scholarly journals Sampling Frequency Is a Key Factor to the Efficient Actuation of the Phytohormones in the Increase of Biomass and Macromolecules Production by Spirulina (Arthorspira)

2021 ◽  
Author(s):  
Jéssica Teixeira Silveira ◽  
Ana Priscila Centeno Rosa ◽  
Jorge Alberto Vieira Costa
Keyword(s):  
Large Scale ◽  
Production Capacity ◽  
Biomass Accumulation ◽  
Sampling Frequency ◽  
Cultivation System ◽  
New Strategy ◽  
Key Factor ◽  
Daily Sampling ◽  
Cultivation Strategy ◽  
Operational Aspects

Abstract Application of microalgae in industries is limited by their low biomass production and low macromolecule content. Phytohormones are being studied to solve these problems, however is necessary to define conditions that enhance the results for this kind of cultivation strategy. The increasing of the contact time between substances and cells can be a factor of the improvement in the phytohormone uptake by microalgae. We hypothesize that by decreasing the sampling frequency for monitoring culture parameters, we would also be interfering less in the uptake of phytohormones by microalgae. Therefore, the present study aimed to investigate the influence of sampling frequency on the production of biomass and biomolecules of Spirulina sp. LEB 18 in cultivations supplemented with trans-zeatin and indole-3-acetic acid (IAA). Treatments with lower sampling frequency and supplementation of 1 mg L− 1 IAA enhanced biomass accumulation by 75 %, carbohydrate content by 50 %, and protein content by 30 % as compared to control experiment daily sampling. In addition, sampling every 10 days with 0,01 mg L− 1 IAA supplementation increased the lipid content by 51 % as compared with the experiment without phytohormone and daily sample removal. Therefore, we developed a new strategy to supplement phytohormones and improve microalgal production. This investigation presented a cultivation system efficient, which can be easy to implement in industries because of no need to change the operational aspects of the cultivation. Furthermore, the strategy will can be very useful to enhance the production capacity of microalgae on a large-scale.

Going the Distance

2020 ◽  
Author(s):  
Ron Harris
Keyword(s):  
Large Scale ◽  
Western Europe ◽  
Family Firms ◽  
Global Trade ◽  
Long Distance ◽  
Business Corporation ◽  
Modern Economy ◽  
Key Factor ◽  
Silk Route ◽  
Passive Investors

Before the seventeenth century, trade across Eurasia was mostly conducted in short segments along the Silk Route and Indian Ocean. Business was organized in family firms, merchant networks, and state-owned enterprises, and dominated by Chinese, Indian, and Arabic traders. However, around 1600 the first two joint-stock corporations, the English and Dutch East India Companies, were established. This book tells the story of overland and maritime trade without Europeans, of European Cape Route trade without corporations, and of how new, large-scale, and impersonal organizations arose in Europe to control long-distance trade for more than three centuries. It shows that by 1700, the scene and methods for global trade had dramatically changed: Dutch and English merchants shepherded goods directly from China and India to northwestern Europe. To understand this transformation, the book compares the organizational forms used in four major regions: China, India, the Middle East, and Western Europe. The English and Dutch were the last to leap into Eurasian trade, and they innovated in order to compete. They raised capital from passive investors through impersonal stock markets and their joint-stock corporations deployed more capital, ships, and agents to deliver goods from their origins to consumers. The book explores the history behind a cornerstone of the modern economy, and how this organizational revolution contributed to the formation of global trade and the creation of the business corporation as a key factor in Europe's economic rise.

scholarly journals Short loop functional commonality identified in leukaemia proteome highlights crucial protein sub-networks

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Sun Sook Chung ◽  
Joseph C F Ng ◽  
Anna Laddach ◽  
N Shaun B Thomas ◽  
Franca Fraternali
Keyword(s):  
Protein Interactions ◽  
Large Scale ◽  
Interaction Network ◽  
Protein Protein Interactions ◽  
Protein Protein Interaction ◽  
Ppi Networks ◽  
Short Loop ◽  
New Strategy ◽  
Loop Network ◽  
Protein Protein Interaction Network

