scholarly journals Microbiome Analysis of Three Sample Types Obtained From Mixed Dentition Children With Different Caries Statuses: A Cross-Sectional Survey

2020 ◽  
Author(s):  
Xiaoxia Yang ◽  
Lidan He ◽  
Siqi Yan ◽  
Xinyi Chen ◽  
Guoying Que
Keyword(s):  
Streptococcus Mutans ◽  
Mixed Dentition ◽  
Deciduous Teeth ◽  
Scaling Analysis ◽  
Healthy Children ◽  
Cross Sectional Survey ◽  
Microbial Composition ◽  
Cross Sectional ◽  
Supragingival Plaque ◽  
Selenomonas Noxia

Abstract Background: Both supragingival plaque and saliva are commonly sampled for analysis of microbial communities. However, it remains unclear whether saliva can replace supragingival plaque for microbial studies of caries. There are limited studies on whether the microbiota of healthy first permanent molar (FPM) differs in different caries statuses of deciduous teeth. Herein, the PacBio Sequel platform was used to identify microbiome between three types of oral samples. Additionally, we compared the microbia between children with caries and healthy children in the same kind of microhabitat.Methods: In total, 30 children (aged 7–9 years) were enrolled in this study; 15 of them had dental caries. DNA was extracted from supragingival plaques of deciduous molars, supragingival plaques of maxillary FPMs, and saliva, and the v1–v9 regions of 16S rRNA was amplified. Additionally, PacBio sequencing and bioinformatic analyses were performed.Results: The salivary microbial alpha diversity were lower than that of the supragingival plaque on the teeth, but three was no difference between deciduous teeth and FPMs. Nonmetric multidimensional scaling analysis showed that the saliva samples were mainly scattered in the left side, whereas most plaque samples were distributed on the right. In the same microhabitat , there was no difference in microbial alpha and beta diverty between children with caries and healthy children. For the samples of the deciduous teeth, Streptococcus mutans, Propionibacterium acidifaciens, and Veillonella dispar were more abundant in the children with caries than healthy children, and the first two bacteria showed a positive correlation. For the samples of the FPMs, Selenomonas noxia was more abundant in healthy children than children with caries. But no differentially abundant microorganism were identified between the saliva subgroups. Conclusion:The microbial composition and struction of supragingival plaque was different from that of saliva, and supragingival plaque was found to be the best candidate for studying caries etiology. Streptococcus mutans, Veillonella dispar, and Propionibacterium acidifaciens are highly associated with the existence of deciduous caries. The microbia of the supragingival plaque on the healthy FPMs resemble when the caries status of the deciduous teeth was different, except for the abundance of Selenomonas noxia.

scholarly journals The impact of caries status on supragingival plaque and salivary microbiome in children with mixed dentition: a cross-sectional survey

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaoxia Yang ◽  
Lidan He ◽  
Siqi Yan ◽  
Xinyi Chen ◽  
Guoying Que
Keyword(s):  
Oral Microbiome ◽  
Mixed Dentition ◽  
Deciduous Teeth ◽  
Healthy Children ◽  
Pacbio Sequencing ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Alpha And Beta Diversity ◽  
Supragingival Plaque ◽  
The Impact

Abstract Background Supragingival plaque and saliva are commonly used for microbiome analysis. Many epidemiological studies have identified deciduous teeth caries as a risk factor for caries development in first permanent molar (FPM); nevertheless, to the best of our knowledge, there are no reports on the effects of deciduous teeth caries on the microbiome of healthy FPM. Additionally, it remains unclear whether saliva can be used instead of supragingival plaque for caries microbial studies. Therefore, we aimed to elucidate this issue, and to characterize and compare the oral microbiome of healthy FPMs in children with different caries statuses and that from children with and without caries in a similar microhabitat, by PacBio sequencing. Currently, few studies have investigated the oral microbiome of children using this technique. Methods Thirty children (aged 7–9 years) with mixed dentition were enrolled; 15 had dental caries, and 15 did not. Supragingival plaques of deciduous molars and maxillary FPMs, and non-stimulating saliva samples were collected. DNA was extracted and the v1–v9 regions of 16S rRNA were amplified. Subsequently, PacBio sequencing and bioinformatic analyses were performed for microbiome identification. Results The microbial alpha diversity of the saliva samples was lower than that of the supragingival plaque (p < 0.05); however, no differences were detected between deciduous teeth and FPMs (p > 0.05). In addition, the alpha and beta diversity of children with and without caries was also similar (p > 0.05). Nonmetric multidimensional scaling and Adonis analyses indicated that the microbial structure of salivary and supragingival plaque samples differ (p < 0.05). Further analysis of deciduous teeth plaque showed that Streptococcus mutans, Propionibacterium acidifaciens, and Veillonella dispar were more abundant in children with caries than in those without (p < 0.05); while in FPMs plaque, Selenomonas noxia was more abundant in healthy children (p < 0.05). No differences in microorganisms abundance were found in the saliva subgroups (p > 0.05). Conclusion We have determined that supragingival plaque was the best candidate for studying carious microbiome. Furthermore, S. mutans, V. dispar, and P. acidifaciens were highly associated with deciduous teeth caries. S. noxia may be associated with the abiding health of FPM; however, this requires additional studies.

