Ophiostomatoid Fungi Associated with Cryphalus Piceae in Shandong province in eastern China

2021 ◽  
Author(s):  
Runlei Chang ◽  
Xiuyu Zhang ◽  
Hongli Si ◽  
Guoyan Zhao ◽  
Xiaowen Yuan ◽  
...  
Keyword(s):  
Bark Beetles ◽  
Novel Species ◽  
Eastern China ◽  
Fungal Species ◽  
Shandong Province ◽  
Morphological Data ◽  
Ophiostomatoid Fungi ◽  
Fungal Isolates ◽  
Study Sites ◽  
Isolated Species

Abstract Cryphalus piceae parasitizes various economically important conifers. Similar to other bark beetles, C. picea vectors an assortment of fungi and nematodes. Previously, several ophiostomatoid fungi were isolated from C. piceae in Poland and Japan. In the present study, we explored the diversity of ophiostomatoid fungi associated with C. piceae infesting pines in the Shandong Province of China. We isolated ophiostomatoid fungi from both galleries and beetles collected from our study sites. These fungal isolates were identified using both molecular and morphological data. Through this study, we recovered 176 isolates of ophiostomatoid fungi representing at least seven species. Ophiostoma ips was the most frequently isolated species. Analyses of molecular and morphological data indicated four of the ophiostomatoid fungal species recovered in this study were previously undescribed. Hereby, we described these species as Ceratocystiopsis yantaiensis sp. nov., C. weihaiensis sp. nov., Graphilbum translucens sp. nov. and Sporothrix villosa sp. nov. A majority of the ophiostomatoid fungi recovered in this study were novel species. This suggests that the forests in China harbour an assortment of undescribed ophiostomatoid fungi yet to be discovered.

scholarly journals Ophiostomatoid species associated with pine trees (Pinus spp.) infested by Cryphalus piceae from eastern China, including five new species

MycoKeys ◽  
2021 ◽  
Vol 83 ◽  
pp. 181-208
Author(s):  
Runlei Chang ◽  
Xiuyu Zhang ◽  
Hongli Si ◽  
Guoyan Zhao ◽  
Xiaowen Yuan ◽  
...  
Keyword(s):  
Bark Beetles ◽  
Eastern China ◽  
Fungal Species ◽  
Morphological Data ◽  
Ophiostomatoid Fungi ◽  
Pine Trees ◽  
Novel Taxa ◽  
Study Sites ◽  
Pinus Spp ◽  
Isolated Species

Cryphalus piceae attacks various economically important conifers. Similar to other bark beetles, Cr. piceae plays a role as a vector for an assortment of fungi and nematodes. Previously, several ophiostomatoid fungi were isolated from Cr. piceae in Poland and Japan. In the present study, we explored the diversity of ophiostomatoid fungi associated with Cr. piceae infesting pines in the Shandong Province of China. We isolated ophiostomatoid fungi from both galleries and beetles collected from our study sites. These fungal isolates were identified using both molecular and morphological data. In this study, we recovered 175 isolates of ophiostomatoid fungi representing seven species. Ophiostoma ips was the most frequently isolated species. Molecular and morphological data indicated that five ophiostomatoid fungal species recovered were previously undescribed. Thus, we proposed these five novel species as Ceratocystiopsis yantaiensis, C. weihaiensis, Graphilbum translucens, Gr. niveum, and Sporothrix villosa. These new ophiostomatoid fungi add to the increasing number of fungi known from China, and this evidence suggests that numerous novel taxa are awaiting discovery in other forests of China.

scholarly journals Mycobiota associated with Hylurgops palliatus (Gyll.) on Pinus sylvestris L. in Poland

2011 ◽  
Vol 75 (4) ◽  
pp. 333-338 ◽  
Author(s):  
Robert Jankowiak
Keyword(s):  
Pinus Sylvestris ◽  
Dominant Species ◽  
Fungal Species ◽  
Ophiostomatoid Fungi ◽  
Important Group ◽  
Fungal Isolates ◽  
Unknown Species ◽  
Hylurgops Palliatus

