted in no significant change in urinary uric acid concentration. Based on these results, we recommend diluting urine before storage for determination of uric acid concentration and avoiding underestimation.

scholarly journals Determination of glyphosate and glufosinate in human serum by monospin TiO extraction and liquid chromatography–tandem mass spectrometry

2020 ◽  
Vol 32 (1) ◽  
pp. 10-15
Author(s):  
Takeshi Saito ◽  
Akira Namera ◽  
Tomoatsu Tsuji ◽  
Wataru Noguchi ◽  
Sadaki Inokuchi
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Serum ◽  
Accurate Determination ◽  
Tandem Mass ◽  
Serum Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

We developed and validated an assay for determination of glyphosate (GLYP) and glufosinate (GLUF) in human serum. Serum samples were extracted by using a MonoSpin® TiO column and analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). MonoSpin® TiO tends to specifically bind to phosphate groups. The assay was linear over a concentration range of 1–250 μg/mL. The recoveries for the 2 compounds were 1.6%–2.3%. The intra- and inter-day variations were <15%. Precision and accuracy were 5.6%–12.7% and 97.0%–103.9%, respectively. The validated method was applied to quantify the GLYP and GLUF content in the serum of GLYP and GLUF-poisoned patients. In conclusion, the method was successfully applied for accurate determination of GLYP and GLUF in serum obtained from patients with GLYP and GLUF poisoning.

scholarly journals Evaluation of four sampling devices for Burkholderia pseudomallei laboratory aerosol studies

2021 ◽  
Vol 15 (2) ◽  
pp. e0009001
Author(s):  
Michael Schuit ◽  
Sierra Gardner ◽  
Jill Taylor ◽  
Paul Dabisch
Keyword(s):  
Animal Models ◽  
Burkholderia Pseudomallei ◽  
Culture Media ◽  
Accurate Determination ◽  
Field Studies ◽  
Cascade Impactor ◽  
Laboratory Studies ◽  
Air Samples ◽  
Sampling Process

Previous field and laboratory studies investigating airborne Burkholderia pseudomallei have used a variety of different aerosol samplers to detect and quantify concentrations of the bacteria in aerosols. However, the performance of aerosol samplers can vary in their ability to preserve the viability of collected microorganisms, depending on the resistance of the organisms to impaction, desiccation, or other stresses associated with the sampling process. Consequently, sampler selection is critical to maximizing the probability of detecting viable microorganisms in collected air samples in field studies and for accurate determination of aerosol concentrations in laboratory studies. To inform such decisions, the present study assessed the performance of four laboratory aerosol samplers, specifically the all-glass impinger (AGI), gelatin filter, midget impinger, and Mercer cascade impactor, for collecting aerosols containing B. pseudomallei generated from suspensions in two types of culture media. The results suggest that the relative performance of the sampling devices is dependent on the suspension medium utilized for aerosolization. Performance across the four samplers was similar for aerosols generated from suspensions supplemented with 4% glycerol. However, for aerosols generated from suspensions without glycerol, use of the filter sampler or an impactor resulted in significantly lower estimates of the viable aerosol concentration than those obtained with either the AGI or midget impinger. These results demonstrate that sampler selection has the potential to affect estimation of doses in inhalational animal models of melioidosis, as well as the likelihood of detection of viable B. pseudomallei in the environment, and will be useful to inform design of future laboratory and field studies.

A simple and rapid dual-cycle amplification strategy for microRNA based on graphene oxide and exonuclease III-assisted fluorescence recovery

2018 ◽  
Vol 10 (30) ◽  
pp. 3777-3782 ◽  
Author(s):  
Yafang Tang ◽  
Mingxiu Liu ◽  
Lingcao Xu ◽  
Jianniao Tian ◽  
Xiulin Yang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Detection Method ◽  
Fluorescence Recovery ◽  
Exonuclease Iii ◽  
Microrna Detection ◽  
Dual Cycle ◽  
Exo Iii ◽  
Amplification Strategy

A simple microRNA detection method by combining Graphene Oxide (GO) fluorescence quenching with exonuclease III (Exo-III) aided cycling amplification was developed.

Oxidation Markedly Reduces Bilirubin Interference in the Jaffé Creatinine Assay

1992 ◽  
Vol 38 (12) ◽  
pp. 2411-2413 ◽  
Author(s):  
G Rajs ◽  
M Mayer
Keyword(s):  
Serum Creatinine ◽  
Accurate Determination ◽  
Serum Samples ◽  
Average Recovery ◽  
Subsequent Oxidation

Abstract Bilirubin causes underestimation of serum creatinine in the Jaffé alkaline picrate assay. We report an approach for preventing bilirubin interference by pretreating serum samples with peroxidase and H2O2. The dissociation of bilirubin from albumin and its subsequent oxidation markedly reduces the bilirubin interference and enables accurate determination of creatinine concentrations by the Jaffé reaction even in hyperbilirubinemic sera. Within-run CVs were 2.6%, 4.0%, and 3.8% at mean creatinine concentrations of 88, 165, and 349 mumol/L, respectively (n = 20). Day-to-day CVs were 4.0%, 6.3%, and 5.8% for mean creatinine concentrations of 87, 168, and 364 mumol/L, respectively (n = 12). Average recovery of creatinine added to serum in the presence of 600 mumol/L bilirubin was 97% (n = 15). This method requires only small serum volumes (70 microL) and is easily applicable to automated analyzers that can be programmed to add three reagents consecutively.

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