scholarly journals Integrative Transcriptome Analyses of Metabolic Responses in Yak Liver Define Putative Pivotal LncRNAs

2020 ◽  
Author(s):  
Li Wang ◽  
Wei Xia ◽  
Fang Fu ◽  
Xiaolin Luo ◽  
Jiuqiang Guan
Keyword(s):  
Metabolic Responses ◽  
Qrt Pcr ◽  
The People ◽  
Genome Wide ◽  
Plateau Area ◽  
Physiological Homeostasis ◽  
Transcriptome Analyses ◽  
Metabolic Regulators ◽  
Integrative Transcriptome Analyses

Abstract Background The yak (Bos grunniens) is a crucial resource to supply meat and milk to the people localized in Tibetan plateau area. To systemically identify lncRNAs regulating metabolism in yak, we performed transcriptome analyses to simultaneously profile mRNAs and lncRNAs of liver in yak under three representative age (LD: Liver 1 Day, LM: Liver 15 Month, LY: Liver 5 Year) conditions. Result Of 288 differentially regulated lncRNAs, function-oriented filters yield 88 regulated metabolically related lncRNAs that are differentially expressed at least two age conditions. These lncRNAs predicted by lncRNA-mRNA correlation analyses to function in diverse aspects of metabolism. Specific regulations of liver metabolically related lncRNAs were further defined by qRT-PCR. Conclusion Combining genome-wide screens, bioinformatics function predictions, and qRT-PCR analyses, this study supports that a class of lncRNAs function as important metabolic regulators and establishes a framework for systemically investigating the role of lncRNAs in physiological homeostasis of yak.

scholarly journals LncRNAs Function as Critical Metabolic Regulators in yak Liver by Integrative Transcriptome Analysis

2020 ◽  
Author(s):  
Wei Xia ◽  
Fang Fu ◽  
Li Wang ◽  
Xiaolin Luo ◽  
Jiuqiang Guan
Keyword(s):  
Tibetan Plateau ◽  
Correlation Analysis ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Qrt Pcr ◽  
The People ◽  
Plateau Area ◽  
Metabolic Regulators

Abstract Background: The yak (Bos grunniens) is a crucial resource to supply meat and milk to the people localized in Qinghai-Tibetan plateau area. To identify lncRNAs regulating metabolism in yak, this work adopted transcriptome method to simultaneously profile mRNAs and lncRNAs of liver in yak under three representative age (LD: Liver 1 Day, LM: Liver 15 Months, LY: Liver 5 Years) conditions.Result: Of 288 differentially expressed lncRNAs, function-oriented selection yield 88 regulated metabolically related lncRNAs that were differentially expressed at least two age conditions. These lncRNAs predicted by lncRNA-mRNA correlation analysis to function in various aspects of metabolism. Selected regulations of liver metabolically related lncRNAs were further verified by qRT-PCR.Conclusion: Combining high throughput RNA-seq screening screens, bioinformatics predictions, lncRNA-mRNA correlation analysis and qRT-PCR analysis, this study supports that a class of lncRNAs function as important metabolic regulators and establishes a foundation for further investigating the role of lncRNAs in yak.

scholarly journals Integrative Transcriptome Analyses of Metabolic Responses in Mice Define Pivotal LncRNA Metabolic Regulators

2016 ◽  
Vol 24 (4) ◽  
pp. 627-639 ◽  
Author(s):  
Ling Yang ◽  
Ping Li ◽  
Wenjing Yang ◽  
Xiangbo Ruan ◽  
Kurtis Kiesewetter ◽  
...  
Keyword(s):  
Metabolic Responses ◽  
Transcriptome Analyses ◽  
Metabolic Regulators ◽  
Integrative Transcriptome Analyses

scholarly journals Listeria monocytogenes σB Modulates PrfA-Mediated Virulence Factor Expression

2009 ◽  
Vol 77 (5) ◽  
pp. 2113-2124 ◽  
Author(s):  
Juliane Ollinger ◽  
Barbara Bowen ◽  
Martin Wiedmann ◽  
Kathryn J. Boor ◽  
Teresa M. Bergholz
Keyword(s):  
Gene Expression ◽  
Listeria Monocytogenes ◽  
Transcriptional Activation ◽  
Virulence Gene ◽  
Transcript Profiling ◽  
Virulence Gene Expression ◽  
Qrt Pcr ◽  
Genome Wide

