scholarly journals Evaluation of a novel and rapid screening method for the detection of contaminated colistin-resistant bacteria in food

2020 ◽  
Author(s):  
Hanh Vu ◽  
Cornelia Appiah-Kwarteng ◽  
Kaori Tanaka ◽  
Ryuji Kawahara ◽  
Diep Thi Khong ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Method ◽  
High Speed ◽  
Screening Method ◽  
Rapid Screening ◽  
Resistant Bacteria ◽  
Practical Utility ◽  
E Coli ◽  
Retail Meat ◽  
Meat Samples

Abstract Background: The dissemination of colistin-resistant bacteria carrying the colistin-resistant mobile gene, mcr-1 threatens medical care worldwide. In particular, contamination of food with colistin-resistant bacteria accelerates the community dissemination of colistin-resistant bacteria. Therefore, monitoring of colistin-resistant bacteria in food is important for controlling resistant bacteria. Unfortunately, the conventional culture methods for detecting colistin-resistant bacteria are not practical for monitoring food saftey. Therefore, development of a simple and rapid method to detect food contamination with colistin-resistant bacteria is desirable as an effective means for preventing the dissemination of resistant bacteria, particularly colistin-resistant bacteria.Findings: We developed a simple and rapid method for detecting Escherichia coli harboring the mcr-1 colistin resistance gene using a high-speed real-time polymerase chain reaction (PCR). The entire procedure, from sample processing to finals results, was performed within one hour. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results of the developed method were in agreement with the results of culturing colistin-resistant E. coli from the meat samples, demonstrating its efficacy and usefulness.Conclusions: A simple and rapid real-time PCR-based screening method was developed for detecting E. coli harboring mcr-1 in food samples. The practical utility of the procedure was confirmed using retail meat samples, indicating its potential as a convenient and rapid method to detect bacterial contamination of food items, especially in developing communities.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Thang N. Nguyen ◽  
Diep T. Khong ◽  
Ha V. Le ◽  
Hoa T. Tran ◽  
Quang N. Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene, mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 10 4 and 4.2 × 10 3  CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli was a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

Interlaboratory Validation of a Real-Time PCR 24-Hour Rapid Method for Detection of Salmonella in Foods

2009 ◽  
Vol 72 (5) ◽  
pp. 945-951 ◽  
Author(s):  
CHORNG-MING CHENG ◽  
KHANH T. VAN ◽  
WEN LIN ◽  
RICHARD M. RUBY
Keyword(s):  
Real Time ◽  
Screening Method ◽  
Rapid Screening ◽  
Selective Enrichment ◽  
Manual Method ◽  
Qpcr Method ◽  
Peptone Water ◽  
Interlaboratory Validation ◽  
Chili Powder ◽  
The One

The efficacy of a 24-h Salmonella real-time, or quantitative, PCR (qPCR) detection method was assessed through a collaborative effort involving eight Federal and state laboratories. Eleven foods including mashed potatoes, soft cheese, chili powder, chocolate, eggs, sprouts, apple juice, fish, shrimp, ground beef, and ground chicken were tested. For each food, seven blind samples were distributed to each participant for testing. These included six samples equivalently inoculated with 1 to 5 CFU/25 g of various serotypes of Salmonella (Gaminara, Weltevreden, Heidelberg, Senftenberg, Enteritidis, Newport, Typhimurium, and Kentucky for each food) and 10 to 50 CFU/25 g of the competitor Enterobacter cloacae. The seventh sample was inoculated with 10 to 50 CFU/25 g of the competitor, E. cloacae, only. These samples were tested for Salmonella by using four methods in parallel: (i) 24-h qPCR method detecting Salmonella from modified buffered peptone water enrichment medium; (ii) 48-h qPCR method detecting Salmonella from a secondary selective enrichment broth; (iii) modified Bacteriological Analytical Manual method; and (iv) VIDAS, an immunoassay system. The results of the statistical analysis showed there was no significant (P ≥ 0.05) difference between either of the qPCR methods and the modified Bacteriological Analytical Manual method for 10 of 11 foods. For the one exception, sprouts, detection by qPCR required 48 h. Both qPCR methods showed a detection limit of 0.08 to 0.2 CFU/g. These results provide a solid basis for using this 24-h qPCR rapid screening method to detect Salmonella in foods.

A novel rapid screening method for health monitoring of building structures from earthquake records

2019 ◽  
Vol 22 (16) ◽  
pp. 3544-3557
Author(s):  
Ging-Long Lin ◽  
Jer-Fu Wang ◽  
Chi-Chang Lin ◽  
Jim Lin
Keyword(s):  
Real Time ◽  
Health Monitoring ◽  
Screening Method ◽  
Nonlinear Behavior ◽  
Relative Displacement ◽  
Rapid Screening ◽  
Building Structures ◽  
Detection Techniques ◽  
Earthquake Records ◽  
Rapid Screening Method