Abstract Direct drug targeting of mutated proteins in cancer is not always possible and efficacy can be nullified by compensating protein–protein interactions (PPIs). Here, we establish an in silico pipeline to identify specific PPI sub-networks containing mutated proteins as potential targets, which we apply to mutation data of four different leukaemias. Our method is based on extracting cyclic interactions of a small number of proteins topologically and functionally linked in the Protein–Protein Interaction Network (PPIN), which we call short loop network motifs (SLM). We uncover a new property of PPINs named ‘short loop commonality’ to measure indirect PPIs occurring via common SLM interactions. This detects ‘modules’ of PPI networks enriched with annotated biological functions of proteins containing mutation hotspots, exemplified by FLT3 and other receptor tyrosine kinase proteins. We further identify functional dependency or mutual exclusivity of short loop commonality pairs in large-scale cellular CRISPR–Cas9 knockout screening data. Our pipeline provides a new strategy for identifying new therapeutic targets for drug discovery.

scholarly journals Glucose-Limited Fed-Batch Cultivation Strategy to Mimic Large-Scale Effects in Escherichia coli Linked to Accumulation of Non-Canonical Branched-Chain Amino Acids by Combination of Pyruvate Pulses and Dissolved Oxygen Limitation

2021 ◽  
Vol 9 (6) ◽  
pp. 1110
Author(s):  
Ángel Córcoles García ◽  
Peter Hauptmann ◽  
Peter Neubauer
Keyword(s):  
Amino Acids ◽  
Dissolved Oxygen ◽  
Large Scale ◽  
Scale Effects ◽  
Branched Chain Amino Acids ◽  
Oxygen Limitation ◽  
Fed Batch ◽  
Fermentation Products ◽  
Cultivation Strategy ◽  
Branched Chain

Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low oxygen feeding zone with the accumulation of mixed acid fermentation products, especially formate, but also with the synthesis of non-canonical amino acids, such as norvaline, norleucine and β-methylnorleucine. These amino acids can be mis-incorporated into recombinant products, which causes a problem for pharmaceutical production whose solution is not trivial. While these effects can also be observed in scale down bioreactor systems, these are challenging to operate. Especially the high-throughput screening of clone libraries is not easy, as fed-batch cultivations would need to be controlled via repeated glucose pulses with simultaneous oxygen limitation, as has been demonstrated in well controlled robotic systems. Here we show that not only glucose pulses in combination with oxygen limitation can provoke the synthesis of these non-canonical branched-chain amino acids (ncBCAA), but also that pyruvate pulses produce the same effect. Therefore, we combined the enzyme-based glucose delivery method Enbase® in a PALL24 mini-bioreactor system and combined repeated pyruvate pulses with simultaneous reduction of the aeration rate. These cultivation conditions produced an increase in the non-canonical branched chain amino acids norvaline and norleucine in both the intracellular soluble protein and inclusion body fractions with mini-proinsulin as an example product, and this effect was verified in a 15 L stirred tank bioreactor (STR). To our opinion this cultivation strategy is easy to apply for the screening of strain libraries under standard laboratory conditions if no complex robotic and well controlled parallel cultivation devices are available.

scholarly journals Trace element catalyses mineral replacement reactions and facilitates ore formation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yanlu Xing ◽  
Joël Brugger ◽  
Barbara Etschmann ◽  
Andrew G. Tomkins ◽  
Andrew J. Frierdich ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Trace Elements ◽  
Fluid Flow ◽  
Large Scale ◽  
Metal Accumulation ◽  
Nucleation And Growth ◽  
Reaction Interface ◽  
Ore Formation ◽  
Key Factor ◽  
Mineral Replacement

