scholarly journals Qualitative And Quantitative Detection of Surgical Pathogenic Microorganisms Escherichia Coli and Staphylococcus Aureus Based on ddPCR System

2020 ◽  
Author(s):  
Tiantian Zhang ◽  
Zhiqiang Niu ◽  
Feng Wu ◽  
Zongkun Chen ◽  
Jun xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Digital Pcr ◽  
Quantitative Detection ◽  
Detection Accuracy ◽  
Pathogenic Microorganisms ◽  
Detection Rates ◽  
Blood Samples ◽  
And Staphylococcus Aureus

Abstract Bacterial culture and drug susceptibility testing are used to identify bacteria associated with infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely used in many fields, including pathogenic microorganism detection, early diagnosis of tumor markers, and analysis of gene expression with its advantages. The purpose of this study was to use a droplet digital PCR system to identify bacteria in blood samples, to explore its ability to identify common pathogenic microorganisms. We designed primers and probes for Escherichia coli and Staphylococcus aureus specific genes for the ddPCR system to identify in blood samples mixed with both organisms. The system had extremely high detection accuracy in samples and the detection rate of E. coli was 13.1–21.4% and that of S. aureus was 50–88.3%. The system identified blood samples containing both bacteria, with detection rates of 18.1%–97%. The ddPCR system qualitatively and quantitatively measured common pathogenic microorganisms in blood samples with high sensitivity and accuracy, providing rapid and accurate detection of pathogenic microorganisms.

scholarly journals Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tiantian Zhang ◽  
Zhiqiang Niu ◽  
Feng Wu ◽  
Zongkun Chen ◽  
Jun Xu ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Bacterial Species ◽  
Screening Method ◽  
Digital Pcr ◽  
Copy Number Variations ◽  
Quantitative Detection ◽  
Detection Accuracy ◽  
Detection Rates ◽  
Blood Samples ◽  
E Coli

AbstractBacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely applied to molecular diagnosis, including copy number variations, single nucleotide variant analysis, cancer biomarker discovery, and pathogen identification. This study aimed to use a droplet digital PCR system to identify bacteria in blood samples and explore its ability to identify pathogen in bacteremia. Then, we designed primers and probes of SWG-9 and COA gene for E. coli and S. aureus to identify in blood samples with the ddPCR system. The system had demonstrated extremely high detection accuracy in blood samples, and the detection rate of E. coli was 13.1–21.4%, and that of S. aureus was 50–88.3%. Finally, blood samples containing both E. coli and S. aureus were tested to evaluate further the accuracy and applicability of this method, indicating the detection rates range from 18.1% to 97%. The ddPCR system is highly promising as a qualitatively and quantitatively screening method for rapidly detecting pathogen.

scholarly journals Pharmacological Role of Anions (Sulphate, Nitrate, Oxalate and Acetate) on the Antibacterial Activity of Cobalt(II), Copper(II) and Nickel(II) Complexes With Nicotinoylhydrazine-Derived ONO, NNO and SNO Ligands

1996 ◽  
Vol 3 (5) ◽  
pp. 211-217 ◽  
Author(s):  
Zahid Hussain Chohan ◽  
Abdur Rauf
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Metal Ion ◽  
Bacterial Species ◽  
Biologically Active ◽  
Schiff Base Ligands ◽  
Mixed Ligands ◽  
And Staphylococcus Aureus

Mixed ligands biologically active complexes of cobalt(II), copper(II) and nickel(II) with nicotinoylhydrazine-derived ONO, NNO and SNO donor schiff-base ligands having the same metal ion but different anions such as sulphate, nitrate, oxalate and acetate have been synthesised and characterised on the basis of their physical, analytical and spectral data. In order to evaluate the role of anions on their bioability, these ligands and their synthesised metal complexes with various anions have been screened against bacterial species such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and the title studies have proved a definative role of anions in increasing the biological activity

scholarly journals EXPERIMENTAL STUDY OF THE PHENOMENON OF MICROORGANISM-ASSOCIATED CRYSTALLOGENESIS

2021 ◽  
Vol 11 (3) ◽  
pp. 20-21
Author(s):  
Andrew Martusevich ◽  
Ivan Bocharin ◽  
Maxim Guryanov ◽  
Alexey Kochkurov ◽  
Elena Kochkurova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Sodium Chloride ◽  
Functional State ◽  
Crystal Formation ◽  
Pathogenic Microorganisms ◽  
Salt Solutions ◽  
E Coli ◽  
And Staphylococcus Aureus

