scholarly journals Analisis Keefektifan Ekoenzim sebagai Pembersih Kandang Ayam dari Limbah Buah Jeruk (Citrus sp.)

2022 ◽  
Vol 10 (1) ◽  
pp. 42-46
Author(s):  
A. Mahdia ◽  
P. A. Safitri ◽  
R. F. Setiarini ◽  
V. F. A. Maherani ◽  
M. N. Ahsani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Laboratory Testing ◽  
Pathogenic Microorganisms ◽  
Fruit Waste ◽  
Fermentation Period ◽  
Bacteria And Fungi ◽  
Citrus Waste ◽  
And Staphylococcus Aureus ◽  
Number Of Bacteria

Sanitation of the cage usually requires a sanitizer containing a powerful cleaning fluid to sterilize the cage. Materials commonly used for cage sanitation are detergent or disinfectants used to eradicate pathogenic microorganisms that cause bacteria, fungi, or other microorganisms. Eco enzyme is an alternative natural cleaning agent derived from fresh fruit waste through fermentation. This study aimed to make eco enzymes for cleaning chicken coops from citrus waste, characterize the microbiological eco enzymes, and test the effectiveness of eco enzymes as chicken coop cleaners. Eco enzymes from fresh citrus waste after a 3-month fermentation period contained bacteria and fungi of 1.9 x 106CFU/ml and 8.5 x 105CFU/ml, respectively, with a pH of 3.39±0.023. The eco enzyme of cage cleaning fluid from citrus waste (Citrus sp.) can inhibit the growth of Escherichia coli and Staphylococcus aureus through confrontation tests in the laboratory. Testing the effectiveness of eco enzymes in chicken coops can reduce the number of bacteria five times more than detergents for the same area.

scholarly journals EXPERIMENTAL STUDY OF THE PHENOMENON OF MICROORGANISM-ASSOCIATED CRYSTALLOGENESIS

2021 ◽  
Vol 11 (3) ◽  
pp. 20-21
Author(s):  
Andrew Martusevich ◽  
Ivan Bocharin ◽  
Maxim Guryanov ◽  
Alexey Kochkurov ◽  
Elena Kochkurova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Sodium Chloride ◽  
Functional State ◽  
Crystal Formation ◽  
Pathogenic Microorganisms ◽  
Salt Solutions ◽  
E Coli ◽  
And Staphylococcus Aureus

The aim of the study was to clarify the character of the initiatory activity of pathogenic and nonpathogenic microorganisms in regard to salt solutions. We selected colonies of two microorganisms: Escherichia coli (E. coli) and Staphylococcus aureus (St. aureus) [106 , 108 , 1010, 1012, 1014, 1016, 1018 and 1020 CFU/ml of solution]. A 10% solution of sodium chloride was used as the initiated substance, which made it possible to perform a tezigraphic test. On the basis of model experiments with colonies of some pathogenic and non-pathogenic microorganisms, a new phenomenon was confirmed — microbial initiation of crystal formation (the phenomenon of microorganismassociated crystallogenesis). This phenomenon is universal for microagents, but it also has specific features imposed by the species of the microorganism and its current functional state.

INTERRELATION BETWEEN BACTERIAL ATP AND LEVEL OF MICROORGANISM CELLS IN THE WASHINGS OF PURE AND MIXED CULTURES OF ESCHERICHIA AND STAPHYLOCOCCUS AUREUS

2018 ◽  
Vol 1 (2) ◽  
pp. 74-78
Author(s):  
V. M. Sotnikova ◽  
◽  
N. A. Shurduba ◽  
D. V. Gruznov ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Linear Dependence ◽  
Mixed Cultures ◽  
Special Test ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Atp Bioluminescence ◽  
And Staphylococcus Aureus ◽  
Number Of Bacteria

Тhe article presents the results of studies to determine the amount of bacterial ATP in pure and mixed cultures of microorganisms. The level of ATP-bioluminescence was determined using the HY-LiTE®2 (Merk, Germany) luminometer in accordance with the manufacturer's instructions using special test systems. In the course of the experiments, the numerical values of bacterial ATP of Escherichia coli and Staphylococcus aureus have been determined. It was found that at the concentration of E. coli (1,5 ± 0,1)∙102…(2,1 ± 0,2)∙106 CFU / cm3, the level of ATP was 323,9 ± 16,2…22666,8 ± 906,7 RLU. At equal number of bacteria, the level of ATP St. aureus exceeded the level of ATP escherichia by 1,4…1,8 times. It was noted that in mixed cultures of Escherichia coli and staphylococcus, the values of ATP were average values between ATP escherichia and staphylococcal ATP and were within 423,4 ± 29,6…28334,1 ± 2550,1 RLU with the number of microorganisms (1,75 ± 0, 1) ∙ 102…(1,5 ± 0,1)∙106 CFU/cm3. It was found that in pure and mixed cultures of microorganisms between the level of bacteria and the parameters of ATP there is a linear dependence: the more the number of bacteria, the higher the level of ATP.

