scholarly journals Influence of the treatment with the antineoplastic agents 5-fluorouracil and Cisplatin on the severity of experimental periodontitis in rats

2021 ◽  
Author(s):  
vivian novaes ◽  
Edilson Ervolino ◽  
Giovani Lopes Fernandes ◽  
Clara Possarle Cunha ◽  
Leticia Helena Theodoro ◽  
...  
Keyword(s):  
Antineoplastic Agents ◽  
Alveolar Bone ◽  
Physiological Saline ◽  
Male Rats ◽  
Lower Percentage ◽  
Periodontal Tissues ◽  
Periodontal Breakdown ◽  
Physiological Saline Solution ◽  
Treatment Data ◽  
Experimental Periodontitis

Abstract Purpose The determination on how antineoplastic agents interfere on the progression of periodontitis is critical for improvement and even development of novel therapeutic approaches for periodontal management. This study evaluated the influence of chemotherapy with 5-fluorouracil (5-FU) or cisplatin (CIS) on healthy periodontal tissues and on the progression of experimental periodontitis (EP). Methods One-hundred-forty-four male rats were divided into six groups (n = 24). Each group was treated with physiological saline solution (PSS) 0.9%, 5-FU or CIS. Experimental periodontitis (EP) was induced by ligature placement. Animals were euthanized at 7, 15 and 30 days after treatment. Data were statistically analyzed (p ≤ 0.05). Results The groups with EP and treated with 5-FU or CIS showed lower percentage of bone volume in the furcation region and higher percentage of alveolar bone loss, higher number of TRAP-positive cells and lower number of PCNA-positive cells when compared group with EP and treated with PSS (p ≤ 0.05). Groups with EP and treated with 5-FU or CIS showed high immunolabelling pattern of RANKL, TNF-α, IL-1β, moderate of BAX and low of HIF-1α. Histological analysis showed severe tissue breakdown in the groups with EP and treated with 5-FU or CIS. Conclusions Chemotherapy with antineoplastic agents 5-FU and CIS increasing the intensity and duration of the inflammation; and compromising tissue repair by reduction in cellular and vascular turnover. The more severe periodontal breakdown was caused by 5-FU.

scholarly journals Influence of immunosuppression on the progression of experimental periodontitis and on healthy periodontal tissue: A rat in vivo study

2021 ◽  
Vol 15 (2) ◽  
pp. 94-99
Author(s):  
Juliano Milanezi de Almeida ◽  
Henrique Rinaldi Matheus ◽  
Luiz Guilherme Fiorin ◽  
Elisa Mara Abreu Furquim ◽  
David Jonathan Rodrigues Gusman
Keyword(s):  
Saline Solution ◽  
Alveolar Bone ◽  
Physiological Saline ◽  
Male Rats ◽  
Time Intervals ◽  
Subcutaneous Injections ◽  
Physiological Saline Solution ◽  
Experimental Periodontitis ◽  
Post Hoc

Background. The potent anti-inflammatory and immunosuppressive properties of glucocorticoids (GCs) might influence the progression of some disorders, such as periodontitis. Hence, this study aimed to investigate the influence of dexamethasone (DEX) on the alveolar bone loss (ABL) of healthy and periodontally compromised molars in rats. Methods. Thirty male rats were randomly assigned to two groups: physiological saline solution (PSS) and DEX. The animals received subcutaneous injections of either 0.5 mL of PSS) (group PSS) or 2 mg/kg of DEX (group DEX) from one day before experimental periodontitis (EP) induction until euthanasia. EP was induced through ligature placement around the mandibular lower first molars at day 0. Contralateral molars remained unligated. Ten animals per period were euthanized on days 3, 7, and 14. Morphometric analysis was performed to access the ABL. Data were statistically analyzed with ANOVA followed by post hoc Tukey tests (P≤0.05). Results. Higher ABL was observed in both groups on days 7 and 14 than on day 3 (P≤0.05). Concerning periodontitis, higher ABL was observed in group DEX on days 3, 7, and 14 days than group PSS at the same time intervals (P≤0.05). Also, even in the contralateral unligated molars, group DEX exhibited higher ABL on days 3, 7, and 14 days than group PSS at the same time intervals (P≤0.05). Conclusions. Collectively, it can be concluded that DEX aggravates EP and induces spontaneous ABL in the healthy periodontium.