The aim of the study was to clarify the character of the initiatory activity of pathogenic and nonpathogenic microorganisms in regard to salt solutions. We selected colonies of two microorganisms: Escherichia coli (E. coli) and Staphylococcus aureus (St. aureus) [106 , 108 , 1010, 1012, 1014, 1016, 1018 and 1020 CFU/ml of solution]. A 10% solution of sodium chloride was used as the initiated substance, which made it possible to perform a tezigraphic test. On the basis of model experiments with colonies of some pathogenic and non-pathogenic microorganisms, a new phenomenon was confirmed — microbial initiation of crystal formation (the phenomenon of microorganismassociated crystallogenesis). This phenomenon is universal for microagents, but it also has specific features imposed by the species of the microorganism and its current functional state.

scholarly journals Antibacterial effect of Czech and Mānuka honey on selected mastitis pathogens

2018 ◽  
Vol 87 (4) ◽  
pp. 387-393
Author(s):  
Marcela Klimešová ◽  
Irena Němečková ◽  
Eva Vondrušková ◽  
Ludmila Nejeschlebová
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Manuka Honey ◽  
Streptococcus Uberis ◽  
Mastitis Pathogens

The work is focused on the antibacterial effect of four types of blossom honey, one honeydew and two Mānuka honey (MAN100+ and MAN400+) on selected pathogenic microorganisms isolated from cow’s milk (Staphylococcus aureus 51 and S. aureus 428), sheep’s milk (S. aureus 627), and from the Czech Collection of Microorganisms (Streptococcus uberis CCM 4617, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224 and Escherichia coli CCM 4787). The concentrations of honey samples were 20% and 30%. The obtained results showed a 100% inhibitory effect of MAN400+ on all tested bacterial strains even at a concentration of 20% and also a comparable inhibitory effect of Mānuka honey with Czech honeydew. The results indicate that honey had an inhibitory effect against the tested bacterial species which may cause mastitis.

scholarly journals Impact of Subclinical Mastitis-causing Bacterial Species on the Composition and Chemical Properties of Milk

2021 ◽  
Author(s):  
Tarik Safak ◽  
Ali Risvanli ◽  
Zulal Ascı-Toraman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Bacterial Species ◽  
Chemical Properties ◽  
Subclinical Mastitis ◽  
Milk Composition ◽  
Mineral Matter ◽  
California Mastitis Test ◽  
And Staphylococcus Aureus

Background: Here we investigate whether the composition and some chemical properties of milk can correlate with bacterial species causing subclinical mastitis in cows. Methods: One hundred and eighty cows were used in the study. The California Mastitis Test (CMT) was applied to the selected cows. The cows were divided into four groups: cows with negative CMT (n = 45), the Escherichia coli (n = 45), Streptococcus agalactiae (n = 45) and Staphylococcus aureus (n = 45) groups. Result: Milk composition and some chemical properties were measured. The highest fat ratio (5.82±0.34%) was found in the Escherichia coli group (p less than 0.05). The proportions of solid-non-fat (9.67±0.09%), protein (3.51±0.03%), lactose (5.29±05%) and mineral matter (0.75±0.01%) were lowest in the Escherichia coli group (p less than 0.05). The electrical conductivity of milk was lowest in the CMT-negative group (4.23±0.02 mS/cm), while the Streptococcus agalactiae group had the highest value (4.61±0.02 mS/cm) (p less than 0.05). The specific gravity of the Escherichia coli group was lower than the CMT-negative (1,034.91±0.56 kg/m3) and Staphylococcus aureus (1,034.24±0.65 kg/m3) groups (p less than 0.05). Based on our findings, we propose that milk composition and some chemical parameters are altered in subclinical mastitis. However, these could not be standardized according to the responsible bacterial species. We recommend that these parameters are monitored regularly on dairy farms.