scholarly journals The Status and Associated Factors  of Early Childhood Caries among 3- to 5-year-old Children in Guangdong, Southern  China: A Provincial Cross-sectional Survey

2020 ◽  
Author(s):  
Jianbo Li ◽  
Weihua Fan ◽  
Yueshan Zhou ◽  
Linmei Wu ◽  
Wei Liu ◽  
...  
Keyword(s):  
Preschool Children ◽  
Oral Health ◽  
Associated Factors ◽  
Early Childhood Caries ◽  
Southern China ◽  
Guangdong Province ◽  
Deciduous Teeth ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
The Mean

Abstract Background: Dental caries of deciduous teeth (Early Childhood Caries, ECC) has become a crucial oral health problem over the decades in China. The aim of this study was to examine the prevalence and severity of ECC among preschool children from Guangdong Province, Southern China. In addition, to assess the association of ECC with reported oral health-related behaviors.Methods: A cross-sectional survey was carried out in Guangdong Province by means of an equal-sized, stratified, multistage random sampling method. According to the standard for clinical dentition examination of the WHO 2013 criteria, the presence of ECC was determined by the dmft (decayed-missing-filled tooth) index using a CPI (Community Periodontal Index) probe. A questionnaire about caries-related factors was completed by each of the participants’ parents or grandparents through a face-to-face and one-on-one interview. Then, t-test, Chi2 tests, One-Way ANOVA served for statistical analysis, and logistic regression analysis as well as covariance analysis were executed to identify potential associated factors for ECC.Results: There were 2592 3- to 5-year-old preschool children. The prevalence (% dmft >0) of ECC was 68.3 (95% CI: 66.5-70.1), the mean dmft was 4.36 (95% CI: 4.17-4.55), and the filled rate was 1.2%. In multivariable modeling, associated factors for both prevalence and mean dmft were older age, rural areas, consumption of sweets before sleep, dental visit history, low household income, and low parental education level. Initiating toothbrushing after 3 years of age and being exclusively/ predominantly breastfed indicated only the prevalence; being female and frequently consuming sweetened milk/powdered milk indicated only the mean dmft.Conclusions: The preschool children in Guangdong Province experienced a significant amount of ECC. More attention should be given to children’s dental health for deciduous teeth. Associated factors for ECC included demographics, oral health measures, dietary factors, and socioeconomic factors.

scholarly journals THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

2020 ◽  
pp. 20-23
Author(s):  
Sneha Upadhyay ◽  
Jyoti Bhavthankar ◽  
Mandakini Mandale ◽  
Nivedita Kaorey
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cross Sectional Study ◽  
Control Group ◽  
Healthy Children ◽  
Sectional Study ◽  
Cross Sectional ◽  
Asthmatic Children ◽  
Significant Difference ◽  
The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

scholarly journals Prevalence of malocclusion and occlusal traits in the early mixed dentition in Shanghai, China

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6630
Author(s):  
Xin Yu ◽  
Hao Zhang ◽  
Liangyan Sun ◽  
Jie Pan ◽  
Yuehua Liu ◽  
...  
Keyword(s):  
Primary Schools ◽  
Sagittal Plane ◽  
Epidemiological Data ◽  
Open Bite ◽  
Mixed Dentition ◽  
Class Iii ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Anterior Crowding ◽  
Posterior Crossbite