ABSTRACT Listeria monocytogenes σB and positive regulatory factor A (PrfA) are pleiotropic transcriptional regulators that coregulate a subset of virulence genes. A positive regulatory role for σB in prfA transcription has been well established; therefore, observations of increased virulence gene expression and hemolytic activity in a ΔsigB strain initially appeared paradoxical. To test the hypothesis that L. monocytogenes σB contributes to a regulatory network critical for appropriate repression as well as induction of virulence gene expression, genome-wide transcript profiling and follow-up quantitative reverse transcriptase PCR (qRT-PCR), reporter fusion, and phenotypic experiments were conducted using L. monocytogenes prfA*, prfA* ΔsigB, ΔprfA, and ΔprfA ΔsigB strains. Genome-wide transcript profiling and qRT-PCR showed that in the presence of active PrfA (PrfA*), σB is responsible for reduced expression of the PrfA regulon. σB-dependent modulation of PrfA regulon expression reduced the cytotoxic effects of a PrfA* strain in HepG2 cells, highlighting the functional importance of regulatory interactions between PrfA and σB. The emerging model of the role of σB in regulating overall PrfA activity includes a switch from transcriptional activation at the P2 prfA promoter (e.g., in extracellular bacteria when PrfA activity is low) to posttranscriptional downregulation of PrfA regulon expression (e.g., in intracellular bacteria when PrfA activity is high).

scholarly journals Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L.

2021 ◽  
Vol 22 (14) ◽  
pp. 7396
Author(s):  
Manu Kumar ◽  
Bhagwat Singh Kherawat ◽  
Prajjal Dey ◽  
Debanjana Saha ◽  
Anupama Singh ◽  
...  
Keyword(s):  
Gene Family ◽  
Auxin Transport ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
Stress Conditions ◽  
Biotic And Abiotic Stress ◽  
Developmental Processes ◽  
Qrt Pcr ◽  
Genome Wide

PIN-FORMED (PIN) genes play a crucial role in regulating polar auxin distribution in diverse developmental processes, including tropic responses, embryogenesis, tissue differentiation, and organogenesis. However, the role of PIN-mediated auxin transport in various plant species is poorly understood. Currently, no information is available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the PIN gene family in wheat to understand the evolution of PIN-mediated auxin transport and its role in various developmental processes and under different biotic and abiotic stress conditions. In this study, we performed genome-wide analysis of the PIN gene family in common wheat and identified 44 TaPIN genes through a homology search, further characterizing them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses led to the classification of TaPIN genes into seven different groups, providing evidence of an evolutionary relationship with Arabidopsis thaliana and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, transmembrane domains, and three-dimensional (3D) structure were also examined using various computational approaches. Cis-elements analysis of TaPIN genes showed that TaPIN promoters consist of phytohormone, plant growth and development, and stress-related cis-elements. In addition, expression profile analysis also revealed that the expression patterns of the TaPIN genes were different in different tissues and developmental stages. Several members of the TaPIN family were induced during biotic and abiotic stress. Moreover, the expression patterns of TaPIN genes were verified by qRT-PCR. The qRT-PCR results also show a similar expression with slight variation. Therefore, the outcome of this study provides basic genomic information on the expression of the TaPIN gene family and will pave the way for dissecting the precise role of TaPINs in plant developmental processes and different stress conditions.

scholarly journals Identification of Novel PHD-Finger Genes in Pepper by Genomic Re-Annotation and Comparative Analyses

2021 ◽  
Author(s):  
Ji Yoon Guk ◽  
Min Jeong Jang ◽  
Seungill Kim
Keyword(s):  
Gene Family ◽  
Phd Finger ◽  
Functional Studies ◽  
Qrt Pcr ◽  
Genome Wide ◽  
Zinc Finger Genes ◽  
Plant Homeodomain ◽  
Integrated Domains ◽  
Functional Analyses