scholarly journals The effect of alcohol consumption on periodontal bone support in experimental periodontitis in rats

2006 ◽  
Vol 14 (6) ◽  
pp. 443-447 ◽  
Author(s):  
Daniela Martins de Souza ◽  
Lucilene Hernandes Ricardo ◽  
Marcela de Almeida Prado ◽  
Fernanda de Almeida Prado ◽  
Rosilene Fernandes da Rocha
Keyword(s):  
Alcohol Consumption ◽  
Alveolar Bone ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
High Concentration ◽  
Experimental Periodontitis ◽  
Distal Aspect ◽  
Caloric Consumption ◽  
Mandibular First Molar

OBJECTIVE: The aim of this study was to evaluate the effect of the alcohol consumption on the periodontal bone support (PBS) in experimental periodontitis in rats. MATERIALS AND METHODS: Sixty-three male rats were divided into seven groups: G1 (control); G2 (10% ethanol); G3 (nutritional control of G2); G4 (20% ethanol); G5 (nutritional control of G4); G6 (30% ethanol) and G7 (nutritional control of G6). The groups G3, G5 and G7 received controlled diets with equivalent caloric amounts to those consumed in G2, G4 and G6 respectively, with the ethanol replaced by sucrose. After anesthesia, ligatures were installed around the mandibular first molar, leaving the contralateral teeth unligated. After 8 weeks, the rats were killed and their mandibles were radiographed to measure the percentage of PBS on the distal aspect. RESULTS: The intragroup analyses showed that presence of ligatures induced periodontitis (p<0.05). Unligated groups did not show significant differences among the percentages of PBS (p=0.1969). However, in ligated groups the rats that received alcohol (G2:48.71%±3.88; G4:47.66%±2.54; G6:47.32%±3.24) and the nutritional control group associated with a high concentration of ethanol (G7:47.40%±3.24) presented a significantly lower percentage of PBS than the other groups (G1:52.40%±2.75; G3:52.83%±2.41; G5:50.85%±4.14). CONCLUSIONS: These results demonstrated that alcohol consumption in rats may result in a direct effect on alveolar bone loss and increased development of periodontitis. In addition, they suggest that heavy caloric consumption of ethanol may also present an indirect effect on periodontal tissue as a consequence of malnutrition.

scholarly journals Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hui Yu ◽  
Tianyi Zhang ◽  
Haibin Lu ◽  
Qi Ma ◽  
Dong Zhao ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Bone Volume ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Periodontal Tissues ◽  
Gingival Epithelial Cells ◽  
Trap Staining ◽  
Experimental Periodontitis ◽  
Stimulating Factor

