Low-Dose Cold Atmospheric Plasma Promoting Schwann Cells Proliferation By Activating PI3K/Akt/mTOR Pathway

Abstract Cold atmospheric plasma (CAP) is an emerging technology that has attracted the attention of many researchers in many fields and disciplines. In this study, a dielectric barrier discharge (DBD) plasma device was used to treat Schwann cells (SCs) cultured in vitro, and the effect of CAP on SCs proliferation was evaluated by cell morphology, cell viability, cell cycle and expression of related proteins in SCs. The results showed that the production of intracellular ROS and RNS increased with the increase of CAP treatment time. Compared with the control group, the proliferation of SCs treated with CAP for less than 14 s significantly increased, and and then gradually decreased. Besides, the cell cycle results also showed that more cells were in the S+G2/M phase at this time.The PI3K/Akt/mTOR pathway was activated by low-dose CAP, and the expression of cyclinD1 was consistent with the trend of cell proliferation. In addition, n-acetyl-L-cysteine (NAC) preconditioning significantly prevented CAP-induced cellular changes. In conclusion, low-dose CAP-induced of SCs proliferation was closely related to the PI3K/Akt/mTOR signaling pathway. This study provides a new idea for the treatment of peripheral nerve injury.

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Contact-Free Inactivation of Candida albicans Biofilms by Cold Atmospheric Air Plasma

Applied and Environmental Microbiology ◽

10.1128/aem.07235-11 ◽

2012 ◽

Vol 78 (12) ◽

pp. 4242-4247 ◽

Cited By ~ 65

Author(s):

Tim Maisch ◽

Tetsuji Shimizu ◽

Georg Isbary ◽

Julia Heinlin ◽

Sigrid Karrer ◽

...

Keyword(s):

Health Care ◽

Candida Albicans ◽

Treatment Time ◽

Atmospheric Plasma ◽

Air Plasma ◽

Content Type ◽

Cold Atmospheric Plasma ◽

Promising Tool ◽

Plasma Device ◽

Contact Free

ABSTRACTCandida albicansis one of the main species able to form a biofilm on almost any surface, causing both skin and superficial mucosal infections. The worldwide increase in antifungal resistance has led to a decrease in the efficacy of standard therapies, prolonging treatment time and increasing health care costs. Therefore, the aim of this work was to demonstrate the applicability of atmospheric plasma at room temperature for inactivatingC. albicansgrowing in biofilms without thermally damaging heat-sensitive materials. This so-called cold atmospheric plasma is produced by applying high voltage to accelerate electrons, which ionize the surrounding air, leading to the production of charged particles, reactive species, and photons. A newly developed plasma device was used, which exhibits a large plasma-generating surface area of 9 by 13 cm (117 cm2). Different time points were selected to achieve an optimum inactivation efficacy range of ≥3 log10to 5 log10reduction in CFU per milliliter, and the results were compared with those of 70% ethanol. The results obtained show that contact-free antifungal inactivation ofCandidabiofilms by cold atmospheric plasma is a promising tool for disinfection of surfaces (and items) in both health care settings and the food industry, where ethanol disinfection should be avoided.

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Development of a portable cold atmospheric plasma device for infected wound management

2017 Medical Technologies National Congress (TIPTEKNO) ◽

10.1109/tiptekno.2017.8238066 ◽

2017 ◽

Author(s):

Yusuf Hakan Usta ◽

Utku Kursat Ercan

Keyword(s):

Atmospheric Plasma ◽

Wound Management ◽

Infected Wound ◽

Cold Atmospheric Plasma ◽

Plasma Device

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Prospective, Comparative Clinical Pilot Study of Cold Atmospheric Plasma Device in the Treatment of Atopic Dermatitis

10.21203/rs.3.rs-453127/v1 ◽

2021 ◽

Author(s):

Young Jae Kim ◽

Dong Jun Lim ◽

Mi Young Lee ◽

Woo Jin Lee ◽

Sung Eun Chang ◽

...

Keyword(s):

Atopic Dermatitis ◽

Pilot Study ◽

Severity Index ◽

Skin Lesions ◽

Treated Group ◽

Atmospheric Plasma ◽

Clinical Severity ◽

Control Treatment ◽

Cold Atmospheric Plasma ◽

Plasma Device

Abstract Introduction: Cold atmospheric plasma generates free radicals through the ionization of air at room temperature. Its effect and safety profile in patients with atopic dermatitis have not been evaluated prospectively.Objective: We aimed to investigate the effect and safety of cold atmospheric plasma in patients with atopic dermatitis with a prospective pilot study.Methods: Cold atmospheric plasma treatment or sham control treatment were applied respectively in randomly assigned and symmetric skin lesions. Three treatment sessions were performed at weeks 0, 1, and 2. Clinical severity indices were assessed at weeks 0, 1, 2, and 4 after treatment. Additionally, the microbial characteristics of the lesions before and after treatments were analyzed.Results: We included 22 patients with mild to moderate atopic dermatitis presented with symmetric lesions. We found that cold atmospheric plasma can alleviate the clinical severity of atopic dermatitis. Modified atopic dermatitis antecubital severity and eczema area and severity index score were significantly decreased in the treated group. Furthermore, scoring of atopic dermatitis score and pruritic visual analog scales significantly improved. In microbiome analysis revealed significantly reduced proportion of Staphylococcus aureus in the treated group.Conclusion: Cold atmospheric plasma can significantly improve mild and moderate atopic dermatitis without safety issues.