Abstract BackgroundThe plant homeodomain (PHD)-finger gene family that belongs to zinc-finger genes, plays important roles in epigenetics by regulating gene expression in eukaryotes. However, inaccurate annotation of PHD-finger genes hinders further downstream comparative, evolutionary, and functional studies.ResultsWe performed genome-wide re-annotation in Arabidopsis, rice, pepper, potato, and tomato to better understand the role of PHD-finger genes in these species. Our investigation identified 875 PHD-finger genes, of which 225 (26% of total) were newly identified, including 57 (54%) novel PHD-finger genes in pepper. The PHD-finger genes of the five plant species have various integrated domains that may be responsible for the diversification of structures and functions of these genes. Evolutionary analyses suggest that PHD-finger genes were expanded recently by lineage-specific duplication, especially in pepper and potato, resulting in diverse repertoires of PHD-finger genes among the species. We validated the expression of six newly identified PHD-finger genes in pepper with qRT-PCR. Transcriptome analyses suggest potential functions of PHD-finger genes in response to various abiotic stresses in pepper.ConclusionsOur data, including the updated annotation of PHD-finger genes, provide useful information for further evolutionary and functional analyses to better understand the roles of the PHD-finger gene family in pepper.

scholarly journals Integrative Transcriptome Analysis Define Novel lncRNA Potential Regulation Function through SLC22A7 in Yak Liver

2021 ◽  
Author(s):  
Wei Xia ◽  
Fang Fu ◽  
Li Wang ◽  
Xiaolin Luo ◽  
Jiuqiang Guan
Keyword(s):  
Correlation Analysis ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Qrt Pcr ◽  
The People ◽  
Anion Transporter ◽  
Plateau Area ◽  
Regulation Function

Abstract Background: The yak (Bos grunniens) is a crucial resource to supply meat and milk to the people localized in Qinghai-Tibetan plateau area. To identify lncRNAs regulating metabolism in yak, this work adopted transcriptome method to simultaneously profile mRNAs and lncRNAs of liver in yak under three representative age (LD: Liver 1 Day, LM: Liver 15 Months, LY: Liver 5 Years) conditions.Result: Of 288 differentially expressed lncRNAs, function-oriented selection yield 88 regulated metabolically related lncRNAs that were differentially expressed at least two age conditions. These lncRNAs predicted by lncRNA-mRNA correlation analysis to function in various aspects of metabolism. Selected regulations of liver metabolically related lncRNAs were further verified by qRT-PCR. Furthermore, one novel LncRNA were selected to validate its function and result showed it potentially regulated SLC22A7, a well-known organic anion transporter.Conclusion: Combining high throughput RNA-seq screening screens, bioinformatics predictions, lncRNA-mRNA correlation analysis and qRT-PCR analysis, this study supports that a class of lncRNAs function as important metabolic regulators and establishes a foundation for further investigating the role of lncRNAs in yak.

Public Administration in the Islamic World: Considering the Importance of Religion, its Values and Culture

2019 ◽  
Vol 19 (2) ◽  
pp. 23-38
Author(s):  
Daniel Hummel
Keyword(s):  
Public Administration ◽  
Core Values ◽  
Comparative Perspective ◽  
Religious Values ◽  
Future Research ◽  
Islamic World ◽  
Salient Region ◽  
The People ◽  
Comparative Public Administration

A small but growing area of public administration scholarship appreciates the influence of religious values on various aspects of government. This appreciation parallels a growing interest in comparative public administration and indigenized forms of government which recognizes the role of culture in different approaches to government. This article is at the crossroads of these two trends while also considering a very salient region, the Islamic world. The Islamic world is uniquely religious, which makes this discussion even more relevant, as the nations that represent them strive towards legitimacy and stability. The history and core values of Islam need to be considered as they pertain to systems of government that are widely accepted by the people. In essence, this is being done in many countries across the Islamic world, providing fertile grounds for public administration research from a comparative perspective. This paper explores these possibilities for future research on this topic.