Abstract Background Granulocyte colony-stimulating factor (G-CSF) is an important immune factor that mediates bone metabolism by regulating the functions of osteoclasts and osteoblasts. Bone loss is a serious and progressive result of periodontitis. However, the mechanisms underlying the effects of G-CSF on periodontal inflammation have yet not been completely elucidated. Here, we examined whether an anti-G-CSF antibody could inhibit bone resorption in a model of experimental periodontitis and investigated the local expression of G-CSF in periodontal tissues. Methods Experimental periodontitis was induced in mice using ligatures. The levels of G-CSF in serum and bone marrow were measured; immunofluorescence was then performed to analyze the localization and expression of G-CSF in periodontal tissues. Mice with periodontitis were administered anti-G-CSF antibody by tail vein injection to assess the inhibition of bone resorption. Three-dimensional reconstruction was performed to measure bone destruction‐related parameters via micro-computed tomography analysis. Immunofluorescence staining was used to investigate the presence of osteocalcin-positive osteoblasts; tartrate-resistant acid phosphatase (TRAP) staining was used to observe osteoclast activity in alveolar bone. Results The level of G-CSF in serum was significantly elevated in mice with periodontitis. Immunofluorescence analyses showed that G-CSF was mostly expressed in the cell membrane of gingival epithelial cells; this expression was enhanced in the periodontitis group. Additionally, systemic administration of anti-G-CSF antibody significantly inhibited alveolar bone resorption, as evidenced by improvements in bone volume/total volume, bone surface area/bone volume, trabecular thickness, trabecular spacing, and trabecular pattern factor values. Immunofluorescence analysis revealed an enhanced number of osteocalcin-positive osteoblasts, while TRAP staining revealed reduction of osteoclast activity. Conclusions G-CSF expression levels were significantly up-regulated in the serum and gingival epithelial cells. Together, anti-G-CSF antibody administration could alleviates alveolar bone resorption, suggesting that G-CSF may be one of the essential immune factors that mediate the bone loss in periodontitis.

Involvement of Cot/Tp12 in Bone Loss during Periodontitis

2010 ◽  
Vol 89 (2) ◽  
pp. 192-197 ◽  
Author(s):  
T. Ohnishi ◽  
A. Okamoto ◽  
K. Kakimoto ◽  
K. Bandow ◽  
N. Chiba ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Periodontal Tissue ◽  
Rankl Expression ◽  
Periodontal Tissues ◽  
Tnf Α ◽  
Experimental Periodontitis ◽  
Deficient Mice

Periodontitis causes resorption of alveolar bone, in which RANKL induces osteoclastogenesis. The binding of lipopolysaccharide to Toll-like receptors causes phosphorylation of Cot/Tp12 to activate the MAPK cascade. Previous in vitro studies showed that Cot/Tp12 was essential for the induction of RANKL expression by lipopolysaccharide. In this study, we examined whether Cot/Tp12 deficiency reduced the progression of alveolar bone loss and osteoclastogenesis during experimental periodontitis. We found that the extent of alveolar bone loss and osteoclastogenesis induced by ligature-induced periodontitis was decreased in Cot/Tp12-deficient mice. In addition, reduction of RANKL expression was observed in periodontal tissues of Cot/Tp12-deficient mice with experimental periodontitis. Furthermore, we found that Cot/Tp12 was involved in the induction of TNF-α mRNA expression in gingiva of mice with experimental periodontitis. Our observations suggested that Cot/Tp12 is essential for the progression of alveolar bone loss and osteoclastogenesis in periodontal tissue during experimental periodontitis mediated through increased RANKL expression.

scholarly journals Protective Effect of Resveratrol against the Alveolar Bone Loss in Rats with Experimental Periodontitis and Acts Positively on the IL-17

2021 ◽  
pp. 162-173
Author(s):  
JordanaHeidemann Pandini ◽  
Lais Fernanda Pasqualotto ◽  
Pedro Henrique de Carli Rodrigues ◽  
João Paulo Gonçalves De Paivaa ◽  
Patricia Oehlmeyer Nassar ◽  
...  
Keyword(s):  
Bone Loss ◽  
Protective Effect ◽  
Alveolar Bone ◽  
Experimental Period ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Interleukin 17 ◽  
Periodontal Tissues ◽  
Male Wistar Rats ◽  
Experimental Periodontitis

The resveratrol is a polyphenol known for its health benefits, which includes the ability to interfere in the osteoblastogenesis, which may foster adverse immunomodulators effects in the host response to periodontal disease. In the present study we evaluated the appearance of periodontal tissues of rats with experimentally induced periodontitis, by using resveratrol. Twenty-four male Wistar rats were used, in which half of the animals received a ligature around the first lower molars, then forming the groups with experimental periodontitis. Next, four groups were created: 1) Control Group (CON); 2) The Ligature Group (LIG); 3) Group Resveratrol (RSV); 4) Ligature-Resveratrol Group (LIG-RSV). The animals of the Resveratrol groups were daily dosed with 10 mg/kg of body weight of polyphenol orally, during four weeks. After 105 days of experimental period, euthanasia was performed. The results showed a significantly lower alveolar bone loss (p<0.05) in animals that received resveratrol, and still, the polyphenol was able to reduce concentration of interleukin 17 (IL-17) in the groups dosed with it. Our conclusion is that dosing rats with experimental periodontitis with resveratrol could cause a protective effect on the alveolar bone loss, in addition to act positively on the IL-17.

