Manganese Sulphide Nano Rods grown inside Acacia Senegal Gum Hydrogels; A Biocompatible Nanofabrication for Enhanced Biological activities
Abstract The present work demonstrates the synthesis and biological properties of novel manganese sulphide (MnS) hydrogels using acacia Senegal gum (ASG) as a natural biopolymer and divinyl sulfone (DS) as a cross-linker via in situ reduction method. Acacia Senegal Gum hydrogel p(ASG) and manganese sulphide (MnS) nano-rods fabricated hydrogels p(ASG)-MnS hydrogels were then characterized through various techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray diffraction (XRD), fourier transform infrared (FTIR) spectroscopy, and thermogravimetric analysis (TGA). Biomedical investigation of pristine and p(ASG)-MnS was carried out by evaluating their biocompatibility, antioxidant and antidiabetic activities. Both the pristine and hybrid hydrogel shown excellent biocompatibility with 0.759 % hemolysis for p(ASG) and 2.386 % haemolytic activity for p(ASG)-MnS at the highest tested dose of 400 µg/mL. Furthermore, p (ASG)-MnS displayed remarkable antioxidant activities as evaluated by multiple antioxidant assays. The p(ASG)-MnS exhibit better DPPH and ABTS scavenging activities of 66.91±0.22 (%) and 98.40±0.58 (TEAC), respectively. On the other hand, p (ASG) showed 7.5%±0.58 (%) FRSA and 4.40±0.28 (TEAC), activity. Similarly, total antioxidant capacity (TAC) and total reducing power (TRP) values for p (ASG)-MnS were 184.32±2.3 (µg AAE/mg) and 179.83±0.1.2 (µg AAE/mg), respectively thus proving the considerable antioxidant properties of the hybrid hydrogels. The antidiabetic activity of p (ASG) and p(ASG)-MnS hydrogels were examined by determining their alpha-amylase inhibition potential. The p(ASG)-MnS displayed average alpha-amylase with numerical value 16.7±1.4 (%) as compared to p(ASG) with very weak inhibition potential of 4.18±0.98 (%). To conclude, p(ASG)-MnS hydrogel have excellent biocompatibility and antioxidant potential and reasonable antidiabetic activity as compare to pristine p(ASG).