scholarly journals Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

2020 ◽  
Author(s):  
Ana Carolina Piva de Oliveira ◽  
Fernanda Lopes de Figueiredo ◽  
Thiago A Gonçalves ◽  
Cesar RF Terrasan ◽  
Geizecler Tomazetto ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Eucalyptus Grandis ◽  
Wood Decay ◽  
Brown Rot ◽  
Enzymatic Mechanism ◽  
Sugarcane Straw ◽  
Laetiporus Sulphureus ◽  
Omics Analysis ◽  
Specific Proteins ◽  
Hydrolytic Mechanism

Abstract Background: Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant substrates. Brown-rot strains produce carbohydrate-active enzymes involved in the degradation of lignocellulosic materials, along with a non-enzymatic mechanism via Fenton reaction. Differences in the lignocellulose metabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 in the degradation of lignocellulosic by-products derived from sugarcane and Eucalyptus. Results: To evidence the oxidative-hydrolytic mechanism, the Laetiporus sulphureus ATCC 52600 genome was sequenced and the response to lignocellulosic substrates was analyzed by transcriptomics and proteomics. The transcriptomic profile in response to a short cultivation period on in natura sugarcane bagasse revealed 128 out of 12,802 upregulated transcripts. The high upregulated transcripts included a set of redox enzymes along with hemicellulases. The exoproteome produced in response to extended time cultivation on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus (from Eucalyptus grandis) revealed 121 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated proteins relative to glucose. The secretome produced on sugarcane bagasse was evaluated in the saccharification of pretreated sugarcane straw by supplementing a commercial cocktail. Additionally, growth analysis revealed that L. sulphureus ATCC 52600 has higher efficiency to assimilate glucose than other mono- and disaccharides.Conclusion: This study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative-hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.

scholarly journals Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Fernanda Lopes de Figueiredo ◽  
Ana Carolina Piva de Oliveira ◽  
Cesar Rafael Fanchini Terrasan ◽  
Thiago Augusto Gonçalves ◽  
Jaqueline Aline Gerhardt ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Wood Decay ◽  
Brown Rot ◽  
Lignocellulose Degradation ◽  
Phylogenomic Analysis ◽  
Late Response ◽  
Redox Enzymes ◽  
Sugarcane Straw ◽  
Laetiporus Sulphureus ◽  
Omics Analysis

Abstract Background Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydrate-active enzymes and non-enzymatic Fenton mechanism. Differences in the lignocellulose catabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 for lignocellulose degradation. Results The genome of Laetiporus sulphureus ATCC 52600 was sequenced and phylogenomic analysis supported monophyletic clades for the Order Polyporales and classification of this species within the family Laetiporaceae. Additionally, the plasticity of its metabolism was revealed in growth analysis on mono- and disaccharides, and polysaccharides such as cellulose, hemicelluloses, and polygalacturonic acid. The response of this fungus to the presence of lignocellulosic substrates was analyzed by transcriptomics and proteomics and evidenced the occurrence of an integrated oxidative–hydrolytic metabolism. The transcriptomic profile in response to a short cultivation period on sugarcane bagasse revealed 125 upregulated transcripts, which included CAZymes (redox enzymes and hemicellulases) as well as non-CAZy redox enzymes and genes related to the synthesis of low-molecular-weight compounds. The exoproteome produced in response to extended cultivation time on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus revealed 112 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated CAZymes. The secretome induced for 7 days on sugarcane bagasse, representative of the late response, was applied in the saccharification of hydrothermally pretreated grass (sugarcane straw) and softwood (pine) by supplementing a commercial cocktail. Conclusion This study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative–hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.

scholarly journals Chicken of the Woods (Laetiporus sulphureus species complex)

EDIS ◽  
2020 ◽  
Vol 2020 (5) ◽  
Author(s):  
Brianna Benitez ◽  
Claudia Paez ◽  
Matthew Smith ◽  
Jason A. Smith
Keyword(s):  
Plant Pathology ◽  
Natural Products ◽  
Species Complex ◽  
Wood Decay ◽  
Brown Rot ◽  
Human Consumption ◽  
Decay Fungi ◽  
Laetiporus Sulphureus ◽  
Wood Decay Fungi ◽  
The Common

Species in the Laetiporus sulphureus species complex, also known as “chicken of the woods” mushrooms, are wood-decay fungi that cause brown rot within the heartwood of their tree hosts. The common name “chicken of the woods” is given to some species in this group because they are tasty edible mushrooms. Several Laetiporus species have been harvested to use as food colorants, to dye natural products such as wool, and for human consumption. This new 4-page publication of the UF/IFAS Plant Pathology Department, written by Brianna Benitez, Claudia A. Paez, Matthew E. Smith, and Jason A. Smith, describes these fungi as well as their ecology, management, and potential edibility.https://edis.ifas.ufl.edu/pp358