AbstractReaction-induced porosity is a key factor enabling protracted fluid-rock interactions in the Earth’s crust, promoting large-scale mineralogical changes during diagenesis, metamorphism, and ore formation. Here, we show experimentally that the presence of trace amounts of dissolved cerium increases the porosity of hematite (Fe2O3) formed via fluid-induced, redox-independent replacement of magnetite (Fe3O4), thereby increasing the efficiency of coupled magnetite replacement, fluid flow, and element mass transfer. Cerium acts as a catalyst affecting the nucleation and growth of hematite by modifying the Fe2+(aq)/Fe3+(aq) ratio at the reaction interface. Our results demonstrate that trace elements can enhance fluid-mediated mineral replacement reactions, ultimately controlling the kinetics, texture, and composition of fluid-mineral systems. Applied to some of the world’s most valuable orebodies, these results provide new insights into how early formation of extensive magnetite alteration may have preconditioned these ore systems for later enhanced metal accumulation, contributing to their sizes and metal endowment.

Decrease in proven ventriculitis by reducing the frequency of cerebrospinal fluid sampling from extraventricular drains

2011 ◽  
Vol 115 (5) ◽  
pp. 1040-1046 ◽  
Author(s):  
Teresa A. Williams ◽  
Gavin D. Leslie ◽  
Geoffrey J. Dobb ◽  
Brigit Roberts ◽  
Peter Vernon van Heerden
Keyword(s):  
Head Injuries ◽  
Tertiary Hospital ◽  
Sampling Frequency ◽  
Apache Ii Score ◽  
Optimum Frequency ◽  
Clinical Indicators ◽  
Historical Comparison ◽  
Institutional Ethics ◽  
Daily Sampling ◽  
Definition Of

Object Ventriculitis associated with extraventricular drains (EVD) increases rates of morbidity and mortality as well as costs. Surveillance samples of CSF are taken routinely from EVD, but there is no consensus on the optimum frequency of sampling. The goal of this study was to assess whether the incidence of ventriculitis changed when CSF sampling frequency was reduced once every 3 days. Methods After receiving institutional ethics committee approval for their project, the authors compared a prospective sample of EVD-treated patients (admitted 2008–2009) and a historical comparison group (admitted 2005–2007) at two tertiary hospital ICUs. A broad definition of ventriculitis included suspected ventriculitis (that is, treated with antibiotics for ventriculitis) and proven ventriculitis (positive CSF culture). Adult ICU patients with no preexisting neurological infection were enrolled in the study. After staff was provided with an education package, sampling of CSF was changed from daily to once every 3 days. All other management of the EVD remained unchanged. More frequent sampling was permitted if clinically indicated during the third daily sampling phase. Results Two hundred seven patients were recruited during the daily sampling phase and 176 patients when sampling was reduced to once every 3 days. The Acute Physiology and Chronic Health Evaluation (APACHE) II score was lower for the daily sampling group than for the every-3rd-day group (18.6 vs 20.3, respectively; p < 0.01), but there was no difference in mean age (47 and 45 years, respectively; p = 0.14), male or female sex (61% and 59%, respectively; p = 0.68), or median EVD duration in the ICU (4.9 and 5.8 days, respectively; p = 0.14). Most patients were admitted with subarachnoid hemorrhage (42% in the daily group and 33% in the every-3rd-day group) or traumatic head injuries (29% and 36%, respectively). The incidence of ventriculitis decreased from 17% to 11% overall and for proven ventriculitis from 10% to 3% once sampling frequency was reduced. Sampling of CSF once every 3 days was independently associated with ventriculitis (OR 0.44, 95% CI 0.22–0.88, p = 0.02). Conclusions Reducing the frequency of CSF sampling to once every 3 days was associated with a significant decrease in the incidence of ventriculitis. The authors suggest that CSF sampling should therefore be performed once every 3 days in the absence of clinical indicators of ventriculitis. Reducing frequency of CSF sampling from EVDs decreased proven ventriculitis.