The assemblage and abundance of fungal species associated with <em>Hylurgops palliatus</em> on <em>Pinus sylvestris</em>, were studied in Poland. Fungi were isolated from beetles of <em>H. palliatus</em> and their galleries collected from three populations. In total, 1832 fungal isolates, including 38 species, were obtained. The most important group of fungi were the ophiostomatoid fungi. Among the seven species of ophiostomatoid fungi isolated, <em>Leptographium lundbergii</em> and an unknown species of <em>Graphium</em> sp. code-named "W" were the dominant species. <em>Ophiostoma minus</em> and <em>O. piceae</em> were also relatively common. All ophiostomatoid species, except <em>L. lundbergii</em> occurred more frequently on the overwintered adults than in gallery systems. <em>Ophiostoma minus</em> and <em>O. piliferum</em> are documented here as new associates of <em>H. palliatus</em>.

scholarly journals Fungal communities associated with symptomatic leaves of naranjilla (Solanum quitoense Lam.) in the Ecuadorian Amazon region

2018 ◽  
Vol 39 (1) ◽  
Author(s):  
María Fernanda Dávila ◽  
Wagner Chaves-Acuña ◽  
Jeniffer Yánez
Keyword(s):  
Biological Control ◽  
Fungal Species ◽  
Control Measures ◽  
Fungal Communities ◽  
Production Region ◽  
Fungal Isolates ◽  
Ecuadorian Amazon ◽  
Pharmaceutical Properties ◽  
First Time ◽  
Isolated Species

Fungal isolates were recovered from symptomatic leaves of naranjilla Solanum quitoense Lam. sampled in Pastaza province, an important production region in eastern Ecuadorian Amazon. Filamentous fungi were identified with molecular methods based on ITS and ribosomal DNA sequencing. Among 68 recorded fungi, the isolation of mycobiota resulted in 14 taxa. Diaporthe citri, D. schini, and Xylaria berteri were the most frequently isolated species. To our knowledge more than 90 % of taxa recovered in this study are reported for the first time in S. quitoense. Diversity indexes suggest that symptomatic tissues represent a moderately diverse reservoir for fungal species. The latter may act as a significant source of novel molecules with agronomic and pharmaceutical properties considering their capacity to survive within unhealthy foliar tissues. We also expect this study to facilitate the enforcement of biological control measures in Neotropical crops of commercial interest.

Diversity of ophiostomatoid fungi associated with bark beetles (Coleoptera: Scolytidae) colonizing branches of Norway spruce (Picea abies) in southern Poland

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Robert Jankowiak ◽  
Magdalena Kacprzyk ◽  
Marta Młynarczyk
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Bark Beetles ◽  
Fungal Species ◽  
Ips Typographus ◽  
Ophiostomatoid Fungi ◽  
Southern Poland ◽  
Fungal Associates ◽  
Ceratocystis Polonica ◽  
Fungal Associate

AbstractBark beetles (Coleoptera: Scolytidae) infesting Norway spruce trees are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Poland. In this study, we examined the ophiostomatoid species associated with seven species of bark beetles developing in Norway spruce (Picea abies). Fungi were isolated from the beetles and their galleries at ten sites in southern Poland. We identified a total of 2,769 fungal isolates that belong to 19 ophiostomatoid species. These 19 species included a total of 16 associations between fungi and bark beetles that had not been previously recorded. The isolated fungal species were similar to those previously reported. The species spectrum and relative abundance of fungal associates were similar in seven bark beetle species, despite some differences between species. Except for Pityophthorus pityographus, all bark beetles species were frequently associated with ophiostomatoid fungi. The most commonly encountered fungal associates of bark beetles were the following: Ophiostoma ainoae, O. bicolor, O. piceae sensu lato and Grosmannia piceiperda. The results support the hypothesis that pathogenic ophiostomatoid fungi are important for aggressive bark beetles to kill trees. The most virulent fungal associate of bark beetles, Ceratocystis polonica was most commonly associated with Ips typographus. The most frequent ophiostomatoid species isolated from the beetles and galleries of P. pityographus were O. ainoae and Graphium fimbriisporum.