scholarly journals Association between Postmenopausal Osteoporosis and Experimental Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kai Luo ◽  
Souzhi Ma ◽  
Jianbin Guo ◽  
Yongling Huang ◽  
Fuhua Yan ◽  
...  
Keyword(s):  
Postmenopausal Osteoporosis ◽  
Alveolar Bone ◽  
Ovariectomized Rats ◽  
Whole Body ◽  
Inflammatory Factors ◽  
Mineral Density ◽  
Metabolic Markers ◽  
Periodontal Tissues ◽  
Experimental Periodontitis ◽  
Turnover Markers

To investigate the correlation between postmenopausal osteoporosis (PMO) and the pathogenesis of periodontitis, ovariectomized rats were generated and the experimental periodontitis was induced using a silk ligature. The inflammatory factors and bone metabolic markers were measured in the serum and periodontal tissues of ovariectomized rats using an automatic chemistry analyzer, enzyme-linked immunosorbent assays, and immunohistochemistry. The bone mineral density of whole body, pelvis, and spine was analyzed using dual-energy X-ray absorptiometry and image analysis. All data were analyzed using SPSS 13.0 statistical software. It was found that ovariectomy could upregulate the expression of interleukin- (IL-)6, the receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG) and downregulate IL-10 expression in periodontal tissues, which resulted in progressive alveolar bone loss in experimental periodontitis. This study indicates that changes of cytokines and bone turnover markers in the periodontal tissues of ovariectomized rats contribute to the damage of periodontal tissues.

scholarly journals Стан системи нітроген (ІІ) оксиду в щурів з пародонтитом на фоні гіпер- та гіпотиреозу

2018 ◽  
Author(s):  
V. V. Shcherba ◽  
M. M. Korda
Keyword(s):  
Periodontal Disease ◽  
No Synthase ◽  
The Body ◽  
Male Rats ◽  
Tissue Homogenate ◽  
Control Group ◽  
Periodontal Tissue ◽  
Social Significance ◽  
Periodontal Tissues ◽  
Experimental Periodontitis

Introduction. Inflammatory periodontal disease is one of the most urgent problems of dentistry, which has a social significance due to the high prevalence, pronounced changes in the tissues of the periodontal disease and the body of the patient as a whole, and the defeat of young people.The aim of the study – to investigate the functional state of the nitrogen (II) oxide system in rats with periodontitis without concomitant pathology and against the background of hyper- and hypothyroidism.Research Methods. The study was carried out on 48 white non-linear male rats. The total activity of NO-synthase (NOS) was determined colorimetrically by the number of formed nitrates and nitrites in the incubation medium. The total content of nitrates and nitrites (NOx) was determined by the Gris method.Results and Discussion. Experimental periodontitis is accompanied by increased general activity of NO-synthase in periodontal tissue homogenate by 2.2 times vs control. NOx content in the serum of animals with periodontitis increased by 46.2% and in the periodontal tissue homogenate – by 74.7% compared with the control. In rats with periodontitis against hyperthyroidism, NOS activity increased by 3.9 times relative to the control group of animals and by 75.9% exceeded the rate of rats with periodontitis without concomitant pathology. In rats with periodontitis, against the background of hypothyroidism, the activity of NOS was 29.6% higher than that of rats with periodontitis without concomitant pathology and 2.9-fold of control.Conclusions. Experimental periodontitis is accompanied by a marked increase in the intensity of nitroxidergic processes both in the homogenate of periodontal tissues and in the blood. The imbalance of thyroid hormones increases the synthesis of nitrogen (II) oxide in the experimental periodontitis, especially expressed in hyperthyroidism.

