scholarly journals Evaluation of Qualitative Traits In Modified Iranian Red Rey Onion And Comparison Genetic Resistance With Primary Mass Selection And Red Azar-Shahr cv To Fusarium Oxysporum Using Laboratory And Molecular Markers

Author(s):  
Sayyedeh Mahsan Taghi Shokrgozar ◽  
Mohsen Khodadadi ◽  
Vahid Abdossi ◽  
Vahid Zarrin nia ◽  
Ramin Hajiyan far

Abstract The aim of this study is to evaluate the storage and qualitative feature of modified Red Rey Iranian Onion. Firstly, the modified population was obtained by the selection of superior bulbs, cultivation, its self-pollination and consequently the identification of the best families and implement open pollination between them. In next level, the Red Rey Iranian modified with basic population and Red Azar-shahr cultivar (comparative) was crossed. Our results showed that the selection procedure has leading to improvement in variety of traits in population. Also, the modified Red Rey is significantly superior to the base mass in qualitative traits such as: bulb stiffness, bulb dry matter, TSS, total sugar and glucose; So that the percentage of dry bulb content increased from 10.4% in the basal mass to 11.1% in the modified Red Rey; while spouring and rotting, minerals, and dry matter, vitamin C and fructose-reducing sugar was not affected by genotype. In the second step, resistances to Fusarium wilt disease (laboratory and molecular markers) were evaluated. Based on the results of phenotypic evaluation, the modified Red Rey had the lowest rate and level of infection and the highest score. According to the results of genotypic evaluation, there is a very high genetic affinity between resistant and susceptible cultivars.

2018 ◽  
Vol 18(33) (2) ◽  
pp. 183-192 ◽  
Author(s):  
Karol Kukuła ◽  
Lidia Luty

The aim of the article is to present the technique of selection of the method of the linear ordering of objects due to the level of a complex phenomenon, which is the status of waste management in voivodeships in Poland. For this purpose there were selected diagnostic variables describing the analyzed phenomenon and linear ordering methods were selected. Then, using the selection procedure for ordering methods, it was chosen the method of construction of a synthetic variable, that allowed to build ranking of voivodeships due to the level of the analyzed phenomenon. Then the voivodeships were divided into four groups: very high, high, average and low level of the discussed phenomenon. The research showed that the selection of procedure for the construction of synthetic variable affects the ranking of tested objects. The obtained ranking allows to conclude that waste management in Poland is regionally differentiated.


Methodology ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 177-188 ◽  
Author(s):  
Martin Schultze ◽  
Michael Eid

Abstract. In the construction of scales intended for the use in cross-cultural studies, the selection of items needs to be guided not only by traditional criteria of item quality, but has to take information about the measurement invariance of the scale into account. We present an approach to automated item selection which depicts the process as a combinatorial optimization problem and aims at finding a scale which fulfils predefined target criteria – such as measurement invariance across cultures. The search for an optimal solution is performed using an adaptation of the [Formula: see text] Ant System algorithm. The approach is illustrated using an application to item selection for a personality scale assuming measurement invariance across multiple countries.


Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.


Author(s):  
N. I. Kashevarov ◽  
R. I. Polyudina ◽  
I. N. Kazarinova ◽  
D. А. Potapov

A new cultivar of smooth bromegrass (Bromopsis inermis Leyss) Flagman was developed by methods of mass selection and polycross. Breeding and wild forms of various ecological and geographical origins are used as an initial material. The authors of the cultivar: Kazarinova I.N., Polyudina R.I., Straub A.A., Gomasco S.K. Studies were conducted on the Central experimental base of the Siberian research Institute of fodder crops of the Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, located in the forest-steppe zone of Western Siberia (Novosibirsk region, Novosibirsk district, Krasnoobsk). The cultivar is mid-ripening: the period from the beginning of spring aftergrowing to mowing ripeness is 63-75 days and to full maturing of seeds is 95-111 days. The yield of dry matter is 8.3 t/ha, which exceeds the standard by 8%, seed yield - 0.62 t/ha, higher than the standard by 28%. The dry matter yield of the cultivar Flagman for the fourth year of use exceeded the standard by 23% and reached to 11.4 t/ha. The 1000 seeds weight is 3.0-3.4 g. The plant height is 90-140 cm. Tilling capacity is up to 40 stems per tuft. Foliage varies from 32 to 50% depending on the age of the grass and environment conditions. The resistance of the cultivar to brown rust and helminthosporiosis is higher than of the standard. The copyright certificate No. 71916 and patent No. 9653 were received.