scholarly journals Qualitative And Quantitative Detection of Surgical Pathogenic Microorganisms Escherichia Coli and Staphylococcus Aureus Based on ddPCR System

2020 ◽  
Author(s):  
Tiantian Zhang ◽  
Zhiqiang Niu ◽  
Feng Wu ◽  
Zongkun Chen ◽  
Jun xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Digital Pcr ◽  
Quantitative Detection ◽  
Detection Accuracy ◽  
Pathogenic Microorganisms ◽  
Detection Rates ◽  
Blood Samples ◽  
And Staphylococcus Aureus

Abstract Bacterial culture and drug susceptibility testing are used to identify bacteria associated with infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely used in many fields, including pathogenic microorganism detection, early diagnosis of tumor markers, and analysis of gene expression with its advantages. The purpose of this study was to use a droplet digital PCR system to identify bacteria in blood samples, to explore its ability to identify common pathogenic microorganisms. We designed primers and probes for Escherichia coli and Staphylococcus aureus specific genes for the ddPCR system to identify in blood samples mixed with both organisms. The system had extremely high detection accuracy in samples and the detection rate of E. coli was 13.1–21.4% and that of S. aureus was 50–88.3%. The system identified blood samples containing both bacteria, with detection rates of 18.1%–97%. The ddPCR system qualitatively and quantitatively measured common pathogenic microorganisms in blood samples with high sensitivity and accuracy, providing rapid and accurate detection of pathogenic microorganisms.

Coliform Bacteria and Staphylococcus aureus in Retail Natural Cheeses

1994 ◽  
Vol 57 (3) ◽  
pp. 253-255 ◽  
Author(s):  
DEREK A. BOWEN ◽  
DAVID R. HENNING
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Coliform Bacteria ◽  
Pathogenic Microorganisms ◽  
Canadian Study ◽  
Enrichment Broth ◽  
E Coli ◽  
And Staphylococcus Aureus ◽  
Enrichment Methods ◽  
Potentially Pathogenic Microorganisms

Fifty commercial retail samples of natural cheeses (American and non-American-type) were examined for coliform bacteria, Escherichia coli, E. coli O157:H7, and thermonuclease-positive S. aureus. Nine cheese samples had coliform group bacteria ranging from 10 to 1.1 × 103 CFU/ml by the violet red bile agar procedure. Twenty-four or 48% of the cheeses were positive for coliform group bacteria when a 25-g sample was used in an enrichment broth. One sample had a confirmed E. coli by both the violet red bile agar and enrichment methods. No E. coli O157:H7 were found in the retail natural cheeses examined. Also, no thermonuclease-positive S. aureus were isolated. The levels of potentially pathogenic microorganisms in cheeses were lower in this study than reported in a 1974 to 1976 Canadian study.

Comparison of the Effects of Silver in Nanostructured and Ultrahigh Diluted Form on Growth and Volatile Compounds Produced by Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 316-329
Author(s):  
Fateme Mirzajani ◽  
Amin Hamidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Life Cycles ◽  
Gas Chromatography Mass Spectrometry ◽  
Untreated Sample ◽  
E Coli ◽  
Volatile Metabolites ◽  
Octyl Acetate ◽  
Volatile Organic ◽  
And Staphylococcus Aureus

Introduction: In this project, the growth and volatile metabolites profiles of Escherichia coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical, nanoparticle and ultra-highly diluted compounds. Materials & Methods: The treatments were done for 12000 life cycles using silver nanoparticles (AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later. Also, with the continuous usage of these materials, no more bacterial growth was observed. Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate, Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol. The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde, 2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were common and produced by both organisms. Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).