scholarly journals Evaluation of the Effect of Melatonin on Periodontal Tissues in Rats with Periodontitis Induced Experimentally

2020 ◽  
pp. 115-126
Author(s):  
Bianca Caroline Custodio dos Santos ◽  
Jossinelma Camargo Gomes ◽  
Angela Esmeralda Zaparolli Miola ◽  
Simone Karine Rothen ◽  
Célia Patricia Muller Rodrigues ◽  
...  
Keyword(s):  
Bone Loss ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Bone Cell ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Periodontal Tissues ◽  
Melatonin Administration ◽  
Male Wistar Rats ◽  
Experimental Periodontitis

Objective: To analyze the effects of melatonin administration on the periodontal tissues of rats, linked or not with ligature-induced periodontal disease. Materials and Methods: 40 male Wistar rats aged eight weeks, divided into Control Group (GCON), Ligature Group (GLIG), Melatonin Group (GMEL) and Ligature and Melatonin Group (GLIGMEL). GLIG and GLIGMEL were induced to experimental periodontitis by placing a ligature on the lower molars for 30 days. During the experiment, after 16 days with the ligature, melatonin was administered orally for 10 mg/kg for 14 days in GMEL and GLIGMEL. In the end, euthanasia was performed and the hemi-mandibles were collected for the respective histological and radiographic analyzes; for the results, Shapiro-Wilk, ANOVA-One-Way and Tukey's multiple comparison tests were used. Results: A significantly lower alveolar bone loss (p<0.05) was demonstrated in the animals that received the administration of melatonin, in which it had a prophylactic function against the effects of the disease, evidenced in radiographic, histomorphometric and histological analyzes in the bone cell count. Conclusion: Results show that the therapy with administration of melatonin promotes a protective effect on the alveolar bone tissue of rats with induced experimental periodontitis.

The Effect of Whole Bee Venom on Arthritis

2002 ◽  
Vol 30 (01) ◽  
pp. 73-80 ◽  
Author(s):  
Seong Soo Kang ◽  
Sok Cheon Pak ◽  
Seok Hwa Choi
Keyword(s):  
Physiological Saline ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Honeybee Venom ◽  
Treatment Groups ◽  
Cell Counts ◽  
Physiological Saline Solution ◽  
White Blood Cell Counts ◽  
The Right

This study was performed to assess the clincotherapeutic effect of whole venom of honeybee (Apis mellifera) in adjuvant-induced arthritic rat. Ninety Sprague-Dawley male rats were injected with complete Freund's adjuvant (CFA). Adjuvant arthritis was produced by a single subcutaneous injection of 1 mg Mycobacterium butyricum suspended in 0.1 ml paraffin oil into the right hind paw. Righting reflex was uniformly lost and considered to be the point of arthritis development on day 14 after CFA injection. The experiments were divided into three groups. When arthritis was developed in the rat, tested groups were administered with prednisolone (10 mg/kg, p.o.) or honeybee venom (one bee, s.c.) every other day for another 14 days. Control group was injected with 0.1 ml of physiological saline solution subcutaneously. Clinical and hematological values with histopathological findings were observed during the drug administration. In treatment groups, the development of inflammatory edema and polyarthritis was suppressed. No significant differences of hind paw edema volume and lameness score between prednisolone and honeybee venom groups were observed during treatment. White blood cell counts of control group showed leucocytosis that was significantly different from the two treatment groups (p < 0.01). Erosions of articular cartilage and inflammatory cell infiltrations into interphalangeal joint were effectively suppressed in treated groups. In conclusion, whole honeybee venom was found to suppress arthritic inflammation in the rat. This may be an alternative treatment of arthritic agony in humans.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

Sign in / Sign up

Export Citation Format

Share Document