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Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet

Journal of Clinical Medicine ◽

10.3390/jcm8111930 ◽

2019 ◽

Vol 8 (11) ◽

pp. 1930 ◽

Cited By ~ 7

Author(s):

Bih-Show Lou ◽

Chih-Ho Lai ◽

Teng-Ping Chu ◽

Jang-Hsing Hsieh ◽

Chun-Ming Chen ◽

...

Keyword(s):

Gas Flow ◽

Treatment Time ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Gas Flow Rate ◽

Cold Atmospheric Plasma ◽

E Coli ◽

Antimicrobial Efficiency ◽

Ar Gas ◽

Sample Distance

Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections.

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The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes

Toxins ◽

10.3390/toxins11070390 ◽

2019 ◽

Vol 11 (7) ◽

pp. 390 ◽

Cited By ~ 2

Author(s):

Chunping Yang ◽

Tianxing Lv ◽

Bin Wang ◽

Xiaoyan Qiu ◽

Liya Luo ◽

...

Keyword(s):

Cell Cycle ◽

Mortality Rate ◽

Treatment Time ◽

Immune Defense ◽

Control Group ◽

Pomacea Canaliculata ◽

Abnormal Growth ◽

Cell Mortality ◽

Induced Apoptosis ◽

Damaging Effects

Pomacea canaliculata hemocytes are the main functional cells in the immune defense system, and hemocyte destruction disrupts the immune response mechanism of P. canaliculata, resulting in abnormal growth, development, reproduction, and even death. Our previous study found that Pedunsaponin A significantly affects P. canaliculata hemocyte structure. This study further investigated the damaging effects of Pedunsaponin A on P. canaliculata hemocytes. The cell mortality rate results showed that the hemocyte mortality was significantly increased after treatment with Pedunsaponin A, and the mortality rate exhibited a significant positive correlation with treatment time and dose. The membrane potential results showed that the cell membranes of P. canaliculata hemocytes exhibited time-dependent membrane depolarization after 40 mg/L Pedunsaponin A treatment. At 36 h, the cell depolarization rate in the Pedunsaponin A treatment group was 41.43%, which was significantly greater than the control group (6.24%). The cytoskeleton results showed that Pedunsaponin A led to disordered and dispersed arrangement of microfilaments and changes in the cytoskeletal structure. The apoptosis and cell cycle results showed that Pedunsaponin A induced apoptosis and influenced the cell cycle to some extent. These results showed that the cell membrane and cytoskeleton of P. canaliculata hemocytes were damaged after treatment with Pedunsaponin A, which led to an increase in cell mortality, dysfunction, cell cycle abnormalities and apoptosis. This study provides a foundation for further identification of the site of Pedunsaponin A activity on hemocytes.

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The Use of Cold Atmospheric Plasma Device in Flap Elevation

Plastic & Reconstructive Surgery Global Open ◽

10.1097/gox.0000000000002815 ◽

2020 ◽

Vol Publish Ahead of Print ◽

Author(s):

Massimo Pinelli ◽

Marta Starnoni ◽

Giorgio De Santis

Keyword(s):

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Plasma Device ◽

Flap Elevation

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Cold Atmospheric Plasma Selectively Induces G0/G1 Cell Cycle Arrest and Apoptosis in AR-Independent Prostate Cancer Cells

10.21203/rs.3.rs-49986/v1 ◽

2020 ◽

Author(s):

Meng Ning ◽

Zhifa Zhang ◽

Lihui Yu ◽

Peiyu Han ◽

xiaofeng Dai

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Androgen Receptor ◽

Cancer Cells ◽

Atmospheric Plasma ◽

Prostate Cancer Cells ◽

Cold Atmospheric Plasma ◽

Prostate Cancers ◽

And Migration ◽

Physical Plasma

Abstract BackgroundAndrogen receptor-independent prostate cancers do not respond to androgen blockage therapies and suffer from high recurrence rate. We aim to contribute to the establishment of novel therapeutic approaches against such malignancies.Methods We examined whether and how cold atmospheric plasma delivers selectivity against AR-independent prostate cancers using human normal epithelial prostatic cells PNT1A and AR-negative DU145 prostate cancer cells.ResultsWe show that cold atmospheric plasma could selectively halt cell proliferation and migration in androgen receptor-independent cells as a result of induced cell apoptosis and G0/G1 stage cell cycle arrest, and such outcomes were achieved through modulations on the MAPK and NF-kB pathways in response to physical plasma induced intracellular redox level. ConclusionOur study reports cold atmospheric plasma induced reduction on the proliferation and migration of androgen receptor-independent prostate cancer cells that offers novel therapeutic insights on the treatment of such cancers, and provides the first evidence on physical plasma induced cell cycle G0/G1 stage arrest that warrants the exploration on the synergistic use of cold atmospheric plasma and drugs such as chemotherapies in eradicating such cancer cells.

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