The influence of the elemental and structural chemical composition on the ash fusibility of sugarcane bagasse and sugarcane straw

Fuel ◽  
2021 ◽  
Vol 304 ◽  
pp. 121404
Author(s):  
Kelly Roberta de Palma ◽  
Edson Tomaz ◽  
Antonio Soria-Verdugo ◽  
Maria Aparecida Silva
Keyword(s):  
Chemical Composition ◽  
Sugarcane Bagasse ◽  
Sugarcane Straw ◽  
Ash Fusibility

Composition and antifungal activity of balsam from Liquidambar formosana Hance

Holzforschung ◽  
2013 ◽  
Vol 67 (3) ◽  
pp. 345-351 ◽  
Author(s):  
Shih-Chang Chien ◽  
Jun-Hong Xiao ◽  
Yen-Hsueh Tseng ◽  
Yueh-Hsiung Kuo ◽  
Sheng-Yang Wang
Keyword(s):  
Antifungal Activity ◽  
Volatile Compounds ◽  
Tree Species ◽  
Brown Rot ◽  
Betulonic Acid ◽  
White Rot ◽  
Spectroscopic Techniques ◽  
Laetiporus Sulphureus ◽  
Lenzites Betulina

Abstract Formosan sweet gum (Liquidambar formosana Hance) is a tree species endemic in Taiwan. In this study, the composition of balsam from L. formosana has been determined by several chromatographic and spectroscopic techniques. Among the 26 compounds identified, three new triterpenoids were detected, namely, 2α,3α-dihydroxyolean-12-en-28-al (1), 3α-hydroxyolean-12-en-30-ol (2), and 3α-hydroxyolean-2-oxo-12-en-28-al (3). The most abundant volatile compounds were β-caryophyllene (22.7%), α-pinene (23.3%), and β-pinene (19.6%), and the most abundant nonvolatile compounds were 3α,25-dihydroxyolean-12-en-28-oic acid (12, 19.1%), oleanonic aldehyde (9, 14.0%), and betulonic acid (15, 13.4%). The compounds 3α,25-dihydroxyolean-12-en-28-oic acid and bornyl cinnamate were found to be inhibitory for white rot (Lenzites betulina) and brown rot (Laetiporus sulphureus) fungi.

Climate influences the effect of fungal decay type on regeneration of Picea jezoensis var. hondoensis seedlings on decaying logs

2020 ◽  
Vol 50 (1) ◽  
pp. 73-79
Author(s):  
Yu Fukasawa ◽  
Yoko Ando ◽  
Satoshi N. Suzuki ◽  
Mineaki Aizawa ◽  
Daisuke Sakuma
Keyword(s):  
Wood Decay ◽  
Brown Rot ◽  
Soft Rot ◽  
Climatic Conditions ◽  
White Rot ◽  
Seedling Density ◽  
Picea Jezoensis ◽  
Central Japan ◽  
Seedling Regeneration ◽  
Mountainous Regions

Hondo spruce (Picea jezoensis var. hondoensis (Mayr) Rehder)) is separately distributed among several mountainous regions in central Japan as remnant populations of the last glacial period. To identify factors that affect Hondo spruce seedling regeneration on decaying logs, we investigated the relationships between climatic conditions, log properties, including decay type by fungi, and Hondo spruce seedling density on logs using data from seven subalpine Hondo spruce forests in central Japan. The results showed that the presence of soft rot was associated with higher seedling density, and the effect of brown rot in sapwood and white rot in heartwood on the predicted number of spruce seedlings on logs switched from positive to negative with increasing temperature and precipitation. Because soft rot occurs under humid conditions, the use of forest management techniques that increase the number of logs with soft rot in sapwood (e.g., by keeping the forest floor moist) are recommended for the sustainable regeneration of Hondo spruce. However, the relationships between wood decay type and seedling regeneration can also be affected by climate condition and thus are more complex than previously thought.