This study presents a rapid screening method for health monitoring of building structures based on earthquake records. Compared with conventional damage detection techniques, the rapid screening system with few sensors is more attractive and cost-effective in assessing the global behaviors of a building structure. Only two tri-axial accelerometers are required for a building. One is mounted at the ground level, and another one is mounted at the top floor. First, the relative displacement of top floor to ground is calculated by on-line integration. Then, the diagram of absolute acceleration versus relative displacement of top floor is used to determine the pseudo stiffness of the whole building by linear regression. The decrease of pseudo stiffness denotes the occurrence and degree of damage in the building. A novel real-time damage technique is also proposed to detect nonlinear behavior of a building. A five-story shear-type building under earthquake excitations was illustrated for sensitivity analysis of pseudo stiffness considering different damage cases. Shaking-table-test data of a three-story benchmark building were used to verify the accuracy of the proposed damage assessment technique. In addition, the proposed method was also applied to evaluate a new eight-story residential building equipped with accelerometers in Taipei, Taiwan. Finally, the acceleration response records of a real building, which experienced moderate damages caused by the main shock of 1999 Taiwan Chi-Chi earthquake ( ML = 7.3), were considered to examine the applicability of the proposed method to generate a real-time damage indicator for a building with nonlinear behavior. All of the results show that the proposed method is reliable and effective for rapid diagnosis of building health.

scholarly journals Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler

2011 ◽  
Vol 77 (22) ◽  
pp. 8193-8196 ◽  
Author(s):  
Lucja M. Jarosz ◽  
Bastiaan P. Krom
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Fluorescent Protein ◽  
Screening Method ◽  
Rapid Screening ◽  
Content Type ◽  
Wide Range ◽  
Hyphal Formation ◽  
Green Fluorescent

ABSTRACTWe propose a screening method for compounds affecting growth and germination inCandida albicansusing a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using PACT1-GFPand PHWP1-GFPreporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.

scholarly journals Adaptive nanopore sequencing on miniature flow cell detects extensive antimicrobial resistence

2021 ◽  
Author(s):  
Adrian Viehweger ◽  
Mike Marquet ◽  
Martin Hölzer ◽  
Nadine Dietze ◽  
Mathias Pletz ◽  
...  
Keyword(s):  
Real Time ◽  
Hospital Admissions ◽  
Microbial Consortium ◽  
Low Cost ◽  
Flow Cell ◽  
Rapid Screening ◽  
Resistant Bacteria ◽  
Cost Constraints ◽  
Antimicrobial Resistance Genes ◽  
New Type

Rapid screening of hospital admissions to detect asymptomatic carriers of resistant bacteria can prevent pathogen outbreaks. However, the resulting isolates rarely have their genome sequenced due to cost constraints and long turn-around times to get and process the data, limiting their usefulness to the practitioner. Here we use real-time, on-device target enrichment ("adaptive") sequencing on a new type of low-cost nanopore flow cell as a highly multiplexed assay covering 1,147 antimicrobial resistance genes. Using this method, we detected four types of carbapenemase in a single isolate of Raoultella ornithinolytica (NDM, KPC, VIM, OXA). Further investigation revealed extensive horizontal gene transfer within the underlying microbial consortium, increasing the risk of resistance spreading. Real-time sequencing could thus quickly inform how to monitor this case and its surroundings.

scholarly journals Selective Uropathogenic E. coli Detection using Crossed Surface Relief Gratings

2018 ◽  
Author(s):  
Srijit Nair ◽  
Juan Gomez-Cruz ◽  
Angel Manjarrez-Hernandez ◽  
Gabriel Ascanio ◽  
Ribal Georges Sabat ◽  
...  
Keyword(s):  
Effective Treatment ◽  
Real Time ◽  
Surface Relief ◽  
Incident Light ◽  
Resistant Bacteria ◽  
Public Healthcare ◽  
E Coli ◽  
Label Free Detection ◽  
Surface Relief Gratings ◽  
Tract Infections

Given the rise in the number of cases and their recurrences, Urinary Tract Infections (UTI) are one of the major burdens on public healthcare worldwide. Rapid, inexpensive and selective detection of Uropathogenic E. coli (UPEC), a major contributor to UTIs, is the need of the hour for effective treatment, given the rise of antibiotic-resistant bacteria due to improper diagnosis. Here we present a rapid, real-time, selective and label-free detection of UPEC using an integrated sensing platform based on Crossed Surface Relief Gratings (CSRGs) as nanoplasmonic sensors. Detection is achieved due to the unique Surface Plasmon Resonance (SPR)-based light energy exchange attributed to the CSRGs, allowing real-time sensing in a very narrow bandwidth of the incident light to pass where the SPR energy conversion occurs. The sensing ability of the platform is experimentally demonstrated by the detection of bulk Refractive Index (RI) changes, with a bulk sensitivity of 382.2 nm/RIU and a resolution in the order of 10-6 RIU. We demonstrate selective capture and detection of clinical concentration of UPEC, as opposed to other gram-negative bacteria, in real-time, a first for CSRGs. This work is particularly important for effective treatment of UTIs, allowing point-of-care diagnosis for economically disadvantaged regions around the world.

Rapid Screening Method for Examining Corn and Corn-Derived Products for Possible Aflatoxin Contamination

1974 ◽  
Vol 57 (3) ◽  
pp. 764-766
Author(s):  
Roman Barabolak ◽  
Charles R Colburn ◽  
Robert J Smith
Keyword(s):  
Ammonium Sulfate ◽  
Silica Gel ◽  
High Speed ◽  
Screening Method ◽  
Aflatoxin Contamination ◽  
Aqueous Acetone ◽  
Rapid Screening ◽  
Final Solution ◽  
Rapid Screening Method

Abstract Aflatoxins were extracted from the sample by high-speed blending with aqueous acetone, purified with ammonium sulfate, re-extracted into benzene, evaporated to dryness, and redissolved in chloroform-acetone. The final solution was subjected to descending minicolumn chromatography through successive layers of alumina, silica gel, and Florisil, and the aflatoxin band was visualized by fluorescence. The method requires only 35—40 min, and results are semiquantitative.

Sign in / Sign up

Export Citation Format

Share Document