BackgroundEpidemiological data on malocclusion among Chinese children are scant. The aim of this study was to provide detailed information on the prevalence of malocclusion in early mixed dentition children in Shanghai, China.MethodsA cross-sectional survey was conducted from September 2016 to April 2017, and 2,810 children aged 7- to 9- years were selected from 10 primary schools by cluster random sampling. Several occlusal parameters, including Angle molar relationship, overjet, overbite, open bite, anterior and posterior crossbite, midline displacement, scissors bite, and teeth crowding and spacing, were clinically registered by five calibrated orthodontic dentists.ResultsWe found that 79.4% children presented one or more occlusal anomalies. Angle Class I, Class II and Class III molar relationship were recorded in 42.3%, 50.9% and 5.9% of the sample, respectively. The proportion of Class III increased from 5.0% at age 7 to 7.8% at age 9. In the sagittal plane, increased overjet >3 mm was observed in 40.8% subjects, while the prevalence of severe overjet (>8 mm), anterior edge-to-edge (zero overjet) and anterior crossbite were 5.2%, 8.1% and 10.5%, respectively. Vertically, deep overbite >2/3 overlap was found in 6.2% of the children and open bite in 4.3%. Boys exhibited a higher rate of overbite than girls. For the transversal occlusal anomalies, 36.1% of the children had a midline displacement, which was followed by posterior crossbite (2.6%) and scissors bite (1.0%). Teeth space discrepancies were also common anomalies and anterior crowding (>2 mm) affecting 28.4% of the children, while anterior spacing (>4 mm) affecting 9.5%. Girls showed a higher prevalence of anterior crowding and a lower frequency of teeth spacing than boys.ConclusionsOur study demonstrated that malocclusion is prevalent among children in the early mixed dentition, and more health resources should be warranted to meet the challenge of prevention or early intervention of malocclusion.

scholarly journals The prevalence of orthodontic treatment need and malocclusion problems in 8–9-year-old schoolchildren: A study in the south of Thailand

2019 ◽  
Vol 9 ◽  
pp. 99-104 ◽  
Author(s):  
Sirate Rapeepattana ◽  
Angkana Thearmontree ◽  
Supanee Suntornlohanakul
Keyword(s):  
Orthodontic Treatment ◽  
Class Ii ◽  
Mixed Dentition ◽  
Class I ◽  
Class Iii ◽  
The South ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Treatment Need ◽  
Normal Occlusion

Aims This study aims to find the prevalence of orthodontic treatment need and malocclusion problems in 8–9-year-old schoolchildren in the south of Thailand. Materials and Methods A number of 202 children (100 boys and 102 girls) samples were randomly selected from all schools in Hat Yai District, Songkhla Province, Thailand. A cross-sectional survey of dental health component (DHC) of the Index of Orthodontic Treatment Need (IOTN) and malocclusion problems was investigated by clinical examination and dental model. Results Levels 4 and 5 of orthodontic treatment need according to DHC of IOTN of the sample were presented in 18.8% and 1.49%, respectively. Children who need orthodontic treatment (Grade 2–4) showed more than one highest DHC problem that indicated the level of treatment need (39.68%). Normal occlusion was found at 6.43%. Malocclusions such as Class I, Class II division 1, Class II division 2, and Class III malocclusion were observed in 78.71%, 7.92%, 3.47%, and 3.47%, respectively. Reversed overjet and overjet >9 mm were detected in 5.64% and 1.58%, respectively. Approximately half of the children (46.67%) had overbite >3.5 mm. Conclusions High percentage of children in mixed dentition period who need orthodontic treatment was found in this study. Some children who presented with the orthodontic treatment need Grade 2–4 had more than one DHC problem which identified the grade of treatment need. Class I malocclusion was most frequently found in this group of children.

Caries-associated Salivary Microbiota of Children at Mixed Dentition from Different Geographic Locations

2021 ◽  
Author(s):  
Shanshan Li ◽  
Shi Huang ◽  
Ying Zhang ◽  
Lijuan Zhang ◽  
Fan Li ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Alpha Diversity ◽  
Mixed Dentition ◽  
Diagnostic Model ◽  
Human Populations ◽  
Healthy Children ◽  
Microbial Composition ◽  
Model Based ◽  
Geographical Regions ◽  
Salivary Microbiota

Abstract The microbial composition of dental caries may depend on age, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we profiled and compared saliva microbiota from 130 individuals aged 6 to 8 years old, representing both healthy children (H) and children with severe caries (C) from two geographical regions of China: Qingdao Guangzhou. First, the saliva microbiota exhibited profound differences in diversity and composition between the C and H groups. The caries microbiota featured a lower alpha diversity and more variable community structure than the healthy microbiota. Furthermore, the relative abundance of several genera (e.g., Lactobacillus, Gemella and Cryptobacterium) was significantly higher in the C group than in the H group. Next, geography dominated over disease status in shaping salivary microbiota, and a wide array of salivary bacteria was highly predictive of the individuals’ city of origin. Finally, we built a universal diagnostic model based on 14 bacterial species, which can diagnose caries with 87% and 85% accuracy within each city and 83% accuracy across cities. These findings demonstrated that despite the large effect size of geography, a universal model based on salivary microbiota has the potential to diagnose caries across human populations.