Variation in germination percentage of basidiospores collected successively from wood decay fungi in culture

1983 ◽  
Vol 61 (1) ◽  
pp. 171-173 ◽  
Author(s):  
E. L. Schmidt ◽  
D. W. French
Keyword(s):  
Spore Germination ◽  
White Rot Fungi ◽  
Wood Decay ◽  
Brown Rot ◽  
Germination Percentage ◽  
White Rot ◽  
Malt Extract ◽  
Decay Fungi ◽  
Brown Rot Fungi ◽  
Wood Decay Fungi

Successive collections of basidiospores, produced in culture from the same hymenial areas of four species of wood decay fungi, were tested for spore germination percentage on malt extract agar under controlled conditions. Spores from white rot fungi retained high germination levels after 5 weeks of spore production, but germination averages for brown rot fungi decreased by more than 50%. Such variation should be considered in wood pathology research using spore germination bioassay.

scholarly journals Laccase and Its Role in Production of Extracellular Reactive Oxygen Species during Wood Decay by the Brown Rot Basidiomycete Postia placenta

2010 ◽  
Vol 76 (7) ◽  
pp. 2091-2097 ◽  
Author(s):  
Dongsheng Wei ◽  
Carl J. Houtman ◽  
Alexander N. Kapich ◽  
Christopher G. Hunt ◽  
Daniel Cullen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Identification ◽  
Reactive Oxygen ◽  
Wood Decay ◽  
Brown Rot ◽  
Order Rate Constant ◽  
Spectrometric Analysis ◽  
Alternative Mechanism ◽  
Oxygen Species ◽  
Postia Placenta

ABSTRACT Brown rot basidiomycetes initiate wood decay by producing extracellular reactive oxygen species that depolymerize the structural polysaccharides of lignocellulose. Secreted fungal hydroquinones are considered one contributor because they have been shown to reduce Fe3+, thus generating perhydroxyl radicals and Fe2+, which subsequently react further to produce biodegradative hydroxyl radicals. However, many brown rot fungi also secrete high levels of oxalate, which chelates Fe3+ tightly, making it unreactive with hydroquinones. For hydroquinone-driven hydroxyl radical production to contribute in this environment, an alternative mechanism to oxidize hydroquinones is required. We show here that aspen wood undergoing decay by the oxalate producer Postia placenta contained both 2,5-dimethoxyhydroquinone and laccase activity. Mass spectrometric analysis of proteins extracted from the wood identified a putative laccase (Joint Genome Institute P. placenta protein identification number 111314), and heterologous expression of the corresponding gene confirmed this assignment. Ultrafiltration experiments with liquid pressed from the biodegrading wood showed that a high-molecular-weight component was required for it to oxidize 2,5-dimethoxyhydroquinone rapidly and that this component was replaceable by P. placenta laccase. The purified laccase oxidized 2,5-dimethoxyhydroquinone with a second-order rate constant near 104 M−1 s−1, and measurements of the H2O2 produced indicated that approximately one perhydroxyl radical was generated per hydroquinone supplied. Using these values and a previously developed computer model, we estimate that the quantity of reactive oxygen species produced by P. placenta laccase in wood is large enough that it likely contributes to incipient decay.

NMR Structural Characterization ofQuercus alba(White Oak) Degraded by the Brown Rot Fungus,Laetiporus sulphureus

2010 ◽  
Vol 30 (1) ◽  
pp. 61-85 ◽  
Author(s):  
Amanda B. Koenig ◽  
Rachel L. Sleighter ◽  
Elodie Salmon ◽  
Patrick G. Hatcher
Keyword(s):  
Structural Characterization ◽  
Brown Rot ◽  
White Oak ◽  
Laetiporus Sulphureus ◽  
Brown Rot Fungus

scholarly journals CARACTERIZAÇÃO DE BIOMASSAS PARA A BRIQUETAGEM

FLORESTA ◽  
2015 ◽  
Vol 45 (4) ◽  
pp. 713 ◽  
Author(s):  
Diego Aleixo Silva ◽  
Gabriela Tami Nakashima ◽  
João Lúcio Barros ◽  
Alessandra Luzia Da Roz ◽  
Fabio Minoru Yamaji
Keyword(s):  
Particle Size ◽  
Moisture Content ◽  
Sugarcane Bagasse ◽  
Saccharum Officinarum ◽  
Ash Content ◽  
Heating Value ◽  
Pine Sawdust ◽  
High Heating Value ◽  
Sugarcane Straw ◽  
High Heating