2016 ◽  
Vol 2 (91) ◽  
pp. 57-62
Author(s):  
O.L. Kyrylesko

Influence of top-dressing is considered in the article, norms and terms of sowing on of winter-annual rape. The assessment conducted by the yield of green mass and seeds, output capacity by about 1 hectare of dry matter, feed units and digestible protein, the number of dead plants and density of herbage. Established that hardiness and productivity of winter rape can be enhanced through the use of farming practices as: by creating a moderate density of herbage, using optimal terms of planting and doses of mineral fertilizers, selection of predecessors and careful preparation of the soil ect. The mechanism of influence of agrotechnical receptions is exposed on of winter-annual rape through determination in roots before the offensive of the winter of separate biochemical indexes (sugar, starch, to protein).


2004 ◽  
Vol 155 (5) ◽  
pp. 142-145 ◽  
Author(s):  
Claudio Defila

The record-breaking heatwave of 2003 also had an impact on the vegetation in Switzerland. To examine its influences seven phenological late spring and summer phases were evaluated together with six phases in the autumn from a selection of stations. 30% of the 122 chosen phenological time series in late spring and summer phases set a new record (earliest arrival). The proportion of very early arrivals is very high and the mean deviation from the norm is between 10 and 20 days. The situation was less extreme in autumn, where 20% of the 103 time series chosen set a new record. The majority of the phenological arrivals were found in the class «normal» but the class«very early» is still well represented. The mean precocity lies between five and twenty days. As far as the leaf shedding of the beech is concerned, there was even a slight delay of around six days. The evaluation serves to show that the heatwave of 2003 strongly influenced the phenological events of summer and spring.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Colin Griesbach ◽  
Benjamin Säfken ◽  
Elisabeth Waldmann

Abstract Gradient boosting from the field of statistical learning is widely known as a powerful framework for estimation and selection of predictor effects in various regression models by adapting concepts from classification theory. Current boosting approaches also offer methods accounting for random effects and thus enable prediction of mixed models for longitudinal and clustered data. However, these approaches include several flaws resulting in unbalanced effect selection with falsely induced shrinkage and a low convergence rate on the one hand and biased estimates of the random effects on the other hand. We therefore propose a new boosting algorithm which explicitly accounts for the random structure by excluding it from the selection procedure, properly correcting the random effects estimates and in addition providing likelihood-based estimation of the random effects variance structure. The new algorithm offers an organic and unbiased fitting approach, which is shown via simulations and data examples.


1951 ◽  
Vol 41 (3) ◽  
pp. 271-281 ◽  
Author(s):  
E. S. Bunting ◽  
G. E. Blackman

Between 1942 and 1950, some thirty field experiments have been carried out in the southern half of England to assess the potential value, either for grain or forage production, of seventeen openpollinated flint or dent maizes together with twentynine single or double hybrids of American or Canadian origin.Early-maturing flint varieties will consistently ripen grain, but before mechanical threshing or storage, the cobs require drying. Sibthorp, a mass selection made from an unknown German variety, is the earliest and most productive flint maize so far tested, and in the experiments has yielded as much as 39 cwt. of grain per acre with an average of 24 cwt. The earliest American hybrids, i.e. those with a U.S.A. rating of 80 days from sowing to maturity, give very high yields of grain in favourable seasons. Within the group Wisconsin 240–275, a yield level equal to or exceeding 50 cwt./acre has on occasion been recorded. On the other hand, in the most unfavourable years, such hybrids just failed to produce ripe cobs.Attempts to maintain sixty-five parent inlines of the earliest hybrids have largely failed. However, many of the parent single crosses have matured, and the production on a field scale of the double-cross seed of both Wisconsin 240 and 255 has been carried out.Spacing experiments indicate that for optimum grain production a density of 6 plants/sq.yd. is required for both flint varieties and the earliest hybrids. A spatial arrangement of individual plants is to be preferred to that of groups or hills.American hybrids, in the class of ‘90 days’ to maturity, will in all but the most unfavourable seasons reach the ‘early-dent’ stage of the grain before the incidence of autumn frosts. Yields of dry matter of plants harvested in this phase have ranged from 30 to 85 cwt. of dry matter per acre. In these trials, the plant density was standardized at 4 plants/sq.yd. and higher densities may be demanded for optimal yields.The ratio of the ‘ear’ (that is, the cob, immature grain and enclosing leaf sheaths) to the total shoot weight at harvest varies greatly with the variety or hybrid. With White Horsetooth, the usual variety grown for fodder in England, no cobs are formed before the plants are killed by frost, while with the early hybrids, the ear may be half the weight of the whole shoot.The earliest variety Sibthorp from an early May sowing takes approximately 70–80 days to reach full anthesis, while ‘80-day’ American hybrids are a week later. From sowing to full maturity the period in England is from 140 to 160 days, thus compared to conditions in Minnesota the period is nearly twice as long. Because of the much slower rate of development and because of the humidity of English autumns, it is concluded that until the date of maturity can be advanced some 14 days, grain production on a field scale is not yet feasible. On the other hand, many of the American hybrids are well fitted to the production of silage. The greatest drawback to the introduction of such hybrids is the liability of the seed and seedlings to be attacked by rooks.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.


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