Discovery and Geological Significance of Neoproterozoic Metamorphic Granite in Jimo, Shandong Province, Eastern China

2016 ◽  
Vol 90 (6) ◽  
pp. 2080-2096 ◽  
Author(s):  
Decheng ZHU ◽  
Dawei LÜ ◽  
Xiaoli SHEN ◽  
Qing YANG ◽  
Dandan LI ◽  
...  
Keyword(s):  
Eastern China ◽  
Shandong Province ◽  
Geological Significance

Screening and Identification of Laccase Producing Fungi from Environmental Samples

2021 ◽  
Vol 6 (1) ◽  
pp. 91-98
Author(s):  
A. Bello ◽  
◽  
J. B. Ameh ◽  
D. A. Machido ◽  
A. I. Mohammed-Dabo
Keyword(s):  
Tannic Acid ◽  
Fungal Species ◽  
Soil Samples ◽  
Potato Dextrose Agar ◽  
Laccase Production ◽  
Curvularia Lunata ◽  
Main Campus ◽  
Fungal Isolates ◽  
Screening And Identification ◽  
Decaying Wood

Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and non-phenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from environment samples. Soil and decaying wood samples were collected from different locations within Ahmadu Bello University, Zaria Main campus. Suspensions of the samples (1 g in 10 mL sterile distilled water) were serially diluted, inoculated onto Potato Dextrose Agar (PDA) containing 0.01% Chloramphenicol and incubated for 7 days at 30oC.The fungal isolates were characterized macroscopically and microscopically with the aid of an atlas. The identified fungal isolates were screened for laccase production by inoculating onto PDA containing 0.02% Guaiacol, 1mM ABTS (2 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 0.5% Tannic acid as indicator compounds and incubated at 250C for 7 days. The laccase producing isolates were confirmed molecularly by ITS rDNA sequence analysis using the FASTA algorithm with the Fungus database from the European Bioinformatics Institute (EBI).A total of 25 fungal species (11 from soil and 14 from decaying wood samples) were isolated. Two isolates from the soil origin identified as Curvularia lunata SSI7 (Accession No. QIE06317.1) and Fusarium clade VII SSI3 (Accession No. GQ505677) were found to produce laccase where Curvularia lunata SSI7 was able to oxidize all the indicator compounds used for the screening. Fusarium clade VII SSI3 was able to oxidize only 0.5% Tannic acid. Laccase producing Curvularia lunata and Fusarium clade VII were isolated from soil samples collected from ABU Zaria Main Campus. Keywords: laccase, fungi, soil, decaying wood

scholarly journals A comparative characteristic of fungal communities associated with Ips acuminatus in different regions of Ukraine

2019 ◽  
pp. 118-128
Author(s):  
Kateryna Davydenko
Keyword(s):  
Bark Beetle ◽  
Fungal Community ◽  
Bark Beetles ◽  
Diversity Index ◽  
Pathogenic Fungi ◽  
Pine Forest ◽  
Comparative Characteristic ◽  
Ophiostomatoid Fungi ◽  
Decay Fungi ◽  
Sphaeropsis Sapinea

Pine bark beetles are typically associated with complexes of fungi that could reveal different functional interaction. Thus, previously nonaggressive bark beetle Ips acuminatus is considering now to be among the most serious pests of pine forest in Ukraine and other European countries and vectored fungal community is very important to assess total harm of this bark beetle. The aim of this study was to reveal the vectored fungal community associated with the pine engraver beetle, I. acuminatus with special emphasis on pathogenic fungi for further evaluation of harm bark-beetle - fungi association for Ukrainian forest. In total, 288 adult beetles were collected from Scots pine trees at six different sites through Ukraine. DNA sequencing as fungal culturing from all beetles resulted in 1681 isolates and amplicons representing 42 fungal taxa. NCBI BLAST search revealed that the overall fungal community was composed of 94 species, of which 80.85% were Ascomycota, followed by Basidiomycota and unidentified fungal group, which accounted for 10.6% and 8.5 % of the total sequences, respectively. Among these, the most commonly detected fungi for pooling dataset were Sphaeropsis sapinea (23.6%), Cladosporium pini-ponderosae (19.44%), Ophiostoma ips (19.1%), Ophiostoma canum (19.1%) and Cladobotryum mycophilum (18.06%). In the pooled dataset of isolates and amplicons for each site, Shannon diversity indices ranged between 1.9 and 2.9 while Simpson diversity index varied between 0.69 and 0.89 indicating rich species diversity. In total twelve ophiostomatoid species were detected. All ophiostomatoid fungi were showing varying degrees of virulence and O. minus was the most aggressive fungus in previous studies. It is concluded that I. acuminatus vectors a species-rich fungal community including pathogens such as ophiostomatoid fungi, Sphaeropsis sapinea, different needle pathogens and wood decay fungi that seems to be very important for the assessment of threat of I. acuminatus to the pine forest in Ukraine.