What determines a boundary for navigating a complex street network: evidence from London taxi drivers

2021 ◽  
pp. 1-20
Author(s):  
Eva-Maria Griesbauer ◽  
Ed Manley ◽  
Daniel McNamee ◽  
Jeremy Morley ◽  
Hugo Spiers
Keyword(s):  
Urban Space ◽  
Large Scale ◽  
Route Planning ◽  
Taxi Drivers ◽  
Street Networks ◽  
Planning Models ◽  
Key Factor ◽  
Efficient Planning ◽  
Regent's Park ◽  
Insight Into

Abstract Spatial boundaries play an important role in defining spaces, structuring memory and supporting planning during navigation. Recent models of hierarchical route planning use boundaries to plan efficiently first across regions and then within regions. However, it remains unclear which structures (e.g. parks, rivers, major streets, etc.) will form salient boundaries in real-world cities. This study tested licensed London taxi drivers, who are unique in their ability to navigate London flexibly without physical navigation aids. They were asked to indicate streets they considered as boundaries for London districts or dividing areas. It was found that agreement on boundary streets varied considerably, from some boundaries providing almost no consensus to some boundaries consistently noted as boundaries. Examining the properties of the streets revealed that a key factor in the consistent boundaries was the near rectilinear nature of the designated region (e.g. Mayfair and Soho) and the distinctiveness of parks (e.g. Regent's Park). Surprisingly, the River Thames was not consistently considered as a boundary. These findings provide insight into types of environmental features that lead to the perception of explicit boundaries in large-scale urban space. Because route planning models assume that boundaries are used to segregate the space for efficient planning, these results help make predictions of the likely planning demands of different routes in such complex large-scale street networks. Such predictions could be used to highlight information used for navigation guidance applications to enable more efficient hierarchical planning and learning of large-scale environments.

scholarly journals Synthesis of the Sparse Uniform-Amplitude Concentric Ring Transmitting Array for Optimal Microwave Power Transmission

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Hua-Wei Zhou ◽  
Xue-Xia Yang ◽  
Sajjad Rahim
Keyword(s):  
Microwave Power ◽  
Large Scale ◽  
Power Transmission ◽  
Synthesis Method ◽  
Pso Algorithm ◽  
Practical Significance ◽  
Concentric Ring ◽  
Swarm Optimization ◽  
Key Factor ◽  
Feed Network

Beam capture efficiency (BCE) is one key factor of the overall efficiency for a microwave power transmission (MPT) system, while sparsification of a large-scale transmitting array has a practical significance. If all elements of the transmitting array are excited uniformly, the fabrication, maintenance, and feed network design would be greatly simplified. This paper describes the synthesis method of the sparse uniform-amplitude transmitting array with concentric ring layout using particle swarm optimization (PSO) algorithm while keeping a higher BCE. Based on this method, uniform exciting strategy, reduced number of elements, and a higher BCE are achieved simultaneously for optimal MPT. The numerical results of the sparse uniform-amplitude concentric ring arrays (SUACRAs) optimized by the proposed method are compared with those of the random-located uniform-amplitude array (RLUAA) and the stepped-amplitude array (SAA), both being reported in the literatures for the maximum BCE. Compared to the RLUAA, the SUACRA saves 32% elements with a 1.1% higher BCE. While compared to the SAA, the SUACRA saves 29.1% elements with a bit higher BCE. The proposed SUACRAs have higher BCEs, simple array arrangement and feed network, and could be used as the transmitting array for a large-scale MPT system.