Coliform Bacteria and Staphylococcus aureus in Retail Natural Cheeses

1994 ◽  
Vol 57 (3) ◽  
pp. 253-255 ◽  
Author(s):  
DEREK A. BOWEN ◽  
DAVID R. HENNING
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Coliform Bacteria ◽  
Pathogenic Microorganisms ◽  
Canadian Study ◽  
Enrichment Broth ◽  
E Coli ◽  
And Staphylococcus Aureus ◽  
Enrichment Methods ◽  
Potentially Pathogenic Microorganisms

Fifty commercial retail samples of natural cheeses (American and non-American-type) were examined for coliform bacteria, Escherichia coli, E. coli O157:H7, and thermonuclease-positive S. aureus. Nine cheese samples had coliform group bacteria ranging from 10 to 1.1 × 103 CFU/ml by the violet red bile agar procedure. Twenty-four or 48% of the cheeses were positive for coliform group bacteria when a 25-g sample was used in an enrichment broth. One sample had a confirmed E. coli by both the violet red bile agar and enrichment methods. No E. coli O157:H7 were found in the retail natural cheeses examined. Also, no thermonuclease-positive S. aureus were isolated. The levels of potentially pathogenic microorganisms in cheeses were lower in this study than reported in a 1974 to 1976 Canadian study.

scholarly journals Analisis Keefektifan Ekoenzim sebagai Pembersih Kandang Ayam dari Limbah Buah Jeruk (Citrus sp.)

2022 ◽  
Vol 10 (1) ◽  
pp. 42-46
Author(s):  
A. Mahdia ◽  
P. A. Safitri ◽  
R. F. Setiarini ◽  
V. F. A. Maherani ◽  
M. N. Ahsani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Laboratory Testing ◽  
Pathogenic Microorganisms ◽  
Fruit Waste ◽  
Fermentation Period ◽  
Bacteria And Fungi ◽  
Citrus Waste ◽  
And Staphylococcus Aureus ◽  
Number Of Bacteria

Sanitation of the cage usually requires a sanitizer containing a powerful cleaning fluid to sterilize the cage. Materials commonly used for cage sanitation are detergent or disinfectants used to eradicate pathogenic microorganisms that cause bacteria, fungi, or other microorganisms. Eco enzyme is an alternative natural cleaning agent derived from fresh fruit waste through fermentation. This study aimed to make eco enzymes for cleaning chicken coops from citrus waste, characterize the microbiological eco enzymes, and test the effectiveness of eco enzymes as chicken coop cleaners. Eco enzymes from fresh citrus waste after a 3-month fermentation period contained bacteria and fungi of 1.9 x 106CFU/ml and 8.5 x 105CFU/ml, respectively, with a pH of 3.39±0.023. The eco enzyme of cage cleaning fluid from citrus waste (Citrus sp.) can inhibit the growth of Escherichia coli and Staphylococcus aureus through confrontation tests in the laboratory. Testing the effectiveness of eco enzymes in chicken coops can reduce the number of bacteria five times more than detergents for the same area.

Antimicrobial Resistance of Escherichia coli, Enterococci, Pseudomonas aeruginosa, and Staphylococcus aureus from Raw Fish and Seafood Imported into Switzerland

2016 ◽  
Vol 79 (7) ◽  
pp. 1240-1246 ◽  
Author(s):  
RENATE BOSS ◽  
GUDRUN OVERESCH ◽  
ANDREAS BAUMGARTNER
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Health Concern ◽  
E Coli ◽  
And Staphylococcus Aureus

ABSTRACT A total of 44 samples of salmon, pangasius (shark catfish), shrimps, and oysters were tested for the presence of Escherichia coli, enterococci, Pseudomonas aeruginosa, and Staphylococcus aureus, which are indicator organisms commonly used in programs to monitor antibiotic resistance. The isolated bacterial strains, confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, were tested against a panel of 29 antimicrobial agents to obtain MICs. Across the four sample types, Enterococcus faecalis (59%) was most common, followed by E. coli (55%), P. aeruginosa (27%), and S. aureus (9%). All bacterial species were resistant to some antibiotics. The highest rates of resistance were in E. faecalis to tetracycline (16%), in E. coli to ciprofloxacin (22%), and in S. aureus to penicillin (56%). Antibiotic resistance was found among all sample types, but salmon and oysters were less burdened than were shrimps and pangasius. Multidrug-resistant (MDR) strains were exclusively found in shrimps and pangasius: 17% of pangasius samples (MDR E. coli and S. aureus) and 64% of shrimps (MDR E. coli, E. faecalis, and S. aureus). Two of these MDR E. coli isolates from shrimps (one from an organic sample) were resistant to seven antimicrobial agents. Based on these findings, E. coli in pangasius, shrimps, and oysters, E. faecalis in pangasius, shrimps, and salmon, and P. aeruginosa in pangasius and shrimps are potential candidates for programs monitoring antimicrobial resistance. Enrichment methods for the detection of MDR bacteria of special public health concern, such as methicillin-resistant S. aureus and E. coli producing extended-spectrum β-lactamases and carbapenemases, should be implemented.

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