O objetivo deste trabalho foi caracterizar a produção de briquetes feita a partir de quatro diferentes biomassas residuais. Foram utilizados os resíduos de serragem de Eucalyptus sp, serragem de Pinus sp, bagaço de cana-de-açúcar (Saccharum officinarum L.) e palha de cana-de-açúcar. Os resíduos foram tratados para que obtivessem 12% de umidade e uma granulometria inferior a 1,70 mm. Foram produzidos 15 briquetes para cada um dos quatro tratamentos. A pressão utilizada foi de 1250 kgf.cm-2 durante 30 segundos. Os briquetes obtiveram densidades que oscilaram 0,88 a 1,11 g.cm-3. Isto representou uma faixa de 5 a 14 vezes a menos de ocupação de volume para uma mesma quantidade de massa. O poder calorifico foi de 19.180 J.kg-1 e 20.315 J.kg-1 para as serragens de eucalipto e pinus respectivamente. Para o bagaço e palha de cana os valores foram de 18.541 J.kg-1 e 15.628 J.kg-1. A palha da cana-de-açúcar apresentou um teor de cinzas de 12%. As expansões dos tratamentos oscilaram 4 a 9% e as resistências mecânicas variaram de 1,215 MPa à 0,270 MPa. Todos os briquetes se mostraram resistentes para um empilhamento superior a 10 m de altura. O procedimento adotado pode ajudar a diminuir o espaço de estocagem e de transporte. AbstractThis research aims to characterize the production of briquettes from four different biomasses. We used residues such as Eucalyptus sp sawdust, Pinus sp sawdust , sugarcane bagasse (Saccharum officinarum L.) and sugarcane straw. The residues were treated to obtain 12% moisture content and particle size less than 1.70 mm. We produced 15 briquettes for each treatment. The pressure used was 1250 kgf.cm-2 for 30 seconds. The briquettes obtained densities ranged from 0.88 to 1.11 g.cm-3. This represented a range of 5 to 14 times less volume occupancy for the same amount of mass. The high heating value (HHV) was 19,180 J.kg-1 and 20,315 J.kg-1 for eucalyptus and pine sawdust respectively. The HHV for the bagasse was 18,541 J.kg-1 and for straw was 15,628 J.kg-1. The straw presented an ash content of 12%. The expansions of the treatments ranged 4 to 9% and mechanical resistances ranging from 1,215 MPa to 0,270 MPa. All briquettes were resistant to a higher stacking to 10 m high. The methods can help to decrease the space of storage and transport.Keywords: Waste; biofuel; energy; compression; stacking.

Changes in Carbon Sequestration in Wood During Decay by Brown- and White-Rot Fungi

2020 ◽  
Vol 14 (3) ◽  
pp. 414-419
Author(s):  
Huadong Xu ◽  
Jiedong Wei ◽  
Yanan Di ◽  
Ruixia Qin ◽  
Zonglin Zhen
Keyword(s):  
Carbon Sequestration ◽  
Mass Loss ◽  
Decay Time ◽  
Carbon Emission ◽  
Wood Decay ◽  
Brown Rot ◽  
White Rot ◽  
Korean Pine ◽  
Decay Test ◽  
The Absolute

Wood decay is a releasing process of carbon fixed in the wood. The study on carbon sequestration change caused by decay can provide a theoretical basis for wood preservation and utilization. At present, there are few reports on decay influence on wood carbon emission and no corresponding quantitative data. Therefore, one broad-leaved species, Poplar, and one coniferous species, Korean pine, were selected as the research object, and brown rot fungus (Gloeephyllum trabeum) and white rot fungus (Coriolus versicolor) were used to conduct accelerated decay test on wood samples in the laboratory. During decay, specimens were taken out in different periods to measure chemical properties, mass loss and carbon sequestration. The influence of decay time on carbon sequestration, chemical component and mass loss were then analyzed and the change rule of carbon sequestration were finally studied. The results showed that with increasing decay time, the relative carbon sequestration content of wood affected by different types rot fungi decreased, which was consistent with the change rule of mass loss, indicating that decay would lead to a loss of wood mass and affect its carbon sequestration. However, the absolute carbon sequestration (measured value of carbon sequestration) after brown rot treatment did not decrease but increased slightly, which was different from previous expectation. According to the analysis, with increasing brown rot time, the absolute content and proportion of lignin in wood samples increased slightly, while the corresponding value of holocellulose (including α-cellulose and hemicellulose) decreased significantly. The carbon content of lignin per unit mass is higher than that of holocellulose (Poplar 64.08% > 37.38%; Korean pine 66.37% > 35.94%), resulting in absolute carbon sequestration in wood increases instead of decreases. In conclusion, the change of lignin proportion during the process of brown rot is the decisive factor affecting the change of absolute carbon sequestration. This study focused on two aspects of wood decay and wood carbon sequestration, systematically analyzed the change rule and internal mechanism of wood carbon sequestration with the increase of wood decay degree, and accumulated basic data for wood carbon emission reduction and wood prevention.

Sign in / Sign up

Export Citation Format

Share Document