Optimal Operation and Maintenance of Gas Compressor Stations: An Integrated Framework Applied to a Large-Scale Industrial Case

2015 ◽  
Vol 138 (4) ◽  
Author(s):  
Dionysios P. Xenos ◽  
Erling Lunde ◽  
Nina F. Thornhill
Keyword(s):  
Optimization Model ◽  
Large Scale ◽  
Optimal Schedule ◽  
Optimal Operation ◽  
Optimal Distribution ◽  
Compressor Station ◽  
Operational Costs ◽  
Operation And Maintenance ◽  
Maintenance Model ◽  
Operational Aspects

This paper presents a framework which integrates maintenance and optimal operation of multiple compressors. The outcome of this framework is a multiperiod plan which provides the schedule of the operation of compressors: the schedule gives the best decisions to be taken, for example, when to carry out maintenance, which compressors to use online and how much to load them. These decisions result in the minimization of the total operational costs of the compressors while at the same time the demand of the plant is met. The suggested framework is applied to an industrial gas compressor station which encompasses large multistage centrifugal compressors operating in parallel. The optimization model of the framework consists of three main parts: the models of compressor maps, the operational aspects of compressors, and a maintenance model. The results illustrate the optimal schedule for 90 days and an example of the optimal distribution of the load of the compressors for 5 days. Finally, the results show the economical benefits from the integration of maintenance and optimization.

Performance and Benefit Analysis of Mining Accumulator Locomotive AC Speed System Based on DTC

2011 ◽  
Vol 328-330 ◽  
pp. 1846-1850
Author(s):  
Xi Zhang ◽  
Jie Yang ◽  
Wen Chao Chen ◽  
Qi Zhou Huang
Keyword(s):  
Large Scale ◽  
Asynchronous Motor ◽  
Speed Control ◽  
Fast Response ◽  
Efficient Operation ◽  
Ac Drive ◽  
Key Factor ◽  
Large Scale Integration ◽  
Overload Capacity ◽  
Scale Integration

The traditional mine accumulator locomotive usually takes the DC motor for its power source. And the series-wound resistance method is usually used in its speed control. With the development of power electronics technology, the AC drive system based on power electronic converters can be realized. Especially after the realization of large-scale integration and compmer controling, the performance of AC variale speed has been improved a lot. In operation, the mine accumulator locomotive not only works in frequent starting, breaking, accelerating and decelerating conditions, but also has to adapt to the bad pavement. The AC asynchronous motor has simple stmcture, sturdy and durable, and it could save power by notusing resistance when it was used in speed control. With this characteristics, the AC speed control system has the feature of fast response and high overload capacity. The AC speed system is the key factor for efficient operation of mining accumulator locomotive. Compared with the traditional DC speed system, the principle of DTC is introduced in this paper. And the advantage of operating performance and economic benefit of mining accumulator locomotive with AC speed system based on DTC is analyzed from practical application.

Effects of Weld Strength Heterogeneity on Crack Driving Force in Stress and Strain Based Design Scenarios

2014 ◽  
Author(s):  
Stijn Hertelé ◽  
Noel O’Dowd ◽  
Matthias Verstraete ◽  
Koen Van Minnebruggen ◽  
Wim De Waele
Keyword(s):  
Driving Force ◽  
Large Scale ◽  
Limit State ◽  
Weld Strength ◽  
Force Response ◽  
Crack Driving Force ◽  
Key Factor ◽  
Weld Properties ◽  
Girth Welds ◽  
Strain Hardening Behavior

Weld strength mismatch is a key factor with respect to the assessment of a flawed girth weld. However, it is challenging to assign a single strength mismatch value to girth welds, which are generally heterogeneous in terms of constitutive behavior. The authors have recently developed a method (‘homogenization’) to account for weld strength property variations in the estimation of crack driving force response and the corresponding tensile limit state. This paper separately validates the approach for stress based and strain based assessments. Whereas homogenization is reliably applicable for stress based assessments, the strain based crack driving force response is highly sensitive to effects of actual heterogeneous weld properties. The sensitivity increases with increased weld width and decreased strain hardening behavior. For strain based design, a more accurate methodology is desirable, and large scale testing and/or advanced numerical modeling remain essential.

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