scholarly journals Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition

2021 ◽  
Vol 11 ◽  
Author(s):  
Shanshan Li ◽  
Shi Huang ◽  
Yi Guo ◽  
Ying Zhang ◽  
Lijuan Zhang ◽  
...  
Keyword(s):  
Dental Caries ◽  
Bacterial Species ◽  
Mixed Dentition ◽  
Diagnostic Model ◽  
Healthy Children ◽  
Oral Diseases ◽  
Microbial Composition ◽  
Model Based ◽  
Geographical Regions ◽  
Salivary Microbiota

Dental caries is one of the most prevalent chronic oral diseases, affecting approximately half of children worldwide. The microbial composition of dental caries may depend on age, oral health, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we profiled and compared saliva microbiota from 130 individuals aged 6 to 8 years old, representing both healthy children (H group) and children with caries-affected (C group) from two geographical regions of China: a northern city (Qingdao group) and a southern city (Guangzhou group). First, the saliva microbiota exhibited profound differences in diversity and composition between the C and H groups. The caries microbiota featured a lower alpha diversity and more variable community structure than the healthy microbiota. Furthermore, the relative abundance of several genera (e.g., Lactobacillus, Gemella, Cryptobacterium and Mitsuokella) was significantly higher in the C group than in the H group (p&lt;0.05). Next, geography dominated over disease status in shaping salivary microbiota, and a wide array of salivary bacteria was highly predictive of the individuals’ city of origin. Finally, we built a universal diagnostic model based on 14 bacterial species, which can diagnose caries with 87% (AUC=86.00%) and 85% (AUC=91.02%) accuracy within each city and 83% accuracy across cities (AUC=92.17%). Although the detection rate of Streptococcus mutans in populations is not very high, it could be regarded as a single biomarker to diagnose caries with decent accuracy. These findings demonstrated that despite the large effect size of geography, a universal model based on salivary microbiota has the potential to diagnose caries across the Chinese child population.

scholarly journals Impact of Picky Eating on Level of Family Stress in Healthy Children between the Ages of 3 and 6 Years

2014 ◽  
Vol 8 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Rocío Ramos-Paúl ◽  
Barbara J. Marriage ◽  
Roberto Ruiz Debeza ◽  
Liliana Oliveros Leal ◽  
Luis Ros Mar ◽  
...  
Keyword(s):  
Family Stress ◽  
Eating Habits ◽  
Survey Study ◽  
Healthy Children ◽  
Food Record ◽  
Cross Sectional Survey ◽  
Family Dynamic ◽  
Cross Sectional ◽  
Picky Eating ◽  
The Impact

Objective. One of the goals of this study was to measure the impact of a child’s eating habits on a family’s level of stress related to meal times in the home. Methods. A cross-sectional survey study was conducted on 1,090 children between the ages of three and six years, in Madrid, Spain from 2010 to 2011. Questionnaires about the child’s eating habits, family stress, and medical history, along with a three-day food record were filled out by parents. Results. Levels of family stress related to meal time occasions were statistically higher in the picky eater (PE) group compared to the healthy eater (HE) group (p=0.007). Conclusion. This study demonstrates a possible relationship between picky eating behaviors and family stress in the home. Higher levels of family stress can have long-term negative behavioral consequences or changes on the family dynamic, which may further exacerbate feeding disorders.

scholarly journals Oral health status of disabled children

1970 ◽  
Vol 36 (2) ◽  
pp. 61-63 ◽  
Author(s):  
Sultana Gul Nahar ◽  
Md Amzad Hossain ◽  
Mohammod Borhan Uddin Howlader ◽  
Anam Ahmed
Keyword(s):  
Cross Sectional Study ◽  
Oral Health Status ◽  
Mixed Dentition ◽  
Healthy Children ◽  
Disabled Children ◽  
Normal Children ◽  
Cross Sectional ◽  
Decayed Tooth ◽  
The Disabled ◽  
Index Value

110 disabled and 110 normal healthy children with the age range from 3 to 14 were examined in this cross sectional study. The average number of decayed tooth found 5.6 in deciduous and 6.5 in mixed dentition in disabled children, and the numbers were 3.5 and 4.0 respectively in normal children. Average number of missing tooth was 1.3 in disabled and was 1.0 normal child. The oral hygiene index value was 3 in disabled and 2.47 were in healthy children. In the disabled children, 36% respondents had normal gingiva, 50% had mild gingivitis and 14% had marked gingivitis; but in the normal children, about 73% showed normal gingiva, 23% developed mild gingivitis and only 4.5% had marked gingivitis. DOI: 10.3329/bmrcb.v36i2.6989Bangladesh Med Res Counc Bull 2010; 36: 61-63

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