Penggunaan Limbah Logam Tembaga yang Didaur Ulang untuk Antibakteri dan Degradasi Metil Merah Secara Fotolisis

2019 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Ariyetti Ariyetti ◽  
Muhammad Nasir ◽  
Safni Safni ◽  
Syukri Darajat
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nitrate ◽  
Copper Sulfate ◽  
Bacterial Growth ◽  
Methyl Red ◽  
Copper Metal ◽  
Sulfate Hydrate ◽  
Red Dye ◽  
And Staphylococcus Aureus

<p><em>Metil merah merupakan salah satu zat warna golongan azo yang sering digunakan dalam industri dan laboratorium. Penggunaan metil merah dapat menimbulkan efek terhadap kesehatan dan lingkungan. Oleh sebab itu dilakukan metode fotodegradasi dengan menggunakan semikonduktor dan radiasi sinar tampak. Semikonduktor yang digunakan yaitu berbahan dasar tembaga sulfat hidrat dan perak nitrat. Prekusor tembaga sulfat hidrat dibuat dari pengolahan limbah logam tembaga hasil pemotongan tembaga yang ada di bengkel Lembaga Ilmu Pengetahuan Indonesia (LIPI) Bandung. Bahan semikonduktor juga memiliki kemampuan dalam menghambat pertumbuhan bakteri. Hasil optimum yang didapatkan dalam proses fotodegradasi dan antibakteri merupakan gabungan antara kedua prekusor tembaga sulfat hidrat dan perak nitrat dengan bantuan penyinaran. Kemampuan dalam menghambat pertumbuhan bakteri didapatkan persentase kematian 100 % untuk masing-masing bakteri, yaitu Escherichia coli dan Staphylococcus aureus. Aktifitas fotokatalitiknya dengan konsentrasi semikonduktor 10 ppm untuk mendegradasi zat warna metil merah 5 ppm, selama 23 jam, dimana persentase degradasi yang didapatkan dengan penyinaran lebih tinggi dibandingkan dengan tanpa penyinaran. Pengaruh pH larutan terhadap degradasi metil merah yaitu optimum pada pH 12 (basa).</em></p><p><em><br /></em></p><p><em>Methyl red is one of the azo group dyes that is often used in industry and laboratories. The use of methyl red can have an effect on health and the environment. Therefore photodegradation method is done by using semiconductor and visible light radiation. The semiconductor used is based on copper sulfate hydrate and silver nitrate. The copper sulphate hydrate precursor is made from the processing of copper-cut copper metal waste in the workshop of the Indonesian Institute of Sciences (LIPI) in Bandung. Semiconductor materials also have the ability to inhibit bacterial growth. The optimum results obtained in the photodegradation and antibacterial process are a combination of both copper sulfate hydrate precursor and silver nitrate with the help of irradiation. The ability to inhibit bacterial growth obtained 100% mortality for each bacterium, namely Escherichia coli and Staphylococcus aureus. Photocatalytic activity with 10 ppm semiconductor concentration to degrade methyl red dye 5 ppm, for 23 hours, where the percentage of degradation obtained by irradiation is higher than without irradiation. The effect of pH of the solution on the degradation of methyl red is optimum at pH 12 (base).</em></p>

scholarly journals Sulfaquinoxaline Oxidation and Toxicity Reduction by Photo-Fenton Process

2021 ◽  
Vol 18 (3) ◽  
pp. 1005
Author(s):  
Vanessa Ribeiro Urbano ◽  
Milena Guedes Maniero ◽  
José Roberto Guimarães ◽  
Luis J. del Valle ◽  
Montserrat Pérez-Moya
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pilot Plant ◽  
Environmental Water ◽  
Fenton Process ◽  
Experimental Conditions ◽  
Laboratory Bench ◽  
Degradation Rates ◽  
Bacterial Growth Inhibition ◽  
And Staphylococcus Aureus

Sulfaquinoxaline (SQX) has been detected in environmental water samples, where its side effects are still unknown. To the best of our knowledge, its oxidation by Fenton and photo-Fenton processes has not been previously reported. In this study, SQX oxidation, mineralization, and toxicity (Escherichia coli and Staphylococcus aureus bacteria) were evaluated at two different setups: laboratory bench (2 L) and pilot plant (15 L). The experimental design was used to assess the influence of the presence or absence of radiation source, as well as different H2O2 concentrations (94.1 to 261.9 mg L−1). The experimental conditions of both setups were: SQX = 25 mg L−1, Fe(II) = 10 mg L−1, pH 2.8 ± 0.1. Fenton and photo-Fenton were suitable for SQX oxidation and experiments resulted in higher SQX mineralization than reported in the literature. For both setups, the best process was the photo-Fenton (178.0 mg L−1 H2O2), for which over 90% of SQX was removed, over 50% mineralization, and bacterial growth inhibition less than 13%. In both set-ups, the presence or absence of radiation was equally important for sulfaquinoxaline oxidation; however, the degradation rates at the pilot plant were between two to four times higher than the obtained at the laboratory bench.

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