scholarly journals Seroprevalence of Toxoplasma gondii infection and variables associated with seropositivity in donkeys in eastern China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 66 ◽  
Author(s):  
Qing-Feng Meng ◽  
Dan Li ◽  
Gui-Zhe Yao ◽  
Yang Zou ◽  
Wei Cong ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Feeding Habits ◽  
Eastern China ◽  
Shandong Province ◽  
Eastern Region ◽  
Limited Information ◽  
Equus Asinus ◽  
Toxoplasma Gondii Infection ◽  
Production Areas ◽  
And Control

Donkeys (Equus asinus) are widely distributed throughout China; they are used for their meat, as food, and certain donkey-derived items are also important for traditional Chinese medicinal purposes. However, only limited information is available on Toxoplasma gondii infection in donkeys in China, especially the eastern region, which is one of the largest production areas. Thus, the present study was conducted to detect specific anti-T. gondii antibodies using a commercially available indirect hemagglutination test (IHA) kit and to evaluate the risk factors that are associated with seroprevalence in the Shandong province of eastern China. A total of 213/1278 (17%) donkeys tested from Shandong province were positive for T. gondii antibodies. Statistical analysis revealed that gender and feeding habits of the animal are associated with T. gondii infection. These results provide information for the prevention and control of toxoplasmosis in donkeys, other animals, and humans in this region and elsewhere.

scholarly journals Ophiostomatoid fungi associated with the spruce bark beetle Ips typographus, including 11 new species from China

2019 ◽  
Vol 42 (1) ◽  
pp. 50-74 ◽  
Author(s):  
R. Chang ◽  
T.A. Duong ◽  
S.J. Taerum ◽  
M.J. Wingfield ◽  
X. Zhou ◽  
...  
Keyword(s):  
Bark Beetle ◽  
Dna Sequences ◽  
Eastern China ◽  
Strong Association ◽  
Ips Typographus ◽  
Invasion History ◽  
Ophiostomatoid Fungi ◽  
Population Structures ◽  
Fungal Assemblages ◽  
China And Japan

Ips typographus (Coleoptera, Scolytinae) is a spruce-infesting bark beetle that occurs throughout Europe and Asia. The beetle can cause considerable damage, especially when colonized trees are stressed and beetle populations increase. Although some studies have shown that populations of I. typographus in Europe, China and Japan are genetically distinct, these populations are biologically similar, including a strong association with ophiostomatoid fungi. To date, only two Leptographium spp. have been reported from the beetle in China, while 40 species have been reported from Europe and 13 from Japan. The aims of this study were to identify the ophiostomatoid fungal associates of I. typographus in north-eastern China, and to determine whether the fungal assemblages reflect the different geographical populations of the beetle. Field surveys in Jilin and Heilongjiang provinces yielded a total of 1046 fungal isolates from 145 beetles and 178 galleries. Isolates were grouped based on morphology and representatives of each group were identified using DNA sequences of the ribosomal LSU, ITS, β-tubulin, calmodulin and elongation factor 1-α gene regions. A total of 23 species of ophiostomatoid fungi were identified, including 12 previously described species and 11 novel species, all of which are described here. The dominant species were Ophiostoma bicolor, Leptographium taigense and Grosmannia piceiperda D, representing 40.5 %, 27.8 % and 17.8 % of the isolates, respectively. Comparisons of species from China, Europe and Japan are complicated by the fact that some of the European and all the Japanese species were identified based only on morphology. However, assuming that those identifications are correct, five species were shared between Europe, Japan and China, two species were shared between China and Japan, five between Europe and China, and two between Europe and Japan. Consequently, Ips typographus populations in these different geographic areas have different fungal assemblages, suggesting that the majority of these beetle-associations are promiscuous. The results also suggested that the symbionts of the bark beetle do not reflect the population structures of the beetle. The use of fungal symbiont assemblages to infer population structures and invasion history of its vectors should thus be interpreted with circumspection.

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