Analisis Pengembalian Kredit Terhadap Profitabilitas Pada PT Bank Sumut Cabang Gunungsitoli

Owner ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 160
Author(s):  
Victorinus Laoli
Keyword(s):  
Data Collection ◽  
Positive Influence ◽  
Business Continuity ◽  
Working Capital ◽  
The People ◽  
Data Collection Technique

One manifestation of the important role of banking in a region, as implemented by PT Bank Sumut, Gunungsitoli Branch, is to distribute loans for investment, consumption and working capital for the people in the area. The purpose of providing credit for banks is the return of credit that earns interest and can increase income to finance activities and business continuity. From the results of research conducted with this data collection technique, it shows that PT Bank Sumut has a number of loans from 2009 to 2014 which each year rises. From this study, it is also known that the rate of credit repayment has a positive influence on the level of profitability.

INTERPRETATION OF SINO-RUSSIAN CULTURE AND HUMANITIES COOPERATION IN THE NEW ERA

2020 ◽  
Vol 10 (3) ◽  
pp. 186-193
Author(s):  
REN YANYAN ◽  
Keyword(s):  
Current Status ◽  
Human Relations ◽  
New Era ◽  
Russian Culture ◽  
The People ◽  
Starting Point ◽  
Cooperative Partnership ◽  
Belt And Road ◽  
The Belt And Road

The friendship between nations lies in the mutual affinity of the people, and the people’s affinity lies in the communion of hearts. The cultural and humanities cooperation between China and Russia has a long history. In recent years, under the role of the“Belt and Road” initiative, the SCO, and the Sino-Russian Humanities Cooperation Committee, Sino-Russian culture and humanities cooperation has continued to deepen. Entering a new era, taking the opportunity to promote Sino-Russian relations into a “new era China-Russia comprehensive strategic cooperative partnership”, the development of human relations between the two countries has entered a new historical starting point, while also facing a series of problems and challenges. This article is based on the current status of Sino-Russian human relations in the new era, interprets the characteristics of Sino-Russian human relations in the new era, analyzes the problems and challenges of Sino-Russian human relations in the new era, and tries to propose solutions and solutions with a view to further developing Sino-Russian cultural and humanities relations in the new era. It is a useful reference, and provides a reference for future related research, and ultimately helps the Sino-Russian cultural and humanities relations in the new era to be stable and far-reaching.

CULTURE OF THE RUSSIAN WORLD - A SPIRITUAL SLOGAN FACTOR OF VICTORY IN THE GREAT PATRIOTIC WAR

2020 ◽  
Vol 10 (2) ◽  
pp. 169-173
Author(s):  
VLADIMIR KSENOFONTOV ◽  
Keyword(s):  
Emotional Expression ◽  
Front Line ◽  
Soviet People ◽  
The People ◽  
Great Patriotic War ◽  
Red Army ◽  
Art Activities ◽  
Artistic Images ◽  
Great Victory

The article reveals the essence and specificity of culture as an important component of the spiritual factor of victory. Special attention is paid to the characteristics of fiction. The article substantiates the moral and aesthetic impact on the consciousness of defenders of the Motherland, such works of art as“They fought for the Motherland”,“Leningrad poem”,“Russian character”,“Invasion”, etc. The article describes the significant role of theatrical art, which reveals the moral values of the people and Soviet soldiers. This is reflected in such plays as: “the Front”; “the Guy from our city”; “Once upon a time”, etc. The article substantiates the important role of the spiritual influence of cinema on Soviet people. This influence was realized through artistic images of selfless service to the Motherland, loyalty to military duty. Among these films: “Two fighters”, “Wait for me”, “Front-line friends”. During the war, as the article emphasizes, an important component of the spiritual factor of victory was the musical art. Activities in this area of culture famous musicians:B. Astafiev, S. Prokofiev, D. Shostakovich, A. Alexandrov, V. Soloviev-Sedoy, and others, was implemented in operas, symphonies, cantatas and songs, which by their nature emotional expression differed Patriotic and epic strength. The purpose of the research : to reveal the axiological components, culture of the Russian world, as important components, spiritual factor during the great Patriotic war. Conclusions : the culture of the Russian world at various stages of the great Patriotic War, through a variety of means and forms, actively mobilized all Soviet people to defend the Motherland and defeat Nazi Germany. The spiritual culture of our country and its types, in the course of functioning, during the war, clearly and expressively revealed the idea of patriotism, courage, bravery and heroism, and encouraged the Soviet people, the soldiers of the red Army, to achieve a great Victory.

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